Effects of miltefosine on the proliferation, ultrastructure, and phospholipid composition of Angomonas deanei, a trypanosomatid protozoan that harbors a symbiotic bacterium
Abstract Some trypanosomatids, such as Angomonas deanei formerly named as Crithidia deanei, present an obligatory intracellular bacterium, which maintains a mutualistic relationship with the host. Phosphatidylcholine (PC) is the major phospholipid in eukaryotes and an essential component of cell mem...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology letters 2012-08, Vol.333 (2), p.129-137 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 137 |
|---|---|
| container_issue | 2 |
| container_start_page | 129 |
| container_title | FEMS microbiology letters |
| container_volume | 333 |
| creator | de Freitas-Junior, Paulo R.G. Catta-Preta, Carolina M.C. Andrade, Iamara da Silva Cavalcanti, Danielle P. de Souza, Wanderley Einicker-Lamas, Marcelo Motta, Maria Cristina M. |
| description | Abstract
Some trypanosomatids, such as Angomonas deanei formerly named as Crithidia deanei, present an obligatory intracellular bacterium, which maintains a mutualistic relationship with the host. Phosphatidylcholine (PC) is the major phospholipid in eukaryotes and an essential component of cell membranes playing structural, biochemical, and physiological roles. However, in prokaryotes, PC is present only in those species closely associated with eukaryotes, either in symbiotic or pathogenic interactions. In trypanosomatids, the endosymbiont envelope is composed by a reduced cell wall and by two membrane units that lack sterols and present cardiolipin (CL) and PC as the major phospholipids. In this study, we tested the effects of miltefosine in A. deanei proliferation, as well as, on the ultrastrucuture and phospholipid composition considering that this drug inhibits the CTP-phosphocholine cytidyltransferase (CCT), a key enzyme in the PC biosynthesis. Besides the low effect of miltefosine in cellular proliferation, treated protozoa presented ultrastructural alterations such as plasma membrane shedding and blebbing, mitochondrial swelling, and convolutions of the endosymbiont envelope. The use of 32Pi as a tracer revealed that the production of PC, CL, and phosphatidylethanolamine decreased while phosphatidylinositol production remained stable. Mitochondrion and symbiont fractions obtained from protozoa treated with miltefosine also presented a decrease in phospholipid production, reinforcing the idea that an intensive metabolic exchange occurs between the host trypanosomatid and structures of symbiotic origin. |
| doi_str_mv | 10.1111/j.1574-6968.2012.02607.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1028018286</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6968.2012.02607.x</oup_id><sourcerecordid>2305792861</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4717-2dbef6614276631c921e340d947a886d3f78e4313216d3a540289ab1e9afce673</originalsourceid><addsrcrecordid>eNqNkd-K1DAYxYMo7rj6ChIQwYttzZ82SS-8WJZdFUa80euQpomToU1qkrI7PpMPaeqMKyiChdCE_M75TjgAQIxqXL7X-xq3vKlYx0RNECY1Igzx-u4B2NxfPAQbRLmoMOr4GXiS0h4h1BDEHoMzQliLRUs34Pu1tUbnBIOFkxuzsSE5b2DwMO8MnGMYnTVRZRf8BVzGHFXKcdF5ieYCKj_AeRdSWaOb3QB1mOZisNKr46X_EqbgVYKDUd64ooA5HmblQwpTMR3WCTl8C2qdpzLcqdiHmAqXDlPvQnYa9kpnE90yPQWPrBqTeXb6n4PPN9efrt5V249v319dbivdcMwrMvTGMoYbwhmjWHcEG9qgoWu4EoIN1HJhGoopweWg2gYR0akem05ZbRin5-DV0beE-7qYlOXkkjbjWN4QliRxESAsiGAFffEHug9L9CWdJBS1vCsQLpQ4UjqGlKKxco5uUvFQrOTaqNzLtTi5FifXRuXPRuVdkT4_DVj6yQz3wl8VFuDlCVBJq9FG5bVLvzmGWyS6tnBvjtytG83hvwPImw_bdVf09KgPy_wPdfV3_B8HMM52</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2305792861</pqid></control><display><type>article</type><title>Effects of miltefosine on the proliferation, ultrastructure, and phospholipid composition of Angomonas deanei, a trypanosomatid protozoan that harbors a symbiotic bacterium</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>de Freitas-Junior, Paulo R.G. ; Catta-Preta, Carolina M.C. ; Andrade, Iamara da Silva ; Cavalcanti, Danielle P. ; de Souza, Wanderley ; Einicker-Lamas, Marcelo ; Motta, Maria Cristina M.</creator><creatorcontrib>de Freitas-Junior, Paulo R.G. ; Catta-Preta, Carolina M.C. ; Andrade, Iamara da Silva ; Cavalcanti, Danielle P. ; de Souza, Wanderley ; Einicker-Lamas, Marcelo ; Motta, Maria Cristina M.</creatorcontrib><description>Abstract
