Biological behavior of different Trypanosoma cruzi isolates circulating in an endemic area for Chagas disease in the Gran Chaco region of Argentina
This work describes the biological characterization of Trypanosoma cruzi isolates sympatrically circulating in an endemic region of Chagas disease in Chaco region, in Argentina. [Display omitted] ► The biological behavior of Trypanosoma cruzi strains is still obscure. ► Four T. cruzi DTUs were found...
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| Veröffentlicht in: | Acta tropica 2012-09, Vol.123 (3), p.196-201 |
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| creator | Ragone, Paula G. Pérez Brandán, Cecilia Padilla, Angel M. Monje Rumi, Mercedes Lauthier, Juan J. Alberti D’Amato, Anahí M. Tomasini, Nicolás Cimino, Ruben O. Romero, Nélida M. Portelli, Marcela Nasser, Julio R. Basombrío, Miguel A. Diosque, Patricio |
| description | This work describes the biological characterization of Trypanosoma cruzi isolates sympatrically circulating in an endemic region of Chagas disease in Chaco region, in Argentina. [Display omitted]
► The biological behavior of Trypanosoma cruzi strains is still obscure. ► Four T. cruzi DTUs were found in the study area in Argentina. ► A vector transmission model was developed to maintain T. cruzi isolates. ► Different parasite load, serological response and tissue damage among isolates was detected. ► Results let us formulate hypotheses of clinical and epidemiological importance.
The biological behavior of the different Trypanosoma cruzi strains is still unclear and the importance of exploring the relevance of these differences in natural isolates is of great significance. Herein we describe the biological behavior of four T. cruzi isolates circulating sympatrically in a restricted geographic area in Argentina endemic for Chagas Disease. These isolates were characterized as belonging to the Discrete Typing Units (DTUs) TcI, TcIII, TcV and TcVI as shown by Multilocus Enzyme Electrophoresis and Multilocus Sequence Typing. In order to study the natural behavior of the different isolates and to preserve their natural properties, we developed a vector transmission model that allows their maintenance in the laboratory. The model consisted of serial passages of these parasites between insect vectors and mice. Vector-derived parasite forms were then inoculated in C57BL/6J mice and number of parasite in peripheral blood, serological response and histological damage in acute and chronic phases of the infection were measured. Parasites from DTUs TcI, TcIII and TcVI were detected by direct fresh blood examination, while TcV parasites could only be detected by Polimerase Chain Reaction. No significant difference in the anti-T. cruzi antibody response was found during the chronic phase of infection, except for mice infected with TcV parasites where no antibodies could be detected. Histological sections showed that TcI isolate produced more damage in skeletal muscle while TcVI induced more inflammation in the heart. This work shows differential biological behavior among different parasite isolates obtained from the same cycle of transmission, permitting the opportunity to formulate future hypotheses of clinical and epidemiological importance. |
| doi_str_mv | 10.1016/j.actatropica.2012.05.003 |
| format | Article |
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► The biological behavior of Trypanosoma cruzi strains is still obscure. ► Four T. cruzi DTUs were found in the study area in Argentina. ► A vector transmission model was developed to maintain T. cruzi isolates. ► Different parasite load, serological response and tissue damage among isolates was detected. ► Results let us formulate hypotheses of clinical and epidemiological importance.
