27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells
The principal aim of this study was to analyze in estrogen receptor-positive MCF7 cells the response of three estrogen-dependent proteins to 27-hydroxycholesterol (27OHC), a major circulating cholesterol metabolite. Immunofluorescence, immunoblotting and immunogold labelling analyses of MCF7 cells e...
Gespeichert in:
| Veröffentlicht in: | Oncology reports 2012-09, Vol.28 (3), p.992-998 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 998 |
|---|---|
| container_issue | 3 |
| container_start_page | 992 |
| container_title | Oncology reports |
| container_volume | 28 |
| creator | CRUZ, PAMELA EPUÑÁN, MARÍA JOSÉ RAMÍREZ, MARÍA EUGENIA TORRES, CRISTIAN G VALLADARES, LUIS E SIERRALTA, WALTER D |
| description | The principal aim of this study was to analyze in estrogen receptor-positive MCF7 cells the response of three estrogen-dependent proteins to 27-hydroxycholesterol (27OHC), a major circulating cholesterol metabolite. Immunofluorescence, immunoblotting and immunogold labelling analyses of MCF7 cells exposed for up to 72 h to 2 nM estradiol (E2) or to 2 μM 27OHC demonstrated similar responses in the expression of MnSOD and ERβ compared to the non-stimulated cells. Thus, the results confirm 27OHC's function as a novel selective estrogen receptor modulator (SERM). The epithelial to mesenchymal transition (EMT), observed in MCF7 cells stimulated for longer than 48 h with 2 μM 27OHC, was accompanied by lower immunoreactive levels of nuclear FOXM1 in comparison to E2-treated cells. The results presented in this study are discussed taking into consideration the relationship of hypercholesterolemia, 27OHC production, ROS synthesis and macrophage infiltration, potentially occurring in obese patients with ERα-positive, infiltrated mammary tumors. |
| doi_str_mv | 10.3892/or.2012.1859 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1026689983</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1932671594</sourcerecordid><originalsourceid>FETCH-LOGICAL-c484t-5531d1e30946eb4eb68a9e943cb067dd498a7570e573a9edde8869582c63954f3</originalsourceid><addsrcrecordid>eNpdkU2LUzEUhoMozljduZYLorgwNd8fy6E4Koy4URBcXNKbc22G26Tm3Mr035vS6oCrhHMe3rw8IeQ5Z0vpvHhX6lIwLpbcaf-AXHLrORVK8oftzgSnUurvF-QJ4i1jwjLjH5MLISxnXrlL8kNYujnEWu4Ow6ZMgDPUMnUhx27eQAd3uwqIqeSujG1Soc1wruUnZIqQMc3pN3S7WmZIGbuUu8-ra9sNME34lDwaw4Tw7HwuyLfr919XH-nNlw-fVlc3dFBOzVRrySMH2QoZWCtYGxc8eCWHNTM2RuVdsNoy0Fa2RYzgnPHaicFIr9UoF-TNKbfV-LVv9fptwmODkKHssW8ajHHeO9nQl_-ht2Vfc2vXcy-FsVy3hxfk7YkaakGsMPa7mrahHlpUf5Tel9ofpfdH6Q1_cQ7dr7cQ_8F_LTfg1RkIOIRprCEPCe8502IUs417feJw1z4gxXLPlEqFo0xS5r2QfwDQpZV8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1932671594</pqid></control><display><type>article</type><title>27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>CRUZ, PAMELA ; EPUÑÁN, MARÍA JOSÉ ; RAMÍREZ, MARÍA EUGENIA ; TORRES, CRISTIAN G ; VALLADARES, LUIS E ; SIERRALTA, WALTER D</creator><creatorcontrib>CRUZ, PAMELA ; EPUÑÁN, MARÍA JOSÉ ; RAMÍREZ, MARÍA EUGENIA ; TORRES, CRISTIAN G ; VALLADARES, LUIS E ; SIERRALTA, WALTER D</creatorcontrib><description>The principal aim of this study was to analyze in estrogen receptor-positive MCF7 cells the response of three estrogen-dependent proteins to 27-hydroxycholesterol (27OHC), a major circulating cholesterol metabolite. Immunofluorescence, immunoblotting and immunogold labelling analyses of MCF7 cells exposed for up to 72 h to 2 nM estradiol (E2) or to 2 μM 27OHC demonstrated similar responses in the expression of MnSOD and ERβ compared to the non-stimulated cells. Thus, the results confirm 27OHC's function as a novel selective estrogen receptor modulator (SERM). The epithelial to mesenchymal transition (EMT), observed in MCF7 cells stimulated for longer than 48 h with 2 μM 27OHC, was accompanied by lower immunoreactive levels of nuclear FOXM1 in comparison to E2-treated cells. The results presented in this study are discussed taking into consideration the relationship of hypercholesterolemia, 27OHC production, ROS synthesis and macrophage infiltration, potentially occurring in obese patients with ERα-positive, infiltrated mammary tumors.