Adsorption of Enamel Matrix Proteins to a Bovine‐Derived Bone Grafting Material and Its Regulation of Cell Adhesion, Proliferation, and Differentiation
Background: The use of various combinations of enamel matrix derivative (EMD) and grafting materials has been shown to promote periodontal wound healing/regeneration. However, the downstream cellular behavior of periodontal ligament (PDL) cells and osteoblasts has not yet been studied. Furthermore,...
Gespeichert in:
| Veröffentlicht in: | Journal of periodontology (1970) 2012-07, Vol.83 (7), p.936-947 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 947 |
|---|---|
| container_issue | 7 |
| container_start_page | 936 |
| container_title | Journal of periodontology (1970) |
| container_volume | 83 |
| creator | Miron, Richard J. D. Bosshardt, Dieter Hedbom, Erik Zhang, Yufeng Haenni, Beat Buser, Daniel Sculean, Anton |
| description | Background: The use of various combinations of enamel matrix derivative (EMD) and grafting materials has been shown to promote periodontal wound healing/regeneration. However, the downstream cellular behavior of periodontal ligament (PDL) cells and osteoblasts has not yet been studied. Furthermore, it is unknown to what extent the bleeding during regenerative surgery may influence the adsorption of exogenous proteins to the surface of bone grafting materials and the subsequent cellular behavior. In the present study, the aim is to test EMD adsorption to the surface of natural bone mineral (NBM) particles in the presence of blood and determine the effect of EMD coating to NBM particles on downstream cellular pathways, such as adhesion, proliferation, and differentiation of primary human osteoblasts and PDL cells.
Methods: NBM particles were precoated in various settings with EMD or human blood and analyzed for protein adsorption patterns via fluorescent imaging and high‐resolution immunocytochemistry with an anti‐EMD antibody. Cell attachment and cell proliferation were quantified using fluorescent double‐stranded DNA‐binding dye. Cell differentiation was analyzed using real‐time polymerase chain reaction for genes encoding runt‐related transcription factor 2, alkaline phosphatase (ALP), osteocalcin (OC), and collagen1α1 (COL1A1), and mineralization was assessed using red dye staining.
Results: Analysis of cell attachment and cell proliferation revealed significantly higher osteoblast and PDL cell attachment on EMD‐coated surfaces when compared with control and blood‐coated surfaces. EMD also stimulated release of growth factors and cytokines, including bone morphogenetic protein 2 and transforming growth factor β1. Moreover, there were significantly higher mRNA levels of osteoblast differentiation markers, including COL1A1, ALP, and OC, in osteoblasts and PDL cells cultured on EMD‐coated NBM particles.
Conclusion: The present results suggest that 1) EMD enhances osteoblast and PDL cell attachment, proliferation, and differentiation on NBM particles, and 2) blood contamination of the grafting material before mixing with EMD may inhibit EMD adsorption. |
| doi_str_mv | 10.1902/jop.2011.110480 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1024096170</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1024096170</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4396-972cf32991594b694db738010455e25bec0631966f91fb982ae359940d408c383</originalsourceid><addsrcrecordid>eNqFkc1u1DAUhS0EotPCmh3yBolFM73-iRMvh-lQioqoKlhHTnJdXGXsqZ0pdNdHYMvr8SR4fgpLVraPv3Ou5UPIKwZTpoGf3ITVlANjU8ZA1vCETJiWohCqgqdkAsB5IaTmB-QwpZt8ZFLAc3LA84YJBRPya9anEFejC54GSxfeLHGgn8wY3Q96GcOIzic6Bmrou3DnPP5--HmK0d1hnwWP9CwaOzp_vfFk3QzU-J6ej4le4fV6MI_JcxwGOuu_YcrC8SZ6cBbj9v546zl1NgvoR7cVX5Bn1gwJX-7XI_L1_eLL_ENx8fnsfD67KDoptCp0xTsruNas1LJVWvZtJWrI31GWyMsWO1CCaaWsZrbVNTcoSq0l9BLqTtTiiLzd5a5iuF1jGpulS11-rfEY1qlhwCVoxSrI6MkO7WJIKaJtVtEtTbzPULPpo8l9NJs-ml0f2fF6H75ul9j_5R8LyMCbPWBSZwYbje9c-scpJlildebKHffdDXj_v7nNx8vFFWihxB8_nqQp</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1024096170</pqid></control><display><type>article</type><title>Adsorption of Enamel Matrix Proteins to a Bovine‐Derived Bone Grafting Material and Its Regulation of Cell Adhesion, Proliferation, and Differentiation</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Miron, Richard J. ; D. Bosshardt, Dieter ; Hedbom, Erik ; Zhang, Yufeng ; Haenni, Beat ; Buser, Daniel ; Sculean, Anton</creator><creatorcontrib>Miron, Richard J. ; D. Bosshardt, Dieter ; Hedbom, Erik ; Zhang, Yufeng ; Haenni, Beat ; Buser, Daniel ; Sculean, Anton</creatorcontrib><description>Background: The use of various combinations of enamel matrix derivative (EMD) and grafting materials has been shown to promote periodontal wound healing/regeneration. However, the downstream cellular behavior of periodontal ligament (PDL) cells and osteoblasts has not yet been studied. Furthermore, it is unknown to what extent the bleeding during regenerative surgery may influence the adsorption of exogenous proteins to the surface of bone grafting materials and the subsequent cellular behavior. In the present study, the aim is to test EMD adsorption to the surface of natural bone mineral (NBM) particles in the presence of blood and determine the effect of EMD coating to NBM particles on downstream cellular pathways, such as adhesion, proliferation, and differentiation of primary human osteoblasts and PDL cells.
