Determination of Cryoprotectant for Magnetic Cryopreservation of Dental Pulp Tissue
Dental pulp, covered with dental hard tissue, is a promising source of mesenchymal stem cells and osteoprogenitor cells for regenerative medicine. Our previous studies showed that 73% of dental pulp cells isolated from magnetically cryopreserved teeth where their viability, morphology, and expressio...
Gespeichert in:
| Veröffentlicht in: | Tissue engineering. Part C, Methods Methods, 2012-06, Vol.18 (6), p.397-407 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 407 |
|---|---|
| container_issue | 6 |
| container_start_page | 397 |
| container_title | Tissue engineering. Part C, Methods |
| container_volume | 18 |
| creator | Lee, Sheng-Yang Sean Sun, Chao-Hsuan Benson Kuo, Tzong-Fu Huang, Yen-Hua Jeng, Jiiang-Huei Yang, Jen-Chang Yang, Wei-Chung Vivian |
| description | Dental pulp, covered with dental hard tissue, is a promising source of mesenchymal stem cells and osteoprogenitor cells for regenerative medicine. Our previous studies showed that 73% of dental pulp cells isolated from magnetically cryopreserved teeth where their viability, morphology, and expression of stem cell surface markers were similar to the cells isolated from fresh teeth, suggesting that magnetic cryopreservation is an applicable method for intact tooth as well as dental pulp tissue banking. However, the cryoprotectant, concentration, contact surface, and equilibration time for magnetic cryopreservation of dental pulp require optimization. In addition, the integrity and viability of post-thawed dental pulp with and without dental hard tissue covering after magnetic cryopreservation were investigated. Lower concentration of the cryoprotectant (5% dimethyl sulfoxide [DMSO]) and shorter preequilibration time are required for magnetic cryopreservation compared with the conventional cryopreservation method. The structure of at least 33% of post-thawed pulp with dental hard tissue from the open end remained intact where >80% of cells were viable. The addition of the cryoprotectant additive trehalose did not replace or improve DMSO's efficacy for magnetic cryopreservation of dental pulp or intact tooth. Tooth banking for transplantation provides an alternative treatment to replace missing teeth. The optimized cryoprotectant conditions for dental pulp tissue during magnetic cryopreservation should lead to more satisfactory outcomes in clinical applications such as autotransplantation and the isolation and expansion of dental pulp stem cells for tissue repair. |
| doi_str_mv | 10.1089/ten.tec.2011.0363 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1024666458</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1024666458</sourcerecordid><originalsourceid>FETCH-LOGICAL-c476t-a7f9eb1c3abdabb16fb6867cdba52de201c498d46391baec0df6a095aa5d3f63</originalsourceid><addsrcrecordid>eNqNkc9LwzAUx4MoTqd_gBcpePHSmjRt2hxl8xdMFNy9JOmLdLTpTFJh_72pmzt42iEkvHzel_f4IHRFcEJwye88mMSDSlJMSIIpo0fojHBaxJTy9Hj_LrMJOnduhTHDrOCnaJKmhDJc0jP0MQcPtmuM8E1vol5HM7vp17YPuV4YH-neRq_i04Bv1O4PHNjvPT8H40UbvQ_tOlo2zg1wgU60aB1c7u4pWj4-LGfP8eLt6WV2v4hVVjAfi0JzkERRIWshJWFaspIVqpYiT2sIS6mMl3XGKCdSgMK1ZgLzXIi8pprRKbrdxoZpvwZwvuoap6BthYF-cBXBacYYy_LyAJSUjKaBDejNP3TVD9aEPX4pHEbkNFBkSynbO2dBV2vbdMJuAlSNbqrgJhxVjW6q0U3oud4lD7KDet_xJyMAxRYYy8KYtgEJ1h8Q_QOpCZ9w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1018068693</pqid></control><display><type>article</type><title>Determination of Cryoprotectant for Magnetic Cryopreservation of Dental Pulp Tissue</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Lee, Sheng-Yang Sean ; Sun, Chao-Hsuan Benson ; Kuo, Tzong-Fu ; Huang, Yen-Hua ; Jeng, Jiiang-Huei ; Yang, Jen-Chang ; Yang, Wei-Chung Vivian</creator><creatorcontrib>Lee, Sheng-Yang Sean ; Sun, Chao-Hsuan Benson ; Kuo, Tzong-Fu ; Huang, Yen-Hua ; Jeng, Jiiang-Huei ; Yang, Jen-Chang ; Yang, Wei-Chung Vivian</creatorcontrib><description>Dental pulp, covered with dental hard tissue, is a promising source of mesenchymal stem cells and osteoprogenitor cells for regenerative medicine. Our previous studies showed that 73% of dental pulp cells isolated from magnetically cryopreserved teeth where their viability, morphology, and expression of stem