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2012.1859</identifier><identifier>PMID: 22710948</identifier><language>eng</language><publisher>Athens: D.A. Spandidos</publisher><subject>27-hydroxycholesterol ; Biological and medical sciences ; Breast cancer ; Cell Line, Tumor ; EGFR2/neu ; Epithelial-Mesenchymal Transition ; ERβ ; Estradiol - pharmacology ; Estradiol - physiology ; Estrogen Receptor alpha - metabolism ; Estrogen Receptor beta - metabolism ; Estrogens ; Estrogens - pharmacology ; Estrogens - physiology ; Forkhead Box Protein M1 ; Forkhead Transcription Factors - metabolism ; FOXM1 ; Gynecology. Andrology. Obstetrics ; Humans ; Hydroxycholesterols - pharmacology ; Kinases ; mammary cancer ; Mammary gland diseases ; MCF7 cells ; Medical sciences ; Mitochondria - enzymology ; Mitochondria - metabolism ; Mitochondria - ultrastructure ; MnSOD ; Reactive Oxygen Species - metabolism ; Receptors, Estrogen - metabolism ; Rodents ; Superoxide Dismutase - genetics ; Superoxide Dismutase - metabolism ; Tumors</subject><ispartof>Oncology reports, 2012-09, Vol.28 (3), p.992-998</ispartof><rights>Copyright © 2012, Spandidos Publications</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Spandidos Publications UK Ltd. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-5531d1e30946eb4eb68a9e943cb067dd498a7570e573a9edde8869582c63954f3</citedby><cites>FETCH-LOGICAL-c484t-5531d1e30946eb4eb68a9e943cb067dd498a7570e573a9edde8869582c63954f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26185407$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22710948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CRUZ, PAMELA</creatorcontrib><creatorcontrib>EPUÑÁN, MARÍA JOSÉ</creatorcontrib><creatorcontrib>RAMÍREZ, MARÍA EUGENIA</creatorcontrib><creatorcontrib>TORRES, CRISTIAN G</creatorcontrib><creatorcontrib>VALLADARES, LUIS E</creatorcontrib><creatorcontrib>SIERRALTA, WALTER D</creatorcontrib><title>27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>The principal aim of this study was to analyze in estrogen receptor-positive MCF7 cells the response of three estrogen-dependent proteins to 27-hydroxycholesterol (27OHC), a major circulating cholesterol metabolite. Immunofluorescence, immunoblotting and immunogold labelling analyses of MCF7 cells exposed for up to 72 h to 2 nM estradiol (E2) or to 2 μM 27OHC demonstrated similar responses in the expression of MnSOD and ERβ compared to the non-stimulated cells. Thus, the results confirm 27OHC's function as a novel selective estrogen receptor modulator (SERM). The epithelial to mesenchymal transition (EMT), observed in MCF7 cells stimulated for longer than 48 h with 2 μM 27OHC, was accompanied by lower immunoreactive levels of nuclear FOXM1 in comparison to E2-treated cells. The results presented in this study are discussed taking into consideration the relationship of hypercholesterolemia, 27OHC production, ROS synthesis and macrophage infiltration, potentially occurring in obese patients with ERα-positive, infiltrated mammary tumors.