Methods: NBM particles were precoated in various settings with EMD or human blood and analyzed for protein adsorption patterns via fluorescent imaging and high‐resolution immunocytochemistry with an anti‐EMD antibody. Cell attachment and cell proliferation were quantified using fluorescent double‐stranded DNA‐binding dye. Cell differentiation was analyzed using real‐time polymerase chain reaction for genes encoding runt‐related transcription factor 2, alkaline phosphatase (ALP), osteocalcin (OC), and collagen1α1 (COL1A1), and mineralization was assessed using red dye staining.
Results: Analysis of cell attachment and cell proliferation revealed significantly higher osteoblast and PDL cell attachment on EMD‐coated surfaces when compared with control and blood‐coated surfaces. EMD also stimulated release of growth factors and cytokines, including bone morphogenetic protein 2 and transforming growth factor β1. Moreover, there were significantly higher mRNA levels of osteoblast differentiation markers, including COL1A1, ALP, and OC, in osteoblasts and PDL cells cultured on EMD‐coated NBM particles.
Conclusion: The present results suggest that 1) EMD enhances osteoblast and PDL cell attachment, proliferation, and differentiation on NBM particles, and 2) blood contamination of the grafting material before mixing with EMD may inhibit EMD adsorption.</description><identifier>ISSN: 0022-3492</identifier><identifier>EISSN: 1943-3670</identifier><identifier>DOI: 10.1902/jop.2011.110480</identifier><identifier>PMID: 22141360</identifier><language>eng</language><publisher>Chicago, IL: American Academy of Periodontology</publisher><subject>Adsorption ; Alkaline Phosphatase - analysis ; Animals ; Anthraquinones ; Biocompatible materials ; Biological and medical sciences ; Blood ; blood proteins ; Bone Morphogenetic Protein 2 - analysis ; bone regeneration ; Bone Substitutes - chemistry ; Calcification, Physiologic - drug effects ; Cattle ; Cell Adhesion - drug effects ; Cell Culture Techniques ; Cell Differentiation - drug effects ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Cell Proliferation - drug effects ; Coated Materials, Biocompatible - chemistry ; Coated Materials, Biocompatible - pharmacology ; Collagen Type I - analysis ; Coloring Agents ; Core Binding Factor Alpha 1 Subunit - analysis ; Dental Enamel Proteins - chemistry ; Dental Enamel Proteins - pharmacology ; Dentistry ; enamel matrix proteins ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; guided tissue regeneration ; Humans ; Medical sciences ; Microscopy, Electron, Scanning ; Minerals - chemistry ; Molecular and cellular biology ; Osteoblasts - drug effects ; Osteoblasts - physiology ; Osteocalcin - analysis ; Otorhinolaryngology. Stomatology ; periodontal ; Periodontal Ligament - cytology ; Periodontal Ligament - drug effects ; Real-Time Polymerase Chain Reaction ; tissue engineering ; Transforming Growth Factor beta1 - analysis</subject><ispartof>Journal of periodontology (1970), 2012-07, Vol.83 (7), p.936-947</ispartof><rights>2012 American Academy of Periodontology</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4396-972cf32991594b694db738010455e25bec0631966f91fb982ae359940d408c383</citedby><cites>FETCH-LOGICAL-c4396-972cf32991594b694db738010455e25bec0631966f91fb982ae359940d408c383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1902%2Fjop.2011.110480$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1902%2Fjop.2011.110480$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26131799$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22141360$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miron, Richard J.</creatorcontrib><creatorcontrib>D. Bosshardt, Dieter</creatorcontrib><creatorcontrib>Hedbom, Erik</creatorcontrib><creatorcontrib>Zhang, Yufeng</creatorcontrib><creatorcontrib>Haenni, Beat</creatorcontrib><creatorcontrib>Buser, Daniel</creatorcontrib><creatorcontrib>Sculean, Anton</creatorcontrib><title>Adsorption of Enamel Matrix Proteins to a Bovine‐Derived Bone Grafting Material and Its Regulation of Cell Adhesion, Proliferation, and Differentiation</title><title>Journal of periodontology (1970)</title><addtitle>J Periodontol</addtitle><description>Background: The use of various combinations of enamel matrix derivative (EMD) and grafting materials