BDE-47 disrupts axonal growth and motor behavior in developing zebrafish
► BDE-47 exposure significantly affected spontaneous movement, decreased touch response and free swimming speed, altered larvae swimming behavior in response to light stimulation in developing zebrafish. ► Consistent with motor deficits, BDE-47 significantly inhibited axonal growth of primary and se...
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| Veröffentlicht in: | Aquatic toxicology 2012-09, Vol.120-121, p.35-44 |
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| creator | Chen, Xiaojuan Huang, Changjiang Wang, Xuechun Chen, Jiangfei Bai, Chenglian Chen, Yuanhong Chen, Xiangping Dong, Qiaoxiang Yang, Dongren |
| description | ► BDE-47 exposure significantly affected spontaneous movement, decreased touch response and free swimming speed, altered larvae swimming behavior in response to light stimulation in developing zebrafish. ► Consistent with motor deficits, BDE-47 significantly inhibited axonal growth of primary and secondary motor neurons during the early developmental stages, suggesting the functional relevance of structural changes. ► The altered patterns of neuronal connectivity may contribute to motor behavior deficits, indicating the relevance of zebrafish as a model for studying BDE-47 developmental neurotoxicity.
Polybrominated diphenyl ethers (PBDEs) are ubiquitous environmental pollutants detected in a wide variety of environmental matrixes and pose a significant public health concern. 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47) is one of the most predominant PBDE congeners in environmental media, biota and human tissues. However, few studies have explored the BDE-47 developmental neurotoxicity and underlying mechanisms. In this study, zebrafish (Danio rerio) embryos were waterborne exposed to BDE-47 at 1.25, 5, 20μM starting from 6h post-fertilization (hpf). Motor behavior development and swimming behavior in response to light-to-dark photoperiod stimulation were studied at various developmental stages. Our data indicate that BDE-47 exposure significantly affected spontaneous movement, decreased touch response and free swimming speed, altered larvae swimming behavior in response to light stimulation in developing zebrafish. Consistent with these motor deficits, BDE-47 significantly inhibited axonal growth of primary and secondary motor neurons during the early developmental stages, suggesting the functional relevance of structural changes. Our findings demonstrate that the altered patterns of neuronal connectivity may contribute to motor behavior deficits, indicating the relevance of zebrafish as a model for studying toxicant developmental neurotoxicity. |
| doi_str_mv | 10.1016/j.aquatox.2012.04.014 |
| format | Article |
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Polybrominated diphenyl ethers (PBDEs) are ubiquitous environmental pollutants detected in a wide variety of environmental matrixes and pose a significant public health concern. 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47) is one of the most predominant PBDE congeners in environmental media, biota and human tissues. However, few studies have explored the BDE-47 developmental neurotoxicity and underlying mechanisms. In this study, zebrafish (Danio rerio) embryos were waterborne exposed to BDE-47 at 1.25, 5, 20μM starting from 6h post-fertilization (hpf). Motor behavior development and swimming behavior in response to light-to-dark photoperiod stimulation were studied at various developmental stages. Our data indicate that BDE-47 exposure significantly affected spontaneous movement, decreased touch response and free swimming speed, altered larvae swimming behavior in response to light stimulation in developing zebrafish. Consistent with these motor deficits, BDE-47 significantly inhibited axonal growth of primary and secondary motor neurons during the early developmental stages, suggesting the functional relevance of structural changes. Our findings demonstrate that the altered patterns of neuronal connectivity may contribute to motor behavior deficits, indicating the relevance of zebrafish as a model for studying toxicant developmental neurotoxicity.