Proangiogenic immature myeloid cells populate the human placenta and their presence correlates with placental and birthweight
Objective To determine whether proangiogenic immature myeloid cells are present in human placentas. Study Design Biopsies were obtained from 61 placentas of term pregnancies. Percentage of CD45+ CD33+ LIN2− HLADR− immature myeloid cells of total CD45+ hematopoietic cells was determined by flow cytom...
Gespeichert in:
| Veröffentlicht in: | American journal of obstetrics and gynecology 2012-08, Vol.207 (2), p.141.e1-141.e5 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 141.e5 |
|---|---|
| container_issue | 2 |
| container_start_page | 141.e1 |
| container_title | American journal of obstetrics and gynecology |
| container_volume | 207 |
| creator | Mei-Dan, Elad, MD Fainaru, Ofer, MD, PhD Hantisteanu, Shay, Msc Shavit, Tal, MD Ellenbogen, Adrian, MD Hallak, Mordechai, MD |
| description | Objective To determine whether proangiogenic immature myeloid cells are present in human placentas. Study Design Biopsies were obtained from 61 placentas of term pregnancies. Percentage of CD45+ CD33+ LIN2− HLADR− immature myeloid cells of total CD45+ hematopoietic cells was determined by flow cytometry. Location of immature myeloid cells in the placenta was identified using confocal microscopy. The proangiogenic potential of immature myeloid cells was analyzed by endothelial tube formation. Results Immature myeloid cells comprise ∼25% of human placental CD45+ hematopoietic cells and infiltrate placentas in proximity of blood vessels. The percentage of immature myeloid cells correlated positively with placental weight (r 2 = 0.108, P = .01) and birthweight (r 2 = 0.087, P = .02). Endothelial tube formation was increased in the presence of immature myeloid cells as compared with the presence of CD45+ LIN2+ control cells. Conclusion Human placentas are populated by immature myeloid cells in the proximity of blood vessels. Consistent with their involvement in angiogenesis, immature myeloid cells accelerated endothelial tube formation. The presence of immature myeloid cells in pathologic pregnancies warrants further studies. |
| doi_str_mv | 10.1016/j.ajog.2012.05.025 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1030350829</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0002937812005881</els_id><sourcerecordid>1030350829</sourcerecordid><originalsourceid>FETCH-LOGICAL-c411t-4f965700ca45542fb947ca54c65f582c5bcc1a70e005eb9e6b60cda191b63c7e3</originalsourceid><addsrcrecordid>eNp9kU2L1TAUhoMoznX0D7iQLN20nqRNP0AEGfyCAQV1HdL09Da1bWqSznAX_ncT7zgLF67CCc974DwvIc8Z5AxY9WrK1WSPOQfGcxA5cPGAHBi0dVY1VfOQHACAZ21RNxfkifdTGnnLH5MLzpsSat4cyK8vzqr1aOwRV6OpWRYVdod0OeFsTU81zrOnm932WQWkYUQ67ota6TYrjWtQVK19-jaObg49rhqpts5h4j29NWG8Z-c_cGdcGG_RHMfwlDwa1Ozx2d17Sb6_f_ft6mN2_fnDp6u315kuGQtZObSVqAG0KoUo-dC1Za2VKHUlBtFwLTqtmaoBAQR2LVZdBbpXrGVdVegai0vy8rx3c_bnjj7Ixfh0mlrR7l4yKKAQ0PA2ovyMame9dzjIzZlFuVOEZNIuJ5m0y6RdgpBRewy9uNu_dwv295G_niPw-gxgvPLGoJNem-SqNw51kL01_9__5p-4nk3sS80_8IR-srtboz_JpI8Z-TU1nXpnPBppGlb8BvWyqzo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1030350829</pqid></control><display><type>article</type><title>Proangiogenic immature myeloid cells populate the human placenta and their presence correlates with placental and birthweight</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Mei-Dan, Elad, MD ; Fainaru, Ofer, MD, PhD ; Hantisteanu, Shay, Msc ; Shavit, Tal, MD ; Ellenbogen, Adrian, MD ; Hallak, Mordechai, MD</creator><creatorcontrib>Mei-Dan, Elad, MD ; Fainaru, Ofer, MD, PhD ; Hantisteanu, Shay, Msc ; Shavit, Tal, MD ; Ellenbogen, Adrian, MD ; Hallak, Mordechai, MD</creatorcontrib><description>Objective To determine whether proangiogenic immature myeloid cells are present in human placentas. Study