Some trypanosomatids, such as Angomonas deanei formerly named as Crithidia deanei, present an obligatory intracellular bacterium, which maintains a mutualistic relationship with the host. Phosphatidylcholine (PC) is the major phospholipid in eukaryotes and an essential component of cell membranes playing structural, biochemical, and physiological roles. However, in prokaryotes, PC is present only in those species closely associated with eukaryotes, either in symbiotic or pathogenic interactions. In trypanosomatids, the endosymbiont envelope is composed by a reduced cell wall and by two membrane units that lack sterols and present cardiolipin (CL) and PC as the major phospholipids. In this study, we tested the effects of miltefosine in A. deanei proliferation, as well as, on the ultrastrucuture and phospholipid composition considering that this drug inhibits the CTP-phosphocholine cytidyltransferase (CCT), a key enzyme in the PC biosynthesis. Besides the low effect of miltefosine in cellular proliferation, treated protozoa presented ultrastructural alterations such as plasma membrane shedding and blebbing, mitochondrial swelling, and convolutions of the endosymbiont envelope. The use of 32Pi as a tracer revealed that the production of PC, CL, and phosphatidylethanolamine decreased while phosphatidylinositol production remained stable. Mitochondrion and symbiont fractions obtained from protozoa treated with miltefosine also presented a decrease in phospholipid production, reinforcing the idea that an intensive metabolic exchange occurs between the host trypanosomatid and structures of symbiotic origin.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2012.02607.x</identifier><identifier>PMID: 22651853</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Angomonas deanei ; Bacteria ; Bacteria - drug effects ; Bacteria - growth & development ; Biological and medical sciences ; Biosynthesis ; Cardiolipin ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Cell Membrane - ultrastructure ; Cell membranes ; Cell Wall - drug effects ; Cell Wall - metabolism ; Cell walls ; Choline-Phosphate Cytidylyltransferase - metabolism ; Composition ; Crithidia - drug effects ; Crithidia - metabolism ; Crithidia - microbiology ; Crithidia - ultrastructure ; endosymbiosis ; Eukaryotes ; Fundamental and applied biological sciences. Psychology ; Lecithin ; Microbiology ; Microscopy, Electron, Transmission ; Miltefosine ; Mitochondria ; Mitochondria - drug effects ; Mitochondria - ultrastructure ; Phosphatidylcholine ; Phosphatidylcholines - biosynthesis ; Phosphatidylethanolamine ; Phosphatidylinositol ; Phosphocholine ; phospholipid biosynthesis ; Phospholipid composition ; Phospholipids ; Phosphorus Isotopes - metabolism ; Phosphorylcholine - analogs & derivatives ; Phosphorylcholine - pharmacology ; Prokaryotes ; Protozoa ; Sterols ; Symbiosis ; trypanosomatid protozoa ; Ultrastructure</subject><ispartof>FEMS microbiology letters, 2012-08, Vol.333 (2), p.129-137</ispartof><rights>Copyright © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved 2012</rights><rights>2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2015 INIST-CNRS</rights><rights>2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</rights><rights>Copyright © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4717-2dbef6614276631c921e340d947a886d3f78e4313216d3a540289ab1e9afce673</citedby><cites>FETCH-LOGICAL-c4717-2dbef6614276631c921e340d947a886d3f78e4313216d3a540289ab1e9afce673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.2012.02607.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.2012.02607.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26150895$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22651853$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Freitas-Junior, Paulo R.G.