Mushroom Polysaccharides and Lipids Synthesized in Liquid Agitated and Static Cultures. Part I: Screening Various Mushroom Species
The effect of four synthetic media containing glucose (initial concentration 30 g l −1 ) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2012-06, Vol.167 (3), p.536-551 |
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| creator | Diamantopoulou, Panagiota Papanikolaou, Seraphim Kapoti, Maria Komaitis, Michael Aggelis, George Philippoussis, Antonios |
| description | The effect of four synthetic media containing glucose (initial concentration 30 g l
−1
) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids produced was also performed. Agitation had a positive effect on biomass production, glucose consumption and lipid biosynthesis. Media that favoured the production of biomass were not suitable for EPS biosynthesis and vice versa. Biomass values varied from ∼1.0 g l
−1
(
Lentinula edodes
) to ∼19 g l
−1
(
Pleurotus ostreatus
), while the highest EPS quantity achieved ranged between 1.6 and 1.8 g l
−1
(for
Ganoderma lucidum
and
L. edodes
, respectively). Quantities of total cellular lipids varied between 2.5 and 18.5 %
w
/
w
, in dry mycelial mass for the fungi tested. Lipid in dry weight values were influenced by the medium composition. Cellular lipids presented noticeable quantities of poly-unsaturated fatty acids like linoleic acid. Compared to most of the mushrooms tested, lipids of
Volvariella volvacea
were more saturated. The ability of several mushroom species of our study to produce in notable quantities the above-mentioned added-value compounds renders these fungi worthy for further investigations. |
| doi_str_mv | 10.1007/s12010-012-9713-9 |
| format | Article |
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−1
) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids produced was also performed. Agitation had a positive effect on biomass production, glucose consumption and lipid biosynthesis. Media that favoured the production of biomass were not suitable for EPS biosynthesis and vice versa. Biomass values varied from ∼1.0 g l
−1
(
Lentinula edodes
) to ∼19 g l
−1
(
Pleurotus ostreatus
), while the highest EPS quantity achieved ranged between 1.6 and 1.8 g l
−1
(for
Ganoderma lucidum
and
L. edodes
, respectively). Quantities of total cellular lipids varied between 2.5 and 18.5 %
w
/
w
, in dry mycelial mass for the fungi tested. Lipid in dry weight values were influenced by the medium composition. Cellular lipids presented noticeable quantities of poly-unsaturated fatty acids like linoleic acid. Compared to most of the mushrooms tested, lipids of
Volvariella volvacea
were more saturated. The ability of several mushroom species of our study to produce in notable quantities the above-mentioned added-value compounds renders these fungi worthy for further investigations.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-012-9713-9</identifier><identifier>PMID: 22573010</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Agaricales - cytology ; Agaricales - enzymology ; Agaricales - growth & development ; Agaricales - metabolism ; Biochemistry ; Biological and medical sciences ; Biomass ; Biosynthesis ; Biotechnology ; Cell culture ; Chemistry ; Chemistry and Materials Science ; Culture Media - chemistry ; Culture Techniques - methods ; Fundamental and applied biological sciences. Psychology ; Ganoderma lucidum ; Glucose - metabolism ; Lentinula edodes ; Lipids ; Lipids - analysis ; Lipids - biosynthesis ; Liquids ; Mushrooms ; Mycelium - growth & development ; Pleurotus ostreatus ; Polysaccharides - biosynthesis ; Polyunsaturated fatty acids ; Saccharides ; Species Specificity ; Volvariella volvacea</subject><ispartof>Applied biochemistry and biotechnology, 2012-06, Vol.167 (3), p.536-551</ispartof><rights>Springer Science+Business Media, LLC 