Field and laboratory studies on the bioconversion of coal to methane in the San Juan Basin
Abstract The bioconversion of coal to methane in the San Juan Basin, New Mexico, was investigated. Production waters were analyzed via enrichment studies, metabolite-profiling, and culture-independent methods. Analysis of 16S rRNA gene sequences indicated the presence of methanogens potentially capa...
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| Veröffentlicht in: | FEMS microbiology ecology 2012-07, Vol.81 (1), p.26-42 |
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| creator | Wawrik, Boris Mendivelso, Margarita Parisi, Victoria A. Suflita, Joseph M. Davidova, Irene A. Marks, Christopher R. Van Nostrand, Joy D. Liang, Yuting Zhou, Jizhong Huizinga, Brad J. Strąpoć, Dariusz Callaghan, Amy V. |
| description | Abstract
The bioconversion of coal to methane in the San Juan Basin, New Mexico, was investigated. Production waters were analyzed via enrichment studies, metabolite-profiling, and culture-independent methods. Analysis of 16S rRNA gene sequences indicated the presence of methanogens potentially capable of acetoclastic, hydrogenotrophic, and methylotrophic metabolisms, predominantly belonging to the Methanosarcinales and Methanomicrobiales. Incubations of produced water and coal readily produced methane, but there was no correlation between the thermal maturity and methanogenesis. Coal methanogenesis was greater when samples with a greater richness of Firmicutes were utilized. A greater archaeal diversity was observed in the presence of several aromatic and short-chain fatty acid metabolites. Incubations amended with lactate, hydrogen, formate, and short-chain alcohols produced methane above un-amended controls. Methanogenesis from acetate was not observed. Metabolite profiling showed the widespread occurrence of putative aromatic ring intermediates including benzoate, toluic acids, phthalic acids, and cresols. The detection of saturated and unsaturated alkylsuccinic acids indicated n-alkane and cyclic alkane/alkene metabolism. Microarray analysis complemented observations based on hybridization to functional genes related to the anaerobic metabolism of aromatic and aliphatic substrates. These data suggest that coal methanogenesis is unlikely to be limited by methanogen biomass, but rather the activation and degradation of coal constituents. |
| doi_str_mv | 10.1111/j.1574-6941.2011.01272.x |
| format | Article |
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The bioconversion of coal to methane in the San Juan Basin, New Mexico, was investigated. Production waters were analyzed via enrichment studies, metabolite-profiling, and culture-independent methods. Analysis of 16S rRNA gene sequences indicated the presence of methanogens potentially capable of acetoclastic, hydrogenotrophic, and methylotrophic metabolisms, predominantly belonging to the Methanosarcinales and Methanomicrobiales. Incubations of produced water and coal readily produced methane, but there was no correlation between the thermal maturity and methanogenesis. Coal methanogenesis was greater when samples with a greater richness of Firmicutes were utilized. A greater archaeal diversity was observed in the presence of several aromatic and short-chain fatty acid metabolites. Incubations amended with lactate, hydrogen, formate, and short-chain alcohols produced methane above un-amended controls. Methanogenesis from acetate was not observed. Metabolite profiling showed the widespread occurrence of putative aromatic ring intermediates including benzoate, toluic acids, phthalic acids, and cresols. The detection of saturated and unsaturated alkylsuccinic acids indicated n-alkane and cyclic alkane/alkene metabolism. Microarray analysis complemented observations based on hybridization to functional genes related to the anaerobic metabolism of aromatic and aliphatic substrates. These data suggest that coal methanogenesis is unlikely to be limited by methanogen biomass, but rather the activation and degradation of coal constituents.