Quantification of ZP1, ZP2 and ZP3 mRNA of marmoset monkey (Callithrix jacchus) oocytes from periantral and antral follicles
Summary In mammals, the oocyte and preimplantation embryo are protected by the zona pellucida (ZP) consisting mainly of ZP glycoproteins, which are responsible for sperm binding, induction of the acrosome reaction and zona pellucida hardening to prevent polyspermia. The ZP proteins become increasing...
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| Veröffentlicht in: | Andrologia 2012-05, Vol.44 (s1), p.349-353 |
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| creator | Konrad, L. Kuhnert, S. Nayudu, P. L. Einspanier, R. Hinsch, K. D. Hinsch, E. |
| description | Summary
In mammals, the oocyte and preimplantation embryo are protected by the zona pellucida (ZP) consisting mainly of ZP glycoproteins, which are responsible for sperm binding, induction of the acrosome reaction and zona pellucida hardening to prevent polyspermia. The ZP proteins become increasingly important as possible predictors for in vitro cultured oocytes competence. As little is known about the stage‐dependent expression of ZP1, ZP2 and ZP3 in marmoset monkey (Callithrix jacchus) oocytes, mRNA expression was investigated with real‐time RT‐PCR. Total‐RNA was isolated from three different classes of marmoset oocytes; Class 1 oocytes from periantral follicles (1000 μm, n = 9). Compared with Class 1 oocytes mRNA expression of ZP1, ZP2 and ZP3 in Class 2 oocytes was significantly decreased. In Class 3 oocytes, the transcription of ZP1, ZP2 and ZP3 genes showed also a significant decrease compared with Class 1 oocytes. In this study a differently regulated expression of the ZP genes during late folliculogenesis with an obvious downregulation of ZP1, ZP2 and ZP3 could be demonstrated for the first time in the marmoset monkey. |
| doi_str_mv | 10.1111/j.1439-0272.2011.01188.x |
| format | Article |
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In mammals, the oocyte and preimplantation embryo are protected by the zona pellucida (ZP) consisting mainly of ZP glycoproteins, which are responsible for sperm binding, induction of the acrosome reaction and zona pellucida hardening to prevent polyspermia. The ZP proteins become increasingly important as possible predictors for in vitro cultured oocytes competence. As little is known about the stage‐dependent expression of ZP1, ZP2 and ZP3 in marmoset monkey (Callithrix jacchus) oocytes, mRNA expression was investigated with real‐time RT‐PCR. Total‐RNA was isolated from three different classes of marmoset oocytes; Class 1 oocytes from periantral follicles (<600 μm, n = 10), Class 2 oocytes from small antral follicles (600–1000 μm, n = 10) and Class 3 oocytes from large antral follicles (>1000 μm, n = 9). Compared with Class 1 oocytes mRNA expression of ZP1, ZP2 and ZP3 in Class 2 oocytes was significantly decreased. In Class 3 oocytes, the transcription of ZP1, ZP2 and ZP3 genes showed also a significant decrease compared with Class 1 oocytes. In this study a differently regulated expression of the ZP genes during late folliculogenesis with an obvious downregulation of ZP1, ZP2 and ZP3 could be demonstrated for the first time in the marmoset monkey.</description><identifier>ISSN: 0303-4569</identifier><identifier>EISSN: 1439-0272</identifier><identifier>DOI: 10.1111/j.1439-0272.2011.01188.x</identifier><identifier>PMID: 21689136</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acrosome reaction ; Animals ; Base Sequence ; Callithrix ; Callithrix jacchus ; DNA Primers ; Egg Proteins - genetics ; Embryos ; Female ; Follicles ; Gene expression ; Glycoproteins ; Marmoset ; Membrane Glycoproteins - genetics ; Oocytes ; Oocytes - metabolism ; Ovarian Follicle - metabolism ; Polymerase chain reaction ; quantification ; real-time PCR ; Real-Time Polymerase Chain Reaction ; Receptors, Cell Surface - genetics ; RNA, Messenger - genetics ; Sperm ; Transcription ; Zona pellucida ; Zona Pellucida Glycoproteins</subject><ispartof>Andrologia, 2012-05, Vol.44 (s1), p.349-353</ispartof><rights>2011 Blackwell Verlag GmbH</rights><rights>2011 Blackwell Verlag GmbH.