</creatorcontrib><creatorcontrib>Catta-Preta, Carolina M.C.</creatorcontrib><creatorcontrib>Andrade, Iamara da Silva</creatorcontrib><creatorcontrib>Cavalcanti, Danielle P.</creatorcontrib><creatorcontrib>de Souza, Wanderley</creatorcontrib><creatorcontrib>Einicker-Lamas, Marcelo</creatorcontrib><creatorcontrib>Motta, Maria Cristina M.</creatorcontrib><title>Effects of miltefosine on the proliferation, ultrastructure, and phospholipid composition of Angomonas deanei, a trypanosomatid protozoan that harbors a symbiotic bacterium</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Some trypanosomatids, such as Angomonas deanei formerly named as Crithidia deanei, present an obligatory intracellular bacterium, which maintains a mutualistic relationship with the host. Phosphatidylcholine (PC) is the major phospholipid in eukaryotes and an essential component of cell membranes playing structural, biochemical, and physiological roles. However, in prokaryotes, PC is present only in those species closely associated with eukaryotes, either in symbiotic or pathogenic interactions. In trypanosomatids, the endosymbiont envelope is composed by a reduced cell wall and by two membrane units that lack sterols and present cardiolipin (CL) and PC as the major phospholipids. In this study, we tested the effects of miltefosine in A. deanei proliferation, as well as, on the ultrastrucuture and phospholipid composition considering that this drug inhibits the CTP-phosphocholine cytidyltransferase (CCT), a key enzyme in the PC biosynthesis. Besides the low effect of miltefosine in cellular proliferation, treated protozoa presented ultrastructural alterations such as plasma membrane shedding and blebbing, mitochondrial swelling, and convolutions of the endosymbiont envelope. The use of 32Pi as a tracer revealed that the production of PC, CL, and phosphatidylethanolamine decreased while phosphatidylinositol production remained stable. Mitochondrion and symbiont fractions obtained from protozoa treated with miltefosine also presented a decrease in phospholipid production, reinforcing the idea that an intensive metabolic exchange occurs between the host trypanosomatid and structures of symbiotic origin.</description><subject>Angomonas deanei</subject><subject>Bacteria</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - growth & development</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Cardiolipin</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane - ultrastructure</subject><subject>Cell membranes</subject><subject>Cell Wall - drug effects</subject><subject>Cell Wall - metabolism</subject><subject>Cell walls</subject><subject>Choline-Phosphate Cytidylyltransferase - metabolism</subject><subject>Composition</subject><subject>Crithidia - drug effects</subject><subject>Crithidia - metabolism</subject><subject>Crithidia - microbiology</subject><subject>Crithidia - ultrastructure</subject><subject>endosymbiosis</subject><subject>Eukaryotes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lecithin</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Transmission</subject><subject>Miltefosine</subject><subject>Mitochondria</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - ultrastructure</subject><subject>Phosphatidylcholine</subject><subject>Phosphatidylcholines - biosynthesis</subject><subject>Phosphatidylethanolamine</subject><subject>Phosphatidylinositol</subject><subject>Phosphocholine</subject><subject>phospholipid biosynthesis</subject><subject>Phospholipid composition</subject><subject>Phospholipids</subject><subject>Phosphorus Isotopes - metabolism</subject><subject>Phosphorylcholine - analogs & derivatives</subject><subject>Phosphorylcholine - pharmacology</subject><subject>Prokaryotes</subject><subject>Protozoa</subject><subject>Sterols</subject><subject>Symbiosis</subject><subject>trypanosomatid