Adsorptive loss of secreted recombinant proteins in transgenic rice cell suspension cultures

Adsorptive loss of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic rice cell suspension cultures was investigated using glass flasks, plastic flasks, disposable vessels, and stainless steel vessels. When hCTLA4Ig was added to the glass flasks containing sterile AA medi...

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Veröffentlicht in:	Plant cell reports 2012-03, Vol.31 (3), p.551-560
Hauptverfasser:	Kwon, Jun-Young, Lee, Kyoung-Hoon, Cheon, Su-Hwan, Ryu, Hyun-Nam, Kim, Sun Jin, Kim, Dong-Il
Format:	Artikel
Sprache:	eng
Schlagworte:	Abatacept Adsorption Biomedical and Life Sciences Biotechnology Bovine serum albumin Cell Biology Cell culture Cell Culture Techniques Cell suspensions Cells, Cultured Cytotoxicity Glass - chemistry Humans Immunoconjugates - analysis Immunoconjugates - genetics Immunoconjugates - metabolism Immunosuppressive Agents - analysis Immunosuppressive Agents - metabolism Life Sciences Lymphocytes Lymphocytes T Original Paper Oryza - cytology Oryza - genetics Oryza - metabolism Oryza sativa pH effects Plant Biochemistry Plant Cells Plant Sciences Plants, Genetically Modified Plastics Plastics - chemistry Protein Stability Recombinant Proteins - analysis Recombinant Proteins - genetics Recombinant Proteins - metabolism Recombinant Proteins - secretion stainless steel Surface area Surface Properties Transgenic plants
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