Decreased body weight in young Osterix-Cre transgenic mice results in delayed cortical bone expansion and accrual
Conditional gene inactivation using the Cre/loxP system has lead to significant advances in our understanding of the function of genes in a wide range of disciplines. It is becoming increasingly apparent in the literature, that Cre transgenic mice may themselves have a phenotype. In the following st...
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| Veröffentlicht in: | Transgenic research 2012-08, Vol.21 (4), p.885-893 |
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| creator | Davey, Rachel A Clarke, Michele V Sastra, Stephen Skinner, Jarrod P Chiang, Cherie Anderson, Paul H Zajac, Jeffrey D |
| description | Conditional gene inactivation using the Cre/loxP system has lead to significant advances in our understanding of the function of genes in a wide range of disciplines. It is becoming increasingly apparent in the literature, that Cre transgenic mice may themselves have a phenotype. In the following study we describe the bone phenotype of a commonly used Cre transgenic mouse line to study osteoblasts, the Osx-GFP::Cre (Osx-Cre) mice. Cortical and trabecular bone parameters were determined in the femurs of Osx-Cre mice at 6 and 12 weeks of age by microtomography (μCT). At 6 weeks of age, Osx-Cre mice had reduced body weight by 22% (P |
| doi_str_mv | 10.1007/s11248-011-9581-z |
| format | Article |
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It is becoming increasingly apparent in the literature, that Cre transgenic mice may themselves have a phenotype. In the following study we describe the bone phenotype of a commonly used Cre transgenic mouse line to study osteoblasts, the Osx-GFP::Cre (Osx-Cre) mice. Cortical and trabecular bone parameters were determined in the femurs of Osx-Cre mice at 6 and 12 weeks of age by microtomography (μCT). At 6 weeks of age, Osx-Cre mice had reduced body weight by 22% (P < 0.0001) and delayed cortical bone expansion and accrual, characterized by decreases in periosteal circumference by 7% (P < 0.05) and cortical thickness by 11% (P < 0.01), compared to wild type controls. Importantly, the cortical bone phenotype of the skeletally immature Osx-Cre mice at 6 weeks of age could be accounted for by their low body weight. The delayed weight gain and cortical growth of Osx-Cre mice was overcome by 12 weeks of age, with no differences observed between Osx-Cre and wild type controls. In conclusion, Osx-Cre expressing mice display a delayed growth phenotype in the absence of doxycycline treatment, evidenced by decreased cortical bone expansion and accrual at 6 weeks of age, as an indirect result of decreased body weight. While this delay in growth is overcome by adulthood at 12 weeks of age, caution together with appropriate data analysis must be considered when assessing the experimental data from skeletally immature Cre/loxP knockout mice generated using the Osx-Cre mouse line to avoid misinterpretation.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1007/s11248-011-9581-z</identifier><identifier>PMID: 22160436</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>adulthood ; Animal Genetics and Genomics ; Animals ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biotechnology ; Body Weight - genetics ; Body Weight - physiology ; Bone and Bones - diagnostic imaging ; Bone and Bones - physiology ; Bone Development - genetics ; Bone Development - physiology ; Brief Communication ; doxycycline ; Femur - diagnostic imaging ; Fundamental and applied biological sciences. Psychology ; genes ; Genetic Engineering ; Genetic technics ; Integrases - genetics ; Integrases - metabolism ; Life Sciences ; Methods. Procedures. Technologies ; Mice ; Mice, Transgenic - genetics ; Mice, Transgenic - physiology ; Molecular Medicine ; osteoblasts ; Phenotype ; Plant Genetics and Genomics ; Radiography ; Sp7 Transcription Factor ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transgenic animals and transgenic plants ; Transgenics ; weight gain</subject><ispartof>Transgenic research, 2012-08, Vol.21 (4), p.885-893</ispartof><rights>Springer Science+Business Media B.V. 2011</rights><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-1e729c508d29cffcd65afeadf68fe34ad5f6d6b50c9cebf79eaa5762c83da0a63</citedby><cites>FETCH-LOGICAL-c535t-1e729c508d29cffcd65afeadf68fe34ad5f6d6b50c9cebf79eaa5762c83da0a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11248-011-9581-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11248-011-9581-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26175505$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22160436$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Davey, Rachel