Matrix Metalloproteinases (MMPs) Activity in Cultured Canine Bone Marrow Stromal Cells (BMSCs)
Autologous bone marrow stromal cells (BMSCs) infusion therapy improves the hepatic fibrosis. To investigate the mechanism of remission, we evaluated the matrix metalloproteinase (MMP)-2 and -9 activity in canine BMSCs and the effect of pro-inflammatory cytokines on their expression. The activity and...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2012, Vol.74(5), pp.633-636 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | HARAGUCHI, Tomoya TANI, Kenji KOGA, Masato ODA, Yasutaka ITAMOTO, Kazuhito YAMAMOTO, Naoki TERAI, Shuji SAKAIDA, Isao NAKAZAWA, Hiroshi TAURA, Yasuho |
| description | Autologous bone marrow stromal cells (BMSCs) infusion therapy improves the hepatic fibrosis. To investigate the mechanism of remission, we evaluated the matrix metalloproteinase (MMP)-2 and -9 activity in canine BMSCs and the effect of pro-inflammatory cytokines on their expression. The activity and the gene expression of MMPs were analyzed by gelatin zymography and quantitative RT-PCR, respectively. The specific gelatinase bands were indicative effect of MMP-2 and -9 in canine BMSCs. MMP-2 expression seemed to be increased by TNF-α and IL-1β while MMP-9 was enhanced by TNF-α and IL-6. These results suggested that remissive effect on liver fibrosis might be partly attributable to the MMP-2 and -9 activity in BMSCs under the inflammatory condition. |
| doi_str_mv | 10.1292/jvms.11-0395 |
| format | Article |
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To investigate the mechanism of remission, we evaluated the matrix metalloproteinase (MMP)-2 and -9 activity in canine BMSCs and the effect of pro-inflammatory cytokines on their expression. The activity and the gene expression of MMPs were analyzed by gelatin zymography and quantitative RT-PCR, respectively. The specific gelatinase bands were indicative effect of MMP-2 and -9 in canine BMSCs. MMP-2 expression seemed to be increased by TNF-α and IL-1β while MMP-9 was enhanced by TNF-α and IL-6. These results suggested that remissive effect on liver fibrosis might be partly attributable to the MMP-2 and -9 activity in BMSCs under the inflammatory condition.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.11-0395</identifier><identifier>PMID: 22167104</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; Bone Marrow Cells - enzymology ; bone marrow stromal cell (BMSC) ; canine ; Cells, Cultured ; culture ; Dogs - metabolism ; Gene Expression Regulation, Enzymologic - physiology ; inflammatory cytokines ; Interleukin-1beta - genetics ; Interleukin-1beta - metabolism ; matrix metalloproteinase (MMPs) ; Matrix Metalloproteinase 2 - genetics ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase 9 - genetics ; Matrix Metalloproteinase 9 - metabolism ; Stromal Cells - enzymology ; Tumor Necrosis Factor-alpha - genetics ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>Journal of Veterinary Medical Science, 2012, Vol.74(5), pp.633-636</ispartof><rights>2012 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-ee0076b63e1cad8fa508b4f77c8ca390414925d8e4536f653f3e5559f494d25a3</citedby><cites>FETCH-LOGICAL-c541t-ee0076b63e1cad8fa508b4f77c8ca390414925d8e4536f653f3e5559f494d25a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22167104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HARAGUCHI, Tomoya</creatorcontrib><creatorcontrib>TANI, Kenji</creatorcontrib><creatorcontrib>KOGA, Masato</creatorcontrib><creatorcontrib>ODA, Yasutaka</creatorcontrib><creatorcontrib>ITAMOTO, Kazuhito</creatorcontrib><creatorcontrib>YAMAMOTO, Naoki</creatorcontrib><creatorcontrib>TERAI, Shuji</creatorcontrib><creatorcontrib>SAKAIDA, Isao</creatorcontrib><creatorcontrib>NAKAZAWA, Hiroshi</creatorcontrib><creatorcontrib>TAURA, Yasuho</creatorcontrib><title>Matrix Metalloproteinases (MMPs) Activity in Cultured Canine Bone Marrow Stromal Cells (BMSCs)</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Autologous bone marrow stromal cells (BMSCs) infusion therapy improves the hepatic fibrosis. To investigate the mechanism of remission, we evaluated the matrix metalloproteinase (MMP)-2 and -9 activity in canine BMSCs and the effect of pro-inflammatory cytokines on their expression. The activity and the gene expression of MMPs were analyzed by gelatin zymography and quantitative RT-PCR, respectively. The specific gelatinase bands were indicative effect of MMP-2 and -9 in canine BMSCs. MMP-2 expression seemed to be increased by TNF-α and IL-1β while MMP-9 was enhanced by TNF-α and IL-6. These results suggested that remissive effect on liver fibrosis might be partly attributable to the MMP-2 and -9 activity in BMSCs under the inflammatory condition.