A rapid and robust assay for the determination of the amino acid hypusine as a possible biomarker for a high-throughput screening of antimalarials and for the diagnosis and therapy of different diseases

Eukaryotic initiation factor 5A (eIF5A) has recently been identified as a biomarker of prognostic significance and therapeutic potential for the treatment in hepatocellular carcinoma. This prompted us to establish a rapid and robust assay to determine deoxyhypusine and hypusine formed with the purif...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Amino acids 2012-05, Vol.42 (5), p.1651-1659
Hauptverfasser:	Kaiser, Annette, Khomutov, Alex R., Simonian, Alina, Agostinelli, Enzo
Format:	Artikel
Sprache:	eng
Schlagworte:	Aldehydes Amino acids Analytical Chemistry Animals Antimalarials - chemistry Antimalarials - isolation & purification Antimalarials - therapeutic use Assaying Biochemical Engineering Biochemistry Biomedical and Life Sciences Catalytic Domain Chromatography, High Pressure Liquid Drugs Enzymes Eukaryotic Initiation Factor-5 - genetics Eukaryotic Initiation Factor-5 - isolation & purification Eukaryotic Initiation Factor-5 - metabolism Humans Iron Life Sciences Lysine - analogs & derivatives Lysine - isolation & purification Lysine - metabolism Malaria - diagnosis Malaria - drug therapy Mathematical models Mice Mixed Function Oxygenases - isolation & purification Mixed Function Oxygenases - metabolism Neurobiology Original Article Oxidoreductases Acting on CH-NH Group Donors - isolation & purification Oxidoreductases Acting on CH-NH Group Donors - metabolism Plasmodium - enzymology Protein Hydrolysates - chemistry Proteomics Screening
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!