Dissemination of multidrug-resistant Escherichia coli in Korean veterinary hospitals
Abstract This study was performed to investigate the prevalence of rectal colonization with multidrug-resistant Escherichia coli in dogs hospitalized at veterinary hospitals in Korea and to assess the molecular epidemiologic traits of this organism. A total of 63 unique E. coli isolates obtained fro...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2012-06, Vol.73 (2), p.195-199 |
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| creator | So, Jeong Hwa Kim, Juwon Bae, Il Kwon Jeong, Seok Hoon Kim, So Hyun Lim, Suk-kyung Park, Yong Ho Lee, Kyungwon |
| description | Abstract This study was performed to investigate the prevalence of rectal colonization with multidrug-resistant Escherichia coli in dogs hospitalized at veterinary hospitals in Korea and to assess the molecular epidemiologic traits of this organism. A total of 63 unique E. coli isolates obtained from the rectal swabs of hospitalized dogs were analyzed. Genes encoding CTX-M extended-spectrum β-lactamases (ESBLs) and AmpC enzymes were detected in 21 (33.3%) and 15 (23.8%) canine E. coli isolates, respectively. Twelve canine E. coli isolates harbored both the genes encoding the CTX-M and AmpC enzymes. Six ESBL-producing E. coli isolates also carried the rmtB gene. All 24 E. coli isolates producing CTX-M ESBL and/or CMY-2 were resistant to ciprofloxacin. Furthermore, mutations were found in the gyrA and the parC genes. In most cases, the bla genes of the CTX-M ESBL and AmpC enzymes and the rmtB gene were localized to incompatibility group F (IncF) plasmids. Possible small clonal outbreaks are suggested because some E. coli isolates recovered in the same veterinary hospital were identified as identical sequence types and showed identical banding patterns in repetitive sequence-based polymerase chain reaction. The horizontal transfer of IncF plasmids and the clonal transfer of E. coli strains are suggested to play a role in the dissemination of antimicrobial resistance genes, and this transfer may occur across host species (i.e., between humans and dogs). |
| doi_str_mv | 10.1016/j.diagmicrobio.2012.03.010 |
| format | Article |
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A total of 63 unique E. coli isolates obtained from the rectal swabs of hospitalized dogs were analyzed. Genes encoding CTX-M extended-spectrum β-lactamases (ESBLs) and AmpC enzymes were detected in 21 (33.3%) and 15 (23.8%) canine E. coli isolates, respectively. Twelve canine E. coli isolates harbored both the genes encoding the CTX-M and AmpC enzymes. Six ESBL-producing E. coli isolates also carried the rmtB gene. All 24 E. coli isolates producing CTX-M ESBL and/or CMY-2 were resistant to ciprofloxacin. Furthermore, mutations were found in the gyrA and the parC genes. In most cases, the bla genes of the CTX-M ESBL and AmpC enzymes and the rmtB gene were localized to incompatibility group F (IncF) plasmids. Possible small clonal outbreaks are suggested because some E. coli isolates recovered in the same veterinary hospital were identified as identical sequence types and showed identical banding patterns in repetitive sequence-based polymerase chain reaction. The horizontal transfer of IncF plasmids and the clonal transfer of E. coli strains are suggested to play a role in the dissemination of antimicrobial resistance genes, and this transfer may occur across host species (i.e., between humans and dogs).</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2012.03.010</identifier><identifier>PMID: 22516765</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Bacteriology ; beta-Lactamases - genetics ; Biological and medical sciences ; Carrier State - microbiology ; Carrier State - veterinary ; CMY-2 ; Companion animal ; CTX-M ; DNA, Bacterial - analysis ; Dogs ; Drug Resistance, Multiple, Bacterial ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Escherichia coli Infections - microbiology ; Escherichia coli Infections - veterinary ; Escherichia coli Proteins - genetics ; Fundamental and applied biological sciences. Psychology ; Hospitals, Animal - statistics & numerical data ; IncF plasmid ; Infectious Disease ; Infectious diseases ; Internal Medicine ; Medical sciences ; Microbial Sensitivity Tests ; Microbiology ; Miscellaneous ; Plasmids ; Polymerase Chain Reaction ; Rectum - microbiology ; Republic of Korea ; rmtB</subject><ispartof>Diagnostic microbiology and infectious disease, 2012-06, Vol.73 (2), p.195-199</ispartof><rights>Elsevier Inc.