</description><subject>Animals</subject><subject>Bone Marrow Cells - enzymology</subject><subject>bone marrow stromal cell (BMSC)</subject><subject>canine</subject><subject>Cells, Cultured</subject><subject>culture</subject><subject>Dogs - metabolism</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>inflammatory cytokines</subject><subject>Interleukin-1beta - genetics</subject><subject>Interleukin-1beta - metabolism</subject><subject>matrix metalloproteinase (MMPs)</subject><subject>Matrix Metalloproteinase 2 - genetics</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Matrix Metalloproteinase 9 - genetics</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>Stromal Cells - enzymology</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkMtO3DAUQC3UCqa0O9aVpW5AaqifcbxkokIrEYFEu63lcW6oR3lQ24Hy93g0wyzYXC98fHR9EDqh5Jwyzb6tH4d4TmlBuJYHaEG5UIUSXL9DC6JpWSgmyRH6EOOaEEZFqQ_REWO0VJSIBfrT2BT8f9xAsn0_PYQpgR9thIhPm-Y2nuELl_yjT8_Yj7ie-zQHaHFtRz8CXk55NDaE6QnfpTANtsc19H1-vGzu6nj2Eb3vbB_h0-48Rr8vv_-qfxTXN1c_64vrwklBUwFAiCpXJQfqbFt1VpJqJTqlXOUs10RQoZlsKxCSl10pecdBSqk7oUXLpOXH6HTrzR_4N0NMZvDR5U3sCNMcDSWMVYIyUmb0yxt0Pc1hzNuZXKdSjAleZerrlnJhijFAZx6CH2x4ziqz6W423Q2lZtM945930nk1QLuHX0NnYLkF1jHZe9gDNiTvetjalDByM3bW_aX7a4OBkb8AcjyUJQ</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>HARAGUCHI, Tomoya</creator><creator>TANI, Kenji</creator><creator>KOGA, Masato</creator><creator>ODA, Yasutaka</creator><creator>ITAMOTO, Kazuhito</creator><creator>YAMAMOTO, Naoki</creator><creator>TERAI, Shuji</creator><creator>SAKAIDA, Isao</creator><creator>NAKAZAWA, Hiroshi</creator><creator>TAURA, Yasuho</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20120501</creationdate><title>Matrix Metalloproteinases (MMPs) Activity in Cultured Canine Bone Marrow Stromal Cells (BMSCs)</title><author>HARAGUCHI, Tomoya ; TANI, Kenji ; KOGA, Masato ; ODA, Yasutaka ; ITAMOTO, Kazuhito ; YAMAMOTO, Naoki ; TERAI, Shuji ; SAKAIDA, Isao ; NAKAZAWA, Hiroshi ; TAURA, Yasuho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-ee0076b63e1cad8fa508b4f77c8ca390414925d8e4536f653f3e5559f494d25a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Bone Marrow Cells - enzymology</topic><topic>bone marrow stromal cell (BMSC)</topic><topic>canine</topic><topic>Cells, Cultured</topic><topic>culture</topic><topic>Dogs - metabolism</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>inflammatory cytokines</topic><topic>Interleukin-1beta - genetics</topic><topic>Interleukin-1beta - metabolism</topic><topic>matrix metalloproteinase (MMPs)</topic><topic>Matrix Metalloproteinase 2 - genetics</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Matrix Metalloproteinase 9 - genetics</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>Stromal Cells - enzymology</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HARAGUCHI, Tomoya</creatorcontrib><creatorcontrib>TANI, Kenji</creatorcontrib><creatorcontrib>KOGA, Masato</creatorcontrib><creatorcontrib>ODA, Yasutaka</creatorcontrib><creatorcontrib>ITAMOTO, Kazuhito</creatorcontrib><creatorcontrib>YAMAMOTO, Naoki</creatorcontrib><creatorcontrib>TERAI, Shuji</creatorcontrib><creatorcontrib>SAKAIDA, Isao</creatorcontrib><creatorcontrib>NAKAZAWA, Hiroshi</creatorcontrib><creatorcontrib>TAURA, Yasuho</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HARAGUCHI, Tomoya</au><au>TANI, Kenji</au><au>KOGA, Masato</au><au>ODA, Yasutaka</au><au>ITAMOTO, Kazuhito</au><au>YAMAMOTO, Naoki</au><au>TERAI, Shuji</au><au>SAKAIDA, Isao</au><au>NAKAZAWA, Hiroshi</au><au>TAURA, Yasuho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Matrix Metalloproteinases (MMPs) Activity in Cultured Canine Bone Marrow Stromal Cells (BMSCs)</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>74</volume><issue>5</issue><spage>633</spage><epage>636</epage><pages>633-636</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Autologous bone marrow stromal cells (BMSCs) infusion therapy improves the hepatic fibrosis. To investigate the mechanism of remission, we evaluated the matrix metalloproteinase (MMP)-2 and -9 activity in canine BMSCs and the effect of pro-inflammatory cytokines on their expression. The activity and the gene expression of MMPs were analyzed by gelatin zymography and quantitative RT-PCR, respectively. The specific gelatinase bands were indicative effect of MMP-2 and -9 in canine BMSCs. MMP-2 expression seemed to be increased by TNF-α and IL-1β while MMP-9 was enhanced by TNF-α and IL-6. 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| subjects | Animals Bone Marrow Cells - enzymology bone marrow stromal cell (BMSC) canine Cells, Cultured culture Dogs - metabolism Gene Expression Regulation, Enzymologic - physiology inflammatory cytokines Interleukin-1beta - genetics Interleukin-1beta - metabolism matrix metalloproteinase (MMPs) Matrix Metalloproteinase 2 - genetics Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 9 - genetics Matrix Metalloproteinase 9 - metabolism Stromal Cells - enzymology Tumor Necrosis Factor-alpha - genetics Tumor Necrosis Factor-alpha - metabolism |
| title | Matrix Metalloproteinases (MMPs) Activity in Cultured Canine Bone Marrow Stromal Cells (BMSCs) |
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