protozoa</subject><subject>Ultrastructure</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkd-K1DAYxYMo7rj6ChIQwYttzZ82SS-8WJZdFUa80euQpomToU1qkrI7PpMPaeqMKyiChdCE_M75TjgAQIxqXL7X-xq3vKlYx0RNECY1Igzx-u4B2NxfPAQbRLmoMOr4GXiS0h4h1BDEHoMzQliLRUs34Pu1tUbnBIOFkxuzsSE5b2DwMO8MnGMYnTVRZRf8BVzGHFXKcdF5ieYCKj_AeRdSWaOb3QB1mOZisNKr46X_EqbgVYKDUd64ooA5HmblQwpTMR3WCTl8C2qdpzLcqdiHmAqXDlPvQnYa9kpnE90yPQWPrBqTeXb6n4PPN9efrt5V249v319dbivdcMwrMvTGMoYbwhmjWHcEG9qgoWu4EoIN1HJhGoopweWg2gYR0akem05ZbRin5-DV0beE-7qYlOXkkjbjWN4QliRxESAsiGAFffEHug9L9CWdJBS1vCsQLpQ4UjqGlKKxco5uUvFQrOTaqNzLtTi5FifXRuXPRuVdkT4_DVj6yQz3wl8VFuDlCVBJq9FG5bVLvzmGWyS6tnBvjtytG83hvwPImw_bdVf09KgPy_wPdfV3_B8HMM52</recordid><startdate>201208</startdate><enddate>201208</enddate><creator>de Freitas-Junior, Paulo R.G.</creator><creator>Catta-Preta, Carolina M.C.</creator><creator>Andrade, Iamara da Silva</creator><creator>Cavalcanti, Danielle P.</creator><creator>de Souza, Wanderley</creator><creator>Einicker-Lamas, Marcelo</creator><creator>Motta, Maria Cristina M.</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201208</creationdate><title>Effects of miltefosine on the proliferation, ultrastructure, and phospholipid composition of Angomonas deanei, a trypanosomatid protozoan that harbors a symbiotic bacterium</title><author>de Freitas-Junior, Paulo R.G. ; Catta-Preta, Carolina M.C. ; Andrade, Iamara da Silva ; Cavalcanti, Danielle P. ; de Souza, Wanderley ; Einicker-Lamas, Marcelo ; Motta, Maria Cristina M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4717-2dbef6614276631c921e340d947a886d3f78e4313216d3a540289ab1e9afce673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Angomonas deanei</topic><topic>Bacteria</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - growth & development</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Cardiolipin</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane - ultrastructure</topic><topic>Cell membranes</topic><topic>Cell Wall - drug effects</topic><topic>Cell Wall - metabolism</topic><topic>Cell walls</topic><topic>Choline-Phosphate Cytidylyltransferase - metabolism</topic><topic>Composition</topic><topic>Crithidia - drug effects</topic><topic>Crithidia - metabolism</topic><topic>Crithidia - microbiology</topic><topic>Crithidia - ultrastructure</topic><topic>endosymbiosis</topic><topic>Eukaryotes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lecithin</topic><topic>Microbiology</topic><topic>Microscopy, Electron, Transmission</topic><topic>Miltefosine</topic><topic>Mitochondria</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - ultrastructure</topic><topic>Phosphatidylcholine</topic><topic>Phosphatidylcholines - biosynthesis</topic><topic>Phosphatidylethanolamine</topic><topic>Phosphatidylinositol</topic><topic>Phosphocholine</topic><topic>phospholipid biosynthesis</topic><topic>Phospholipid composition</topic><topic>Phospholipids</topic><topic>Phosphorus Isotopes - metabolism</topic><topic>Phosphorylcholine - analogs & derivatives</topic><topic>Phosphorylcholine - pharmacology</topic><topic>Prokaryotes</topic><topic>Protozoa</topic><topic>Sterols</topic><topic>Symbiosis</topic><topic>trypanosomatid protozoa</topic><topic>Ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Freitas-Junior, Paulo R.G.</creatorcontrib><creatorcontrib>Catta-Preta, Carolina M.C.</creatorcontrib><creatorcontrib>Andrade, Iamara da Silva</creatorcontrib><creatorcontrib>Cavalcanti, Danielle P.</creatorcontrib><creatorcontrib>de Souza, Wanderley</creatorcontrib><creatorcontrib>Einicker-Lamas, Marcelo</creatorcontrib><creatorcontrib>Motta, Maria Cristina M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Freitas-Junior, Paulo R.G.</au><au>Catta-Preta, Carolina M.C.</au><au>Andrade, Iamara da Silva</au><au>Cavalcanti, Danielle P.