</description><subject>27-hydroxycholesterol</subject><subject>Biological and medical sciences</subject><subject>Breast cancer</subject><subject>Cell Line, Tumor</subject><subject>EGFR2/neu</subject><subject>Epithelial-Mesenchymal Transition</subject><subject>ERβ</subject><subject>Estradiol - pharmacology</subject><subject>Estradiol - physiology</subject><subject>Estrogen Receptor alpha - metabolism</subject><subject>Estrogen Receptor beta - metabolism</subject><subject>Estrogens</subject><subject>Estrogens - pharmacology</subject><subject>Estrogens - physiology</subject><subject>Forkhead Box Protein M1</subject><subject>Forkhead Transcription Factors - metabolism</subject><subject>FOXM1</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Hydroxycholesterols - pharmacology</subject><subject>Kinases</subject><subject>mammary cancer</subject><subject>Mammary gland diseases</subject><subject>MCF7 cells</subject><subject>Medical sciences</subject><subject>Mitochondria - enzymology</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria - ultrastructure</subject><subject>MnSOD</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Receptors, Estrogen - metabolism</subject><subject>Rodents</subject><subject>Superoxide Dismutase - genetics</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Tumors</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkU2LUzEUhoMozljduZYLorgwNd8fy6E4Koy4URBcXNKbc22G26Tm3Mr035vS6oCrhHMe3rw8IeQ5Z0vpvHhX6lIwLpbcaf-AXHLrORVK8oftzgSnUurvF-QJ4i1jwjLjH5MLISxnXrlL8kNYujnEWu4Ow6ZMgDPUMnUhx27eQAd3uwqIqeSujG1Soc1wruUnZIqQMc3pN3S7WmZIGbuUu8-ra9sNME34lDwaw4Tw7HwuyLfr919XH-nNlw-fVlc3dFBOzVRrySMH2QoZWCtYGxc8eCWHNTM2RuVdsNoy0Fa2RYzgnPHaicFIr9UoF-TNKbfV-LVv9fptwmODkKHssW8ajHHeO9nQl_-ht2Vfc2vXcy-FsVy3hxfk7YkaakGsMPa7mrahHlpUf5Tel9ofpfdH6Q1_cQ7dr7cQ_8F_LTfg1RkIOIRprCEPCe8502IUs417feJw1z4gxXLPlEqFo0xS5r2QfwDQpZV8</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>CRUZ, PAMELA</creator><creator>EPUÑÁN, MARÍA JOSÉ</creator><creator>RAMÍREZ, MARÍA EUGENIA</creator><creator>TORRES, CRISTIAN G</creator><creator>VALLADARES, LUIS E</creator><creator>SIERRALTA, WALTER D</creator><general>D.A. Spandidos</general><general>Spandidos</general><general>Spandidos Publications UK Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20120901</creationdate><title>27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells</title><author>CRUZ, PAMELA ; EPUÑÁN, MARÍA JOSÉ ; RAMÍREZ, MARÍA EUGENIA ; TORRES, CRISTIAN G ; VALLADARES, LUIS E ; SIERRALTA, WALTER D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-5531d1e30946eb4eb68a9e943cb067dd498a7570e573a9edde8869582c63954f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>27-hydroxycholesterol</topic><topic>Biological and medical sciences</topic><topic>Breast cancer</topic><topic>Cell Line, Tumor</topic><topic>EGFR2/neu</topic><topic>Epithelial-Mesenchymal Transition</topic><topic>ERβ</topic><topic>Estradiol - pharmacology</topic><topic>Estradiol - physiology</topic><topic>Estrogen Receptor alpha - metabolism</topic><topic>Estrogen Receptor beta - metabolism</topic><topic>Estrogens</topic><topic>Estrogens - pharmacology</topic><topic>Estrogens - physiology</topic><topic>Forkhead Box Protein M1</topic><topic>Forkhead Transcription Factors - metabolism</topic><topic>FOXM1</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Hydroxycholesterols - pharmacology</topic><topic>Kinases</topic><topic>mammary cancer</topic><topic>Mammary gland diseases</topic><topic>MCF7 cells</topic><topic>Medical sciences</topic><topic>Mitochondria - enzymology</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondria - ultrastructure</topic><topic>MnSOD</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Receptors, Estrogen - metabolism</topic><topic>Rodents</topic><topic>Superoxide