has been shown to promote periodontal wound healing/regeneration. However, the downstream cellular behavior of periodontal ligament (PDL) cells and osteoblasts has not yet been studied. Furthermore, it is unknown to what extent the bleeding during regenerative surgery may influence the adsorption of exogenous proteins to the surface of bone grafting materials and the subsequent cellular behavior. In the present study, the aim is to test EMD adsorption to the surface of natural bone mineral (NBM) particles in the presence of blood and determine the effect of EMD coating to NBM particles on downstream cellular pathways, such as adhesion, proliferation, and differentiation of primary human osteoblasts and PDL cells.
Methods: NBM particles were precoated in various settings with EMD or human blood and analyzed for protein adsorption patterns via fluorescent imaging and high‐resolution immunocytochemistry with an anti‐EMD antibody. Cell attachment and cell proliferation were quantified using fluorescent double‐stranded DNA‐binding dye. Cell differentiation was analyzed using real‐time polymerase chain reaction for genes encoding runt‐related transcription factor 2, alkaline phosphatase (ALP), osteocalcin (OC), and collagen1α1 (COL1A1), and mineralization was assessed using red dye staining.
Results: Analysis of cell attachment and cell proliferation revealed significantly higher osteoblast and PDL cell attachment on EMD‐coated surfaces when compared with control and blood‐coated surfaces. EMD also stimulated release of growth factors and cytokines, including bone morphogenetic protein 2 and transforming growth factor β1. Moreover, there were significantly higher mRNA levels of osteoblast differentiation markers, including COL1A1, ALP, and OC, in osteoblasts and PDL cells cultured on EMD‐coated NBM particles.
Conclusion: The present results suggest that 1) EMD enhances osteoblast and PDL cell attachment, proliferation, and differentiation on NBM particles, and 2) blood contamination of the grafting material before mixing with EMD may inhibit EMD adsorption.</description><subject>Adsorption</subject><subject>Alkaline Phosphatase - analysis</subject><subject>Animals</subject><subject>Anthraquinones</subject><subject>Biocompatible materials</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>blood proteins</subject><subject>Bone Morphogenetic Protein 2 - analysis</subject><subject>bone regeneration</subject><subject>Bone Substitutes - chemistry</subject><subject>Calcification, Physiologic - drug effects</subject><subject>Cattle</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Cell Proliferation - drug effects</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Coated Materials, Biocompatible - pharmacology</subject><subject>Collagen Type I - analysis</subject><subject>Coloring Agents</subject><subject>Core Binding Factor Alpha 1 Subunit - analysis</subject><subject>Dental Enamel Proteins - chemistry</subject><subject>Dental Enamel Proteins - pharmacology</subject><subject>Dentistry</subject><subject>enamel matrix proteins</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>guided tissue regeneration</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Scanning</subject><subject>Minerals - chemistry</subject><subject>Molecular and cellular biology</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - physiology</subject><subject>Osteocalcin - analysis</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>periodontal</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - drug effects</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>tissue engineering</subject><subject>Transforming Growth Factor beta1 - analysis</subject><issn>0022-3492</issn><issn>1943-3670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EotPCmh3yBolFM73-iRMvh-lQioqoKlhHTnJdXGXsqZ0pdNdHYMvr8SR4fgpLVraPv3Ou5UPIKwZTpoGf3ITVlANjU8ZA1vCETJiWohCqgqdkAsB5IaTmB-QwpZt8ZFLAc3LA84YJBRPya9anEFejC54GSxfeLHGgn8wY3Q96GcOIzic6Bmrou3DnPP5--HmK0d1hnwWP9CwaOzp_vfFk3QzU-J6ej4le4fV6MI_JcxwGOuu_YcrC8SZ6cBbj9v546zl1NgvoR7cVX5Bn1gwJX-7XI_L1_eLL_ENx8fnsfD67KDoptCp0xTsruNas1LJVWvZtJWrI31GWyMsWO1CCaaWsZrbVNTcoSq0l9BLqTtTiiLzd5a5iuF1jGpulS11-rfEY1qlhwCVoxSrI6MkO7WJIKaJtVtEtTbzPULPpo8l9NJs-ml0f2fF6H75ul9j_5R8LyMCbPWBSZwYbje9c-scpJlildebKHffdDXj_v7nNx8vFFWihxB8_nqQp</recordid><startdate>201207</startdate><enddate>201207</enddate><creator>Miron, Richard J.