Detection and molecular characterization of avian Plasmodium from mosquitoes in central Turkey
Assessing vector–parasite relationship is important in understanding the emergence of vector–borne diseases and the evolution of parasite diversity. This study investigates avian Plasmodium parasites in mosquitoes collected from Kayseri province in Central Anatolian, Turkey and determines the haemos...
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description | Assessing vector–parasite relationship is important in understanding the emergence of vector–borne diseases and the evolution of parasite diversity. This study investigates avian Plasmodium parasites in mosquitoes collected from Kayseri province in Central Anatolian, Turkey and determines the haemosporidian parasite lineages from these mosquito species. A total of 6153 female mosquitos from 6 species were collected from 46 sites during June–August of 2008 and 2009. Each mosquito's head–thorax and abdomen were separated, categorized with respect to species and collection area and pooled for DNA extraction. A total of 1198 genomic DNA pools (599 thorax–head, 599 abdomen) were constituted of which128 pools (59 thorax–head, 69 abdomen) were positive for avian haemosporidian parasites (Plasmodium and Haemoproteus) by Nested-PCR analysis. Culex pipens, Aedes vexans, Culex theileri and Culiseta annulata were positive with minimum infection rates (MIRs) of 16.22 and 18.15, 4.72 and 5.98, 5.18 and 10.36, 10.64 and 10.64 in their thorax–head and abdomen parts, respectively. No avian haemosporidian DNA was detected from Culex hortensis and Anopheles maculipennis. Phylogenetic analyses of the partial cytb gene of avian haemosporidian mt-DNA from 13 positive pools revealed that 11 lineages in four phylogenic groups were Plasmodium and the other two were Haemoproteus. Our results suggest that Cx. pipiens could probably be the major vector of avian Plasmodium in Central Turkey. This is the first report of molecular detection and characterization of avian Plasmodium lineages from mosquitoes in Turkey. |
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This study investigates avian Plasmodium parasites in mosquitoes collected from Kayseri province in Central Anatolian, Turkey and determines the haemosporidian parasite lineages from these mosquito species. A total of 6153 female mosquitos from 6 species were collected from 46 sites during June–August of 2008 and 2009. Each mosquito's head–thorax and abdomen were separated, categorized with respect to species and collection area and pooled for DNA extraction. A total of 1198 genomic DNA pools (599 thorax–head, 599 abdomen) were constituted of which128 pools (59 thorax–head, 69 abdomen) were positive for avian haemosporidian parasites (Plasmodium and Haemoproteus) by Nested-PCR analysis. Culex pipens, Aedes vexans, Culex theileri and Culiseta annulata were positive with minimum infection rates (MIRs) of 16.22 and 18.15, 4.72 and 5.98, 5.18 and 10.36, 10.64 and 10.64 in their thorax–head and abdomen parts, respectively. No avian haemosporidian DNA was detected from Culex hortensis and Anopheles maculipennis. Phylogenetic analyses of the partial cytb gene of avian haemosporidian mt-DNA from 13 positive pools revealed that 11 lineages in four phylogenic groups were Plasmodium and the other two were Haemoproteus. Our results suggest that Cx. pipiens could probably be the major vector of avian Plasmodium in Central Turkey. This is the first report of molecular detection and characterization of avian Plasmodium lineages from mosquitoes in Turkey.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2012.02.012</identifier><identifier>PMID: 22455723</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>abdomen ; Aedes vexans ; Animals ; Anopheles maculipennis ; Avian ; Bird Diseases - parasitology ; Birds ; Culex theileri ; Culicidae - classification ; Culicidae - parasitology ; Culiseta ; DNA ; DNA, Protozoan - classification ; DNA, Protozoan - genetics ; Female ; Haemoproteus ; Malaria - parasitology ; Malaria - veterinary ; Molecular characterization ; parasites ; Phylogeny ; Plasmodium ; Plasmodium - classification ; Plasmodium - genetics ; Plasmodium - isolation & purification ; Plasmodium spp ; Species Specificity ; Time Factors ; Turkey ; Turkey - epidemiology ; Vector ; vector-borne diseases</subject><ispartof>Veterinary parasitology, 2012-08, Vol.188 (1-2), p.179-184</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-ddfd59dc1886e5e6560abfedbdab7801eaf6a0a457dff3d90d03f9010f2499d63</citedby><cites>FETCH-LOGICAL-c386t-ddfd59dc1886e5e6560abfedbdab7801eaf6a0a457dff3d90d03f9010f2499d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetpar.2012.02.