Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis

Sample handling procedures including protein digestion, glycopeptide enrichment, and deglycosylation have significant impact on the performance of glycoproteome analysis. Several glycoproteomic analysis systems were developed to integrate some of these sample preparation procedures. However, no micr...

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Veröffentlicht in:Analytical chemistry (Washington) 2012-06, Vol.84 (11), p.5146-5153
Hauptverfasser: Zhu, Jun, Wang, Fangjun, Chen, Rui, Cheng, Kai, Xu, Bo, Guo, Zhimou, Liang, Xinmiao, Ye, Mingliang, Zou, Hanfa
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Sprache:eng
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