cell surface markers were similar to the cells isolated from fresh teeth, suggesting that magnetic cryopreservation is an applicable method for intact tooth as well as dental pulp tissue banking. However, the cryoprotectant, concentration, contact surface, and equilibration time for magnetic cryopreservation of dental pulp require optimization. In addition, the integrity and viability of post-thawed dental pulp with and without dental hard tissue covering after magnetic cryopreservation were investigated. Lower concentration of the cryoprotectant (5% dimethyl sulfoxide [DMSO]) and shorter preequilibration time are required for magnetic cryopreservation compared with the conventional cryopreservation method. The structure of at least 33% of post-thawed pulp with dental hard tissue from the open end remained intact where >80% of cells were viable. The addition of the cryoprotectant additive trehalose did not replace or improve DMSO's efficacy for magnetic cryopreservation of dental pulp or intact tooth. Tooth banking for transplantation provides an alternative treatment to replace missing teeth. The optimized cryoprotectant conditions for dental pulp tissue during magnetic cryopreservation should lead to more satisfactory outcomes in clinical applications such as autotransplantation and the isolation and expansion of dental pulp stem cells for tissue repair.</description><identifier>ISSN: 1937-3384</identifier><identifier>EISSN: 1937-3392</identifier><identifier>DOI: 10.1089/ten.tec.2011.0363</identifier><identifier>PMID: 22136083</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Cell Adhesion - drug effects ; Cell Shape - drug effects ; Cryogenic engineering ; Cryopreservation ; Cryopreservation - methods ; Cryoprotective Agents - pharmacology ; Cryoprotectors ; Cryotherapy ; Cytology ; Dental pulp ; Dental Pulp - cytology ; Dental Pulp - drug effects ; Dentistry ; Dimethyl sulfoxide ; Freezing ; Incisor - cytology ; Incisor - drug effects ; Magnetics - methods ; Male ; Mesenchyme ; Osteoprogenitor cells ; Rats ; Rats, Wistar ; Regeneration ; Stem cells ; Surface markers ; Teeth ; Therapeutic applications ; Tissue engineering ; Tissue Survival - drug effects ; Transplants & implants ; Trehalose ; Trehalose - pharmacology</subject><ispartof>Tissue engineering. Part C, Methods, 2012-06, Vol.18 (6), p.397-407</ispartof><rights>2012, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2012, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-a7f9eb1c3abdabb16fb6867cdba52de201c498d46391baec0df6a095aa5d3f63</citedby><cites>FETCH-LOGICAL-c476t-a7f9eb1c3abdabb16fb6867cdba52de201c498d46391baec0df6a095aa5d3f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22136083$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Sheng-Yang Sean</creatorcontrib><creatorcontrib>Sun, Chao-Hsuan Benson</creatorcontrib><creatorcontrib>Kuo, Tzong-Fu</creatorcontrib><creatorcontrib>Huang, Yen-Hua</creatorcontrib><creatorcontrib>Jeng, Jiiang-Huei</creatorcontrib><creatorcontrib>Yang, Jen-Chang</creatorcontrib><creatorcontrib>Yang, Wei-Chung Vivian</creatorcontrib><title>Determination of Cryoprotectant for Magnetic Cryopreservation of Dental Pulp Tissue</title><title>Tissue engineering. Part C, Methods</title><addtitle>Tissue Eng Part C Methods</addtitle><description>Dental pulp, covered with dental hard tissue, is a promising source of mesenchymal stem cells and osteoprogenitor cells for regenerative medicine. Our previous studies showed that 73% of dental pulp cells isolated from magnetically cryopreserved teeth where their viability, morphology, and expression of stem cell surface markers were similar to the cells isolated from fresh teeth, suggesting that magnetic cryopreservation is an applicable method for intact tooth as well as dental pulp tissue banking. However, the cryoprotectant, concentration, contact surface, and equilibration time for magnetic cryopreservation of dental pulp require optimization. In addition, the integrity and viability of post-thawed dental pulp with and without dental hard tissue covering after magnetic cryopreservation were investigated. Lower concentration of the cryoprotectant (5% dimethyl sulfoxide [DMSO]) and shorter preequilibration time are