A Simple Method for the Determination of Malachite Green and Leucomalachite Green Residues in Fish by a Modified QuEChERS Extraction and LC/MS/MS
A simple method using LC/MS/MS was developed and validated to determine residues of malachite green (MG) and leucomalachite green (LMG) in fish fillet. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) technique was used to perform the sample preparation. The optimal extraction a...
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| Veröffentlicht in: | Journal of AOAC International 2012-05, Vol.95 (3), p.913-922 |
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| container_title | Journal of AOAC International |
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| creator | HASHIMOTO, Juliana C PASCHOAL, Jonas A. R QUEIROZ, Sonia C. N FERRACINI, Vera L ASSALIN, Márcia R RYES, Felix G. R |
| description | A simple method using LC/MS/MS was developed and validated to determine residues of malachite green (MG) and leucomalachite green (LMG) in fish fillet. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) technique was used to perform the sample preparation. The optimal extraction and cleanup conditions were established using an experimental design. The validation parameters obtained to determine both MG and LMG complied with the requirements established by regulatory agencies for the presence of such substances in fish, which establish that the method must attain, at least, a minimum required performance limit of 2 ng/g. The accuracy values ranged between 95 and 107%, and the precision values were lower than 11.2%. The method was used in the analysis of tilapia samples (n = 20) commercialized in Campinas, SP, Brazil. None of the samples presented detectable levels of MG or LMG residues. |
| doi_str_mv | 10.5740/jaoacint.11-140 |
| format | Article |
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R ; QUEIROZ, Sonia C. N ; FERRACINI, Vera L ; ASSALIN, Márcia R ; RYES, Felix G. R</creator><creatorcontrib>HASHIMOTO, Juliana C ; PASCHOAL, Jonas A. R ; QUEIROZ, Sonia C. N ; FERRACINI, Vera L ; ASSALIN, Márcia R ; RYES, Felix G. R</creatorcontrib><description>A simple method using LC/MS/MS was developed and validated to determine residues of malachite green (MG) and leucomalachite green (LMG) in fish fillet. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) technique was used to perform the sample preparation. The optimal extraction and cleanup conditions were established using an experimental design. The validation parameters obtained to determine both MG and LMG complied with the requirements established by regulatory agencies for the presence of such substances in fish, which establish that the method must attain, at least, a minimum required performance limit of 2 ng/g. The accuracy values ranged between 95 and 107%, and the precision values were lower than 11.2%. The method was used in the analysis of tilapia samples (n = 20) commercialized in Campinas, SP, Brazil. None of the samples presented detectable levels of MG or LMG residues.</description><identifier>ISSN: 1060-3271</identifier><identifier>EISSN: 1944-7922</identifier><identifier>DOI: 10.5740/jaoacint.11-140</identifier><identifier>PMID: 22816282</identifier><language>eng</language><publisher>Gaithersburg, MD: AOAC International</publisher><subject>Animals ; Biological and medical sciences ; Chemical properties ; Chromatography, Liquid - methods ; Fishes ; Food Contamination - analysis ; Food engineering ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; Identification and classification ; Liquid chromatography ; Malachite ; Mass spectrometry ; Methods ; Rosaniline Dyes - analysis ; Rosaniline Dyes - isolation & purification ; Seafood - analysis ; Tandem Mass Spectrometry - methods</subject><ispartof>Journal of AOAC International, 2012-05, Vol.95 (3), p.913-922</ispartof><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2012 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-81e9a61b6c84f9c027cceaf8e620c477ae3d3c9333fb2a9c77258a747cba6f7d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25991119$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22816282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HASHIMOTO, Juliana C</creatorcontrib><creatorcontrib>PASCHOAL, Jonas A. R</creatorcontrib><creatorcontrib>QUEIROZ, Sonia C. N</creatorcontrib><creatorcontrib>FERRACINI, Vera L</creatorcontrib><creatorcontrib>ASSALIN, Márcia R</creatorcontrib><creatorcontrib>RYES, Felix G. R</creatorcontrib><title>A Simple Method for the Determination of Malachite Green and