Electrochemical based detection of microRNA, mir21 in breast cancer cells

In this work, a novel electrochemical microRNA (miRNA) detection method based on enzyme amplified biosensing of mir21 from cell lysate of total RNA was demonstrated. The proposed enzymatic detection method was detailed and compared with the conventional guanine oxidation based assay in terms of dete...

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Veröffentlicht in:	Biosensors & bioelectronics 2012-10, Vol.38 (1), p.195-201
Hauptverfasser:	Kilic, Tugba, Topkaya, Seda Nur, Ozkan Ariksoysal, Dilsat, Ozsoz, Mehmet, Ballar, Petek, Erac, Yasemin, Gozen, Oguz
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline phosphatase Alkaline Phosphatase - metabolism Base Sequence Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotechnology Breast - cytology Breast - metabolism Breast cancer Breast Neoplasms - diagnosis Breast Neoplasms - genetics Electrochemical biosensor Electrochemical Techniques - instrumentation Electrochemical Techniques - methods Electrodes Enzymatic biosensor Enzyme Assays - instrumentation Enzyme Assays - methods Female Fundamental and applied biological sciences. Psychology Graphite - chemistry Guanine - metabolism Humans Limit of Detection Methods. Procedures. Technologies MicroRNAs - analysis MicroRNAs - genetics MicroRNAs - isolation & purification mir21 miRNA Nucleic Acid Hybridization Oxidation-Reduction Succinimides Various methods and equipments
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