Design Biopsies were obtained from 61 placentas of term pregnancies. Percentage of CD45+ CD33+ LIN2− HLADR− immature myeloid cells of total CD45+ hematopoietic cells was determined by flow cytometry. Location of immature myeloid cells in the placenta was identified using confocal microscopy. The proangiogenic potential of immature myeloid cells was analyzed by endothelial tube formation. Results Immature myeloid cells comprise ∼25% of human placental CD45+ hematopoietic cells and infiltrate placentas in proximity of blood vessels. The percentage of immature myeloid cells correlated positively with placental weight (r 2 = 0.108, P = .01) and birthweight (r 2 = 0.087, P = .02). Endothelial tube formation was increased in the presence of immature myeloid cells as compared with the presence of CD45+ LIN2+ control cells. Conclusion Human placentas are populated by immature myeloid cells in the proximity of blood vessels. Consistent with their involvement in angiogenesis, immature myeloid cells accelerated endothelial tube formation. The presence of immature myeloid cells in pathologic pregnancies warrants further studies.</description><identifier>ISSN: 0002-9378</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/j.ajog.2012.05.025</identifier><identifier>PMID: 22840728</identifier><language>eng</language><publisher>United States: Mosby, Inc</publisher><subject>Adult ; angiogenesis ; Antigens, CD - metabolism ; Antigens, Differentiation, Myelomonocytic - metabolism ; Birth Weight ; Endothelium, Vascular - embryology ; Female ; Flow Cytometry ; Humans ; immature myeloid cells ; Infant, Newborn ; Leukocyte Common Antigens - metabolism ; Microscopy, Confocal ; Myeloid Cells - metabolism ; Myeloid Cells - physiology ; Neovascularization, Physiologic ; Obstetrics and Gynecology ; Organ Size ; placenta ; Placenta - anatomy & histology ; Placenta - cytology ; Pregnancy ; Sialic Acid Binding Ig-like Lectin 3</subject><ispartof>American journal of obstetrics and gynecology, 2012-08, Vol.207 (2), p.141.e1-141.e5</ispartof><rights>Mosby, Inc.</rights><rights>2012 Mosby, Inc.</rights><rights>Copyright © 2012 Mosby, Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-4f965700ca45542fb947ca54c65f582c5bcc1a70e005eb9e6b60cda191b63c7e3</citedby><cites>FETCH-LOGICAL-c411t-4f965700ca45542fb947ca54c65f582c5bcc1a70e005eb9e6b60cda191b63c7e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ajog.2012.05.025$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22840728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mei-Dan, Elad, MD</creatorcontrib><creatorcontrib>Fainaru, Ofer, MD, PhD</creatorcontrib><creatorcontrib>Hantisteanu, Shay, Msc</creatorcontrib><creatorcontrib>Shavit, Tal, MD</creatorcontrib><creatorcontrib>Ellenbogen, Adrian, MD</creatorcontrib><creatorcontrib>Hallak, Mordechai, MD</creatorcontrib><title>Proangiogenic immature myeloid cells populate the human placenta and their presence correlates with placental and birthweight</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>Objective To determine whether proangiogenic immature myeloid cells are present in human placentas. Study Design Biopsies were obtained from 61 placentas of term pregnancies. Percentage of CD45+ CD33+ LIN2− HLADR− immature myeloid cells of total CD45+ hematopoietic cells was determined by flow cytometry. Location of immature myeloid cells in the placenta was identified using confocal microscopy. The proangiogenic potential of immature myeloid cells was analyzed by endothelial tube formation. Results Immature myeloid cells comprise ∼25% of human placental CD45+ hematopoietic cells and infiltrate placentas in proximity of blood vessels. The percentage of immature myeloid cells correlated positively with placental weight (r 2 = 0.108, P = .01) and birthweight (r 2 = 0.087, P = .02). Endothelial tube formation was increased in the presence of immature myeloid cells as compared with the presence of CD45+ LIN2+ control cells. Conclusion Human placentas are populated by immature myeloid cells in the proximity of blood vessels. Consistent with their involvement in angiogenesis, immature myeloid cells accelerated endothelial tube formation. The presence of immature myeloid cells in pathologic pregnancies warrants further studies.