Chromatin intact human sperm recovery is higher following glass wool column filtration as compared with density gradient centrifugation
Summary The sperm DNA quality as determined by chromatin integrity has been reported to be associated with in vivo and in vitro fertility. However, previous studies have evaluated preparation procedures to select motile, morphologically normal and mature spermatozoa, but not the spermatozoa with int...
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| Veröffentlicht in: | Andrologia 2012-05, Vol.44 (s1), p.248-251 |
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| creator | Sauer, R. Coulam, C. B. Jeyendran, R. S. |
| description | Summary
The sperm DNA quality as determined by chromatin integrity has been reported to be associated with in vivo and in vitro fertility. However, previous studies have evaluated preparation procedures to select motile, morphologically normal and mature spermatozoa, but not the spermatozoa with intact sperm chromatin. To determine which technique yields a population of spermatozoa with improved DNA quality, split ejaculate was processed with density gradient centrifugation (DGC) procedure and glass wool column filtration (GWF) procedure. The processed samples were analysed for sperm DNA quality using the acridine orange staining method on flow cytometry. The GWF procedure decreases the percentage of spermatozoa with DNA fragmentation resulting in more intact chromatin in the processed sample. There is a need to design a clinical study with GWF for assisted reproductive technology. |
| doi_str_mv | 10.1111/j.1439-0272.2011.01171.x |
| format | Article |
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The sperm DNA quality as determined by chromatin integrity has been reported to be associated with in vivo and in vitro fertility. However, previous studies have evaluated preparation procedures to select motile, morphologically normal and mature spermatozoa, but not the spermatozoa with intact sperm chromatin. To determine which technique yields a population of spermatozoa with improved DNA quality, split ejaculate was processed with density gradient centrifugation (DGC) procedure and glass wool column filtration (GWF) procedure. The processed samples were analysed for sperm DNA quality using the acridine orange staining method on flow cytometry. The GWF procedure decreases the percentage of spermatozoa with DNA fragmentation resulting in more intact chromatin in the processed sample. There is a need to design a clinical study with GWF for assisted reproductive technology.</description><identifier>ISSN: 0303-4569</identifier><identifier>EISSN: 1439-0272</identifier><identifier>DOI: 10.1111/j.1439-0272.2011.01171.x</identifier><identifier>PMID: 21689135</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acridine orange ; Centrifugation ; Centrifugation - methods ; Chromatin ; Chromatin - metabolism ; density gradient centrifugation ; Density gradients ; DNA - metabolism ; DNA fragmentation ; Fertility ; Filtration ; Filtration - instrumentation ; Flow Cytometry ; Glass wool ; glass wool column filtration ; Humans ; Male ; Reproduction ; Sperm ; spermatozoa ; Spermatozoa - metabolism</subject><ispartof>Andrologia, 2012-05, Vol.44 (s1), p.248-251</ispartof><rights>2011 Blackwell Verlag GmbH</rights><rights>2011 Blackwell Verlag GmbH.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4411-b2d3f37d6deed3b75717884184d5112836c1e2d61fc7c37326f31befaaa6cb7c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0272.2011.01171.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0272.2011.01171.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,45581,45582</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21689135$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sauer, R.</creatorcontrib><creatorcontrib>Coulam, C. B.</creatorcontrib><creatorcontrib>Jeyendran, R. S.</creatorcontrib><title>Chromatin intact human sperm recovery is higher following glass wool column filtration as compared with density gradient centrifugation</title><title>Andrologia</title><addtitle>Andrologia</addtitle><description>Summary
The sperm DNA quality as determined by chromatin integrity has been reported to be associated with in vivo and in vitro fertility. However, previous studies have evaluated preparation procedures to select motile, morphologically normal and mature spermatozoa, but not the spermatozoa with intact sperm chromatin. To determine which technique yields a population of spermatozoa with improved DNA quality, split ejaculate was processed with density gradient centrifugation (DGC) procedure and glass wool column filtration (GWF) procedure. The processed samples were analysed for sperm DNA quality using the acridine orange staining method on flow cytometry. The GWF procedure decreases the percentage of spermatozoa with DNA fragmentation resulting in more intact chromatin in the processed sample. There is a need to design a clinical study with GWF for assisted reproductive technology.