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3918-577a543331e40bb591d7d8d52aa43defc5774c52154c7b6440502f25dbe93de13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0272.2011.01188.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0272.2011.01188.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21689136$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Konrad, L.</creatorcontrib><creatorcontrib>Kuhnert, S.</creatorcontrib><creatorcontrib>Nayudu, P. L.</creatorcontrib><creatorcontrib>Einspanier, R.</creatorcontrib><creatorcontrib>Hinsch, K. D.</creatorcontrib><creatorcontrib>Hinsch, E.</creatorcontrib><title>Quantification of ZP1, ZP2 and ZP3 mRNA of marmoset monkey (Callithrix jacchus) oocytes from periantral and antral follicles</title><title>Andrologia</title><addtitle>Andrologia</addtitle><description>Summary
In mammals, the oocyte and preimplantation embryo are protected by the zona pellucida (ZP) consisting mainly of ZP glycoproteins, which are responsible for sperm binding, induction of the acrosome reaction and zona pellucida hardening to prevent polyspermia. The ZP proteins become increasingly important as possible predictors for in vitro cultured oocytes competence. As little is known about the stage‐dependent expression of ZP1, ZP2 and ZP3 in marmoset monkey (Callithrix jacchus) oocytes, mRNA expression was investigated with real‐time RT‐PCR. Total‐RNA was isolated from three different classes of marmoset oocytes; Class 1 oocytes from periantral follicles (<600 μm, n = 10), Class 2 oocytes from small antral follicles (600–1000 μm, n = 10) and Class 3 oocytes from large antral follicles (>1000 μm, n = 9). Compared with Class 1 oocytes mRNA expression of ZP1, ZP2 and ZP3 in Class 2 oocytes was significantly decreased. In Class 3 oocytes, the transcription of ZP1, ZP2 and ZP3 genes showed also a significant decrease compared with Class 1 oocytes. In this study a differently regulated expression of the ZP genes during late folliculogenesis with an obvious downregulation of ZP1, ZP2 and ZP3 could be demonstrated for the first time in the marmoset monkey.</description><subject>Acrosome reaction</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Callithrix</subject><subject>Callithrix jacchus</subject><subject>DNA Primers</subject><subject>Egg Proteins - genetics</subject><subject>Embryos</subject><subject>Female</subject><subject>Follicles</subject><subject>Gene expression</subject><subject>Glycoproteins</subject><subject>Marmoset</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Oocytes</subject><subject>Oocytes - metabolism</subject><subject>Ovarian Follicle - metabolism</subject><subject>Polymerase chain reaction</subject><subject>quantification</subject><subject>real-time PCR</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptors, Cell Surface - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Sperm</subject><subject>Transcription</subject><subject>Zona pellucida</subject><subject>Zona Pellucida Glycoproteins</subject><issn>0303-4569</issn><issn>1439-0272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUVtr2zAUFmNlDWn_QtFjBrOnqy0_7CFkWzII6TraFPYiZFkmSm0rtWyWQH985SbLcw9I58B3QTofABCjGIf6uo0xo1mESEpigjCOwxEi3n8AozPwEYwQRTRiPMkuwbX3WxSK8TRl7BO4JDgRGabJCLzc9arpbGm16qxroCvh39_4S7gIVE0ROoX1n9V0AGrV1s6bDtaueTIHOJmpqrLdprV7uFVab3r_GTqnD53xsGxdDXemtcG-VdWb2WksXZDpyvgrcFGqypvrUx-Dh58_7meLaHk7_zWbLiNNMyyi8GrFGaUUG4bynGe4SAtRcKIUo4Up9fAtzQnmTKd5whjiiJSEF7nJAo7pGEyOvrvWPffGd7K2XpuqUo1xvZcYEZbwBAn-DioWNBEoFYF6c6L2eW0KuWtt2NBB_l9uIHw7Ev7ZyhzOOEZyyFFu5RCXHOKSQ47yLUe5l9PV92EK-uiot74z-7NetU8ySWnK5eNqLpeL1Wx-t17LNX0FhyKeNg</recordid><startdate>201205</startdate><enddate>201205</enddate><creator>Konrad, L.</creator><creator>Kuhnert, S.</creator><creator>Nayudu, P. L.</creator><creator>Einspanier, R.</creator><creator>Hinsch, K. D.</creator><creator>Hinsch, E.