Molecular cloning, characterization and expression analysis of tumor necrosis factor receptor-associated factor 3 (TRAF3) from pearl oyster Pinctada fucata
TRAF3 is a highly versatile regulator that negatively regulates JNK and alternative nuclear factor-κB signalling, but positively controls type I interferon production. To investigate TRAF3 function in innate immune responses among invertebrate especially mollusk, we characterized TRAF3 (PfTRAF3) fro...
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| Veröffentlicht in: | Fish & shellfish immunology 2012-09, Vol.33 (3), p.652-658 |
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| container_title | Fish & shellfish immunology |
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| creator | Huang, Xian-De Liu, Wen-Guang Guan, Yun-Yan Shi, Yu Wang, Qi Zhao, Mi Wu, Shan-Zeng He, Mao-Xian |
| description | TRAF3 is a highly versatile regulator that negatively regulates JNK and alternative nuclear factor-κB signalling, but positively controls type I interferon production. To investigate TRAF3 function in innate immune responses among invertebrate especially mollusk, we characterized TRAF3 (PfTRAF3) from pearl oyster Pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. PfTRAF3 cDNA is 2261 bp with an open reading frame of 1623 bp encoding a putative protein of 541 amino acids. The deduced PfTRAF3 contains a RING finger domain, two TRAF domains with zinc finger domains and a conserved C-terminal meprin and TRAF homology (MATH) domain. Comparison and phylogenetic analysis revealed that PfTRAF3 from mollusk shared a higher identity with Ciona intestinalis TRAF3 from urochordata, Branchiostoma belcheri TRAF3 from cephalochordate, and even TRAF3 from vertebrate than with insect homologues. Furthermore, gene expression analyses suggested that PfTRAF3 was involved in the immune response to Vibrio alginolyticus.
► Tumor necrosis factor receptor-associated factor 3 (TRAF3) from Pinctada fucata was cloned. ► PfTRAF3 shared a lower identity with insect homologues. ► PfTRAF3 was involved in the immune response to Vibrio alginolyticus |
| doi_str_mv | 10.1016/j.fsi.2012.06.026 |
| format | Article |
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► Tumor necrosis factor receptor-associated factor 3 (TRAF3) from Pinctada fucata was cloned. ► PfTRAF3 shared a lower identity with insect homologues. ► PfTRAF3 was involved in the immune response to Vibrio alginolyticus</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2012.06.026</identifier><identifier>PMID: 22796485</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; China ; Cloning, Molecular ; DNA, Complementary - genetics ; Gene Expression Profiling ; Gene Expression Regulation ; Immune response ; Immunity, Innate ; Molecular Sequence Data ; Organ Specificity ; Pearl oyster ; PfTRAF3 ; Phylogeny ; Pinctada - genetics ; Pinctada - immunology ; Pinctada - metabolism ; Pinctada - microbiology ; Real-Time Polymerase Chain Reaction ; RNA, Messenger - analysis ; Sequence Alignment ; Sequence Homology, Amino Acid ; Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - chemistry ; Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - genetics ; Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - immunology ; Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - metabolism ; Vibrio alginolyticus ; Vibrio alginolyticus - physiology</subject><ispartof>Fish & shellfish immunology, 2012-09, Vol.33 (3), p.652-658</ispartof><rights>2012 Elsevier Ltd</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-b7cea5b060a922bdefb089e038d55fc3bdb4413397126ec49c40cfa2788fe2083</citedby><cites>FETCH-LOGICAL-c419t-b7cea5b060a922bdefb089e038d55fc3bdb4413397126ec49c40cfa2788fe2083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fsi.2012.06.026$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22796485$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Xian-De</creatorcontrib><creatorcontrib>Liu, Wen-Guang</creatorcontrib><creatorcontrib>Guan, Yun-Yan</creatorcontrib><creatorcontrib>Shi, Yu</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Zhao, Mi</creatorcontrib><creatorcontrib>Wu, Shan-Zeng</creatorcontrib><creatorcontrib>He, Mao-Xian</creatorcontrib><title>Molecular