The biological behavior of the different Trypanosoma cruzi strains is still unclear and the importance of exploring the relevance of these differences in natural isolates is of great significance. Herein we describe the biological behavior of four T. cruzi isolates circulating sympatrically in a restricted geographic area in Argentina endemic for Chagas Disease. These isolates were characterized as belonging to the Discrete Typing Units (DTUs) TcI, TcIII, TcV and TcVI as shown by Multilocus Enzyme Electrophoresis and Multilocus Sequence Typing. In order to study the natural behavior of the different isolates and to preserve their natural properties, we developed a vector transmission model that allows their maintenance in the laboratory. The model consisted of serial passages of these parasites between insect vectors and mice. Vector-derived parasite forms were then inoculated in C57BL/6J mice and number of parasite in peripheral blood, serological response and histological damage in acute and chronic phases of the infection were measured. Parasites from DTUs TcI, TcIII and TcVI were detected by direct fresh blood examination, while TcV parasites could only be detected by Polimerase Chain Reaction. No significant difference in the anti-T. cruzi antibody response was found during the chronic phase of infection, except for mice infected with TcV parasites where no antibodies could be detected. Histological sections showed that TcI isolate produced more damage in skeletal muscle while TcVI induced more inflammation in the heart. This work shows differential biological behavior among different parasite isolates obtained from the same cycle of transmission, permitting the opportunity to formulate future hypotheses of clinical and epidemiological importance.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/j.actatropica.2012.05.003</identifier><identifier>PMID: 22643298</identifier><identifier>CODEN: ACTRAQ</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Animals ; antibodies ; Antibodies, Protozoan - blood ; Argentina - epidemiology ; Biological and medical sciences ; Biological properties ; blood ; Blood - parasitology ; Chagas disease ; Chagas Disease - epidemiology ; Chagas Disease - immunology ; Chagas Disease - parasitology ; Chagas Disease - pathology ; Disease Models, Animal ; DNA Fingerprinting ; DNA, Protozoan - genetics ; electrophoresis ; Endemic Diseases ; Enzymes - analysis ; General aspects ; Genetic Variation ; heart ; Heart - parasitology ; Human protozoal diseases ; Humans ; Infectious diseases ; inflammation ; insect vectors ; Male ; Medical sciences ; Mice ; Mice, Inbred C57BL ; multilocus sequence typing ; Muscle, Skeletal - parasitology ; Muscle, Skeletal - pathology ; Myocardium - pathology ; Natural isolates ; parasites ; Parasitic diseases ; Protozoal diseases ; skeletal muscle ; Trypanosoma cruzi ; Trypanosoma cruzi - classification ; Trypanosoma cruzi - isolation & purification ; Trypanosoma cruzi - pathogenicity ; Trypanosomiasis</subject><ispartof>Acta tropica, 2012-09, Vol.123 (3), p.196-201</ispartof><rights>2012 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-18787ad8046fdf4de52221635f1555d183bf09a55296ad770ddab7bbc3f207363</citedby><cites>FETCH-LOGICAL-c482t-18787ad8046fdf4de52221635f1555d183bf09a55296ad770ddab7bbc3f207363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.actatropica.2012.05.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26132956$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22643298$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ragone, Paula G.</creatorcontrib><creatorcontrib>Pérez Brandán, Cecilia</creatorcontrib><creatorcontrib>Padilla, Angel M.</creatorcontrib><creatorcontrib>Monje Rumi, Mercedes</creatorcontrib><creatorcontrib>Lauthier, Juan J.</creatorcontrib><creatorcontrib>Alberti D’Amato, Anahí M.</creatorcontrib><creatorcontrib>Tomasini, Nicolás</creatorcontrib><creatorcontrib>Cimino, Ruben O.</creatorcontrib><creatorcontrib>Romero, Nélida M.</creatorcontrib><creatorcontrib>Portelli, Marcela</creatorcontrib><creatorcontrib>Nasser, Julio R.</creatorcontrib><creatorcontrib>Basombrío, Miguel A.</creatorcontrib><creatorcontrib>Diosque, Patricio</creatorcontrib><title>Biological behavior of different Trypanosoma cruzi isolates circulating in an endemic area for Chagas disease in the Gran Chaco region of Argentina</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>This work describes the biological characterization of Trypanosoma cruzi isolates sympatrically circulating in an endemic region of Chagas disease in Chaco region, in Argentina. [Display omitted]
► The biological behavior of Trypanosoma cruzi strains is still obscure. ► Four T. cruzi DTUs were found in the study area in Argentina. ► A vector transmission model was developed to maintain T. cruzi isolates. ► Different parasite load, serological response and tissue damage among isolates was detected. ► Results let us formulate hypotheses of clinical and epidemiological importance.
