Non-culture detection of Streptococcus agalactiae (Lancefield group B Streptococcus) in clinical samples by real-time PCR

A real-time PCR assay targeting the cylB gene was developed to detect Streptococcus agalactiae [Lancefield group B Streptococcus (GBS)] from clinical samples. A total of 110 blood culture-negative samples [75 cerebrospinal fluid (CSF) and 35 EDTA blood samples] from neonates with probable GBS sepsis...

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Veröffentlicht in:	Journal of medical microbiology 2012-08, Vol.61 (8), p.1086-1090
Hauptverfasser:	DE ZOYSA, Aruni, EDWARDS, Kirstin, GHARBIA, Saheer, UNDERWOOD, Anthony, CHARLETT, Andre, EFSTRATIOU, Androulla
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological Techniques - methods Bacteriology Biological and medical sciences Blood - microbiology Cerebrospinal Fluid - microbiology Fundamental and applied biological sciences. Psychology Humans Infant, Newborn Infectious diseases Medical sciences Meningitis - diagnosis Meningitis - microbiology Microbiology Miscellaneous Molecular Diagnostic Techniques - methods Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity Sepsis - diagnosis Sepsis - microbiology Streptococcal Infections - diagnosis Streptococcal Infections - microbiology Streptococcus agalactiae - genetics Streptococcus agalactiae - isolation & purification
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