A</creatorcontrib><creatorcontrib>Clarke, Michele V</creatorcontrib><creatorcontrib>Sastra, Stephen</creatorcontrib><creatorcontrib>Skinner, Jarrod P</creatorcontrib><creatorcontrib>Chiang, Cherie</creatorcontrib><creatorcontrib>Anderson, Paul H</creatorcontrib><creatorcontrib>Zajac, Jeffrey D</creatorcontrib><title>Decreased body weight in young Osterix-Cre transgenic mice results in delayed cortical bone expansion and accrual</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><addtitle>Transgenic Res</addtitle><description>Conditional gene inactivation using the Cre/loxP system has lead to significant advances in our understanding of the function of genes in a wide range of disciplines. It is becoming increasingly apparent in the literature, that Cre transgenic mice may themselves have a phenotype. In the following study we describe the bone phenotype of a commonly used Cre transgenic mouse line to study osteoblasts, the Osx-GFP::Cre (Osx-Cre) mice. Cortical and trabecular bone parameters were determined in the femurs of Osx-Cre mice at 6 and 12 weeks of age by microtomography (μCT). At 6 weeks of age, Osx-Cre mice had reduced body weight by 22% (P < 0.0001) and delayed cortical bone expansion and accrual, characterized by decreases in periosteal circumference by 7% (P < 0.05) and cortical thickness by 11% (P < 0.01), compared to wild type controls. Importantly, the cortical bone phenotype of the skeletally immature Osx-Cre mice at 6 weeks of age could be accounted for by their low body weight. The delayed weight gain and cortical growth of Osx-Cre mice was overcome by 12 weeks of age, with no differences observed between Osx-Cre and wild type controls. In conclusion, Osx-Cre expressing mice display a delayed growth phenotype in the absence of doxycycline treatment, evidenced by decreased cortical bone expansion and accrual at 6 weeks of age, as an indirect result of decreased body weight. While this delay in growth is overcome by adulthood at 12 weeks of age, caution together with appropriate data analysis must be considered when assessing the experimental data from skeletally immature Cre/loxP knockout mice generated using the Osx-Cre mouse line to avoid misinterpretation.</description><subject>adulthood</subject><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biotechnology</subject><subject>Body Weight - genetics</subject><subject>Body Weight - physiology</subject><subject>Bone and Bones - diagnostic imaging</subject><subject>Bone and Bones - physiology</subject><subject>Bone Development - genetics</subject><subject>Bone Development - physiology</subject><subject>Brief Communication</subject><subject>doxycycline</subject><subject>Femur - diagnostic imaging</subject><subject>Fundamental and applied biological sciences. 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Technologies</subject><subject>Mice</subject><subject>Mice, Transgenic - genetics</subject><subject>Mice, Transgenic - physiology</subject><subject>Molecular Medicine</subject><subject>osteoblasts</subject><subject>Phenotype</subject><subject>Plant Genetics and Genomics</subject><subject>Radiography</subject><subject>Sp7 Transcription Factor</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenics</subject><subject>weight gain</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kUtv1DAUhS0EokPhB7ABSwiJTYofsZMs0fCUKnVRuo7u2NfBVcae2ono9NfjaYaHkLq6i_udc4_uIeQlZ2ecseZ95lzUbcU4rzrV8uruEVlx1ciqk7p9TFas06JqW96dkGc5XzNWVK18Sk6E4JrVUq_IzUc0CSGjpZto9_Qn-uHHRH2g-ziHgV7kCZO_rdYJ6ZQg5AGDN3TrDdKEeR6nfIAtjrAvHiamyRsYi1lAire7ovAxUAiWgjFphvE5eeJgzPjiOE_J1edP39dfq_OLL9_WH84ro6SaKo6N6IxirS3DOWO1AodgnW4dyhqsctrqjWKmM7hxTYcAqtHCtNICAy1PybvFd5fizYx56rc-GxxHCBjn3HMmal0r1qmCvvkPvY5zCiXdPVULqbq6UHyhTIo5J3T9LvktpH2B-kMf_dJHX_roD330d0Xz6ug8b7Zo_yh-F1CAt0cAcvmbKy82Pv_lNG-UYoeIYuFyWYUB078RH77-ehE5iD0MqRhfXQrGa8aYbpRQ8hePy64z</recordid><startdate>20120801</startdate><enddate>20120801</enddate><creator>Davey, Rachel A</creator><creator>Clarke, Michele V</creator><creator>Sastra, Stephen</creator><creator>Skinner, Jarrod P</creator><creator>Chiang, Cherie</creator><creator>Anderson, Paul H</creator><creator>Zajac, Jeffrey D</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20120801</creationdate><title>Decreased body weight in young Osterix-Cre transgenic mice results in delayed cortical bone expansion and accrual</title><author>Davey, Rachel A ; Clarke, Michele V ; Sastra, Stephen ; Skinner, Jarrod P ; Chiang, Cherie ; Anderson, Paul H ; Zajac, Jeffrey D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c535t-1e729c508d29cffcd65afeadf68fe34ad5f6d6b50c9cebf79eaa5762c83da0a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>adulthood</topic><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biotechnology</topic><topic>Body Weight - genetics</topic><topic>Body Weight - physiology</topic><topic>Bone and Bones - diagnostic imaging</topic><topic>Bone and Bones - physiology</topic><topic>Bone Development - genetics</topic><topic>Bone Development - physiology</topic><topic>Brief Communication</topic><topic>doxycycline</topic><topic>Femur - diagnostic imaging</topic><topic>Fundamental and applied biological sciences. 