</description><subject>Acridine orange</subject><subject>Centrifugation</subject><subject>Centrifugation - methods</subject><subject>Chromatin</subject><subject>Chromatin - metabolism</subject><subject>density gradient centrifugation</subject><subject>Density gradients</subject><subject>DNA - metabolism</subject><subject>DNA fragmentation</subject><subject>Fertility</subject><subject>Filtration</subject><subject>Filtration - instrumentation</subject><subject>Flow Cytometry</subject><subject>Glass wool</subject><subject>glass wool column filtration</subject><subject>Humans</subject><subject>Male</subject><subject>Reproduction</subject><subject>Sperm</subject><subject>spermatozoa</subject><subject>Spermatozoa - metabolism</subject><issn>0303-4569</issn><issn>1439-0272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhiMEoqvSV0A-cknI2I6dHDhUW1qKlnIBwc1yHCfx4sSLnbC7T8Br1-mWPTPS2KOZ_x9L_pIEQZ5BjPfbDCip0hxznOEcIIvJITu8SFbnwctklZOcpLRg1UVyFcI2j0ELzil9nVxgYGUFpFglf9e9d4OczIjMOEk1oX4e5IjCTvsBea3cH-2PyATUm67XHrXOWrc3Y4c6K0NAe-csUs7Ow4haYycfd7kRyRCbw0563aC9mXrU6DGY6Yg6LxujxwmpeHjTzt2T4U3yqpU26Kvn-zL5fvvx2_pTuvl6d7--3qSKUoC0xg1pCW9Yo3VDal5w4GVJoaRNAYBLwhRo3DBoFVeEE8xaArVupZRM1bF1mbw77d1593vWYRKDCUpbK0ft5iAgx5QVHBf4P6QQ3wNWkSh9-yyd60E3YufNIP1R_PvnKPhwEuyN1cfzHHKxIBVbsZATCzmxIBVPSMVBXD_cLFX0pye_CZM-nP3S_xKME16IHw93gm_K6vbm5xfxmTwCurul7g</recordid><startdate>201205</startdate><enddate>201205</enddate><creator>Sauer, R.</creator><creator>Coulam, C. B.</creator><creator>Jeyendran, R. S.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>201205</creationdate><title>Chromatin intact human sperm recovery is higher following glass wool column filtration as compared with density gradient centrifugation</title><author>Sauer, R. ; Coulam, C. B. ; Jeyendran, R. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4411-b2d3f37d6deed3b75717884184d5112836c1e2d61fc7c37326f31befaaa6cb7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acridine orange</topic><topic>Centrifugation</topic><topic>Centrifugation - methods</topic><topic>Chromatin</topic><topic>Chromatin - metabolism</topic><topic>density gradient centrifugation</topic><topic>Density gradients</topic><topic>DNA - metabolism</topic><topic>DNA fragmentation</topic><topic>Fertility</topic><topic>Filtration</topic><topic>Filtration - instrumentation</topic><topic>Flow Cytometry</topic><topic>Glass wool</topic><topic>glass wool column filtration</topic><topic>Humans</topic><topic>Male</topic><topic>Reproduction</topic><topic>Sperm</topic><topic>spermatozoa</topic><topic>Spermatozoa - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sauer, R.</creatorcontrib><creatorcontrib>Coulam, C. B.</creatorcontrib><creatorcontrib>Jeyendran, R. S.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Andrologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sauer, R.</au><au>Coulam, C. B.</au><au>Jeyendran, R. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromatin intact human sperm recovery is higher following glass wool column filtration as compared with density gradient centrifugation</atitle><jtitle>Andrologia</jtitle><addtitle>Andrologia</addtitle><date>2012-05</date><risdate>2012</risdate><volume>44</volume><issue>s1</issue><spage>248</spage><epage>251</epage><pages>248-251</pages><issn>0303-4569</issn><eissn>1439-0272</eissn><abstract>Summary
The sperm DNA quality as determined by chromatin integrity has been reported to be associated with in vivo and in vitro fertility. However, previous studies have evaluated preparation procedures to select motile, morphologically normal and mature spermatozoa, but not the spermatozoa with intact sperm chromatin. To determine which technique yields a population of spermatozoa with improved DNA quality, split ejaculate was processed with density gradient centrifugation (DGC) procedure and glass wool column filtration (GWF) procedure. The processed samples were analysed for sperm DNA quality using the acridine orange staining method on flow cytometry. The GWF procedure decreases the percentage of spermatozoa with DNA fragmentation resulting in more intact chromatin in the processed sample. There is a need to design a clinical study with GWF for assisted reproductive technology.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21689135</pmid><doi>10.1111/j.1439-0272.2011.01171.x</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Acridine orange Centrifugation Centrifugation - methods Chromatin Chromatin - metabolism density gradient centrifugation Density gradients DNA - metabolism DNA fragmentation Fertility Filtration Filtration - instrumentation Flow Cytometry Glass wool glass wool column filtration Humans Male Reproduction Sperm spermatozoa Spermatozoa - metabolism |
| title | Chromatin intact human sperm recovery is higher following glass wool column filtration as compared with density gradient centrifugation |
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