required for magnetic cryopreservation compared with the conventional cryopreservation method. The structure of at least 33% of post-thawed pulp with dental hard tissue from the open end remained intact where >80% of cells were viable. The addition of the cryoprotectant additive trehalose did not replace or improve DMSO's efficacy for magnetic cryopreservation of dental pulp or intact tooth. Tooth banking for transplantation provides an alternative treatment to replace missing teeth. The optimized cryoprotectant conditions for dental pulp tissue during magnetic cryopreservation should lead to more satisfactory outcomes in clinical applications such as autotransplantation and the isolation and expansion of dental pulp stem cells for tissue repair.</description><subject>Animals</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Shape - drug effects</subject><subject>Cryogenic engineering</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Cryoprotectors</subject><subject>Cryotherapy</subject><subject>Cytology</subject><subject>Dental pulp</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - drug effects</subject><subject>Dentistry</subject><subject>Dimethyl sulfoxide</subject><subject>Freezing</subject><subject>Incisor - cytology</subject><subject>Incisor - drug effects</subject><subject>Magnetics - methods</subject><subject>Male</subject><subject>Mesenchyme</subject><subject>Osteoprogenitor cells</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Regeneration</subject><subject>Stem cells</subject><subject>Surface markers</subject><subject>Teeth</subject><subject>Therapeutic applications</subject><subject>Tissue engineering</subject><subject>Tissue Survival - drug effects</subject><subject>Transplants & implants</subject><subject>Trehalose</subject><subject>Trehalose - pharmacology</subject><issn>1937-3384</issn><issn>1937-3392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc9LwzAUx4MoTqd_gBcpePHSmjRt2hxl8xdMFNy9JOmLdLTpTFJh_72pmzt42iEkvHzel_f4IHRFcEJwye88mMSDSlJMSIIpo0fojHBaxJTy9Hj_LrMJOnduhTHDrOCnaJKmhDJc0jP0MQcPtmuM8E1vol5HM7vp17YPuV4YH-neRq_i04Bv1O4PHNjvPT8H40UbvQ_tOlo2zg1wgU60aB1c7u4pWj4-LGfP8eLt6WV2v4hVVjAfi0JzkERRIWshJWFaspIVqpYiT2sIS6mMl3XGKCdSgMK1ZgLzXIi8pprRKbrdxoZpvwZwvuoap6BthYF-cBXBacYYy_LyAJSUjKaBDejNP3TVD9aEPX4pHEbkNFBkSynbO2dBV2vbdMJuAlSNbqrgJhxVjW6q0U3oud4lD7KDet_xJyMAxRYYy8KYtgEJ1h8Q_QOpCZ9w</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Lee, Sheng-Yang Sean</creator><creator>Sun, Chao-Hsuan Benson</creator><creator>Kuo, Tzong-Fu</creator><creator>Huang, Yen-Hua</creator><creator>Jeng, Jiiang-Huei</creator><creator>Yang, Jen-Chang</creator><creator>Yang, Wei-Chung Vivian</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20120601</creationdate><title>Determination of Cryoprotectant for Magnetic Cryopreservation of Dental Pulp Tissue</title><author>Lee, Sheng-Yang Sean ; Sun, Chao-Hsuan Benson ; Kuo, Tzong-Fu ; Huang, Yen-Hua ; Jeng, Jiiang-Huei ; Yang, Jen-Chang ; Yang, Wei-Chung Vivian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-a7f9eb1c3abdabb16fb6867cdba52de201c498d46391baec0df6a095aa5d3f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Shape - drug effects</topic><topic>Cryogenic engineering</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Cryoprotectors</topic><topic>Cryotherapy</topic><topic>Cytology</topic><topic>Dental pulp</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - drug effects</topic><topic>Dentistry</topic><topic>Dimethyl sulfoxide</topic><topic>Freezing</topic><topic>Incisor - cytology</topic><topic>Incisor - drug effects</topic><topic>Magnetics - methods</topic><topic>Male</topic><topic>Mesenchyme</topic><topic>Osteoprogenitor cells</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Regeneration</topic><topic>Stem cells</topic><topic>Surface markers</topic><topic>Teeth</topic><topic>Therapeutic applications</topic><topic>Tissue engineering</topic><topic>Tissue Survival - drug effects</topic><topic>Transplants & implants</topic><topic>Trehalose</topic><topic>Trehalose - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Sheng-Yang Sean</creatorcontrib><creatorcontrib>Sun, Chao-Hsuan