Fiber-substituted Conditionally Replicating Adenovirus for Oncolysis of Human Renal Carcinoma Cells
Adenovirus vectors have lately been highlighted in gene therapies. We investigated the oncolytic effects of a chimeric adenovirus type 5 (Ad5) with replacement of Ad5 fiber knob with adenovirus type 35 (Ad35) fiber knob (Ad5F35) on human renal cell carcinoma (RCC). The conditionally replicating Ad5F...
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| Veröffentlicht in: | Anticancer research 2012-07, Vol.32 (7), p.2985-2989 |
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| container_title | Anticancer research |
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| creator | NAGAYA, Hisao TAGAWA, Masatoshi HIWASA, Kazuhiro TERAO, Shuji KANNO, Takeshi NISHIZAKI, Tomoyuki GOTOH, Akinobu |
| description | Adenovirus vectors have lately been highlighted in gene therapies. We investigated the oncolytic effects of a chimeric adenovirus type 5 (Ad5) with replacement of Ad5 fiber knob with adenovirus type 35 (Ad35) fiber knob (Ad5F35) on human renal cell carcinoma (RCC).
The conditionally replicating Ad5F35 vector was constructed and infected into RCC cell lines 786-O, ACHN, and RCC4-VHL. For these cells, reverse transcription-polymerase chain reaction and western blotting were carried out and the cell viability was assayed.
In all RCC cell lines, it was found that CD46, a cell surface target of Ad35, was well-expressed, while coxsackie and adenovirus receptor (CAR), a cell surface target of Ad5, was considerably less expressed. The Ad5F35 vector induced oncolysis of RCC cells, with significantly higher efficacy as compared with that for the Ad5 vector.
Ad5F35 vector could be a candidate for promising gene therapy of human RCC. |
| format | Article |
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The conditionally replicating Ad5F35 vector was constructed and infected into RCC cell lines 786-O, ACHN, and RCC4-VHL. For these cells, reverse transcription-polymerase chain reaction and western blotting were carried out and the cell viability was assayed.
In all RCC cell lines, it was found that CD46, a cell surface target of Ad35, was well-expressed, while coxsackie and adenovirus receptor (CAR), a cell surface target of Ad5, was considerably less expressed. The Ad5F35 vector induced oncolysis of RCC cells, with significantly higher efficacy as compared with that for the Ad5 vector.
Ad5F35 vector could be a candidate for promising gene therapy of human RCC.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 22753762</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Adenoviridae - genetics ; Adenoviridae - physiology ; Adenovirus ; Adenovirus E1 Proteins - genetics ; Biological and medical sciences ; Carcinoma, Renal Cell - therapy ; Carcinoma, Renal Cell - virology ; CD46 antigen ; Cell Line, Tumor ; Cell surface ; Coxsackie and Adenovirus Receptor-Like Membrane Protein ; Cytokines - genetics ; Expression vectors ; Fibers ; Gene therapy ; Genetic Therapy - methods ; Genetic Vectors ; HEK293 Cells ; Humans ; Kidney Neoplasms - therapy ; Kidney Neoplasms - virology ; Kidneys ; Medical sciences ; Membrane Cofactor Protein - biosynthesis ; Membrane Cofactor Protein - genetics ; Nephrology. Urinary tract diseases ; Oncolysis ; Oncolytic Virotherapy - methods ; Promoter Regions, Genetic ; Receptors, Virus - biosynthesis ; Receptors, Virus - genetics ; renal cell carcinoma ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; Tumors ; Tumors of the urinary system ; Virus Replication ; Western blotting</subject><ispartof>Anticancer research, 2012-07, Vol.32 (7), p.2985-2989</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26084395$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22753762$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NAGAYA, Hisao</creatorcontrib><creatorcontrib>TAGAWA, Masatoshi</creatorcontrib><creatorcontrib>HIWASA, Kazuhiro</creatorcontrib><creatorcontrib>TERAO, Shuji</creatorcontrib><creatorcontrib>KANNO, Takeshi</creatorcontrib><creatorcontrib>NISHIZAKI, Tomoyuki</creatorcontrib><creatorcontrib>GOTOH, Akinobu</creatorcontrib><title>Fiber-substituted Conditionally Replicating Adenovirus for Oncolysis of Human Renal Carcinoma Cells</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Adenovirus vectors have lately been highlighted in gene therapies. We investigated the oncolytic effects of a chimeric adenovirus type 5 (Ad5) with replacement of Ad5 fiber knob with adenovirus type 35 (Ad35) fiber knob (Ad5F35) on human renal cell carcinoma (RCC).
