Chmp1A acts as a tumor suppressor gene that inhibits proliferation of renal cell carcinoma
Abstract Renal cell carcinoma (RCC) is a highly malignant and often fatal disease of the kidney. Chmp1A is a member of the Endosomal Sorting Complex Required for Transport (ESCRT-III) family, and plays a role in the cytoplasm in sorting proteins to the multivesicular body (MVB). Chmp1A functions as...
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| Veröffentlicht in: | Cancer letters 2012-06, Vol.319 (2), p.190-196 |
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| creator | You, Zhenqiang Xin, Yanfei Liu, Yan Sun, Junying Zhou, Guoliang Gao, Haiyan Xu, Pansheng Chen, Yunxiang Chen, Guochan Zhang, Lijiang Gu, Liqiang Chen, Zhiqin Han, Bin Xuan, Yaoxian |
| description | Abstract Renal cell carcinoma (RCC) is a highly malignant and often fatal disease of the kidney. Chmp1A is a member of the Endosomal Sorting Complex Required for Transport (ESCRT-III) family, and plays a role in the cytoplasm in sorting proteins to the multivesicular body (MVB). Chmp1A functions as a tumor suppressor gene and has been reported in pancreatic tumor cells. Here, we examined the expression level of Chmp1A in human RCC tissues and renal tumor cells by real-time quantitative RT-PCR and western blot. We found that the expression level of Chmp1A is significantly lower in RCC tissues and renal tumor cells compared with adjacent non-tumorous tissues and normal renal cells. Additionally, inhibition of Chmp1A expression by shRNA induced tumor formation in normal renal cells. However, inhibition of Chmp1A did not significantly affect tumor cell proliferation in vitro and tumor progression in vivo . Interestingly, overexpression of Chmp1A using a eukaryotic plasmid inhibited the proliferation of renal tumor cells in vitro and the growth of renal tumor in vivo . Thus, our results demonstrate that Chmp1A functions as a tumor suppressor gene in renal cells and may be a useful target for treatment of RCC. |
| doi_str_mv | 10.1016/j.canlet.2012.01.010 |
| format | Article |
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Chmp1A is a member of the Endosomal Sorting Complex Required for Transport (ESCRT-III) family, and plays a role in the cytoplasm in sorting proteins to the multivesicular body (MVB). Chmp1A functions as a tumor suppressor gene and has been reported in pancreatic tumor cells. Here, we examined the expression level of Chmp1A in human RCC tissues and renal tumor cells by real-time quantitative RT-PCR and western blot. We found that the expression level of Chmp1A is significantly lower in RCC tissues and renal tumor cells compared with adjacent non-tumorous tissues and normal renal cells. Additionally, inhibition of Chmp1A expression by shRNA induced tumor formation in normal renal cells. However, inhibition of Chmp1A did not significantly affect tumor cell proliferation in vitro and tumor progression in vivo . Interestingly, overexpression of Chmp1A using a eukaryotic plasmid inhibited the proliferation of renal tumor cells in vitro and the growth of renal tumor in vivo . Thus, our results demonstrate that Chmp1A functions as a tumor suppressor gene in renal cells and may be a useful target for treatment of RCC.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2012.01.010</identifier><identifier>PMID: 22261332</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>carcinoma ; Carcinoma, Renal Cell - genetics ; Cell growth ; Cell Line, Tumor ; Cell Proliferation ; Cloning ; Cytoplasm ; Down-Regulation ; Endosomal Sorting Complexes Required for Transport - genetics ; Endosomal Sorting Complexes Required for Transport - metabolism ; Gene expression ; Gene Knockdown Techniques ; gene overexpression ; Genes, Suppressor ; Grants ; Hematology, Oncology and Palliative Medicine ; Humans ; Kidney ; kidney cells ; Kidney diseases ; Kidney Neoplasms - genetics ; Pancreas ; pancreatic neoplasms ; Plasmids ; Polymerase chain reaction ; Proteins ; renal cell carcinoma ; RNA, Small Interfering - pharmacology ; Studies ; Transfection ; Tumor cells ; Tumor suppressor genes ; Tumors ; Western blotting</subject><ispartof>Cancer letters, 2012-06, Vol.319 (2), p.190-196</ispartof><rights>Elsevier Ireland Ltd</rights><rights>Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Jun 28, 