Molecular diagnosis of mango malformation disease and phylogeny of Fusarium mangiferae
Mango malformation disease (MMD) caused by various Fusaria, including the fungus Fusarium mangiferae , is difficult to diagnose and cannot be controlled effectively. In this study a PCR diagnostic tool was developed for detection of F. mangiferae by generating primers which flank fungus-specific seq...
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| Veröffentlicht in: | Phytoparasitica 2012-07, Vol.40 (3), p.287-297 |
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| creator | Newman, Zvi Freeman, Stanley Biton, Iris Sa’ada, David Paz, Tali Maymon, Marcel Lavi, Uri |
| description | Mango malformation disease (MMD) caused by various Fusaria, including the fungus
Fusarium mangiferae
, is difficult to diagnose and cannot be controlled effectively. In this study a PCR diagnostic tool was developed for detection of
F. mangiferae
by generating primers which flank fungus-specific sequences. The assay is suitable for both
in-vitro
and
in-vivo
tests, and is capable of detecting 10 pg of the fungal DNA. Relatively high genetic variation was discovered between various isolates of the pathogen based on amplified fragment length polymorphism (AFLP) analyses. In addition, some isolates of
F. mangiferae
(including the accession numbers FMS-123 and NRRL 25226), were found to be quite distinct from most
F. mangiferae
accessions. Likewise, representative MMD-associated Fusaria from the Asian and American clades of the
Gibberella fujikuroi
species complex were further differentiated by AFLP analyses. |
| doi_str_mv | 10.1007/s12600-012-0224-6 |
| format | Article |
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Fusarium mangiferae
, is difficult to diagnose and cannot be controlled effectively. In this study a PCR diagnostic tool was developed for detection of
F. mangiferae
by generating primers which flank fungus-specific sequences. The assay is suitable for both
in-vitro
and
in-vivo
tests, and is capable of detecting 10 pg of the fungal DNA. Relatively high genetic variation was discovered between various isolates of the pathogen based on amplified fragment length polymorphism (AFLP) analyses. In addition, some isolates of
F. mangiferae
(including the accession numbers FMS-123 and NRRL 25226), were found to be quite distinct from most
F. mangiferae
accessions. Likewise, representative MMD-associated Fusaria from the Asian and American clades of the
Gibberella fujikuroi
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Fusarium mangiferae
, is difficult to diagnose and cannot be controlled effectively. In this study a PCR diagnostic tool was developed for detection of
F. mangiferae
by generating primers which flank fungus-specific sequences. The assay is suitable for both
in-vitro
and
in-vivo
tests, and is capable of detecting 10 pg of the fungal DNA. Relatively high genetic variation was discovered between various isolates of the pathogen based on amplified fragment length polymorphism (AFLP) analyses. In addition, some isolates of
F. mangiferae
(including the accession numbers FMS-123 and NRRL 25226), were found to be quite distinct from most
F. mangiferae
accessions. Likewise, representative MMD-associated Fusaria from the Asian and American clades of the
Gibberella fujikuroi
species complex were further differentiated by AFLP analyses.</description><subject>Agriculture</subject><subject>Amplified fragment length polymorphism</subject><subject>Biomedical and Life Sciences</subject><subject>Crop diseases</subject><subject>Ecology</subject><subject>Fruits</subject><subject>Fungi</subject><subject>Fusarium</subject><subject>Genetic diversity</subject><subject>Gibberella fujikuroi</subject><subject>Life Sciences</subject><subject>Malformation disease</subject><subject>Mangifera indica</subject><subject>Pathogens</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Primers</subject><issn>0334-2123</issn><issn>1876-7184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp10L1OwzAUBWALgUQpPABbJBYWg_9ixyOqKCAVsQCr5TjXIVUSF7sZ-va4lAEhsfgO_s7V1UHokpIbSoi6TZRJQjChDBPGBJZHaEYrJbGilThGM8K5wIwyforOUloTkjUlM_T-HHpwU29j0XS2HUPqUhF8MdixDfntfYiD3XZhzP8JbILCjk2x-dj1oYVxt7fLKdnYTcN3qPMQLZyjE2_7BBc_c47elvevi0e8enl4WtytsONCb3HdKAlcKy65bupa6BKsU0qAKLn0UkNtCXGy8ZJXzqumJrQuBXUMtCs1o3yOrg97NzF8TpC2ZuiSg763I4QpGUpYRahWFcv06g9dhymO-bqsaCUFE7LKih6UiyGlCN5sYjfYuMvI7Js2h6ZNbtrsmzYyZ9ghk7IdW4i_N_8X-gK0R4DK</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>Newman, Zvi</creator><creator>Freeman, Stanley</creator><creator>Biton, Iris</creator><creator>Sa’ada, David</creator><creator>Paz, Tali</creator><creator>Maymon, Marcel</creator><creator>Lavi, Uri</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7T7</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20120701</creationdate><title>Molecular diagnosis of mango malformation disease and phylogeny of Fusarium mangiferae</title><author>Newman, Zvi ; 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Fusarium mangiferae
, is difficult to diagnose and cannot be controlled effectively. In this study a PCR diagnostic tool was developed for detection of
F. mangiferae
by generating primers which flank fungus-specific sequences. The assay is suitable for both
in-vitro
and
in-vivo
tests, and is capable of detecting 10 pg of the fungal DNA. Relatively high genetic variation was discovered between various isolates of the pathogen based on amplified fragment length polymorphism (AFLP) analyses. In addition, some isolates of
F. mangiferae
(including the accession numbers FMS-123 and NRRL 25226), were found to be quite distinct from most
F. mangiferae
accessions. Likewise, representative MMD-associated Fusaria from the Asian and American clades of the
Gibberella fujikuroi
species complex were further differentiated by AFLP analyses.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12600-012-0224-6</doi><tpages>11</tpages></addata></record> |
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| subjects | Agriculture Amplified fragment length polymorphism Biomedical and Life Sciences Crop diseases Ecology Fruits Fungi Fusarium Genetic diversity Gibberella fujikuroi Life Sciences Malformation disease Mangifera indica Pathogens Phylogenetics Phylogeny Plant Pathology Plant Sciences Polymerase chain reaction Polymorphism Primers |
| title | Molecular diagnosis of mango malformation disease and phylogeny of Fusarium mangiferae |
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