</description><subject>Adult</subject><subject>angiogenesis</subject><subject>Antigens, CD - metabolism</subject><subject>Antigens, Differentiation, Myelomonocytic - metabolism</subject><subject>Birth Weight</subject><subject>Endothelium, Vascular - embryology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>immature myeloid cells</subject><subject>Infant, Newborn</subject><subject>Leukocyte Common Antigens - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Myeloid Cells - metabolism</subject><subject>Myeloid Cells - physiology</subject><subject>Neovascularization, Physiologic</subject><subject>Obstetrics and Gynecology</subject><subject>Organ Size</subject><subject>placenta</subject><subject>Placenta - anatomy & histology</subject><subject>Placenta - cytology</subject><subject>Pregnancy</subject><subject>Sialic Acid Binding Ig-like Lectin 3</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2L1TAUhoMoznX0D7iQLN20nqRNP0AEGfyCAQV1HdL09Da1bWqSznAX_ncT7zgLF67CCc974DwvIc8Z5AxY9WrK1WSPOQfGcxA5cPGAHBi0dVY1VfOQHACAZ21RNxfkifdTGnnLH5MLzpsSat4cyK8vzqr1aOwRV6OpWRYVdod0OeFsTU81zrOnm932WQWkYUQ67ota6TYrjWtQVK19-jaObg49rhqpts5h4j29NWG8Z-c_cGdcGG_RHMfwlDwa1Ozx2d17Sb6_f_ft6mN2_fnDp6u315kuGQtZObSVqAG0KoUo-dC1Za2VKHUlBtFwLTqtmaoBAQR2LVZdBbpXrGVdVegai0vy8rx3c_bnjj7Ixfh0mlrR7l4yKKAQ0PA2ovyMame9dzjIzZlFuVOEZNIuJ5m0y6RdgpBRewy9uNu_dwv295G_niPw-gxgvPLGoJNem-SqNw51kL01_9__5p-4nk3sS80_8IR-srtboz_JpI8Z-TU1nXpnPBppGlb8BvWyqzo</recordid><startdate>20120801</startdate><enddate>20120801</enddate><creator>Mei-Dan, Elad, MD</creator><creator>Fainaru, Ofer, MD, PhD</creator><creator>Hantisteanu, Shay, Msc</creator><creator>Shavit, Tal, MD</creator><creator>Ellenbogen, Adrian, MD</creator><creator>Hallak, Mordechai, MD</creator><general>Mosby, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120801</creationdate><title>Proangiogenic immature myeloid cells populate the human placenta and their presence correlates with placental and birthweight</title><author>Mei-Dan, Elad, MD ; Fainaru, Ofer, MD, PhD ; Hantisteanu, Shay, Msc ; Shavit, Tal, MD ; Ellenbogen, Adrian, MD ; Hallak, Mordechai, MD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-4f965700ca45542fb947ca54c65f582c5bcc1a70e005eb9e6b60cda191b63c7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adult</topic><topic>angiogenesis</topic><topic>Antigens, CD - metabolism</topic><topic>Antigens, Differentiation, Myelomonocytic - metabolism</topic><topic>Birth Weight</topic><topic>Endothelium, Vascular - embryology</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>immature myeloid cells</topic><topic>Infant, Newborn</topic><topic>Leukocyte Common Antigens - metabolism</topic><topic>Microscopy, Confocal</topic><topic>Myeloid Cells - metabolism</topic><topic>Myeloid Cells - physiology</topic><topic>Neovascularization, Physiologic</topic><topic>Obstetrics and Gynecology</topic><topic>Organ Size</topic><topic>placenta</topic><topic>Placenta - anatomy & histology</topic><topic>Placenta - cytology</topic><topic>Pregnancy</topic><topic>Sialic Acid Binding Ig-like Lectin 