Expression of HSP27 in CA1 area of hippocampus in temporal lobe epilepsy model
To explore the expression of heat shock protein 27 (HSP27) in the CA1 area of hippocampus in temporal lobe epilepsy (TLE) so as to elucidate the relationship between HSP27 and epileptogenesis of TLE. The model of TLE was induced by lithium-pilocarpine in the experiment group. And the rats were furth...
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| Veröffentlicht in: | Zhong hua yi xue za zhi 2012-03, Vol.92 (9), p.642-644 |
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| container_title | Zhong hua yi xue za zhi |
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| creator | Yang, Pu Wu, Liu-yi-wen Xiao, Bo Chen, Ying-zhu Wu, Xiao-mei Huang, Zhi-ling |
| description | To explore the expression of heat shock protein 27 (HSP27) in the CA1 area of hippocampus in temporal lobe epilepsy (TLE) so as to elucidate the relationship between HSP27 and epileptogenesis of TLE.
The model of TLE was induced by lithium-pilocarpine in the experiment group. And the rats were further divided into the STLE and non-STLE groups based upon the absence or presence of recurrent spontaneous seizure in the next 30 days. Total protein fractions from CA1 area of hippocampus were successively obtained through tissue homogenates abstraction. The HSP27 expression in the CA1 area of hippocamp from three groups was semi-quantitatively analyzed by Western blot. And the expression of HSP27 in CA1 area was detected by pre-embedding immunogold electron microscopy.
Expression of HSP27 in the hippocampus CA1 area as detected by Western blot was in accord with that by immunogold electron microscopy. Relative optical density values were 0.912 ± 0.011, 0.431 ± 0.011 and 0.428 ± 0.010 respectively. And gold particle |
| doi_str_mv | 10.3760/cma.j.issn.0376-2491.2012.09.018 |
| format | Article |
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The model of TLE was induced by lithium-pilocarpine in the experiment group. And the rats were further divided into the STLE and non-STLE groups based upon the absence or presence of recurrent spontaneous seizure in the next 30 days. Total protein fractions from CA1 area of hippocampus were successively obtained through tissue homogenates abstraction. The HSP27 expression in the CA1 area of hippocamp from three groups was semi-quantitatively analyzed by Western blot. And the expression of HSP27 in CA1 area was detected by pre-embedding immunogold electron microscopy.
Expression of HSP27 in the hippocampus CA1 area as detected by Western blot was in accord with that by immunogold electron microscopy. Relative optical density values were 0.912 ± 0.011, 0.431 ± 0.011 and 0.428 ± 0.010 respectively. And gold particle</description><identifier>ISSN: 0376-2491</identifier><identifier>DOI: 10.3760/cma.j.issn.0376-2491.2012.09.018</identifier><identifier>PMID: 22800957</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Blotting, Western ; CA1 Region, Hippocampal - metabolism ; Disease Models, Animal ; Epilepsy, Temporal Lobe - metabolism ; HSP27 Heat-Shock Proteins - metabolism ; Male ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Zhong hua yi xue za zhi, 2012-03, Vol.92 (9), p.642-644</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22800957$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Pu</creatorcontrib><creatorcontrib>Wu, Liu-yi-wen</creatorcontrib><creatorcontrib>Xiao, Bo</creatorcontrib><creatorcontrib>Chen, Ying-zhu</creatorcontrib><creatorcontrib>Wu, Xiao-mei</creatorcontrib><creatorcontrib>Huang, Zhi-ling</creatorcontrib><title>Expression of HSP27 in CA1 area of hippocampus in temporal lobe epilepsy model</title><title>Zhong hua yi xue za zhi</title><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><description>To explore the expression of heat shock protein 27 (HSP27) in the CA1 area of hippocampus in temporal lobe epilepsy (TLE) so as to elucidate the relationship between HSP27 and epileptogenesis of TLE.
The model of TLE was induced by lithium-pilocarpine in the experiment group. And the rats were further divided into the STLE and non-STLE groups based upon the absence or presence of recurrent spontaneous seizure in the next 30 days. Total protein fractions from CA1 area of hippocampus were successively obtained through tissue homogenates abstraction. The HSP27 expression in the CA1 area of hippocamp from three groups was semi-quantitatively analyzed by Western blot. And the expression of HSP27 in CA1 area was detected by pre-embedding immunogold electron microscopy.