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Technologies</topic><topic>Mice</topic><topic>Mice, Transgenic - genetics</topic><topic>Mice, Transgenic - physiology</topic><topic>Molecular Medicine</topic><topic>osteoblasts</topic><topic>Phenotype</topic><topic>Plant Genetics and Genomics</topic><topic>Radiography</topic><topic>Sp7 Transcription Factor</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenics</topic><topic>weight gain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Davey, Rachel A</creatorcontrib><creatorcontrib>Clarke, Michele V</creatorcontrib><creatorcontrib>Sastra, Stephen</creatorcontrib><creatorcontrib>Skinner, Jarrod P</creatorcontrib><creatorcontrib>Chiang, Cherie</creatorcontrib><creatorcontrib>Anderson, Paul H</creatorcontrib><creatorcontrib>Zajac, Jeffrey D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Davey, Rachel A</au><au>Clarke, Michele V</au><au>Sastra, Stephen</au><au>Skinner, Jarrod P</au><au>Chiang, Cherie</au><au>Anderson, Paul H</au><au>Zajac, Jeffrey D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased body weight in young Osterix-Cre transgenic mice results in delayed cortical bone expansion and accrual</atitle><jtitle>Transgenic research</jtitle><stitle>Transgenic Res</stitle><addtitle>Transgenic Res</addtitle><date>2012-08-01</date><risdate>2012</risdate><volume>21</volume><issue>4</issue><spage>885</spage><epage>893</epage><pages>885-893</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>Conditional gene inactivation using the Cre/loxP system has lead to significant advances in our understanding of the function of genes in a wide range of disciplines. It is becoming increasingly apparent in the literature, that Cre transgenic mice may themselves have a phenotype. In the following study we describe the bone phenotype of a commonly used Cre transgenic mouse line to study osteoblasts, the Osx-GFP::Cre (Osx-Cre) mice. Cortical and trabecular bone parameters were determined in the femurs of Osx-Cre mice at 6 and 12 weeks of age by microtomography (μCT). At 6 weeks of age, Osx-Cre mice had reduced body weight by 22% (P < 0.0001) and delayed cortical bone expansion and accrual, characterized by decreases in periosteal circumference by 7% (P < 0.05) and cortical thickness by 11% (P < 0.01), compared to wild type controls. Importantly, the cortical bone phenotype of the skeletally immature Osx-Cre mice at 6 weeks of age could be accounted for by their low body weight. The delayed weight gain and cortical growth of Osx-Cre mice was overcome by 12 weeks of age, with no differences observed between Osx-Cre and wild type controls. In conclusion, Osx-Cre expressing mice display a delayed growth phenotype in the absence of doxycycline treatment, evidenced by decreased cortical bone expansion and accrual at 6 weeks of age, as an indirect result of decreased body weight. While this delay in growth is overcome by adulthood at 12 weeks of age, caution together with appropriate data analysis must be considered when assessing the experimental data from skeletally immature Cre/loxP knockout mice generated using the Osx-Cre mouse line to avoid misinterpretation.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><pmid>22160436</pmid><doi>10.1007/s11248-011-9581-z</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | adulthood Animal Genetics and Genomics Animals Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biotechnology Body Weight - genetics Body Weight - physiology Bone and Bones - diagnostic imaging Bone and Bones - physiology Bone Development - genetics Bone Development - physiology Brief Communication doxycycline Femur - diagnostic imaging Fundamental and applied biological sciences. Psychology genes Genetic Engineering Genetic technics Integrases - genetics Integrases - metabolism Life Sciences Methods. Procedures. Technologies Mice Mice, Transgenic - genetics Mice, Transgenic - physiology Molecular Medicine osteoblasts Phenotype Plant Genetics and Genomics Radiography Sp7 Transcription Factor Transcription Factors - genetics Transcription Factors - metabolism Transgenic animals and transgenic plants Transgenics weight gain |
| title | Decreased body weight in young Osterix-Cre transgenic mice results in delayed cortical bone expansion and accrual |
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