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-c5ed919e484e5289266c4dd65ea8bd3fb367706c62347b5883fcfa3f6bc33d7b3</citedby><cites>FETCH-LOGICAL-c447t-c5ed919e484e5289266c4dd65ea8bd3fb367706c62347b5883fcfa3f6bc33d7b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22261332$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>You, Zhenqiang</creatorcontrib><creatorcontrib>Xin, Yanfei</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Sun, Junying</creatorcontrib><creatorcontrib>Zhou, Guoliang</creatorcontrib><creatorcontrib>Gao, Haiyan</creatorcontrib><creatorcontrib>Xu, Pansheng</creatorcontrib><creatorcontrib>Chen, Yunxiang</creatorcontrib><creatorcontrib>Chen, Guochan</creatorcontrib><creatorcontrib>Zhang, Lijiang</creatorcontrib><creatorcontrib>Gu, Liqiang</creatorcontrib><creatorcontrib>Chen, Zhiqin</creatorcontrib><creatorcontrib>Han, Bin</creatorcontrib><creatorcontrib>Xuan, Yaoxian</creatorcontrib><title>Chmp1A acts as a tumor suppressor gene that inhibits proliferation of renal cell carcinoma</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Abstract Renal cell carcinoma (RCC) is a highly malignant and often fatal disease of the kidney. Chmp1A is a member of the Endosomal Sorting Complex Required for Transport (ESCRT-III) family, and plays a role in the cytoplasm in sorting proteins to the multivesicular body (MVB). Chmp1A functions as a tumor suppressor gene and has been reported in pancreatic tumor cells. Here, we examined the expression level of Chmp1A in human RCC tissues and renal tumor cells by real-time quantitative RT-PCR and western blot. We found that the expression level of Chmp1A is significantly lower in RCC tissues and renal tumor cells compared with adjacent non-tumorous tissues and normal renal cells. Additionally, inhibition of Chmp1A expression by shRNA induced tumor formation in normal renal cells. However, inhibition of Chmp1A did not significantly affect tumor cell proliferation in vitro and tumor progression in vivo . Interestingly, overexpression of Chmp1A using a eukaryotic plasmid inhibited the proliferation of renal tumor cells in vitro and the growth of renal tumor in vivo . Thus, our results demonstrate that Chmp1A functions as a tumor suppressor gene in renal cells and may be a useful target for treatment of RCC.</description><subject>carcinoma</subject><subject>Carcinoma, Renal Cell - genetics</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Cloning</subject><subject>Cytoplasm</subject><subject>Down-Regulation</subject><subject>Endosomal Sorting Complexes Required for Transport - genetics</subject><subject>Endosomal Sorting Complexes Required for Transport - metabolism</subject><subject>Gene expression</subject><subject>Gene Knockdown Techniques</subject><subject>gene overexpression</subject><subject>Genes, Suppressor</subject><subject>Grants</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Humans</subject><subject>Kidney</subject><subject>kidney cells</subject><subject>Kidney diseases</subject><subject>Kidney Neoplasms - genetics</subject><subject>Pancreas</subject><subject>pancreatic neoplasms</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>renal cell carcinoma</subject><subject>RNA, Small Interfering - pharmacology</subject><subject>Studies</subject><subject>Transfection</subject><subject>Tumor cells</subject><subject>Tumor suppressor genes</subject><subject>Tumors</subject><subject>Western blotting</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhi0EotvCP0AQiQuXLOPv7AWpWkFBqsSh9MLFcpxJ10s-Fjup1H_PRCkgcUIa2T488_qdD8Zecdhy4Ob9cRv80OG0FcDFFjgFPGEbXllR2l0FT9kGJKhSVlKfsfOcjwCgldXP2ZkQwnApxYZ93x_6E78sfJhy4SmKae7HVOT5dEqYMz3vcMBiOvipiMMh1pHAUxq72GLyUxyHYmyLhIPvioAdHT6FOIy9f8Getb7L-PLxvmC3nz5-238ur79efdlfXpdBKTuVQWOz4ztUlUItqp0wJqimMRp9VTeyraWxFkwwQipb66qSbWi9bE0dpGxsLS_Yu1WXXP2cMU-uj3mx4gcc5-w4CKGN0JL_BwqgqGuiIvTtP-hxnBNVSZQGA9YIbohSKxXSmHPC1p1S7H16ICm3jMkd3Tomt4zJAacASnv9KD7XPTZ_kn7PhYA3K9D60fm7FLO7vSEFTQYl-VtK-bASSK29j5hc6OIQg-9-4APmv2Zdpm_dzbILyypwsUhYK38B4kGscw</recordid><startdate>20120628</startdate><enddate>20120628</enddate><creator>You, Zhenqiang</creator><creator>Xin, Yanfei</creator><creator>Liu, Yan</creator><creator>Sun, Junying</creator><creator>Zhou, Guoliang</creator><creator>Gao, Haiyan</creator><creator>Xu, Pansheng</creator><creator>Chen, Yunxiang</creator><creator>Chen, Guochan</creator><creator>Zhang, Lijiang</creator><creator>Gu, Liqiang</creator><creator>Chen, Zhiqin</creator><creator>Han, Bin</creator><creator>Xuan, Yaoxian</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20120628</creationdate><title>Chmp1A acts as a tumor suppressor gene that inhibits proliferation of renal cell carcinoma</title><author>You, Zhenqiang ; Xin, Yanfei ; Liu, Yan ; Sun, Junying ; Zhou, Guoliang ; Gao, Haiyan ; Xu, Pansheng ; Chen, Yunxiang ; Chen, Guochan ; Zhang, Lijiang ; Gu, Liqiang ; Chen, Zhiqin ; Han, Bin ; Xuan, Yaoxian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-c5ed919e484e5289266c4dd65ea8bd3fb367706c62347b5883fcfa3f6bc33d7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>carcinoma</topic><topic>Carcinoma, Renal