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22455723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inci, A.</creatorcontrib><creatorcontrib>Yildirim, A.</creatorcontrib><creatorcontrib>Njabo, K.Y.</creatorcontrib><creatorcontrib>Duzlu, O.</creatorcontrib><creatorcontrib>Biskin, Z.</creatorcontrib><creatorcontrib>Ciloglu, A.</creatorcontrib><title>Detection and molecular characterization of avian Plasmodium from mosquitoes in central Turkey</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>Assessing vector–parasite relationship is important in understanding the emergence of vector–borne diseases and the evolution of parasite diversity. This study investigates avian Plasmodium parasites in mosquitoes collected from Kayseri province in Central Anatolian, Turkey and determines the haemosporidian parasite lineages from these mosquito species. A total of 6153 female mosquitos from 6 species were collected from 46 sites during June–August of 2008 and 2009. Each mosquito's head–thorax and abdomen were separated, categorized with respect to species and collection area and pooled for DNA extraction. A total of 1198 genomic DNA pools (599 thorax–head, 599 abdomen) were constituted of which128 pools (59 thorax–head, 69 abdomen) were positive for avian haemosporidian parasites (Plasmodium and Haemoproteus) by Nested-PCR analysis. Culex pipens, Aedes vexans, Culex theileri and Culiseta annulata were positive with minimum infection rates (MIRs) of 16.22 and 18.15, 4.72 and 5.98, 5.18 and 10.36, 10.64 and 10.64 in their thorax–head and abdomen parts, respectively. No avian haemosporidian DNA was detected from Culex hortensis and Anopheles maculipennis. Phylogenetic analyses of the partial cytb gene of avian haemosporidian mt-DNA from 13 positive pools revealed that 11 lineages in four phylogenic groups were Plasmodium and the other two were Haemoproteus. Our results suggest that Cx. pipiens could probably be the major vector of avian Plasmodium in Central Turkey. This is the first report of molecular detection and characterization of avian Plasmodium lineages from mosquitoes in Turkey.</description><subject>abdomen</subject><subject>Aedes vexans</subject><subject>Animals</subject><subject>Anopheles maculipennis</subject><subject>Avian</subject><subject>Bird Diseases - parasitology</subject><subject>Birds</subject><subject>Culex theileri</subject><subject>Culicidae - classification</subject><subject>Culicidae - parasitology</subject><subject>Culiseta</subject><subject>DNA</subject><subject>DNA, Protozoan - classification</subject><subject>DNA, Protozoan - genetics</subject><subject>Female</subject><subject>Haemoproteus</subject><subject>Malaria - parasitology</subject><subject>Malaria - veterinary</subject><subject>Molecular characterization</subject><subject>parasites</subject><subject>Phylogeny</subject><subject>Plasmodium</subject><subject>Plasmodium - classification</subject><subject>Plasmodium - genetics</subject><subject>Plasmodium - isolation & purification</subject><subject>Plasmodium spp</subject><subject>Species Specificity</subject><subject>Time Factors</subject><subject>Turkey</subject><subject>Turkey - epidemiology</subject><subject>Vector</subject><subject>vector-borne diseases</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAQhi0EotvCGyDwkUuWseM4yQUJtVCQKoFEe8WatcfgJYm3drJSeXpcUjgijeTDfP-M9Q1jLwRsBQj9Zr890nzAtJUg5BZKCfmIbUTX1pVsGnjMNlCDqhSI9oSd5rwHAAW6fcpOpFRN08p6w75d0Ex2DnHiODk-xoHsMmDi9gcmtDOl8Av_tKPneAw48S8D5jG6sIzcpziWTL5dwhwp8zBxS9OccODXS_pJd8_YE49DpucP7xm7-fD--vxjdfX58tP5u6vK1p2eK-e8a3pnRddpakg3GnDnye0c7toOBKHXCKia1nlfux4c1L4HAV6qvne6PmOv17mHFG8XyrMZQ7Y0DDhRXLIRIGvZa5BQULWiNsWcE3lzSGHEdFcgc2_W7M1q1tybNVBKyBJ7-bBh2Y3k_oX-qizAqxXwGA1-TyGbm69lgoJyAGhVW4i3K0HFxDFQMtkGmiy5kMoNjIvh_3_4DSWTl18</recordid><startdate>20120813</startdate><enddate>20120813</enddate><creator>Inci, A.</creator><creator>Yildirim, A.</creator><creator>Njabo, K.Y.</creator><creator>Duzlu, O.</creator><creator>Biskin, Z.</creator><creator>Ciloglu, A.