The biological behavior of the different Trypanosoma cruzi strains is still unclear and the importance of exploring the relevance of these differences in natural isolates is of great significance. Herein we describe the biological behavior of four T. cruzi isolates circulating sympatrically in a restricted geographic area in Argentina endemic for Chagas Disease. These isolates were characterized as belonging to the Discrete Typing Units (DTUs) TcI, TcIII, TcV and TcVI as shown by Multilocus Enzyme Electrophoresis and Multilocus Sequence Typing. In order to study the natural behavior of the different isolates and to preserve their natural properties, we developed a vector transmission model that allows their maintenance in the laboratory. The model consisted of serial passages of these parasites between insect vectors and mice. Vector-derived parasite forms were then inoculated in C57BL/6J mice and number of parasite in peripheral blood, serological response and histological damage in acute and chronic phases of the infection were measured. Parasites from DTUs TcI, TcIII and TcVI were detected by direct fresh blood examination, while TcV parasites could only be detected by Polimerase Chain Reaction. No significant difference in the anti-T. cruzi antibody response was found during the chronic phase of infection, except for mice infected with TcV parasites where no antibodies could be detected. Histological sections showed that TcI isolate produced more damage in skeletal muscle while TcVI induced more inflammation in the heart. This work shows differential biological behavior among different parasite isolates obtained from the same cycle of transmission, permitting the opportunity to formulate future hypotheses of clinical and epidemiological importance.</description><subject>Animals</subject><subject>antibodies</subject><subject>Antibodies, Protozoan - blood</subject><subject>Argentina - epidemiology</subject><subject>Biological and medical sciences</subject><subject>Biological properties</subject><subject>blood</subject><subject>Blood - parasitology</subject><subject>Chagas disease</subject><subject>Chagas Disease - epidemiology</subject><subject>Chagas Disease - immunology</subject><subject>Chagas Disease - parasitology</subject><subject>Chagas Disease - pathology</subject><subject>Disease Models, Animal</subject><subject>DNA Fingerprinting</subject><subject>DNA, Protozoan - genetics</subject><subject>electrophoresis</subject><subject>Endemic Diseases</subject><subject>Enzymes - analysis</subject><subject>General aspects</subject><subject>Genetic Variation</subject><subject>heart</subject><subject>Heart - parasitology</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>inflammation</subject><subject>insect vectors</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>multilocus sequence typing</subject><subject>Muscle, Skeletal - parasitology</subject><subject>Muscle, Skeletal - pathology</subject><subject>Myocardium - pathology</subject><subject>Natural isolates</subject><subject>parasites</subject><subject>Parasitic diseases</subject><subject>Protozoal diseases</subject><subject>skeletal muscle</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - classification</subject><subject>Trypanosoma cruzi - isolation & purification</subject><subject>Trypanosoma cruzi - pathogenicity</subject><subject>Trypanosomiasis</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2O0zAUhSMEYsrAK4BZILFpsJ3YSZZDBQPSSCyYkdhZN_Z16iqNi52MNLwGL8ytWn6WbPwjf_ec63uK4rXgpeBCv9uVYGeYUzwEC6XkQpZclZxXj4qVaJtqraWqHxcrzrlYN1x_uyie5byjm2yUfFpcSKnrSnbtqvj5PsQxDqQzsh63cB9iYtEzF7zHhNPMbtPDAaaY4x6YTcuPwEKOI8yYmQ3JLnQM08DCxGBiODncB8sgITBPUpstDJBJLiNkPFLzFtl1IpaebGQJhxCno-VVGsgvTPC8eOJhzPjivF8Wdx8_3G4-rW--XH_eXN2sbd3KeU0_bRtwLa-1d752qKSUQlfKC6WUE23Ve96BUrLT4JqGOwd90_e28pI3la4ui7cn3UOK3xfMs9mHbHEcYcK4ZCO4rKu667QktDuhNsWcE3pzSGEP6YEgc8zE7Mw_mZhjJoYrQ5lQ7cuzzdLv0f2p_B0CAW_OAGTKwdNwbMh_OS0IU8d-X504D9HAkIi5-0pOinJuNa1EbE4E0tjuAyaTbcDJogsJ7WxcDP_R8C9Q_rs3</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Ragone, Paula G.