Dismutase - genetics</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CRUZ, PAMELA</creatorcontrib><creatorcontrib>EPUÑÁN, MARÍA JOSÉ</creatorcontrib><creatorcontrib>RAMÍREZ, MARÍA EUGENIA</creatorcontrib><creatorcontrib>TORRES, CRISTIAN G</creatorcontrib><creatorcontrib>VALLADARES, LUIS E</creatorcontrib><creatorcontrib>SIERRALTA, WALTER D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CRUZ, PAMELA</au><au>EPUÑÁN, MARÍA JOSÉ</au><au>RAMÍREZ, MARÍA EUGENIA</au><au>TORRES, CRISTIAN G</au><au>VALLADARES, LUIS E</au><au>SIERRALTA, WALTER D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>28</volume><issue>3</issue><spage>992</spage><epage>998</epage><pages>992-998</pages><issn>1021-335X</issn><eissn>1791-2431</eissn><abstract>The principal aim of this study was to analyze in estrogen receptor-positive MCF7 cells the response of three estrogen-dependent proteins to 27-hydroxycholesterol (27OHC), a major circulating cholesterol metabolite. Immunofluorescence, immunoblotting and immunogold labelling analyses of MCF7 cells exposed for up to 72 h to 2 nM estradiol (E2) or to 2 μM 27OHC demonstrated similar responses in the expression of MnSOD and ERβ compared to the non-stimulated cells. Thus, the results confirm 27OHC's function as a novel selective estrogen receptor modulator (SERM). The epithelial to mesenchymal transition (EMT), observed in MCF7 cells stimulated for longer than 48 h with 2 μM 27OHC, was accompanied by lower immunoreactive levels of nuclear FOXM1 in comparison to E2-treated cells. The results presented in this study are discussed taking into consideration the relationship of hypercholesterolemia, 27OHC production, ROS synthesis and macrophage infiltration, potentially occurring in obese patients with ERα-positive, infiltrated mammary tumors.</abstract><cop>Athens</cop><pub>D.A. Spandidos</pub><pmid>22710948</pmid><doi>10.3892/or.2012.1859</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1021-335X |
| ispartof | Oncology reports, 2012-09, Vol.28 (3), p.992-998 |
| issn | 1021-335X 1791-2431 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1026689983 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 27-hydroxycholesterol Biological and medical sciences Breast cancer Cell Line, Tumor EGFR2/neu Epithelial-Mesenchymal Transition ERβ Estradiol - pharmacology Estradiol - physiology Estrogen Receptor alpha - metabolism Estrogen Receptor beta - metabolism Estrogens Estrogens - pharmacology Estrogens - physiology Forkhead Box Protein M1 Forkhead Transcription Factors - metabolism FOXM1 Gynecology. Andrology. Obstetrics Humans Hydroxycholesterols - pharmacology Kinases mammary cancer Mammary gland diseases MCF7 cells Medical sciences Mitochondria - enzymology Mitochondria - metabolism Mitochondria - ultrastructure MnSOD Reactive Oxygen Species - metabolism Receptors, Estrogen - metabolism Rodents Superoxide Dismutase - genetics Superoxide Dismutase - metabolism Tumors |
| title | 27-hydroxycholesterol and the expression of three estrogen-sensitive proteins in MCF7 cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T18%3A29%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=27-hydroxycholesterol%20and%20the%20expression%20of%20three%20estrogen-sensitive%20proteins%20in%20MCF7%20cells&rft.jtitle=Oncology%20reports&rft.au=CRUZ,%20PAMELA&rft.date=2012-09-01&rft.volume=28&rft.issue=3&rft.spage=992&rft.epage=998&rft.pages=992-998&rft.issn=1021-335X&rft.eissn=1791-2431&rft_id=info:doi/10.3892/or.2012.1859&rft_dat=%3Cproquest_cross%3E1932671594%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1932671594&rft_id=info:pmid/22710948&rfr_iscdi=true |