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120813</creationdate><title>Detection and molecular characterization of avian Plasmodium from mosquitoes in central Turkey</title><author>Inci, A. ; Yildirim, A. ; Njabo, K.Y. ; Duzlu, O. ; Biskin, Z. ; Ciloglu, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-ddfd59dc1886e5e6560abfedbdab7801eaf6a0a457dff3d90d03f9010f2499d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>abdomen</topic><topic>Aedes vexans</topic><topic>Animals</topic><topic>Anopheles maculipennis</topic><topic>Avian</topic><topic>Bird Diseases - parasitology</topic><topic>Birds</topic><topic>Culex theileri</topic><topic>Culicidae - classification</topic><topic>Culicidae - parasitology</topic><topic>Culiseta</topic><topic>DNA</topic><topic>DNA, Protozoan - classification</topic><topic>DNA, Protozoan - genetics</topic><topic>Female</topic><topic>Haemoproteus</topic><topic>Malaria - parasitology</topic><topic>Malaria - veterinary</topic><topic>Molecular characterization</topic><topic>parasites</topic><topic>Phylogeny</topic><topic>Plasmodium</topic><topic>Plasmodium - classification</topic><topic>Plasmodium - genetics</topic><topic>Plasmodium - isolation & purification</topic><topic>Plasmodium spp</topic><topic>Species Specificity</topic><topic>Time Factors</topic><topic>Turkey</topic><topic>Turkey - epidemiology</topic><topic>Vector</topic><topic>vector-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inci, A.</creatorcontrib><creatorcontrib>Yildirim, A.</creatorcontrib><creatorcontrib>Njabo, K.Y.</creatorcontrib><creatorcontrib>Duzlu, O.</creatorcontrib><creatorcontrib>Biskin, Z.</creatorcontrib><creatorcontrib>Ciloglu, A.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inci, A.</au><au>Yildirim, A.</au><au>Njabo, K.Y.</au><au>Duzlu, O.</au><au>Biskin, Z.</au><au>Ciloglu, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and molecular characterization of avian Plasmodium from mosquitoes in central Turkey</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2012-08-13</date><risdate>2012</risdate><volume>188</volume><issue>1-2</issue><spage>179</spage><epage>184</epage><pages>179-184</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>Assessing vector–parasite relationship is important in understanding the emergence of vector–borne diseases and the evolution of parasite diversity. This study investigates avian Plasmodium parasites in mosquitoes collected from Kayseri province in Central Anatolian, Turkey and determines the haemosporidian parasite lineages from these mosquito species. A total of 6153 female mosquitos from 6 species were collected from 46 sites during June–August of 2008 and 2009. Each mosquito's head–thorax and abdomen were separated, categorized with respect to species and collection area and pooled for DNA extraction. A total of 1198 genomic DNA pools (599 thorax–head, 599 abdomen) were constituted of which128 pools (59 thorax–head, 69 abdomen) were positive for avian haemosporidian parasites (Plasmodium and Haemoproteus) by Nested-PCR analysis. Culex pipens, Aedes vexans, Culex theileri and Culiseta annulata were positive with minimum infection rates (MIRs) of 16.22 and 18.15, 4.72 and 5.98, 5.18 and 10.36, 10.64 and 10.64 in their thorax–head and abdomen parts, respectively. No avian haemosporidian DNA was detected from Culex hortensis and Anopheles maculipennis. Phylogenetic analyses of the partial cytb gene of avian haemosporidian mt-DNA from 13 positive pools revealed that 11 lineages in four phylogenic groups were Plasmodium and the other two were Haemoproteus. Our results suggest that Cx. pipiens could probably be the major vector of avian Plasmodium in Central Turkey. This is the first report of molecular detection and characterization of avian Plasmodium lineages from mosquitoes in Turkey.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22455723</pmid><doi>10.1016/j.vetpar.2012.02.012</doi><tpages>6</tpages></addata></record> |
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subjects | abdomen Aedes vexans Animals Anopheles maculipennis Avian Bird Diseases - parasitology Birds Culex theileri Culicidae - classification Culicidae - parasitology Culiseta DNA DNA, Protozoan - classification DNA, Protozoan - genetics Female Haemoproteus Malaria - parasitology Malaria - veterinary Molecular characterization parasites Phylogeny Plasmodium Plasmodium - classification Plasmodium - genetics Plasmodium - isolation & purification Plasmodium spp Species Specificity Time Factors Turkey Turkey - epidemiology Vector vector-borne diseases |
title | Detection and molecular characterization of avian Plasmodium from mosquitoes in central Turkey |
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