</rights><rights>2012 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c564t-3392b426efb2875a42e4be8cf997b8b0eb32e0a0dd9076a3d79cd7c25579058b3</citedby><cites>FETCH-LOGICAL-c564t-3392b426efb2875a42e4be8cf997b8b0eb32e0a0dd9076a3d79cd7c25579058b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0732889312001022$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26020087$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22516765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>So, Jeong Hwa</creatorcontrib><creatorcontrib>Kim, Juwon</creatorcontrib><creatorcontrib>Bae, Il Kwon</creatorcontrib><creatorcontrib>Jeong, Seok Hoon</creatorcontrib><creatorcontrib>Kim, So Hyun</creatorcontrib><creatorcontrib>Lim, Suk-kyung</creatorcontrib><creatorcontrib>Park, Yong Ho</creatorcontrib><creatorcontrib>Lee, Kyungwon</creatorcontrib><title>Dissemination of multidrug-resistant Escherichia coli in Korean veterinary hospitals</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Abstract This study was performed to investigate the prevalence of rectal colonization with multidrug-resistant Escherichia coli in dogs hospitalized at veterinary hospitals in Korea and to assess the molecular epidemiologic traits of this organism. A total of 63 unique E. coli isolates obtained from the rectal swabs of hospitalized dogs were analyzed. Genes encoding CTX-M extended-spectrum β-lactamases (ESBLs) and AmpC enzymes were detected in 21 (33.3%) and 15 (23.8%) canine E. coli isolates, respectively. Twelve canine E. coli isolates harbored both the genes encoding the CTX-M and AmpC enzymes. Six ESBL-producing E. coli isolates also carried the rmtB gene. All 24 E. coli isolates producing CTX-M ESBL and/or CMY-2 were resistant to ciprofloxacin. Furthermore, mutations were found in the gyrA and the parC genes. In most cases, the bla genes of the CTX-M ESBL and AmpC enzymes and the rmtB gene were localized to incompatibility group F (IncF) plasmids. Possible small clonal outbreaks are suggested because some E. coli isolates recovered in the same veterinary hospital were identified as identical sequence types and showed identical banding patterns in repetitive sequence-based polymerase chain reaction. The horizontal transfer of IncF plasmids and the clonal transfer of E. coli strains are suggested to play a role in the dissemination of antimicrobial resistance genes, and this transfer may occur across host species (i.e., between humans and dogs).</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteriology</subject><subject>beta-Lactamases - genetics</subject><subject>Biological and medical sciences</subject><subject>Carrier State - microbiology</subject><subject>Carrier State - veterinary</subject><subject>CMY-2</subject><subject>Companion animal</subject><subject>CTX-M</subject><subject>DNA, Bacterial - analysis</subject><subject>Dogs</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hospitals, Animal - statistics & numerical data</subject><subject>IncF plasmid</subject><subject>Infectious Disease</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Rectum - microbiology</subject><subject>Republic of Korea</subject><subject>rmtB</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkkFv1DAQhS0EokvhL6AICYlLwthO4oQDUtWWFlGph5az5TiT7iyJvdhJpf57vNotoJ56moO_NzN-bxj7wKHgwOvPm6InczeRDb4jXwjgogBZAIcXbMUb1eYACl6yFSgp8qZp5RF7E-MGEtiW8JodCVHxWtXVit2eUYw4kTMzeZf5IZuWcaY-LHd5wEhxNm7OzqNdYyC7JpNZP1JGLvvhAxqX3eOcXpwJD9naxy3NZoxv2ashFXx3qMfs57fz29PL_Or64vvpyVVuq7qccylb0ZWixqETjapMKbDssLFD26qu6QA7KRAM9H0LqjayV63tlRVVpVqomk4es0_7vtvgfy8YZz1RtDiOxqFfouYgyrpSIOEZKK9E2fCyTOiXPZr8jTHgoLeBpvTBBO24Wm_0_wHoXQAapE4BJPH7w5ylm7D_K310PAEfD4CJ1oxDMM5S_MfVIAAalbizPYfJwHvCoKMldBZ7Cmhn3Xt63j5fn7SxIzlKk3_hA8aNX4JLEWmuY9Lom93J7C6Gpy2SfUL-AcKCv-I</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>So, Jeong Hwa</creator><creator>Kim, Juwon</creator><creator>Bae, Il Kwon</creator><creator>Jeong, Seok Hoon</creator><creator>Kim, So Hyun</creator><creator>Lim, Suk-kyung</creator><creator>Park, Yong Ho</creator><creator>Lee, Kyungwon</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20120601</creationdate><title>Dissemination of multidrug-resistant Escherichia coli in Korean veterinary hospitals</title><author>So, Jeong Hwa ; Kim, Juwon ; Bae, Il Kwon ; Jeong, Seok Hoon ; Kim, So Hyun ; Lim, Suk-kyung ; Park, Yong Ho ; Lee, Kyungwon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c564t-3392b426efb2875a42e4be8cf997b8b0eb32e0a0dd9076a3d79cd7c25579058b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacteriology</topic><topic>beta-Lactamases - genetics</topic><topic>Biological and medical sciences</topic><topic>Carrier State - microbiology</topic><topic>Carrier State - veterinary</topic><topic>CMY-2</topic><topic>Companion animal</topic><topic>CTX-M</topic><topic>DNA, Bacterial - analysis</topic><topic>Dogs</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hospitals, Animal - statistics & numerical data</topic><topic>IncF plasmid</topic><topic>Infectious Disease</topic><topic>Infectious diseases</topic><topic>Internal Medicine</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Rectum - microbiology</topic><topic>Republic of Korea</topic><topic>rmtB</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>So, Jeong Hwa</creatorcontrib><creatorcontrib>Kim, Juwon</creatorcontrib><creatorcontrib>Bae, Il Kwon</creatorcontrib><creatorcontrib>Jeong, Seok Hoon</creatorcontrib><creatorcontrib>Kim, So Hyun</creatorcontrib><creatorcontrib>Lim, Suk-kyung</creatorcontrib><creatorcontrib>Park, Yong Ho</creatorcontrib><creatorcontrib>Lee, Kyungwon</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>So, Jeong Hwa</au><au>Kim, Juwon</au><au>Bae, Il Kwon</au><au>Jeong, Seok Hoon</au><au>Kim, So Hyun</au><au>Lim, Suk-kyung</au><au>Park, Yong Ho</au><au>Lee, Kyungwon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dissemination of multidrug-resistant Escherichia coli in Korean veterinary hospitals</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>73</volume><issue>2</issue><spage>195</spage><epage>199</epage><pages>195-199</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>Abstract This study was performed to investigate the prevalence of rectal colonization with multidrug-resistant Escherichia coli in dogs hospitalized at veterinary hospitals in Korea and to assess the molecular epidemiologic traits of this organism. A total of 63 unique E. coli isolates obtained from the rectal swabs of hospitalized dogs were analyzed. Genes encoding CTX-M extended-spectrum β-lactamases (ESBLs) and AmpC enzymes were detected in 21 (33.3%) and 15 (23.8%) canine E. coli isolates, respectively. Twelve canine E. coli isolates harbored both the genes encoding the CTX-M and AmpC enzymes. Six ESBL-producing E. coli isolates also carried the rmtB gene. All 24 E. coli isolates producing CTX-M ESBL and/or CMY-2 were resistant to ciprofloxacin. Furthermore, mutations were found in the gyrA and the parC genes. In most cases, the bla genes of the CTX-M ESBL and AmpC enzymes and the rmtB gene were localized to incompatibility group F (IncF) plasmids. Possible small clonal outbreaks are suggested because some E. coli isolates recovered in the same veterinary hospital were identified as identical sequence types and showed identical banding patterns in repetitive sequence-based polymerase chain reaction. The horizontal transfer of IncF plasmids and the clonal transfer of E. coli strains are suggested to play a role in the dissemination of antimicrobial resistance genes, and this transfer may occur across host species (i.e., between humans and dogs).</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>22516765</pmid><doi>10.1016/j.diagmicrobio.2012.03.010</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Anti-Bacterial Agents - pharmacology Bacteriology beta-Lactamases - genetics Biological and medical sciences Carrier State - microbiology Carrier State - veterinary CMY-2 Companion animal CTX-M DNA, Bacterial - analysis Dogs Drug Resistance, Multiple, Bacterial Escherichia coli Escherichia coli - drug effects Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Escherichia coli Proteins - genetics Fundamental and applied biological sciences. Psychology Hospitals, Animal - statistics & numerical data IncF plasmid Infectious Disease Infectious diseases Internal Medicine Medical sciences Microbial Sensitivity Tests Microbiology Miscellaneous Plasmids Polymerase Chain Reaction Rectum - microbiology Republic of Korea rmtB |
| title | Dissemination of multidrug-resistant Escherichia coli in Korean veterinary hospitals |
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