Endonucleolytic Inhibition Assay of DNA/Fok I Transducer as a Sensitive Platform for Homogeneous Fluorescence Detection of Small Molecule–Protein Interactions

This paper reported a novel homogeneous fluorescence assay strategy for probing small molecule–protein interactions based on endonucleolytic inhibition of a DNA/Fok I transducer. The transducer could cyclically cleave fluorescence-quenched probes to yield activated fluorescence signal, while protein...

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Veröffentlicht in:	Analytical chemistry (Washington) 2012-07, Vol.84 (13), p.5708-5715
Hauptverfasser:	Zhen, Zhen, Tang, Li-Juan, Lin, Jian, Jiang, Jian-Hui, Yu, Ru-Qin, Xiong, Xiangling, Tan, Weihong
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Binding, Competitive Bioassays Biological and medical sciences Chemistry Deoxyribonucleases, Type II Site-Specific - metabolism Deoxyribonucleic acid DNA DNA - metabolism Exact sciences and technology Fluorescence Fluorescent Dyes - metabolism Fundamental and applied biological sciences. Psychology Humans Interactions. Associations Intermolecular phenomena Ligands Molecular biophysics Molecules Protein Binding Proteins Proteins - metabolism Spectrometric and optical methods Spectrometry, Fluorescence - methods Transducers
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