</creator><creator>Pérez Brandán, Cecilia</creator><creator>Padilla, Angel M.</creator><creator>Monje Rumi, Mercedes</creator><creator>Lauthier, Juan J.</creator><creator>Alberti D’Amato, Anahí M.</creator><creator>Tomasini, Nicolás</creator><creator>Cimino, Ruben O.</creator><creator>Romero, Nélida M.</creator><creator>Portelli, Marcela</creator><creator>Nasser, Julio R.</creator><creator>Basombrío, Miguel A.</creator><creator>Diosque, Patricio</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120901</creationdate><title>Biological behavior of different Trypanosoma cruzi isolates circulating in an endemic area for Chagas disease in the Gran Chaco region of Argentina</title><author>Ragone, Paula G. ; Pérez Brandán, Cecilia ; Padilla, Angel M. ; Monje Rumi, Mercedes ; Lauthier, Juan J. ; Alberti D’Amato, Anahí M. ; Tomasini, Nicolás ; Cimino, Ruben O. ; Romero, Nélida M. ; Portelli, Marcela ; Nasser, Julio R. ; Basombrío, Miguel A. ; Diosque, Patricio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-18787ad8046fdf4de52221635f1555d183bf09a55296ad770ddab7bbc3f207363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>antibodies</topic><topic>Antibodies, Protozoan - blood</topic><topic>Argentina - epidemiology</topic><topic>Biological and medical sciences</topic><topic>Biological properties</topic><topic>blood</topic><topic>Blood - parasitology</topic><topic>Chagas disease</topic><topic>Chagas Disease - epidemiology</topic><topic>Chagas Disease - immunology</topic><topic>Chagas Disease - parasitology</topic><topic>Chagas Disease - pathology</topic><topic>Disease Models, Animal</topic><topic>DNA Fingerprinting</topic><topic>DNA, Protozoan - genetics</topic><topic>electrophoresis</topic><topic>Endemic Diseases</topic><topic>Enzymes - analysis</topic><topic>General aspects</topic><topic>Genetic Variation</topic><topic>heart</topic><topic>Heart - parasitology</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>inflammation</topic><topic>insect vectors</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>multilocus sequence typing</topic><topic>Muscle, Skeletal - parasitology</topic><topic>Muscle, Skeletal - pathology</topic><topic>Myocardium - pathology</topic><topic>Natural isolates</topic><topic>parasites</topic><topic>Parasitic diseases</topic><topic>Protozoal diseases</topic><topic>skeletal muscle</topic><topic>Trypanosoma cruzi</topic><topic>Trypanosoma cruzi - classification</topic><topic>Trypanosoma cruzi - isolation & purification</topic><topic>Trypanosoma cruzi - pathogenicity</topic><topic>Trypanosomiasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ragone, Paula G.</creatorcontrib><creatorcontrib>Pérez Brandán, Cecilia</creatorcontrib><creatorcontrib>Padilla, Angel M.</creatorcontrib><creatorcontrib>Monje Rumi, Mercedes</creatorcontrib><creatorcontrib>Lauthier, Juan J.</creatorcontrib><creatorcontrib>Alberti D’Amato, Anahí M.</creatorcontrib><creatorcontrib>Tomasini, Nicolás</creatorcontrib><creatorcontrib>Cimino, Ruben O.</creatorcontrib><creatorcontrib>Romero, Nélida M.</creatorcontrib><creatorcontrib>Portelli, Marcela</creatorcontrib><creatorcontrib>Nasser, Julio R.</creatorcontrib><creatorcontrib>Basombrío, Miguel A.</creatorcontrib><creatorcontrib>Diosque, Patricio</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ragone, Paula G.</au><au>Pérez Brandán, Cecilia</au><au>Padilla, Angel M.</au><au>Monje Rumi, Mercedes</au><au>Lauthier, Juan J.</au><au>Alberti D’Amato, Anahí M.</au><au>Tomasini, Nicolás</au><au>Cimino, Ruben O.</au><au>Romero, Nélida M.</au><au>Portelli, Marcela</au><au>Nasser, Julio R.</au><au>Basombrío, Miguel A.</au><au>Diosque, Patricio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biological behavior of different Trypanosoma cruzi isolates circulating in an endemic area for Chagas disease in the Gran Chaco region of Argentina</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>123</volume><issue>3</issue><spage>196</spage><epage>201</epage><pages>196-201</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><coden>ACTRAQ</coden><abstract>This work describes the biological characterization of Trypanosoma cruzi isolates sympatrically circulating in an endemic region of Chagas disease in Chaco region, in Argentina. [Display omitted]