</creator><creator>D. Bosshardt, Dieter</creator><creator>Hedbom, Erik</creator><creator>Zhang, Yufeng</creator><creator>Haenni, Beat</creator><creator>Buser, Daniel</creator><creator>Sculean, Anton</creator><general>American Academy of Periodontology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201207</creationdate><title>Adsorption of Enamel Matrix Proteins to a Bovine‐Derived Bone Grafting Material and Its Regulation of Cell Adhesion, Proliferation, and Differentiation</title><author>Miron, Richard J. ; D. Bosshardt, Dieter ; Hedbom, Erik ; Zhang, Yufeng ; Haenni, Beat ; Buser, Daniel ; Sculean, Anton</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4396-972cf32991594b694db738010455e25bec0631966f91fb982ae359940d408c383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adsorption</topic><topic>Alkaline Phosphatase - analysis</topic><topic>Animals</topic><topic>Anthraquinones</topic><topic>Biocompatible materials</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>blood proteins</topic><topic>Bone Morphogenetic Protein 2 - analysis</topic><topic>bone regeneration</topic><topic>Bone Substitutes - chemistry</topic><topic>Calcification, Physiologic - drug effects</topic><topic>Cattle</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Cell Proliferation - drug effects</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Coated Materials, Biocompatible - pharmacology</topic><topic>Collagen Type I - analysis</topic><topic>Coloring Agents</topic><topic>Core Binding Factor Alpha 1 Subunit - analysis</topic><topic>Dental Enamel Proteins - chemistry</topic><topic>Dental Enamel Proteins - pharmacology</topic><topic>Dentistry</topic><topic>enamel matrix proteins</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>guided tissue regeneration</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microscopy, Electron, Scanning</topic><topic>Minerals - chemistry</topic><topic>Molecular and cellular biology</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - physiology</topic><topic>Osteocalcin - analysis</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>periodontal</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - drug effects</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>tissue engineering</topic><topic>Transforming Growth Factor beta1 - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miron, Richard J.</creatorcontrib><creatorcontrib>D. Bosshardt, Dieter</creatorcontrib><creatorcontrib>Hedbom, Erik</creatorcontrib><creatorcontrib>Zhang, Yufeng</creatorcontrib><creatorcontrib>Haenni, Beat</creatorcontrib><creatorcontrib>Buser, Daniel</creatorcontrib><creatorcontrib>Sculean, Anton</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontology (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miron, Richard J.</au><au>D. Bosshardt, Dieter</au><au>Hedbom, Erik</au><au>Zhang, Yufeng</au><au>Haenni, Beat</au><au>Buser, Daniel</au><au>Sculean, Anton</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adsorption of Enamel Matrix Proteins to a Bovine‐Derived Bone Grafting Material and Its Regulation of Cell Adhesion, Proliferation, and Differentiation</atitle><jtitle>Journal of periodontology (1970)</jtitle><addtitle>J Periodontol</addtitle><date>2012-07</date><risdate>2012</risdate><volume>83</volume><issue>7</issue><spage>936</spage><epage>947</epage><pages>936-947</pages><issn>0022-3492</issn><eissn>1943-3670</eissn><abstract>Background: The use of various combinations of enamel matrix derivative (EMD) and grafting materials has been shown to promote periodontal wound healing/regeneration. However, the downstream cellular behavior of periodontal ligament (PDL) cells and osteoblasts has not yet been studied. Furthermore, it is unknown to what extent the bleeding during regenerative surgery may influence the adsorption of exogenous proteins to the surface of bone grafting materials and the subsequent cellular behavior. In the present study, the aim is to test EMD adsorption to the surface of natural bone mineral (NBM) particles in the presence of blood and determine the effect of EMD coating to NBM particles on downstream cellular pathways, such as adhesion, proliferation, and differentiation of primary human osteoblasts and PDL cells.