Expression of HSP27 in the hippocampus CA1 area as detected by Western blot was in accord with that by immunogold electron microscopy. Relative optical density values were 0.912 ± 0.011, 0.431 ± 0.011 and 0.428 ± 0.010 respectively. And gold particle</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>CA1 Region, Hippocampal - metabolism</subject><subject>Disease Models, Animal</subject><subject>Epilepsy, Temporal Lobe - metabolism</subject><subject>HSP27 Heat-Shock Proteins - metabolism</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0376-2491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kFFLwzAUhfOguDH3FySPe2lNbtqkfRxjOmGooD6XZLnFStLEZgX37604fTrwncPlnkPIirNcKMluD17nH3mXUp-zCWRQ1DwHxiFndc54dUHm_3xGlil1hhVK1MCAX5EZQMVYXao5edx-xQEnP_Q0tHT38gyKdj3drDnVA-of-N7FGA7axzH9WEf0MQzaURcMUoydw5hO1AeL7ppcttolXJ51Qd7utq-bXbZ_un_YrPdZ5CCPWVG20ydaKbSmRSbQGKUMlwBG2qqEolRWKzk15VIaK3TNCitLVkkJpeZaLMjq924cwueI6dj4Lh3QOd1jGFPDGchKigqKKXpzjo7Go23i0Hk9nJq_DcQ3UmNfqw</recordid><startdate>20120306</startdate><enddate>20120306</enddate><creator>Yang, Pu</creator><creator>Wu, Liu-yi-wen</creator><creator>Xiao, Bo</creator><creator>Chen, Ying-zhu</creator><creator>Wu, Xiao-mei</creator><creator>Huang, Zhi-ling</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20120306</creationdate><title>Expression of HSP27 in CA1 area of hippocampus in temporal lobe epilepsy model</title><author>Yang, Pu ; Wu, Liu-yi-wen ; Xiao, Bo ; Chen, Ying-zhu ; Wu, Xiao-mei ; Huang, Zhi-ling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-45f202a77edbfe03ebb77b1622b6d852457da76760166bd3a904d65086625a1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>CA1 Region, Hippocampal - metabolism</topic><topic>Disease Models, Animal</topic><topic>Epilepsy, Temporal Lobe - metabolism</topic><topic>HSP27 Heat-Shock Proteins - metabolism</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>online_resources</toplevel><creatorcontrib>Yang, Pu</creatorcontrib><creatorcontrib>Wu, Liu-yi-wen</creatorcontrib><creatorcontrib>Xiao, Bo</creatorcontrib><creatorcontrib>Chen, Ying-zhu</creatorcontrib><creatorcontrib>Wu, Xiao-mei</creatorcontrib><creatorcontrib>Huang, Zhi-ling</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhong hua yi xue za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Pu</au><au>Wu, Liu-yi-wen</au><au>Xiao, Bo</au><au>Chen, Ying-zhu</au><au>Wu, Xiao-mei</au><au>Huang, Zhi-ling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of HSP27 in CA1 area of hippocampus in temporal lobe epilepsy model</atitle><jtitle>Zhong hua yi xue za zhi</jtitle><addtitle>Zhonghua Yi Xue Za Zhi</addtitle><date>2012-03-06</date><risdate>2012</risdate><volume>92</volume><issue>9</issue><spage>642</spage><epage>644</epage><pages>642-644</pages><issn>0376-2491</issn><abstract>To explore the expression of heat shock protein 27 (HSP27) in the CA1 area of hippocampus in temporal lobe epilepsy (TLE) so as to elucidate the relationship between HSP27 and epileptogenesis of TLE.
The model of TLE was induced by lithium-pilocarpine in the experiment group. And the rats were further divided into the STLE and non-STLE groups based upon the absence or presence of recurrent spontaneous seizure in the next 30 days. Total protein fractions from CA1 area of hippocampus were successively obtained through tissue homogenates abstraction. The HSP27 expression in the CA1 area of hippocamp from three groups was semi-quantitatively analyzed by Western blot. And the expression of HSP27 in CA1 area was detected by pre-embedding immunogold electron microscopy.
Expression of HSP27 in the hippocampus CA1 area as detected by Western blot was in accord with that by immunogold electron microscopy. Relative optical density values were 0.912 ± 0.011, 0.431 ± 0.011 and 0.428 ± 0.010 respectively. And gold particle</abstract><cop>China</cop><pmid>22800957</pmid><doi>10.3760/cma.j.issn.0376-2491.2012.09.018</doi><tpages>3</tpages></addata></record> |
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| language | chi |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Blotting, Western CA1 Region, Hippocampal - metabolism Disease Models, Animal Epilepsy, Temporal Lobe - metabolism HSP27 Heat-Shock Proteins - metabolism Male Rats Rats, Sprague-Dawley |
| title | Expression of HSP27 in CA1 area of hippocampus in temporal lobe epilepsy model |
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