Cell - genetics</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Cloning</topic><topic>Cytoplasm</topic><topic>Down-Regulation</topic><topic>Endosomal Sorting Complexes Required for Transport - genetics</topic><topic>Endosomal Sorting Complexes Required for Transport - metabolism</topic><topic>Gene expression</topic><topic>Gene Knockdown Techniques</topic><topic>gene overexpression</topic><topic>Genes, Suppressor</topic><topic>Grants</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Humans</topic><topic>Kidney</topic><topic>kidney cells</topic><topic>Kidney diseases</topic><topic>Kidney Neoplasms - genetics</topic><topic>Pancreas</topic><topic>pancreatic neoplasms</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>renal cell carcinoma</topic><topic>RNA, Small Interfering - pharmacology</topic><topic>Studies</topic><topic>Transfection</topic><topic>Tumor cells</topic><topic>Tumor suppressor genes</topic><topic>Tumors</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>You, Zhenqiang</creatorcontrib><creatorcontrib>Xin, Yanfei</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Sun, Junying</creatorcontrib><creatorcontrib>Zhou, Guoliang</creatorcontrib><creatorcontrib>Gao, Haiyan</creatorcontrib><creatorcontrib>Xu, Pansheng</creatorcontrib><creatorcontrib>Chen, Yunxiang</creatorcontrib><creatorcontrib>Chen, Guochan</creatorcontrib><creatorcontrib>Zhang, Lijiang</creatorcontrib><creatorcontrib>Gu, Liqiang</creatorcontrib><creatorcontrib>Chen, Zhiqin</creatorcontrib><creatorcontrib>Han, Bin</creatorcontrib><creatorcontrib>Xuan, Yaoxian</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>You, Zhenqiang</au><au>Xin, Yanfei</au><au>Liu, Yan</au><au>Sun, Junying</au><au>Zhou, Guoliang</au><au>Gao, Haiyan</au><au>Xu, Pansheng</au><au>Chen, Yunxiang</au><au>Chen, Guochan</au><au>Zhang, Lijiang</au><au>Gu, Liqiang</au><au>Chen, Zhiqin</au><au>Han, Bin</au><au>Xuan, Yaoxian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chmp1A acts as a tumor suppressor gene that inhibits proliferation of renal cell carcinoma</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2012-06-28</date><risdate>2012</risdate><volume>319</volume><issue>2</issue><spage>190</spage><epage>196</epage><pages>190-196</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>Abstract Renal cell carcinoma (RCC) is a highly malignant and often fatal disease of the kidney. Chmp1A is a member of the Endosomal Sorting Complex Required for Transport (ESCRT-III) family, and plays a role in the cytoplasm in sorting proteins to the multivesicular body (MVB). Chmp1A functions as a tumor suppressor gene and has been reported in pancreatic tumor cells. Here, we examined the expression level of Chmp1A in human RCC tissues and renal tumor cells by real-time quantitative RT-PCR and western blot. We found that the expression level of Chmp1A is significantly lower in RCC tissues and renal tumor cells compared with adjacent non-tumorous tissues and normal renal cells. Additionally, inhibition of Chmp1A expression by shRNA induced tumor formation in normal renal cells. However, inhibition of Chmp1A did not significantly affect tumor cell proliferation in vitro and tumor progression in vivo . Interestingly, overexpression of Chmp1A using a eukaryotic plasmid inhibited the proliferation of renal tumor cells in vitro and the growth of renal tumor in vivo . Thus, our results demonstrate that Chmp1A functions as a tumor suppressor gene in renal cells and may be a useful target for treatment of RCC.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>22261332</pmid><doi>10.1016/j.canlet.2012.01.010</doi><tpages>7</tpages></addata></record> |
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| subjects | carcinoma Carcinoma, Renal Cell - genetics Cell growth Cell Line, Tumor Cell Proliferation Cloning Cytoplasm Down-Regulation Endosomal Sorting Complexes Required for Transport - genetics Endosomal Sorting Complexes Required for Transport - metabolism Gene expression Gene Knockdown Techniques gene overexpression Genes, Suppressor Grants Hematology, Oncology and Palliative Medicine Humans Kidney kidney cells Kidney diseases Kidney Neoplasms - genetics Pancreas pancreatic neoplasms Plasmids Polymerase chain reaction Proteins renal cell carcinoma RNA, Small Interfering - pharmacology Studies Transfection Tumor cells Tumor suppressor genes Tumors Western blotting |
| title | Chmp1A acts as a tumor suppressor gene that inhibits proliferation of renal cell carcinoma |
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