</description><identifier>ISSN: 0166-445X</identifier><identifier>EISSN: 1879-1514</identifier><identifier>DOI: 10.1016/j.aquatox.2012.04.014</identifier><identifier>PMID: 22609740</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Axonal growth ; Axons - drug effects ; Axons - physiology ; BDE-47 ; Danio rerio ; early development ; Embryo, Nonmammalian - drug effects ; Embryo, Nonmammalian - embryology ; Embryo, Nonmammalian - physiology ; Flame Retardants - toxicity ; Freshwater ; Gas Chromatography-Mass Spectrometry ; Halogenated Diphenyl Ethers ; Immunohistochemistry ; Larva - genetics ; Larva - growth & development ; Larva - metabolism ; Larva - physiology ; larvae ; Motor behavior ; motor neurons ; Motor Neurons - drug effects ; Motor Neurons - physiology ; Neurotoxicity ; pollutants ; Polybrominated Biphenyls - toxicity ; polybrominated diphenyl ethers ; public health ; Random Allocation ; Real-Time Polymerase Chain Reaction ; Swimming ; Water Pollutants, Chemical - toxicity ; Zebrafish ; Zebrafish - embryology ; Zebrafish - genetics ; Zebrafish - growth & development ; Zebrafish - physiology</subject><ispartof>Aquatic toxicology, 2012-09, Vol.120-121, p.35-44</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-d9a8e9f4be41f3e58a7b1019fb298cc7e1709ab0d948f16c39ee8f8722ce4c833</citedby><cites>FETCH-LOGICAL-c422t-d9a8e9f4be41f3e58a7b1019fb298cc7e1709ab0d948f16c39ee8f8722ce4c833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aquatox.2012.04.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22609740$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Xiaojuan</creatorcontrib><creatorcontrib>Huang, Changjiang</creatorcontrib><creatorcontrib>Wang, Xuechun</creatorcontrib><creatorcontrib>Chen, Jiangfei</creatorcontrib><creatorcontrib>Bai, Chenglian</creatorcontrib><creatorcontrib>Chen, Yuanhong</creatorcontrib><creatorcontrib>Chen, Xiangping</creatorcontrib><creatorcontrib>Dong, Qiaoxiang</creatorcontrib><creatorcontrib>Yang, Dongren</creatorcontrib><title>BDE-47 disrupts axonal growth and motor behavior in developing zebrafish</title><title>Aquatic toxicology</title><addtitle>Aquat Toxicol</addtitle><description>► BDE-47 exposure significantly affected spontaneous movement, decreased touch response and free swimming speed, altered larvae swimming behavior in response to light stimulation in developing zebrafish. ► Consistent with motor deficits, BDE-47 significantly inhibited axonal growth of primary and secondary motor neurons during the early developmental stages, suggesting the functional relevance of structural changes. ► The altered patterns of neuronal connectivity may contribute to motor behavior deficits, indicating the relevance of zebrafish as a model for studying BDE-47 developmental neurotoxicity.
Polybrominated diphenyl ethers (PBDEs) are ubiquitous environmental pollutants detected in a wide variety of environmental matrixes and pose a significant public health concern. 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47) is one of the most predominant PBDE congeners in environmental media, biota and human tissues. However, few studies have explored the BDE-47 developmental neurotoxicity and underlying mechanisms. In this study, zebrafish (Danio rerio) embryos were waterborne exposed to BDE-47 at 1.25, 5, 20μM starting from 6h post-fertilization (hpf). Motor behavior development and swimming behavior in response to light-to-dark photoperiod stimulation were studied at various developmental stages. Our data indicate that BDE-47 exposure significantly affected spontaneous movement, decreased touch response and free swimming speed, altered larvae swimming behavior in response to light stimulation in developing zebrafish. Consistent with these motor deficits, BDE-47 significantly inhibited axonal growth of primary and secondary motor neurons during the early developmental stages, suggesting the functional relevance of structural changes. Our findings demonstrate that the altered patterns of neuronal connectivity may contribute to motor behavior deficits, indicating the relevance of zebrafish as a model for studying toxicant developmental neurotoxicity.