cloning, characterization and expression analysis of tumor necrosis factor receptor-associated factor 3 (TRAF3) from pearl oyster Pinctada fucata</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>TRAF3 is a highly versatile regulator that negatively regulates JNK and alternative nuclear factor-κB signalling, but positively controls type I interferon production. To investigate TRAF3 function in innate immune responses among invertebrate especially mollusk, we characterized TRAF3 (PfTRAF3) from pearl oyster Pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. PfTRAF3 cDNA is 2261 bp with an open reading frame of 1623 bp encoding a putative protein of 541 amino acids. The deduced PfTRAF3 contains a RING finger domain, two TRAF domains with zinc finger domains and a conserved C-terminal meprin and TRAF homology (MATH) domain. Comparison and phylogenetic analysis revealed that PfTRAF3 from mollusk shared a higher identity with Ciona intestinalis TRAF3 from urochordata, Branchiostoma belcheri TRAF3 from cephalochordate, and even TRAF3 from vertebrate than with insect homologues. Furthermore, gene expression analyses suggested that PfTRAF3 was involved in the immune response to Vibrio alginolyticus.
► Tumor necrosis factor receptor-associated factor 3 (TRAF3) from Pinctada fucata was cloned. ► PfTRAF3 shared a lower identity with insect homologues. ► PfTRAF3 was involved in the immune response to Vibrio alginolyticus</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>China</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - genetics</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Immune response</subject><subject>Immunity, Innate</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity</subject><subject>Pearl oyster</subject><subject>PfTRAF3</subject><subject>Phylogeny</subject><subject>Pinctada - genetics</subject><subject>Pinctada - immunology</subject><subject>Pinctada - metabolism</subject><subject>Pinctada - microbiology</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - chemistry</subject><subject>Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - genetics</subject><subject>Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - immunology</subject><subject>Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - metabolism</subject><subject>Vibrio alginolyticus</subject><subject>Vibrio alginolyticus - physiology</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1TAQhSMEoj_wAGyQl0VqwthxnESsqqoFpFZFqKytiTMGXyVxsBPE5VX6sji6LUtWnhmfc6zxl2VvOBQcuHq_K2x0hQAuClAFCPUsO-bQVnnbyvr5VleQSyWbo-wkxh0AqFLBy-xIiLpN4-o4e7j1A5l1wMDM4Cc3fT9n5gcGNAsF9wcX5yeGU8_o9xwoxkOLwz66yLxlyzr6wCYywW8Tm3ypD2RoTkWOMXrjcKH-6apkZ_dfL67Ld8wGP7KZMAzM72N6jn1xk1mwR2ZXgwu-yl5YHCK9fjxPs2_XV_eXn_Kbu4-fLy9uciN5u-RdbQirDhRgK0TXk-2gaQnKpq8qa8qu76TkZdnWXCgysjUSjEVRN40lAU15mp0dcufgf64UFz26aGgYcCK_Rs2h5FxxIWWS8oN02zcGsnoObsSwTyK9MdE7nZjojYkGpROT5Hn7GL92I_X_HE8QkuDDQUBpyV-Ogo7G0WSod-kjF91795_4v7MMn7k</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Huang, Xian-De</creator><creator>Liu, Wen-Guang</creator><creator>Guan, Yun-Yan</creator><creator>Shi, Yu</creator><creator>Wang, Qi</creator><creator>Zhao, Mi</creator><creator>Wu, Shan-Zeng</creator><creator>He, Mao-Xian</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120901</creationdate><title>Molecular cloning, characterization and expression analysis of tumor necrosis factor receptor-associated factor 3 (TRAF3) from pearl oyster Pinctada fucata</title><author>Huang, Xian-De ; Liu, Wen-Guang ; Guan, Yun-Yan ; Shi, Yu ; Wang, Qi ; Zhao, Mi ; Wu, Shan-Zeng ; He, Mao-Xian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-b7cea5b060a922bdefb089e038d55fc3bdb4413397126ec49c40cfa2788fe2083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>China</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - genetics</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Immune response</topic><topic>Immunity, Innate</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity</topic><topic>Pearl