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>201205</creationdate><title>Quantification of ZP1, ZP2 and ZP3 mRNA of marmoset monkey (Callithrix jacchus) oocytes from periantral and antral follicles</title><author>Konrad, L. ; Kuhnert, S. ; Nayudu, P. L. ; Einspanier, R. ; Hinsch, K. D. ; Hinsch, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3918-577a543331e40bb591d7d8d52aa43defc5774c52154c7b6440502f25dbe93de13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acrosome reaction</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Callithrix</topic><topic>Callithrix jacchus</topic><topic>DNA Primers</topic><topic>Egg Proteins - genetics</topic><topic>Embryos</topic><topic>Female</topic><topic>Follicles</topic><topic>Gene expression</topic><topic>Glycoproteins</topic><topic>Marmoset</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Oocytes</topic><topic>Oocytes - metabolism</topic><topic>Ovarian Follicle - metabolism</topic><topic>Polymerase chain reaction</topic><topic>quantification</topic><topic>real-time PCR</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptors, Cell Surface - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Sperm</topic><topic>Transcription</topic><topic>Zona pellucida</topic><topic>Zona Pellucida Glycoproteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Konrad, L.</creatorcontrib><creatorcontrib>Kuhnert, S.</creatorcontrib><creatorcontrib>Nayudu, P. L.</creatorcontrib><creatorcontrib>Einspanier, R.</creatorcontrib><creatorcontrib>Hinsch, K. D.</creatorcontrib><creatorcontrib>Hinsch, E.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Andrologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Konrad, L.</au><au>Kuhnert, S.</au><au>Nayudu, P. L.</au><au>Einspanier, R.</au><au>Hinsch, K. D.</au><au>Hinsch, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of ZP1, ZP2 and ZP3 mRNA of marmoset monkey (Callithrix jacchus) oocytes from periantral and antral follicles</atitle><jtitle>Andrologia</jtitle><addtitle>Andrologia</addtitle><date>2012-05</date><risdate>2012</risdate><volume>44</volume><issue>s1</issue><spage>349</spage><epage>353</epage><pages>349-353</pages><issn>0303-4569</issn><eissn>1439-0272</eissn><abstract>Summary
In mammals, the oocyte and preimplantation embryo are protected by the zona pellucida (ZP) consisting mainly of ZP glycoproteins, which are responsible for sperm binding, induction of the acrosome reaction and zona pellucida hardening to prevent polyspermia. The ZP proteins become increasingly important as possible predictors for in vitro cultured oocytes competence. As little is known about the stage‐dependent expression of ZP1, ZP2 and ZP3 in marmoset monkey (Callithrix jacchus) oocytes, mRNA expression was investigated with real‐time RT‐PCR. Total‐RNA was isolated from three different classes of marmoset oocytes; Class 1 oocytes from periantral follicles (<600 μm, n = 10), Class 2 oocytes from small antral follicles (600–1000 μm, n = 10) and Class 3 oocytes from large antral follicles (>1000 μm, n = 9). Compared with Class 1 oocytes mRNA expression of ZP1, ZP2 and ZP3 in Class 2 oocytes was significantly decreased. In Class 3 oocytes, the transcription of ZP1, ZP2 and ZP3 genes showed also a significant decrease compared with Class 1 oocytes. In this study a differently regulated expression of the ZP genes during late folliculogenesis with an obvious downregulation of ZP1, ZP2 and ZP3 could be demonstrated for the first time in the marmoset monkey.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21689136</pmid><doi>10.1111/j.1439-0272.2011.01188.x</doi><tpages>5</tpages></addata></record> |
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| subjects | Acrosome reaction Animals Base Sequence Callithrix Callithrix jacchus DNA Primers Egg Proteins - genetics Embryos Female Follicles Gene expression Glycoproteins Marmoset Membrane Glycoproteins - genetics Oocytes Oocytes - metabolism Ovarian Follicle - metabolism Polymerase chain reaction quantification real-time PCR Real-Time Polymerase Chain Reaction Receptors, Cell Surface - genetics RNA, Messenger - genetics Sperm Transcription Zona pellucida Zona Pellucida Glycoproteins |
| title | Quantification of ZP1, ZP2 and ZP3 mRNA of marmoset monkey (Callithrix jacchus) oocytes from periantral and antral follicles |
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