2012</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-82c9c1825573c791cf83534a6ebe371d4445c2f156d689610556ae04647c64a43</citedby><cites>FETCH-LOGICAL-c501t-82c9c1825573c791cf83534a6ebe371d4445c2f156d689610556ae04647c64a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-012-9713-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-012-9713-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26010960$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22573010$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Diamantopoulou, Panagiota</creatorcontrib><creatorcontrib>Papanikolaou, Seraphim</creatorcontrib><creatorcontrib>Kapoti, Maria</creatorcontrib><creatorcontrib>Komaitis, Michael</creatorcontrib><creatorcontrib>Aggelis, George</creatorcontrib><creatorcontrib>Philippoussis, Antonios</creatorcontrib><title>Mushroom Polysaccharides and Lipids Synthesized in Liquid Agitated and Static Cultures. Part I: Screening Various Mushroom Species</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>The effect of four synthetic media containing glucose (initial concentration 30 g l
−1
) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids produced was also performed. Agitation had a positive effect on biomass production, glucose consumption and lipid biosynthesis. Media that favoured the production of biomass were not suitable for EPS biosynthesis and vice versa. Biomass values varied from ∼1.0 g l
−1
(
Lentinula edodes
) to ∼19 g l
−1
(
Pleurotus ostreatus
), while the highest EPS quantity achieved ranged between 1.6 and 1.8 g l
−1
(for
Ganoderma lucidum
and
L. edodes
, respectively). Quantities of total cellular lipids varied between 2.5 and 18.5 %
w
/
w
, in dry mycelial mass for the fungi tested. Lipid in dry weight values were influenced by the medium composition. Cellular lipids presented noticeable quantities of poly-unsaturated fatty acids like linoleic acid. Compared to most of the mushrooms tested, lipids of
Volvariella volvacea
were more saturated. The ability of several mushroom species of our study to produce in notable quantities the above-mentioned added-value compounds renders these fungi worthy for further investigations.</description><subject>Agaricales - cytology</subject><subject>Agaricales - enzymology</subject><subject>Agaricales - growth & development</subject><subject>Agaricales - metabolism</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Culture Media - chemistry</subject><subject>Culture Techniques - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ganoderma lucidum</subject><subject>Glucose - metabolism</subject><subject>Lentinula edodes</subject><subject>Lipids</subject><subject>Lipids - analysis</subject><subject>Lipids - biosynthesis</subject><subject>Liquids</subject><subject>Mushrooms</subject><subject>Mycelium - growth & development</subject><subject>Pleurotus ostreatus</subject><subject>Polysaccharides - biosynthesis</subject><subject>Polyunsaturated fatty acids</subject><subject>Saccharides</subject><subject>Species Specificity</subject><subject>Volvariella volvacea</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqN0Vtr1EAUB_BBLHatfgBfZEAEX1LnnoxvZfFS2NLCqq9hOjnZnZJNtnOSh_XRT-5Zdq1FEHxKmPmdS_Jn7JUU51KI8j1KJaQohFSFL6Uu_BM2k9b6Qigvn7KZUKUulKr8KXuOeCcIVrZ8xk6VsqWm0hn7eTXhOg_Dht8M3Q5DjOuQUwPIQ9_wRdqmBvly149rwPQDGp56Or2fUsMvVmkMIx3t5ZJeU-TzqRunDHjOb0Ie-eUHvowZoE_9in-nxsOE_GHicgsxAb5gJ23oEF4en2fs26ePX-dfisX158v5xaKIVsixqFT0UVbK0uqx9DK2lbbaBAe3oEvZGGNsVK20rnGVd1JY6wII40wZnQlGn7F3h77bPNxPgGO9SRih60IPtFcthaqE0kLL_6CSJhj6_UTf_EXvhin39CGkdOWNVsKSkgcV84CYoa23OW1C3hGq91nWhyxriqjeZ1l7qnl97DzdbqB5qPgdHoG3RxAwhq7NoY8J_zhHyLu9UweHdNWvID9e8V_TfwGTYrSG</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Diamantopoulou, Panagiota</creator><creator>Papanikolaou, Seraphim</creator><creator>Kapoti, Maria</creator><creator>Komaitis, Michael</creator><creator>Aggelis, George</creator><creator>Philippoussis, Antonios</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7QO</scope><scope>M7N</scope></search><sort><creationdate>20120601</creationdate><title>Mushroom Polysaccharides and Lipids Synthesized in Liquid Agitated and Static Cultures. 