</description><subject>Animals</subject><subject>Axonal growth</subject><subject>Axons - drug effects</subject><subject>Axons - physiology</subject><subject>BDE-47</subject><subject>Danio rerio</subject><subject>early development</subject><subject>Embryo, Nonmammalian - drug effects</subject><subject>Embryo, Nonmammalian - embryology</subject><subject>Embryo, Nonmammalian - physiology</subject><subject>Flame Retardants - toxicity</subject><subject>Freshwater</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Halogenated Diphenyl Ethers</subject><subject>Immunohistochemistry</subject><subject>Larva - genetics</subject><subject>Larva - growth & development</subject><subject>Larva - metabolism</subject><subject>Larva - physiology</subject><subject>larvae</subject><subject>Motor behavior</subject><subject>motor neurons</subject><subject>Motor Neurons - drug effects</subject><subject>Motor Neurons - physiology</subject><subject>Neurotoxicity</subject><subject>pollutants</subject><subject>Polybrominated Biphenyls - toxicity</subject><subject>polybrominated diphenyl ethers</subject><subject>public health</subject><subject>Random Allocation</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Swimming</subject><subject>Water Pollutants, Chemical - toxicity</subject><subject>Zebrafish</subject><subject>Zebrafish - embryology</subject><subject>Zebrafish - genetics</subject><subject>Zebrafish - growth & development</subject><subject>Zebrafish - physiology</subject><issn>0166-445X</issn><issn>1879-1514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtO6zAQQC0EgvL4BCBLNgm249T2CkGBCxISC0BiZznOuHWVxsVOCvd-Pa7ay5bZzCzOvA5CpwQXBJPx5bzQH4Pu_VdBMaEFZgUmbAeNiOAyJxVhu2iUuHHOWPV-gA5jnOMUlMl9dEDpGEvO8Ag93Nze5YxnjYthWPYx01--0202Df6zn2W6a7KF733IapjplUuF67IGVtD6peum2T-og7Yuzo7RntVthJNtPkJv93evk4f86fnP4-T6KTeM0j5vpBYgLauBEVtCJTSv0z_S1lQKYzgQjqWucSOZsGRsSgkgrOCUGmBGlOURutjMXQb_MUDs1cJFA22rO_BDVARTgSknnCa02qAm-BgDWLUMbqHD3wSptUQ1V1uJai1RYaaSxNR3tl0x1Atofrr-W0vA-Qaw2is9DS6qt5c0gWFMKs7L9ZVXGwKSipWDoKJx0BloXADTq8a7X474Bh2HjtY</recordid><startdate>20120915</startdate><enddate>20120915</enddate><creator>Chen, Xiaojuan</creator><creator>Huang, Changjiang</creator><creator>Wang, Xuechun</creator><creator>Chen, Jiangfei</creator><creator>Bai, Chenglian</creator><creator>Chen, Yuanhong</creator><creator>Chen, Xiangping</creator><creator>Dong, Qiaoxiang</creator><creator>Yang, Dongren</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>SOI</scope></search><sort><creationdate>20120915</creationdate><title>BDE-47 disrupts axonal growth and motor behavior in developing zebrafish</title><author>Chen, Xiaojuan ; Huang, Changjiang ; Wang, Xuechun ; Chen, Jiangfei ; Bai, Chenglian ; Chen, Yuanhong ; Chen, Xiangping ; Dong, Qiaoxiang ; Yang, Dongren</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-d9a8e9f4be41f3e58a7b1019fb298cc7e1709ab0d948f16c39ee8f8722ce4c833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Axonal growth</topic><topic>Axons - drug effects</topic><topic>Axons - physiology</topic><topic>BDE-47</topic><topic>Danio rerio</topic><topic>early development</topic><topic>Embryo, Nonmammalian - drug effects</topic><topic>Embryo, Nonmammalian - embryology</topic><topic>Embryo, Nonmammalian - physiology</topic><topic>Flame Retardants - toxicity</topic><topic>Freshwater</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Halogenated Diphenyl Ethers</topic><topic>Immunohistochemistry</topic><topic>Larva - genetics</topic><topic>Larva - growth & development</topic><topic>Larva - metabolism</topic><topic>Larva - physiology</topic><topic>larvae</topic><topic>Motor behavior</topic><topic>motor neurons</topic><topic>Motor Neurons - drug effects</topic><topic>Motor Neurons - physiology</topic><topic>Neurotoxicity</topic><topic>pollutants</topic><topic>Polybrominated Biphenyls - toxicity</topic><topic>polybrominated diphenyl ethers</topic><topic>public health</topic><topic>Random Allocation</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Swimming</topic><topic>Water Pollutants, Chemical - toxicity</topic><topic>Zebrafish</topic><topic>Zebrafish - embryology</topic><topic>Zebrafish - genetics</topic><topic>Zebrafish - growth & development</topic><topic>Zebrafish - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Xiaojuan</creatorcontrib><creatorcontrib>Huang, Changjiang</creatorcontrib><creatorcontrib>Wang, Xuechun</creatorcontrib><creatorcontrib>Chen, Jiangfei</creatorcontrib><creatorcontrib>Bai, Chenglian</creatorcontrib><creatorcontrib>Chen, Yuanhong</creatorcontrib><creatorcontrib>Chen, Xiangping</creatorcontrib><creatorcontrib>Dong, Qiaoxiang</creatorcontrib><creatorcontrib>Yang, Dongren</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><jtitle>Aquatic toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Xiaojuan</au><au>Huang, Changjiang</au><au>Wang, Xuechun</au><au>Chen, Jiangfei</au><au>Bai, Chenglian</au><au>Chen, Yuanhong</au><au>Chen, Xiangping</au><au>Dong, Qiaoxiang</au><au>Yang, Dongren</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>BDE-47 disrupts axonal growth and motor behavior in developing zebrafish</atitle><jtitle>Aquatic toxicology</jtitle><addtitle>Aquat Toxicol</addtitle><date>2012-09-15</date><risdate>2012</risdate><volume>120-121</volume><spage>35</spage><epage>44</epage><pages>35-44</pages><issn>0166-445X</issn><eissn>1879-1514</eissn><abstract>► BDE-47 exposure significantly affected spontaneous movement, decreased touch response and free swimming speed, altered larvae swimming behavior in response to light stimulation in developing zebrafish. ► Consistent with motor deficits, BDE-47 significantly inhibited axonal growth of primary and secondary motor neurons during the early developmental stages, suggesting the functional relevance of structural changes. ► The altered patterns of neuronal connectivity may contribute to motor behavior deficits, indicating the relevance of zebrafish as a model for studying BDE-47 developmental neurotoxicity.
Polybrominated diphenyl ethers (PBDEs) are ubiquitous environmental pollutants detected in a wide variety of environmental matrixes and pose a significant public health concern. 2,2′,4,4′-Tetrabromodiphenyl ether (BDE-47) is one of the most predominant PBDE congeners in environmental media, biota and human tissues. However, few studies have explored the BDE-47 developmental neurotoxicity and underlying mechanisms. In this study, zebrafish (Danio rerio) embryos were waterborne exposed to BDE-47 at 1.25, 5, 20μM starting from 6h post-fertilization (hpf). Motor behavior development and swimming behavior in response to light-to-dark photoperiod stimulation were studied at various developmental stages. Our data indicate that BDE-47 exposure significantly affected spontaneous movement, decreased touch response and free swimming speed, altered larvae swimming behavior in response to light stimulation in developing zebrafish. Consistent with these motor deficits, BDE-47 significantly inhibited axonal growth of primary and secondary motor neurons during the early developmental stages, suggesting the functional relevance of structural changes. Our findings demonstrate that the altered patterns of neuronal connectivity may contribute to motor behavior deficits, indicating the relevance of zebrafish as a model for studying toxicant developmental neurotoxicity.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22609740</pmid><doi>10.1016/j.aquatox.2012.04.014</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0166-445X |
| ispartof | Aquatic toxicology, 2012-09, Vol.120-121, p.35-44 |
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| subjects | Animals Axonal growth Axons - drug effects Axons - physiology BDE-47 Danio rerio early development Embryo, Nonmammalian - drug effects Embryo, Nonmammalian - embryology Embryo, Nonmammalian - physiology Flame Retardants - toxicity Freshwater Gas Chromatography-Mass Spectrometry Halogenated Diphenyl Ethers Immunohistochemistry Larva - genetics Larva - growth & development Larva - metabolism Larva - physiology larvae Motor behavior motor neurons Motor Neurons - drug effects Motor Neurons - physiology Neurotoxicity pollutants Polybrominated Biphenyls - toxicity polybrominated diphenyl ethers public health Random Allocation Real-Time Polymerase Chain Reaction Swimming Water Pollutants, Chemical - toxicity Zebrafish Zebrafish - embryology Zebrafish - genetics Zebrafish - growth & development Zebrafish - physiology |
| title | BDE-47 disrupts axonal growth and motor behavior in developing zebrafish |
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