The detection of HBsAg mutants expressed in vitro using two different quantitative HBsAg assays
Abstract Background The quantification of hepatitis-B surface antigen (HBsAg) is useful to identify inactive carriers in chronically HBV infected patients and to predict interferon treatment outcome. Objective To compare two quantitative HBsAg assays for the detection of HBsAg mutants expressed in v...
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| Veröffentlicht in: | Journal of clinical virology 2012-07, Vol.54 (3), p.279-281 |
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| creator | Verheyen, Jens Neumann-Fraune, Maria Berg, Thomas Kaiser, Rolf Obermeier, Martin |
| description | Abstract Background The quantification of hepatitis-B surface antigen (HBsAg) is useful to identify inactive carriers in chronically HBV infected patients and to predict interferon treatment outcome. Objective To compare two quantitative HBsAg assays for the detection of HBsAg mutants expressed in vitro. Study design HBsAg mutants ( n = 35) were expressed in HuH7 cells and the supernatants were tested in two different quantitative HBsAg assays (Architect, Abbott and Elecsys, Roche). Results All HBsAg mutants were detected by both assays, but in general the results of the Architect system were higher than those of the Elecsys system. The detection of HBsAg mutants in comparison to wild type was similar using both assays. However, HBsAg mutation T123A was under quantified by the Architect, whereas HBsAg mutations P142L, P142S and G145K yielded lower results in the Elecsys system. Conclusions Even though HBsAg assays are optimised for the detection of HBsAg mutants, discrepant results were obtained for some HBsAg mutants in two quantitative HBsAg assays. These findings have to be considered when testing samples from one patient with two different quantitative HBsAg assays. |
| doi_str_mv | 10.1016/j.jcv.2012.04.010 |
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Objective To compare two quantitative HBsAg assays for the detection of HBsAg mutants expressed in vitro. Study design HBsAg mutants ( n = 35) were expressed in HuH7 cells and the supernatants were tested in two different quantitative HBsAg assays (Architect, Abbott and Elecsys, Roche). Results All HBsAg mutants were detected by both assays, but in general the results of the Architect system were higher than those of the Elecsys system. The detection of HBsAg mutants in comparison to wild type was similar using both assays. However, HBsAg mutation T123A was under quantified by the Architect, whereas HBsAg mutations P142L, P142S and G145K yielded lower results in the Elecsys system. Conclusions Even though HBsAg assays are optimised for the detection of HBsAg mutants, discrepant results were obtained for some HBsAg mutants in two quantitative HBsAg assays. These findings have to be considered when testing samples from one patient with two different quantitative HBsAg assays.</description><identifier>ISSN: 1386-6532</identifier><identifier>EISSN: 1873-5967</identifier><identifier>DOI: 10.1016/j.jcv.2012.04.010</identifier><identifier>PMID: 22595307</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Allergy and Immunology ; Biological and medical sciences ; Cell Line ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; HBsAg ; Hepatitis B surface antigen ; Hepatitis B Surface Antigens - analysis ; Hepatitis B Surface Antigens - genetics ; Hepatitis B Surface Antigens - immunology ; Hepatitis B virus ; Hepatocytes - virology ; Human viral diseases ; Humans ; Infectious Disease ; Infectious diseases ; Interferon ; Medical sciences ; Microbiology ; Miscellaneous ; Mutant Proteins - analysis ; Mutant Proteins - genetics ; Mutant Proteins - immunology ; Mutants ; Mutation ; Quantitative assays ; Sensitivity and Specificity ; surface antigens ; Viral diseases ; Viral Load - methods ; Virology</subject><ispartof>Journal of clinical virology, 2012-07, Vol.54 (3), p.279-281</ispartof><rights>Elsevier B.V.