Methods: NBM particles were precoated in various settings with EMD or human blood and analyzed for protein adsorption patterns via fluorescent imaging and high‐resolution immunocytochemistry with an anti‐EMD antibody. Cell attachment and cell proliferation were quantified using fluorescent double‐stranded DNA‐binding dye. Cell differentiation was analyzed using real‐time polymerase chain reaction for genes encoding runt‐related transcription factor 2, alkaline phosphatase (ALP), osteocalcin (OC), and collagen1α1 (COL1A1), and mineralization was assessed using red dye staining.
Results: Analysis of cell attachment and cell proliferation revealed significantly higher osteoblast and PDL cell attachment on EMD‐coated surfaces when compared with control and blood‐coated surfaces. EMD also stimulated release of growth factors and cytokines, including bone morphogenetic protein 2 and transforming growth factor β1. Moreover, there were significantly higher mRNA levels of osteoblast differentiation markers, including COL1A1, ALP, and OC, in osteoblasts and PDL cells cultured on EMD‐coated NBM particles.
Conclusion: The present results suggest that 1) EMD enhances osteoblast and PDL cell attachment, proliferation, and differentiation on NBM particles, and 2) blood contamination of the grafting material before mixing with EMD may inhibit EMD adsorption.</abstract><cop>Chicago, IL</cop><pub>American Academy of Periodontology</pub><pmid>22141360</pmid><doi>10.1902/jop.2011.110480</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3492 |
| ispartof | Journal of periodontology (1970), 2012-07, Vol.83 (7), p.936-947 |
| issn | 0022-3492 1943-3670 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1024096170 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Adsorption Alkaline Phosphatase - analysis Animals Anthraquinones Biocompatible materials Biological and medical sciences Blood blood proteins Bone Morphogenetic Protein 2 - analysis bone regeneration Bone Substitutes - chemistry Calcification, Physiologic - drug effects Cattle Cell Adhesion - drug effects Cell Culture Techniques Cell Differentiation - drug effects Cell differentiation, maturation, development, hematopoiesis Cell physiology Cell Proliferation - drug effects Coated Materials, Biocompatible - chemistry Coated Materials, Biocompatible - pharmacology Collagen Type I - analysis Coloring Agents Core Binding Factor Alpha 1 Subunit - analysis Dental Enamel Proteins - chemistry Dental Enamel Proteins - pharmacology Dentistry enamel matrix proteins Fluorescent Dyes Fundamental and applied biological sciences. Psychology guided tissue regeneration Humans Medical sciences Microscopy, Electron, Scanning Minerals - chemistry Molecular and cellular biology Osteoblasts - drug effects Osteoblasts - physiology Osteocalcin - analysis Otorhinolaryngology. Stomatology periodontal Periodontal Ligament - cytology Periodontal Ligament - drug effects Real-Time Polymerase Chain Reaction tissue engineering Transforming Growth Factor beta1 - analysis |
| title | Adsorption of Enamel Matrix Proteins to a Bovine‐Derived Bone Grafting Material and Its Regulation of Cell Adhesion, Proliferation, and Differentiation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T06%3A51%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Adsorption%20of%20Enamel%20Matrix%20Proteins%20to%20a%20Bovine%E2%80%90Derived%20Bone%20Grafting%20Material%20and%20Its%20Regulation%20of%20Cell%20Adhesion,%20Proliferation,%20and%20Differentiation&rft.jtitle=Journal%20of%20periodontology%20(1970)&rft.au=Miron,%20Richard%20J.&rft.date=2012-07&rft.volume=83&rft.issue=7&rft.spage=936&rft.epage=947&rft.pages=936-947&rft.issn=0022-3492&rft.eissn=1943-3670&rft_id=info:doi/10.1902/jop.2011.110480&rft_dat=%3Cproquest_cross%3E1024096170%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1024096170&rft_id=info:pmid/22141360&rfr_iscdi=true |