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Psychology</topic><topic>Ganoderma lucidum</topic><topic>Glucose - metabolism</topic><topic>Lentinula edodes</topic><topic>Lipids</topic><topic>Lipids - analysis</topic><topic>Lipids - biosynthesis</topic><topic>Liquids</topic><topic>Mushrooms</topic><topic>Mycelium - growth & development</topic><topic>Pleurotus ostreatus</topic><topic>Polysaccharides - biosynthesis</topic><topic>Polyunsaturated fatty acids</topic><topic>Saccharides</topic><topic>Species Specificity</topic><topic>Volvariella volvacea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Diamantopoulou, Panagiota</creatorcontrib><creatorcontrib>Papanikolaou, Seraphim</creatorcontrib><creatorcontrib>Kapoti, Maria</creatorcontrib><creatorcontrib>Komaitis, Michael</creatorcontrib><creatorcontrib>Aggelis, George</creatorcontrib><creatorcontrib>Philippoussis, Antonios</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Diamantopoulou, Panagiota</au><au>Papanikolaou, Seraphim</au><au>Kapoti, Maria</au><au>Komaitis, Michael</au><au>Aggelis, George</au><au>Philippoussis, Antonios</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mushroom Polysaccharides and Lipids Synthesized in Liquid Agitated and Static Cultures. Part I: Screening Various Mushroom Species</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>167</volume><issue>3</issue><spage>536</spage><epage>551</epage><pages>536-551</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><coden>ABIBDL</coden><abstract>The effect of four synthetic media containing glucose (initial concentration 30 g l
−1
) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids produced was also performed. Agitation had a positive effect on biomass production, glucose consumption and lipid biosynthesis. Media that favoured the production of biomass were not suitable for EPS biosynthesis and vice versa. Biomass values varied from ∼1.0 g l
−1
(
Lentinula edodes
) to ∼19 g l
−1
(
Pleurotus ostreatus
), while the highest EPS quantity achieved ranged between 1.6 and 1.8 g l
−1
(for
Ganoderma lucidum
and
L. edodes
, respectively). Quantities of total cellular lipids varied between 2.5 and 18.5 %
w
/
w
, in dry mycelial mass for the fungi tested. Lipid in dry weight values were influenced by the medium composition. Cellular lipids presented noticeable quantities of poly-unsaturated fatty acids like linoleic acid. Compared to most of the mushrooms tested, lipids of
Volvariella volvacea
were more saturated. The ability of several mushroom species of our study to produce in notable quantities the above-mentioned added-value compounds renders these fungi worthy for further investigations.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>22573010</pmid><doi>10.1007/s12010-012-9713-9</doi><tpages>16</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0273-2289 |
| ispartof | Applied biochemistry and biotechnology, 2012-06, Vol.167 (3), p.536-551 |
| issn | 0273-2289 1559-0291 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1028023031 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Agaricales - cytology Agaricales - enzymology Agaricales - growth & development Agaricales - metabolism Biochemistry Biological and medical sciences Biomass Biosynthesis Biotechnology Cell culture Chemistry Chemistry and Materials Science Culture Media - chemistry Culture Techniques - methods Fundamental and applied biological sciences. Psychology Ganoderma lucidum Glucose - metabolism Lentinula edodes Lipids Lipids - analysis Lipids - biosynthesis Liquids Mushrooms Mycelium - growth & development Pleurotus ostreatus Polysaccharides - biosynthesis Polyunsaturated fatty acids Saccharides Species Specificity Volvariella volvacea |
| title | Mushroom Polysaccharides and Lipids Synthesized in Liquid Agitated and Static Cultures. Part I: Screening Various Mushroom Species |
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