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2011.01272.x</identifier><identifier>PMID: 22146015</identifier><identifier>CODEN: FMECEZ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Archaea - classification ; Archaea - genetics ; Archaea - metabolism ; Bacteria - classification ; Bacteria - genetics ; Bacteria - metabolism ; Base Sequence ; Coal ; coal‐bed methane ; Ecology ; Fatty acids ; Firmicutes ; Genes ; Hydrogen - metabolism ; metabolite analysis ; Metabolites ; Methane ; Methane - metabolism ; Methanogenesis ; Methanomicrobiales ; Methanomicrobiales - classification ; Methanomicrobiales - genetics ; Methanomicrobiales - metabolism ; Methanosarcinales ; Methanosarcinales - classification ; Methanosarcinales - genetics ; Methanosarcinales - metabolism ; Microbiology ; Molecular Sequence Data ; New Mexico ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; subsurface microbiology</subject><ispartof>FEMS microbiology ecology, 2012-07, Vol.81 (1), p.26-42</ispartof><rights>2012 Federation of European Microbiological Societies 2012</rights><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</rights><rights>Copyright © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5902-2aa072b7c0ef063a44c20caa6ea305028e7a4ef95ecc0ec2c8536ada5787eecd3</citedby><cites>FETCH-LOGICAL-c5902-2aa072b7c0ef063a44c20caa6ea305028e7a4ef95ecc0ec2c8536ada5787eecd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6941.2011.01272.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6941.2011.01272.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22146015$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wawrik, Boris</creatorcontrib><creatorcontrib>Mendivelso, Margarita</creatorcontrib><creatorcontrib>Parisi, Victoria A.</creatorcontrib><creatorcontrib>Suflita, Joseph M.</creatorcontrib><creatorcontrib>Davidova, Irene A.</creatorcontrib><creatorcontrib>Marks, Christopher R.</creatorcontrib><creatorcontrib>Van Nostrand, Joy D.</creatorcontrib><creatorcontrib>Liang, Yuting</creatorcontrib><creatorcontrib>Zhou, Jizhong</creatorcontrib><creatorcontrib>Huizinga, Brad J.</creatorcontrib><creatorcontrib>Strąpoć, Dariusz</creatorcontrib><creatorcontrib>Callaghan, Amy V.</creatorcontrib><title>Field and laboratory studies on the bioconversion of coal to methane in the San Juan Basin</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Abstract
The bioconversion of coal to methane in the San Juan Basin, New Mexico, was investigated. Production waters were analyzed via enrichment studies, metabolite-profiling, and culture-independent methods. Analysis of 16S rRNA gene sequences indicated the presence of methanogens potentially capable of acetoclastic, hydrogenotrophic, and methylotrophic metabolisms, predominantly belonging to the Methanosarcinales and Methanomicrobiales. Incubations of produced water and coal readily produced methane, but there was no correlation between the thermal maturity and methanogenesis. Coal methanogenesis was greater when samples with a greater richness of Firmicutes were utilized. A greater archaeal diversity was observed in the presence of several aromatic and short-chain fatty acid metabolites. Incubations amended with lactate, hydrogen, formate, and short-chain alcohols produced methane above un-amended controls. Methanogenesis from acetate was not observed. Metabolite profiling showed the widespread occurrence of putative aromatic ring intermediates including benzoate, toluic acids, phthalic acids, and cresols. The detection of saturated and unsaturated alkylsuccinic acids indicated n-alkane and cyclic alkane/alkene metabolism. Microarray analysis complemented observations based on hybridization to functional genes related to the anaerobic metabolism of aromatic and aliphatic substrates. These data suggest that coal methanogenesis is unlikely to be limited by methanogen biomass, but rather the activation and degradation of coal constituents.</description><subject>Archaea - classification</subject><subject>Archaea - genetics</subject><subject>Archaea - metabolism</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - metabolism</subject><subject>Base Sequence</subject><subject>Coal</subject><subject>coal‐bed methane</subject><subject>Ecology</subject><subject>Fatty acids</subject><subject>Firmicutes</subject><subject>Genes</subject><subject>Hydrogen - metabolism</subject><subject>metabolite analysis</subject><subject>Metabolites</subject><subject>Methane</subject><subject>Methane - metabolism</subject><subject>Methanogenesis</subject><subject>Methanomicrobiales</subject><subject>Methanomicrobiales - classification</subject><subject>Methanomicrobiales - genetics</subject><subject>Methanomicrobiales - metabolism</subject><subject>Methanosarcinales</subject><subject>Methanosarcinales - classification</subject><subject>Methanosarcinales - genetics</subject><subject>Methanosarcinales - metabolism</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>New Mexico</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>subsurface microbiology</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v1DAQxS0EokvhKyBLXLgkjP_EyR44QNWlRUUcgAsXa9aZqF5l48VOSvfb45DSAwipPthj-_eex3qMcQGlyOPNrhRVrQuz1qKUIEQJQtayvH3EVvcXj9kKhGkKo9fmhD1LaQcgKqXhKTuRUmiTdyv2feOpbzkOLe9xGyKOIR55GqfWU-Jh4OM18a0PLgw3FJPPJ6HjLmDPx8D3NF7jQNwv3Bcc-McpT-8x-eE5e9Jhn-jF3XrKvm3Ov55dFFefP1yevbsqXLUGWUhEqOW2dkAdGIVaOwkO0RAqqEA2VKOmbl2Ry4iTrqmUwRaruqmJXKtO2evF9xDDj4nSaPc-Oer73FmYkhXZA7JV1j0AhUaBaHRGX_2F7sIUh_wRKypRa2mkUplqFsrFkFKkzh6i32M8Zis7R2V3dk7EzonYOSr7Oyp7m6Uv7x6Ytntq74V_ssnA2wX46Xs6PtjYbs4_zVXWq0UfpsN_1MW_bf0C5nWviw</recordid><startdate>201207</startdate><enddate>201207</enddate><creator>Wawrik, Boris</creator><creator>Mendivelso, Margarita</creator><creator>Parisi, Victoria A.</creator><creator>Suflita, Joseph M.</creator><creator>Davidova, Irene A.</creator><creator>Marks, Christopher R.</creator><creator>Van Nostrand, Joy D.</creator><creator>Liang, Yuting</creator><creator>Zhou, Jizhong</creator><creator>Huizinga, Brad J.</creator><creator>Strąpoć, Dariusz</creator><creator>Callaghan, Amy V.</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7QH</scope><scope>7UA</scope><scope>F1W</scope><scope>H96</scope><scope>L.G</scope></search><sort><creationdate>201207</creationdate><title>Field and laboratory studies on the bioconversion of coal to methane in the San Juan Basin</title><author>Wawrik, Boris ; 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The bioconversion of coal to methane in the San Juan Basin, New Mexico, was investigated. Production waters were analyzed via enrichment studies, metabolite-profiling, and culture-independent methods. Analysis of 16S rRNA gene sequences indicated the presence of methanogens potentially capable of acetoclastic, hydrogenotrophic, and methylotrophic metabolisms, predominantly belonging to the Methanosarcinales and Methanomicrobiales. Incubations of produced water and coal readily produced methane, but there was no correlation between the thermal maturity and methanogenesis. Coal methanogenesis was greater when samples with a greater richness of Firmicutes were utilized. A greater archaeal diversity was observed in the presence of several aromatic and short-chain fatty acid metabolites. Incubations amended with lactate, hydrogen, formate, and short-chain alcohols produced methane above un-amended controls. Methanogenesis from acetate was not observed. Metabolite profiling showed the widespread occurrence of putative aromatic ring intermediates including benzoate, toluic acids, phthalic acids, and cresols. The detection of saturated and unsaturated alkylsuccinic acids indicated n-alkane and cyclic alkane/alkene metabolism. Microarray analysis complemented observations based on hybridization to functional genes related to the anaerobic metabolism of aromatic and aliphatic substrates. These data suggest that coal methanogenesis is unlikely to be limited by methanogen biomass, but rather the activation and degradation of coal constituents.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22146015</pmid><doi>10.1111/j.1574-6941.2011.01272.x</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Archaea - classification Archaea - genetics Archaea - metabolism Bacteria - classification Bacteria - genetics Bacteria - metabolism Base Sequence Coal coal‐bed methane Ecology Fatty acids Firmicutes Genes Hydrogen - metabolism metabolite analysis Metabolites Methane Methane - metabolism Methanogenesis Methanomicrobiales Methanomicrobiales - classification Methanomicrobiales - genetics Methanomicrobiales - metabolism Methanosarcinales Methanosarcinales - classification Methanosarcinales - genetics Methanosarcinales - metabolism Microbiology Molecular Sequence Data New Mexico Phylogeny RNA, Ribosomal, 16S - genetics subsurface microbiology |
| title | Field and laboratory studies on the bioconversion of coal to methane in the San Juan Basin |
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