The conditionally replicating Ad5F35 vector was constructed and infected into RCC cell lines 786-O, ACHN, and RCC4-VHL. For these cells, reverse transcription-polymerase chain reaction and western blotting were carried out and the cell viability was assayed.
In all RCC cell lines, it was found that CD46, a cell surface target of Ad35, was well-expressed, while coxsackie and adenovirus receptor (CAR), a cell surface target of Ad5, was considerably less expressed. The Ad5F35 vector induced oncolysis of RCC cells, with significantly higher efficacy as compared with that for the Ad5 vector.
Ad5F35 vector could be a candidate for promising gene therapy of human RCC.</description><subject>Adenoviridae - genetics</subject><subject>Adenoviridae - physiology</subject><subject>Adenovirus</subject><subject>Adenovirus E1 Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Carcinoma, Renal Cell - therapy</subject><subject>Carcinoma, Renal Cell - virology</subject><subject>CD46 antigen</subject><subject>Cell Line, Tumor</subject><subject>Cell surface</subject><subject>Coxsackie and Adenovirus Receptor-Like Membrane Protein</subject><subject>Cytokines - genetics</subject><subject>Expression vectors</subject><subject>Fibers</subject><subject>Gene therapy</subject><subject>Genetic Therapy - methods</subject><subject>Genetic Vectors</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Kidney Neoplasms - therapy</subject><subject>Kidney Neoplasms - virology</subject><subject>Kidneys</subject><subject>Medical sciences</subject><subject>Membrane Cofactor Protein - biosynthesis</subject><subject>Membrane Cofactor Protein - genetics</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Oncolysis</subject><subject>Oncolytic Virotherapy - methods</subject><subject>Promoter Regions, Genetic</subject><subject>Receptors, Virus - biosynthesis</subject><subject>Receptors, Virus - genetics</subject><subject>renal cell carcinoma</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Tumors</subject><subject>Tumors of the urinary system</subject><subject>Virus Replication</subject><subject>Western blotting</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0M1KxDAUBeAgijOOvoJkI7gp5KdJ2uVQ1BEGBkTXJU1SiaRJTVJh3t6AI25dXS5853K4Z2CNRYsrwSg6B2tEGKoEQmwFrlL6QIjztqGXYEVIEYKTNVCPdjCxSsuQss1LNhp2wWubbfDSuSN8MbOzSmbr3-FWGx--bFwSHEOEB6-COyabYBjhbpmkL7qkYCejsj5MEnbGuXQNLkbpkrk5zQ14e3x47XbV_vD03G331UxEnStd61JJY4GpaKliDTdcaUZpWYYBE4nFoCgblRSibgRmjTBkUNhIJJnglG7A_c_dOYbPxaTcTzap0kB6E5bUY0RJ0_KW_ocSSlrBWV3o7Ykuw2R0P0c7yXjsf39YwN0JyKSkG6P0yqY_x1FT05bRb_BUe0U</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>NAGAYA, Hisao</creator><creator>TAGAWA, Masatoshi</creator><creator>HIWASA, Kazuhiro</creator><creator>TERAO, Shuji</creator><creator>KANNO, Takeshi</creator><creator>NISHIZAKI, Tomoyuki</creator><creator>GOTOH, Akinobu</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20120701</creationdate><title>Fiber-substituted Conditionally Replicating Adenovirus for Oncolysis of Human Renal Carcinoma Cells</title><author>NAGAYA, Hisao ; TAGAWA, Masatoshi ; HIWASA, Kazuhiro ; TERAO, Shuji ; KANNO, Takeshi ; NISHIZAKI, Tomoyuki ; GOTOH, Akinobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p274t-d4d762d1713793c586e6cd53393cbb12a17bc35fca774871587e2bc1ea0a57633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adenoviridae - genetics</topic><topic>Adenoviridae - physiology</topic><topic>Adenovirus</topic><topic>Adenovirus E1 Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Renal Cell - therapy</topic><topic>Carcinoma, Renal Cell - virology</topic><topic>CD46 antigen</topic><topic>Cell Line, Tumor</topic><topic>Cell surface</topic><topic>Coxsackie and Adenovirus Receptor-Like Membrane Protein</topic><topic>Cytokines - genetics</topic><topic>Expression vectors</topic><topic>Fibers</topic><topic>Gene therapy</topic><topic>Genetic