► The biological behavior of Trypanosoma cruzi strains is still obscure. ► Four T. cruzi DTUs were found in the study area in Argentina. ► A vector transmission model was developed to maintain T. cruzi isolates. ► Different parasite load, serological response and tissue damage among isolates was detected. ► Results let us formulate hypotheses of clinical and epidemiological importance.
The biological behavior of the different Trypanosoma cruzi strains is still unclear and the importance of exploring the relevance of these differences in natural isolates is of great significance. Herein we describe the biological behavior of four T. cruzi isolates circulating sympatrically in a restricted geographic area in Argentina endemic for Chagas Disease. These isolates were characterized as belonging to the Discrete Typing Units (DTUs) TcI, TcIII, TcV and TcVI as shown by Multilocus Enzyme Electrophoresis and Multilocus Sequence Typing. In order to study the natural behavior of the different isolates and to preserve their natural properties, we developed a vector transmission model that allows their maintenance in the laboratory. The model consisted of serial passages of these parasites between insect vectors and mice. Vector-derived parasite forms were then inoculated in C57BL/6J mice and number of parasite in peripheral blood, serological response and histological damage in acute and chronic phases of the infection were measured. Parasites from DTUs TcI, TcIII and TcVI were detected by direct fresh blood examination, while TcV parasites could only be detected by Polimerase Chain Reaction. No significant difference in the anti-T. cruzi antibody response was found during the chronic phase of infection, except for mice infected with TcV parasites where no antibodies could be detected. Histological sections showed that TcI isolate produced more damage in skeletal muscle while TcVI induced more inflammation in the heart. This work shows differential biological behavior among different parasite isolates obtained from the same cycle of transmission, permitting the opportunity to formulate future hypotheses of clinical and epidemiological importance.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>22643298</pmid><doi>10.1016/j.actatropica.2012.05.003</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| subjects | Animals antibodies Antibodies, Protozoan - blood Argentina - epidemiology Biological and medical sciences Biological properties blood Blood - parasitology Chagas disease Chagas Disease - epidemiology Chagas Disease - immunology Chagas Disease - parasitology Chagas Disease - pathology Disease Models, Animal DNA Fingerprinting DNA, Protozoan - genetics electrophoresis Endemic Diseases Enzymes - analysis General aspects Genetic Variation heart Heart - parasitology Human protozoal diseases Humans Infectious diseases inflammation insect vectors Male Medical sciences Mice Mice, Inbred C57BL multilocus sequence typing Muscle, Skeletal - parasitology Muscle, Skeletal - pathology Myocardium - pathology Natural isolates parasites Parasitic diseases Protozoal diseases skeletal muscle Trypanosoma cruzi Trypanosoma cruzi - classification Trypanosoma cruzi - isolation & purification Trypanosoma cruzi - pathogenicity Trypanosomiasis |
| title | Biological behavior of different Trypanosoma cruzi isolates circulating in an endemic area for Chagas disease in the Gran Chaco region of Argentina |
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