</au><au>de Souza, Wanderley</au><au>Einicker-Lamas, Marcelo</au><au>Motta, Maria Cristina M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of miltefosine on the proliferation, ultrastructure, and phospholipid composition of Angomonas deanei, a trypanosomatid protozoan that harbors a symbiotic bacterium</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2012-08</date><risdate>2012</risdate><volume>333</volume><issue>2</issue><spage>129</spage><epage>137</epage><pages>129-137</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Some trypanosomatids, such as Angomonas deanei formerly named as Crithidia deanei, present an obligatory intracellular bacterium, which maintains a mutualistic relationship with the host. Phosphatidylcholine (PC) is the major phospholipid in eukaryotes and an essential component of cell membranes playing structural, biochemical, and physiological roles. However, in prokaryotes, PC is present only in those species closely associated with eukaryotes, either in symbiotic or pathogenic interactions. In trypanosomatids, the endosymbiont envelope is composed by a reduced cell wall and by two membrane units that lack sterols and present cardiolipin (CL) and PC as the major phospholipids. In this study, we tested the effects of miltefosine in A. deanei proliferation, as well as, on the ultrastrucuture and phospholipid composition considering that this drug inhibits the CTP-phosphocholine cytidyltransferase (CCT), a key enzyme in the PC biosynthesis. Besides the low effect of miltefosine in cellular proliferation, treated protozoa presented ultrastructural alterations such as plasma membrane shedding and blebbing, mitochondrial swelling, and convolutions of the endosymbiont envelope. The use of 32Pi as a tracer revealed that the production of PC, CL, and phosphatidylethanolamine decreased while phosphatidylinositol production remained stable. Mitochondrion and symbiont fractions obtained from protozoa treated with miltefosine also presented a decrease in phospholipid production, reinforcing the idea that an intensive metabolic exchange occurs between the host trypanosomatid and structures of symbiotic origin.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22651853</pmid><doi>10.1111/j.1574-6968.2012.02607.x</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2012-08, Vol.333 (2), p.129-137 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1028018286 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Angomonas deanei Bacteria Bacteria - drug effects Bacteria - growth & development Biological and medical sciences Biosynthesis Cardiolipin Cell Membrane - drug effects Cell Membrane - metabolism Cell Membrane - ultrastructure Cell membranes Cell Wall - drug effects Cell Wall - metabolism Cell walls Choline-Phosphate Cytidylyltransferase - metabolism Composition Crithidia - drug effects Crithidia - metabolism Crithidia - microbiology Crithidia - ultrastructure endosymbiosis Eukaryotes Fundamental and applied biological sciences. Psychology Lecithin Microbiology Microscopy, Electron, Transmission Miltefosine Mitochondria Mitochondria - drug effects Mitochondria - ultrastructure Phosphatidylcholine Phosphatidylcholines - biosynthesis Phosphatidylethanolamine Phosphatidylinositol Phosphocholine phospholipid biosynthesis Phospholipid composition Phospholipids Phosphorus Isotopes - metabolism Phosphorylcholine - analogs & derivatives Phosphorylcholine - pharmacology Prokaryotes Protozoa Sterols Symbiosis trypanosomatid protozoa Ultrastructure |
| title | Effects of miltefosine on the proliferation, ultrastructure, and phospholipid composition of Angomonas deanei, a trypanosomatid protozoan that harbors a symbiotic bacterium |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T13%3A48%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20miltefosine%20on%20the%20proliferation,%20ultrastructure,%20and%20phospholipid%20composition%20of%20Angomonas%20deanei,%20a%20trypanosomatid%20protozoan%20that%20harbors%20a%20symbiotic%20bacterium&rft.jtitle=FEMS%20microbiology%20letters&rft.au=de%20Freitas-Junior,%20Paulo%20R.G.&rft.date=2012-08&rft.volume=333&rft.issue=2&rft.spage=129&rft.epage=137&rft.pages=129-137&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1111/j.1574-6968.2012.02607.x&rft_dat=%3Cproquest_cross%3E2305792861%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2305792861&rft_id=info:pmid/22651853&rft_oup_id=10.1111/j.1574-6968.2012.02607.x&rfr_iscdi=true |