3</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mei-Dan, Elad, MD</creatorcontrib><creatorcontrib>Fainaru, Ofer, MD, PhD</creatorcontrib><creatorcontrib>Hantisteanu, Shay, Msc</creatorcontrib><creatorcontrib>Shavit, Tal, MD</creatorcontrib><creatorcontrib>Ellenbogen, Adrian, MD</creatorcontrib><creatorcontrib>Hallak, Mordechai, MD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mei-Dan, Elad, MD</au><au>Fainaru, Ofer, MD, PhD</au><au>Hantisteanu, Shay, Msc</au><au>Shavit, Tal, MD</au><au>Ellenbogen, Adrian, MD</au><au>Hallak, Mordechai, MD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proangiogenic immature myeloid cells populate the human placenta and their presence correlates with placental and birthweight</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>2012-08-01</date><risdate>2012</risdate><volume>207</volume><issue>2</issue><spage>141.e1</spage><epage>141.e5</epage><pages>141.e1-141.e5</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><abstract>Objective To determine whether proangiogenic immature myeloid cells are present in human placentas. Study Design Biopsies were obtained from 61 placentas of term pregnancies. Percentage of CD45+ CD33+ LIN2− HLADR− immature myeloid cells of total CD45+ hematopoietic cells was determined by flow cytometry. Location of immature myeloid cells in the placenta was identified using confocal microscopy. The proangiogenic potential of immature myeloid cells was analyzed by endothelial tube formation. Results Immature myeloid cells comprise ∼25% of human placental CD45+ hematopoietic cells and infiltrate placentas in proximity of blood vessels. The percentage of immature myeloid cells correlated positively with placental weight (r 2 = 0.108, P = .01) and birthweight (r 2 = 0.087, P = .02). Endothelial tube formation was increased in the presence of immature myeloid cells as compared with the presence of CD45+ LIN2+ control cells. Conclusion Human placentas are populated by immature myeloid cells in the proximity of blood vessels. Consistent with their involvement in angiogenesis, immature myeloid cells accelerated endothelial tube formation. The presence of immature myeloid cells in pathologic pregnancies warrants further studies.</abstract><cop>United States</cop><pub>Mosby, Inc</pub><pmid>22840728</pmid><doi>10.1016/j.ajog.2012.05.025</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9378 |
| ispartof | American journal of obstetrics and gynecology, 2012-08, Vol.207 (2), p.141.e1-141.e5 |
| issn | 0002-9378 1097-6868 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1030350829 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adult angiogenesis Antigens, CD - metabolism Antigens, Differentiation, Myelomonocytic - metabolism Birth Weight Endothelium, Vascular - embryology Female Flow Cytometry Humans immature myeloid cells Infant, Newborn Leukocyte Common Antigens - metabolism Microscopy, Confocal Myeloid Cells - metabolism Myeloid Cells - physiology Neovascularization, Physiologic Obstetrics and Gynecology Organ Size placenta Placenta - anatomy & histology Placenta - cytology Pregnancy Sialic Acid Binding Ig-like Lectin 3 |
| title | Proangiogenic immature myeloid cells populate the human placenta and their presence correlates with placental and birthweight |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T19%3A46%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Proangiogenic%20immature%20myeloid%20cells%20populate%20the%20human%20placenta%20and%20their%20presence%20correlates%20with%20placental%20and%20birthweight&rft.jtitle=American%20journal%20of%20obstetrics%20and%20gynecology&rft.au=Mei-Dan,%20Elad,%20MD&rft.date=2012-08-01&rft.volume=207&rft.issue=2&rft.spage=141.e1&rft.epage=141.e5&rft.pages=141.e1-141.e5&rft.issn=0002-9378&rft.eissn=1097-6868&rft_id=info:doi/10.1016/j.ajog.2012.05.025&rft_dat=%3Cproquest_cross%3E1030350829%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1030350829&rft_id=info:pmid/22840728&rft_els_id=S0002937812005881&rfr_iscdi=true |