Therapy - methods</topic><topic>Genetic Vectors</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Kidney Neoplasms - therapy</topic><topic>Kidney Neoplasms - virology</topic><topic>Kidneys</topic><topic>Medical sciences</topic><topic>Membrane Cofactor Protein - biosynthesis</topic><topic>Membrane Cofactor Protein - genetics</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Oncolysis</topic><topic>Oncolytic Virotherapy - methods</topic><topic>Promoter Regions, Genetic</topic><topic>Receptors, Virus - biosynthesis</topic><topic>Receptors, Virus - genetics</topic><topic>renal cell carcinoma</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Tumors</topic><topic>Tumors of the urinary system</topic><topic>Virus Replication</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NAGAYA, Hisao</creatorcontrib><creatorcontrib>TAGAWA, Masatoshi</creatorcontrib><creatorcontrib>HIWASA, Kazuhiro</creatorcontrib><creatorcontrib>TERAO, Shuji</creatorcontrib><creatorcontrib>KANNO, Takeshi</creatorcontrib><creatorcontrib>NISHIZAKI, Tomoyuki</creatorcontrib><creatorcontrib>GOTOH, Akinobu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NAGAYA, Hisao</au><au>TAGAWA, Masatoshi</au><au>HIWASA, Kazuhiro</au><au>TERAO, Shuji</au><au>KANNO, Takeshi</au><au>NISHIZAKI, Tomoyuki</au><au>GOTOH, Akinobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fiber-substituted Conditionally Replicating Adenovirus for Oncolysis of Human Renal Carcinoma Cells</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2012-07-01</date><risdate>2012</risdate><volume>32</volume><issue>7</issue><spage>2985</spage><epage>2989</epage><pages>2985-2989</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Adenovirus vectors have lately been highlighted in gene therapies. We investigated the oncolytic effects of a chimeric adenovirus type 5 (Ad5) with replacement of Ad5 fiber knob with adenovirus type 35 (Ad35) fiber knob (Ad5F35) on human renal cell carcinoma (RCC).
The conditionally replicating Ad5F35 vector was constructed and infected into RCC cell lines 786-O, ACHN, and RCC4-VHL. For these cells, reverse transcription-polymerase chain reaction and western blotting were carried out and the cell viability was assayed.
In all RCC cell lines, it was found that CD46, a cell surface target of Ad35, was well-expressed, while coxsackie and adenovirus receptor (CAR), a cell surface target of Ad5, was considerably less expressed. The Ad5F35 vector induced oncolysis of RCC cells, with significantly higher efficacy as compared with that for the Ad5 vector.
Ad5F35 vector could be a candidate for promising gene therapy of human RCC.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>22753762</pmid><tpages>5</tpages></addata></record> |
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| subjects | Adenoviridae - genetics Adenoviridae - physiology Adenovirus Adenovirus E1 Proteins - genetics Biological and medical sciences Carcinoma, Renal Cell - therapy Carcinoma, Renal Cell - virology CD46 antigen Cell Line, Tumor Cell surface Coxsackie and Adenovirus Receptor-Like Membrane Protein Cytokines - genetics Expression vectors Fibers Gene therapy Genetic Therapy - methods Genetic Vectors HEK293 Cells Humans Kidney Neoplasms - therapy Kidney Neoplasms - virology Kidneys Medical sciences Membrane Cofactor Protein - biosynthesis Membrane Cofactor Protein - genetics Nephrology. Urinary tract diseases Oncolysis Oncolytic Virotherapy - methods Promoter Regions, Genetic Receptors, Virus - biosynthesis Receptors, Virus - genetics renal cell carcinoma RNA, Messenger - biosynthesis RNA, Messenger - genetics Tumors Tumors of the urinary system Virus Replication Western blotting |
| title | Fiber-substituted Conditionally Replicating Adenovirus for Oncolysis of Human Renal Carcinoma Cells |
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