Leucomalachite Green Residues in Fish by a Modified QuEChERS Extraction and LC/MS/MS</title><title>Journal of AOAC International</title><addtitle>J AOAC Int</addtitle><description>A simple method using LC/MS/MS was developed and validated to determine residues of malachite green (MG) and leucomalachite green (LMG) in fish fillet. A modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) technique was used to perform the sample preparation. The optimal extraction and cleanup conditions were established using an experimental design. The validation parameters obtained to determine both MG and LMG complied with the requirements established by regulatory agencies for the presence of such substances in fish, which establish that the method must attain, at least, a minimum required performance limit of 2 ng/g. The accuracy values ranged between 95 and 107%, and the precision values were lower than 11.2%. The method was used in the analysis of tilapia samples (n = 20) commercialized in Campinas, SP, Brazil. None of the samples presented detectable levels of MG or LMG residues.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chemical properties</subject><subject>Chromatography, Liquid - methods</subject><subject>Fishes</subject><subject>Food Contamination - analysis</subject><subject>Food engineering</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Identification and classification</subject><subject>Liquid chromatography</subject><subject>Malachite</subject><subject>Mass spectrometry</subject><subject>Methods</subject><subject>Rosaniline Dyes - analysis</subject><subject>Rosaniline Dyes - isolation & purification</subject><subject>Seafood - analysis</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>1060-3271</issn><issn>1944-7922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkVFv0zAUhSMEYmPwzBvyCxIvaW3HtePHqnQDqRVihefoxrkmnhK72InEfgb_GG_tQEK25Cv7O0dHPkXxltHFSgm6vIMAxvlpwVjJBH1WXDItRKk058_zTCUtK67YRfEqpTtKBZOUvywuOK-Z5DW_LH6vycGNxwHJHqc-dMSGSKYeyUecMI7Ow-SCJ8GSPQxgejchuYmInoDvyA5nE8b_Hm4xuW7GRJwn1y71pL0nQPahc9ZhR77O202_vT2Q7a8pgnm0f_TaLPeHvF8XLywMCd-cz6vi-_X22-ZTufty83mz3pVGUDWVNUMNkrXS1MJqQ7kyBsHWKDk1QinAqquMrqrKthy0UYqvalBCmRakVV11VXw4-R5j-JnjTs3oksFhAI9hTg3LljJ_k6gzujihP2DAxnkbHpLn1eHoTPBoXb5fV1RTWfOVzILlSWBiSCmibY7RjRDvs2vzUFzzVFzDWJOLy4p35zRzO2L3l39qKgPvzwAkA4ON4I1L_7iV1owxXf0B1GuiNA</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>HASHIMOTO, Juliana C</creator><creator>PASCHOAL, Jonas A. 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Psychology</topic><topic>General aspects</topic><topic>Identification and classification</topic><topic>Liquid chromatography</topic><topic>Malachite</topic><topic>Mass spectrometry</topic><topic>Methods</topic><topic>Rosaniline Dyes - analysis</topic><topic>Rosaniline Dyes - isolation & purification</topic><topic>Seafood - analysis</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HASHIMOTO, Juliana C</creatorcontrib><creatorcontrib>PASCHOAL, Jonas A. R</creatorcontrib><creatorcontrib>QUEIROZ, Sonia C. N</creatorcontrib><creatorcontrib>FERRACINI, Vera L</creatorcontrib><creatorcontrib>ASSALIN, Márcia R</creatorcontrib><creatorcontrib>RYES, Felix G. 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The validation parameters obtained to determine both MG and LMG complied with the requirements established by regulatory agencies for the presence of such substances in fish, which establish that the method must attain, at least, a minimum required performance limit of 2 ng/g. The accuracy values ranged between 95 and 107%, and the precision values were lower than 11.2%. The method was used in the analysis of tilapia samples (n = 20) commercialized in Campinas, SP, Brazil. None of the samples presented detectable levels of MG or LMG residues.</abstract><cop>Gaithersburg, MD</cop><pub>AOAC International</pub><pmid>22816282</pmid><doi>10.5740/jaoacint.11-140</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Biological and medical sciences Chemical properties Chromatography, Liquid - methods Fishes Food Contamination - analysis Food engineering Food industries Fundamental and applied biological sciences. Psychology General aspects Identification and classification Liquid chromatography Malachite Mass spectrometry Methods Rosaniline Dyes - analysis Rosaniline Dyes - isolation & purification Seafood - analysis Tandem Mass Spectrometry - methods |
| title | A Simple Method for the Determination of Malachite Green and Leucomalachite Green Residues in Fish by a Modified QuEChERS Extraction and LC/MS/MS |
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