Conducting polyamic acid membranes for sensing and site-directed immobilization of proteins

A biosensor platform based on polyamic acid (PAA) is reported for oriented immobilization of biomolecules. PAA, a functionalized conducting polymer substrate that provides electrochemical detection and control of biospecific binding, was used to covalently attach biomolecules, resulting in a signifi...

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Veröffentlicht in:	Analytical biochemistry 2012-09, Vol.428 (1), p.54-63
Hauptverfasser:	Noah, Naumih M., Omole, Marcells, Stern, Samantha, Zhang, Siyi, Sadik, Omowunmi A., Hess, Euodia H., Martinovic, Jasmina, Baker, Priscilla G.L., Iwuoha, Emmanuel I.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amperometry Animals antibodies antigens Benzene Derivatives - chemistry Biosensing Techniques - methods biosensors detection limit dielectric spectroscopy Diffusion diffusivity Electric Conductivity Electrochemical Techniques electrochemistry Electrodes electron transfer Enzyme-Linked Immunosorbent Assay Fourier transform infrared spectroscopy gold Immobilized Proteins - metabolism immunoglobulin G inducible nitric oxide synthase Inducible nitric oxide synthase (iNOS) Magnetic Resonance Spectroscopy Membranes, Artificial Mice Mouse IgG Nitric Oxide Synthase Type II - metabolism nuclear magnetic resonance spectroscopy Polyamic acid (PAA) polymers Polymers - chemistry Sensor template Signal amplification Spectroscopy, Fourier Transform Infrared Surface Plasmon Resonance Surface plasmon resonance (SPR) Time Factors
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