oyster</topic><topic>PfTRAF3</topic><topic>Phylogeny</topic><topic>Pinctada - genetics</topic><topic>Pinctada - immunology</topic><topic>Pinctada - metabolism</topic><topic>Pinctada - microbiology</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - chemistry</topic><topic>Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - genetics</topic><topic>Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - immunology</topic><topic>Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - metabolism</topic><topic>Vibrio alginolyticus</topic><topic>Vibrio alginolyticus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Xian-De</creatorcontrib><creatorcontrib>Liu, Wen-Guang</creatorcontrib><creatorcontrib>Guan, Yun-Yan</creatorcontrib><creatorcontrib>Shi, Yu</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Zhao, Mi</creatorcontrib><creatorcontrib>Wu, Shan-Zeng</creatorcontrib><creatorcontrib>He, Mao-Xian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Xian-De</au><au>Liu, Wen-Guang</au><au>Guan, Yun-Yan</au><au>Shi, Yu</au><au>Wang, Qi</au><au>Zhao, Mi</au><au>Wu, Shan-Zeng</au><au>He, Mao-Xian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning, characterization and expression analysis of tumor necrosis factor receptor-associated factor 3 (TRAF3) from pearl oyster Pinctada fucata</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>33</volume><issue>3</issue><spage>652</spage><epage>658</epage><pages>652-658</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>TRAF3 is a highly versatile regulator that negatively regulates JNK and alternative nuclear factor-κB signalling, but positively controls type I interferon production. To investigate TRAF3 function in innate immune responses among invertebrate especially mollusk, we characterized TRAF3 (PfTRAF3) from pearl oyster Pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. PfTRAF3 cDNA is 2261 bp with an open reading frame of 1623 bp encoding a putative protein of 541 amino acids. The deduced PfTRAF3 contains a RING finger domain, two TRAF domains with zinc finger domains and a conserved C-terminal meprin and TRAF homology (MATH) domain. Comparison and phylogenetic analysis revealed that PfTRAF3 from mollusk shared a higher identity with Ciona intestinalis TRAF3 from urochordata, Branchiostoma belcheri TRAF3 from cephalochordate, and even TRAF3 from vertebrate than with insect homologues. Furthermore, gene expression analyses suggested that PfTRAF3 was involved in the immune response to Vibrio alginolyticus.
► Tumor necrosis factor receptor-associated factor 3 (TRAF3) from Pinctada fucata was cloned. ► PfTRAF3 shared a lower identity with insect homologues. ► PfTRAF3 was involved in the immune response to Vibrio alginolyticus</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>22796485</pmid><doi>10.1016/j.fsi.2012.06.026</doi><tpages>7</tpages></addata></record> |
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| ispartof | Fish & shellfish immunology, 2012-09, Vol.33 (3), p.652-658 |
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| subjects | Amino Acid Sequence Animals Base Sequence China Cloning, Molecular DNA, Complementary - genetics Gene Expression Profiling Gene Expression Regulation Immune response Immunity, Innate Molecular Sequence Data Organ Specificity Pearl oyster PfTRAF3 Phylogeny Pinctada - genetics Pinctada - immunology Pinctada - metabolism Pinctada - microbiology Real-Time Polymerase Chain Reaction RNA, Messenger - analysis Sequence Alignment Sequence Homology, Amino Acid Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - chemistry Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - genetics Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - immunology Tumor Necrosis Factor Receptor-Associated Peptides and Proteins - metabolism Vibrio alginolyticus Vibrio alginolyticus - physiology |
| title | Molecular cloning, characterization and expression analysis of tumor necrosis factor receptor-associated factor 3 (TRAF3) from pearl oyster Pinctada fucata |
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