Inducible Prrxl1-CreER(T2) recombination activity in the somatosensory afferent pathway
Prrxl1-CreER(T2) transgenic mice expressing tamoxifen-inducible Cre recombinase were generated by modifying a Prrxl1-containing BAC clone. Cre recombination activity was examined in Prrxl1-CreER(T2); Rosa26 reporter mice at various embryonic and postnatal stages. Pregnant mice were treated with a si...
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| Veröffentlicht in: | Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2012-07, Vol.50 (7), p.552-560 |
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| container_title | Genesis (New York, N.Y. : 2000) |
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| creator | Hu, Ze-Lan Huang, Ying Tao, Xiao-Rong Qi, Zheng-Han Chen, Jia-Yin Ding, Yu-Qiang |
| description | Prrxl1-CreER(T2) transgenic mice expressing tamoxifen-inducible Cre recombinase were generated by modifying a Prrxl1-containing BAC clone. Cre recombination activity was examined in Prrxl1-CreER(T2); Rosa26 reporter mice at various embryonic and postnatal stages. Pregnant mice were treated with a single dose of tamoxifen at embryonic day (E) 9.5 or E12.5, and X-gal staining was performed 2 days later. Strong X-gal staining was observed in the somatosensory ganglia (e.g., dorsal root and trigeminal ganglia) and the first central sites for processing somatosensory information (e.g., spinal dorsal horn and trigeminal nerve-associated nuclei). When tamoxifen was administered at postnatal day (P) 20 or in adulthood (P120), strong Cre recombination activity was present in the primary somatosensory ganglia, while weak Cre recombination activity was found in the spinal dorsal horn, mesencephalic trigeminal nucleus, principal sensory trigeminal nucleus, and spinal trigeminal nucleus. This mouse line provides a useful tool for exploring genes' functions in the somatosensory system in a time-controlled way. |
| doi_str_mv | 10.1002/dvg.22020 |
| format | Article |
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Cre recombination activity was examined in Prrxl1-CreER(T2); Rosa26 reporter mice at various embryonic and postnatal stages. Pregnant mice were treated with a single dose of tamoxifen at embryonic day (E) 9.5 or E12.5, and X-gal staining was performed 2 days later. Strong X-gal staining was observed in the somatosensory ganglia (e.g., dorsal root and trigeminal ganglia) and the first central sites for processing somatosensory information (e.g., spinal dorsal horn and trigeminal nerve-associated nuclei). When tamoxifen was administered at postnatal day (P) 20 or in adulthood (P120), strong Cre recombination activity was present in the primary somatosensory ganglia, while weak Cre recombination activity was found in the spinal dorsal horn, mesencephalic trigeminal nucleus, principal sensory trigeminal nucleus, and spinal trigeminal nucleus. This mouse line provides a useful tool for exploring genes' functions in the somatosensory system in a time-controlled way.</description><identifier>EISSN: 1526-968X</identifier><identifier>DOI: 10.1002/dvg.22020</identifier><identifier>PMID: 22368151</identifier><language>eng</language><publisher>United States</publisher><subject>Afferent Pathways - embryology ; Afferent Pathways - physiology ; Animals ; Chromosomes, Artificial, Bacterial ; Embryo, Mammalian ; Female ; Founder Effect ; Gene Expression Regulation, Developmental - drug effects ; Genes, Reporter ; Homeodomain Proteins - genetics ; Integrases - genetics ; Mice ; Mice, Transgenic ; Nerve Tissue Proteins - genetics ; Pregnancy ; Promoter Regions, Genetic ; Proteins - genetics ; Recombination, Genetic - drug effects ; RNA, Untranslated ; Somatosensory Cortex - embryology ; Somatosensory Cortex - physiology ; Spinal Nerve Roots - embryology ; Spinal Nerve Roots - physiology ; Tamoxifen - administration & dosage ; Time Factors ; Transcription Factors - genetics ; Trigeminal Ganglion - embryology ; Trigeminal Ganglion - physiology</subject><ispartof>Genesis (New York, N.Y. : 2000), 2012-07, Vol.50 (7), p.552-560</ispartof><rights>Copyright © 2012 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22368151$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Ze-Lan</creatorcontrib><creatorcontrib>Huang, Ying</creatorcontrib><creatorcontrib>Tao, Xiao-Rong</creatorcontrib><creatorcontrib>Qi, Zheng-Han</creatorcontrib><creatorcontrib>Chen, Jia-Yin</creatorcontrib><creatorcontrib>Ding, Yu-Qiang</creatorcontrib><title>Inducible Prrxl1-CreER(T2) recombination activity in the somatosensory afferent pathway</title><title>Genesis (New York, N.Y. : 2000)</title><addtitle>Genesis</addtitle><description>Prrxl1-CreER(T2) transgenic mice expressing tamoxifen-inducible Cre recombinase were generated by modifying a Prrxl1-containing BAC clone. Cre recombination activity was examined in Prrxl1-CreER(T2); Rosa26 reporter mice at various embryonic and postnatal stages. Pregnant mice were treated with a single dose of tamoxifen at embryonic day (E) 9.5 or E12.5, and X-gal staining was performed 2 days later. Strong X-gal staining was observed in the somatosensory ganglia (e.g., dorsal root and trigeminal ganglia) and the first central sites for processing somatosensory information (e.g., spinal dorsal horn and trigeminal nerve-associated nuclei). When tamoxifen was administered at postnatal day (P) 20 or in adulthood (P120), strong Cre recombination activity was present in the primary somatosensory ganglia, while weak Cre recombination activity was found in the spinal dorsal horn, mesencephalic trigeminal nucleus, principal sensory trigeminal nucleus, and spinal trigeminal nucleus. This mouse line provides a useful tool for exploring genes' functions in the somatosensory system in a time-controlled way.</description><subject>Afferent Pathways - embryology</subject><subject>Afferent Pathways - physiology</subject><subject>Animals</subject><subject>Chromosomes, Artificial, Bacterial</subject><subject>Embryo, Mammalian</subject><subject>Female</subject><subject>Founder Effect</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Genes, Reporter</subject><subject>Homeodomain Proteins - genetics</subject><subject>Integrases - genetics</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Pregnancy</subject><subject>Promoter Regions, Genetic</subject><subject>Proteins - genetics</subject><subject>Recombination, Genetic - drug effects</subject><subject>RNA, Untranslated</subject><subject>Somatosensory Cortex - embryology</subject><subject>Somatosensory Cortex - physiology</subject><subject>Spinal Nerve Roots - embryology</subject><subject>Spinal Nerve Roots - physiology</subject><subject>Tamoxifen - administration & dosage</subject><subject>Time Factors</subject><subject>Transcription Factors - genetics</subject><subject>Trigeminal Ganglion - embryology</subject><subject>Trigeminal Ganglion - physiology</subject><issn>1526-968X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEtLAzEYAIMgtlYP_gHJsR5Wky9NNnuUUrVQUKSityWvtZF9mWSr--8VrKe5DHMYhC4ouaaEwI3dv18DECBHaEo5iKwQ8m2CTmP8IIRwCXCCJgBMSMrpFL2uWzsYr2uHn0L4rmm2DG71PN_CFQ7OdI32rUq-a7Eyye99GrFvcdo5HLtGpS66NnZhxKqqXHBtwr1Kuy81nqHjStXRnR84Qy93q-3yIds83q-Xt5uspyBSZqWB3PGcG8O5rEBqsGBYrpQwkheWGQcFk7rKqS5yEAurQBeSSE2ctSDYDM3_un3oPgcXU9n4aFxdq9Z1QywpgZwsgFH2q14e1EE3zpZ98I0KY_k_g_0ARaZerA</recordid><startdate>201207</startdate><enddate>201207</enddate><creator>Hu, Ze-Lan</creator><creator>Huang, Ying</creator><creator>Tao, Xiao-Rong</creator><creator>Qi, Zheng-Han</creator><creator>Chen, Jia-Yin</creator><creator>Ding, Yu-Qiang</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201207</creationdate><title>Inducible Prrxl1-CreER(T2) recombination activity in the somatosensory afferent pathway</title><author>Hu, Ze-Lan ; Huang, Ying ; Tao, Xiao-Rong ; Qi, Zheng-Han ; Chen, Jia-Yin ; Ding, Yu-Qiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-d8c27e575cc558f28b2d2c37aa6c859d3ce2938bf71b97264da2b9808b0edd263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Afferent Pathways - embryology</topic><topic>Afferent Pathways - physiology</topic><topic>Animals</topic><topic>Chromosomes, Artificial, Bacterial</topic><topic>Embryo, Mammalian</topic><topic>Female</topic><topic>Founder Effect</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Genes, Reporter</topic><topic>Homeodomain Proteins - genetics</topic><topic>Integrases - genetics</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Pregnancy</topic><topic>Promoter Regions, Genetic</topic><topic>Proteins - genetics</topic><topic>Recombination, Genetic - drug effects</topic><topic>RNA, Untranslated</topic><topic>Somatosensory Cortex - embryology</topic><topic>Somatosensory Cortex - physiology</topic><topic>Spinal Nerve Roots - embryology</topic><topic>Spinal Nerve Roots - physiology</topic><topic>Tamoxifen - administration & dosage</topic><topic>Time Factors</topic><topic>Transcription Factors - genetics</topic><topic>Trigeminal Ganglion - embryology</topic><topic>Trigeminal Ganglion - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Ze-Lan</creatorcontrib><creatorcontrib>Huang, Ying</creatorcontrib><creatorcontrib>Tao, Xiao-Rong</creatorcontrib><creatorcontrib>Qi, Zheng-Han</creatorcontrib><creatorcontrib>Chen, Jia-Yin</creatorcontrib><creatorcontrib>Ding, Yu-Qiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Genesis (New York, N.Y. : 2000)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Ze-Lan</au><au>Huang, Ying</au><au>Tao, Xiao-Rong</au><au>Qi, Zheng-Han</au><au>Chen, Jia-Yin</au><au>Ding, Yu-Qiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inducible Prrxl1-CreER(T2) recombination activity in the somatosensory afferent pathway</atitle><jtitle>Genesis (New York, N.Y. : 2000)</jtitle><addtitle>Genesis</addtitle><date>2012-07</date><risdate>2012</risdate><volume>50</volume><issue>7</issue><spage>552</spage><epage>560</epage><pages>552-560</pages><eissn>1526-968X</eissn><abstract>Prrxl1-CreER(T2) transgenic mice expressing tamoxifen-inducible Cre recombinase were generated by modifying a Prrxl1-containing BAC clone. Cre recombination activity was examined in Prrxl1-CreER(T2); Rosa26 reporter mice at various embryonic and postnatal stages. Pregnant mice were treated with a single dose of tamoxifen at embryonic day (E) 9.5 or E12.5, and X-gal staining was performed 2 days later. Strong X-gal staining was observed in the somatosensory ganglia (e.g., dorsal root and trigeminal ganglia) and the first central sites for processing somatosensory information (e.g., spinal dorsal horn and trigeminal nerve-associated nuclei). When tamoxifen was administered at postnatal day (P) 20 or in adulthood (P120), strong Cre recombination activity was present in the primary somatosensory ganglia, while weak Cre recombination activity was found in the spinal dorsal horn, mesencephalic trigeminal nucleus, principal sensory trigeminal nucleus, and spinal trigeminal nucleus. This mouse line provides a useful tool for exploring genes' functions in the somatosensory system in a time-controlled way.</abstract><cop>United States</cop><pmid>22368151</pmid><doi>10.1002/dvg.22020</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Afferent Pathways - embryology Afferent Pathways - physiology Animals Chromosomes, Artificial, Bacterial Embryo, Mammalian Female Founder Effect Gene Expression Regulation, Developmental - drug effects Genes, Reporter Homeodomain Proteins - genetics Integrases - genetics Mice Mice, Transgenic Nerve Tissue Proteins - genetics Pregnancy Promoter Regions, Genetic Proteins - genetics Recombination, Genetic - drug effects RNA, Untranslated Somatosensory Cortex - embryology Somatosensory Cortex - physiology Spinal Nerve Roots - embryology Spinal Nerve Roots - physiology Tamoxifen - administration & dosage Time Factors Transcription Factors - genetics Trigeminal Ganglion - embryology Trigeminal Ganglion - physiology |
| title | Inducible Prrxl1-CreER(T2) recombination activity in the somatosensory afferent pathway |
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