</rights><rights>2012 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c537t-8e29f898f4e0431a70ec98d8caad5165b176deeca7d7b629723d563627fae1113</citedby><cites>FETCH-LOGICAL-c537t-8e29f898f4e0431a70ec98d8caad5165b176deeca7d7b629723d563627fae1113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S138665321200159X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25987002$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22595307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Verheyen, Jens</creatorcontrib><creatorcontrib>Neumann-Fraune, Maria</creatorcontrib><creatorcontrib>Berg, Thomas</creatorcontrib><creatorcontrib>Kaiser, Rolf</creatorcontrib><creatorcontrib>Obermeier, Martin</creatorcontrib><title>The detection of HBsAg mutants expressed in vitro using two different quantitative HBsAg assays</title><title>Journal of clinical virology</title><addtitle>J Clin Virol</addtitle><description>Abstract Background The quantification of hepatitis-B surface antigen (HBsAg) is useful to identify inactive carriers in chronically HBV infected patients and to predict interferon treatment outcome. Objective To compare two quantitative HBsAg assays for the detection of HBsAg mutants expressed in vitro. Study design HBsAg mutants ( n = 35) were expressed in HuH7 cells and the supernatants were tested in two different quantitative HBsAg assays (Architect, Abbott and Elecsys, Roche). Results All HBsAg mutants were detected by both assays, but in general the results of the Architect system were higher than those of the Elecsys system. The detection of HBsAg mutants in comparison to wild type was similar using both assays. However, HBsAg mutation T123A was under quantified by the Architect, whereas HBsAg mutations P142L, P142S and G145K yielded lower results in the Elecsys system. Conclusions Even though HBsAg assays are optimised for the detection of HBsAg mutants, discrepant results were obtained for some HBsAg mutants in two quantitative HBsAg assays. These findings have to be considered when testing samples from one patient with two different quantitative HBsAg assays.</description><subject>Allergy and Immunology</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>HBsAg</subject><subject>Hepatitis B surface antigen</subject><subject>Hepatitis B Surface Antigens - analysis</subject><subject>Hepatitis B Surface Antigens - genetics</subject><subject>Hepatitis B Surface Antigens - immunology</subject><subject>Hepatitis B virus</subject><subject>Hepatocytes - virology</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Interferon</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mutant Proteins - analysis</subject><subject>Mutant Proteins - genetics</subject><subject>Mutant Proteins - immunology</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Quantitative assays</subject><subject>Sensitivity and Specificity</subject><subject>surface antigens</subject><subject>Viral diseases</subject><subject>Viral Load - methods</subject><subject>Virology</subject><issn>1386-6532</issn><issn>1873-5967</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkkuLFDEUhQtRnIf-ADeSjeCmyjwqj0IQxmF0hAEXjuAupJNbY9rqqp7cVGv_e1N0q-BCXOUuvnNuuOdU1TNGG0aZerVu1n7XcMp4Q9uGMvqgOmVGi1p2Sj8sszCqVlLwk-oMcU0pk6LVj6sTzmUnBdWnlb39CiRABp_jNJKpJ9dv8eKObObsxowEfmwTIEIgcSS7mNNEZozjHcnfJxJi30OCMZP7udAxuxx3cHRwiG6PT6pHvRsQnh7f8-rzu6vby-v65uP7D5cXN7WXQufaAO9605m-BdoK5jQF35lgvHNBMiVXTKsA4J0OeqV4p7kIUgnFde-AMSbOq5cH322a7mfAbDcRPQyDG2Ga0TLKDeVS_BfKjBKy5W1B2QH1aUJM0NttihuX9gVaOGXXtkRglwgsbW2JoGieH-3n1QbCb8WvmxfgxRFw6N3QJzf6iH842RlNKS_c6wMH5W67CMmijzB6CDGVuGyY4j-_8eYvtR_iGMvCb7AHXE9zGksgllksGvtp6cpSFcaXmnRfxE8dO7gv</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>Verheyen, Jens</creator><creator>Neumann-Fraune, Maria</creator><creator>Berg, Thomas</creator><creator>Kaiser, Rolf</creator><creator>Obermeier, Martin</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20120701</creationdate><title>The detection of HBsAg mutants expressed in vitro using two different quantitative HBsAg assays</title><author>Verheyen, Jens ; Neumann-Fraune, Maria ; Berg, Thomas ; Kaiser, Rolf ; Obermeier, Martin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c537t-8e29f898f4e0431a70ec98d8caad5165b176deeca7d7b629723d563627fae1113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Allergy and Immunology</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>HBsAg</topic><topic>Hepatitis B surface antigen</topic><topic>Hepatitis B Surface Antigens - analysis</topic><topic>Hepatitis B Surface Antigens - genetics</topic><topic>Hepatitis B Surface Antigens - immunology</topic><topic>Hepatitis B virus</topic><topic>Hepatocytes - virology</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Interferon</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mutant Proteins - analysis</topic><topic>Mutant Proteins - genetics</topic><topic>Mutant Proteins - immunology</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Quantitative assays</topic><topic>Sensitivity and Specificity</topic><topic>surface antigens</topic><topic>Viral diseases</topic><topic>Viral Load - methods</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verheyen, Jens</creatorcontrib><creatorcontrib>Neumann-Fraune, Maria</creatorcontrib><creatorcontrib>Berg, Thomas</creatorcontrib><creatorcontrib>Kaiser, Rolf</creatorcontrib><creatorcontrib>Obermeier, Martin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of clinical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verheyen, Jens</au><au>Neumann-Fraune, Maria</au><au>Berg, Thomas</au><au>Kaiser, Rolf</au><au>Obermeier, Martin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The detection of HBsAg mutants expressed in vitro using two different quantitative HBsAg assays</atitle><jtitle>Journal of clinical virology</jtitle><addtitle>J Clin Virol</addtitle><date>2012-07-01</date><risdate>2012</risdate><volume>54</volume><issue>3</issue><spage>279</spage><epage>281</epage><pages>279-281</pages><issn>1386-6532</issn><eissn>1873-5967</eissn><abstract>Abstract Background The quantification of hepatitis-B surface antigen (HBsAg) is useful to identify inactive carriers in chronically HBV infected patients and to predict interferon treatment outcome. Objective To compare two quantitative HBsAg assays for the detection of HBsAg mutants expressed in vitro. Study design HBsAg mutants ( n = 35) were expressed in HuH7 cells and the supernatants were tested in two different quantitative HBsAg assays (Architect, Abbott and Elecsys, Roche). Results All HBsAg mutants were detected by both assays, but in general the results of the Architect system were higher than those of the Elecsys system. The detection of HBsAg mutants in comparison to wild type was similar using both assays. However, HBsAg mutation T123A was under quantified by the Architect, whereas HBsAg mutations P142L, P142S and G145K yielded lower results in the Elecsys system. Conclusions Even though HBsAg assays are optimised for the detection of HBsAg mutants, discrepant results were obtained for some HBsAg mutants in two quantitative HBsAg assays. These findings have to be considered when testing samples from one patient with two different quantitative HBsAg assays.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>22595307</pmid><doi>10.1016/j.jcv.2012.04.010</doi><tpages>3</tpages></addata></record> |
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| subjects | Allergy and Immunology Biological and medical sciences Cell Line Fundamental and applied biological sciences. Psychology Gene Expression HBsAg Hepatitis B surface antigen Hepatitis B Surface Antigens - analysis Hepatitis B Surface Antigens - genetics Hepatitis B Surface Antigens - immunology Hepatitis B virus Hepatocytes - virology Human viral diseases Humans Infectious Disease Infectious diseases Interferon Medical sciences Microbiology Miscellaneous Mutant Proteins - analysis Mutant Proteins - genetics Mutant Proteins - immunology Mutants Mutation Quantitative assays Sensitivity and Specificity surface antigens Viral diseases Viral Load - methods Virology |
| title | The detection of HBsAg mutants expressed in vitro using two different quantitative HBsAg assays |
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