Benson</creatorcontrib><creatorcontrib>Kuo, Tzong-Fu</creatorcontrib><creatorcontrib>Huang, Yen-Hua</creatorcontrib><creatorcontrib>Jeng, Jiiang-Huei</creatorcontrib><creatorcontrib>Yang, Jen-Chang</creatorcontrib><creatorcontrib>Yang, Wei-Chung Vivian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Tissue engineering. Part C, Methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Sheng-Yang Sean</au><au>Sun, Chao-Hsuan Benson</au><au>Kuo, Tzong-Fu</au><au>Huang, Yen-Hua</au><au>Jeng, Jiiang-Huei</au><au>Yang, Jen-Chang</au><au>Yang, Wei-Chung Vivian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Cryoprotectant for Magnetic Cryopreservation of Dental Pulp Tissue</atitle><jtitle>Tissue engineering. Part C, Methods</jtitle><addtitle>Tissue Eng Part C Methods</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>18</volume><issue>6</issue><spage>397</spage><epage>407</epage><pages>397-407</pages><issn>1937-3384</issn><eissn>1937-3392</eissn><abstract>Dental pulp, covered with dental hard tissue, is a promising source of mesenchymal stem cells and osteoprogenitor cells for regenerative medicine. Our previous studies showed that 73% of dental pulp cells isolated from magnetically cryopreserved teeth where their viability, morphology, and expression of stem cell surface markers were similar to the cells isolated from fresh teeth, suggesting that magnetic cryopreservation is an applicable method for intact tooth as well as dental pulp tissue banking. However, the cryoprotectant, concentration, contact surface, and equilibration time for magnetic cryopreservation of dental pulp require optimization. In addition, the integrity and viability of post-thawed dental pulp with and without dental hard tissue covering after magnetic cryopreservation were investigated. Lower concentration of the cryoprotectant (5% dimethyl sulfoxide [DMSO]) and shorter preequilibration time are required for magnetic cryopreservation compared with the conventional cryopreservation method. The structure of at least 33% of post-thawed pulp with dental hard tissue from the open end remained intact where >80% of cells were viable. The addition of the cryoprotectant additive trehalose did not replace or improve DMSO's efficacy for magnetic cryopreservation of dental pulp or intact tooth. Tooth banking for transplantation provides an alternative treatment to replace missing teeth. The optimized cryoprotectant conditions for dental pulp tissue during magnetic cryopreservation should lead to more satisfactory outcomes in clinical applications such as autotransplantation and the isolation and expansion of dental pulp stem cells for tissue repair.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>22136083</pmid><doi>10.1089/ten.tec.2011.0363</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1937-3384 |
| ispartof | Tissue engineering. Part C, Methods, 2012-06, Vol.18 (6), p.397-407 |
| issn | 1937-3384 1937-3392 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1024666458 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Adhesion - drug effects Cell Shape - drug effects Cryogenic engineering Cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacology Cryoprotectors Cryotherapy Cytology Dental pulp Dental Pulp - cytology Dental Pulp - drug effects Dentistry Dimethyl sulfoxide Freezing Incisor - cytology Incisor - drug effects Magnetics - methods Male Mesenchyme Osteoprogenitor cells Rats Rats, Wistar Regeneration Stem cells Surface markers Teeth Therapeutic applications Tissue engineering Tissue Survival - drug effects Transplants & implants Trehalose Trehalose - pharmacology |
| title | Determination of Cryoprotectant for Magnetic Cryopreservation of Dental Pulp Tissue |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T15%3A11%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20Cryoprotectant%20for%20Magnetic%20Cryopreservation%20of%20Dental%20Pulp%20Tissue&rft.jtitle=Tissue%20engineering.%20Part%20C,%20Methods&rft.au=Lee,%20Sheng-Yang%20Sean&rft.date=2012-06-01&rft.volume=18&rft.issue=6&rft.spage=397&rft.epage=407&rft.pages=397-407&rft.issn=1937-3384&rft.eissn=1937-3392&rft_id=info:doi/10.1089/ten.tec.2011.0363&rft_dat=%3Cproquest_cross%3E1024666458%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1018068693&rft_id=info:pmid/22136083&rfr_iscdi=true |