Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis
Studies have shown that male germline stem cells (mGSCs), which are responsible for maintaining spermatogenesis in the male, could be obtained from mouse and human testis. However, the traditional cultural methods were mostly dependent on serum and feeder, and the initial mGSCs were either obtained...
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| Veröffentlicht in: | Journal of cellular biochemistry 2011-04, Vol.112 (4), p.1009-1021 |
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| creator | Zhang, Shanshan Sun, Junwei Pan, Shaohui Zhu, Haijing Wang, Long Hu, Yue Wang, Jing Wang, Fang Cao, Hui Yan, Xinrong Hua, Jinlian |
| description | Studies have shown that male germline stem cells (mGSCs), which are responsible for maintaining spermatogenesis in the male, could be obtained from mouse and human testis. However, the traditional cultural methods were mostly dependent on serum and feeder, and the initial mGSCs were either obtained from neonatal mice or the detailed description of its potency and origin was not provided. Here we reported a novel (retinol (RE) serum‐free and feeder‐free) system for the successful culture of adult germline stem cells from adult Kunming mice (8–24 weeks) testis. The isolated mGSCs cultured in RE serum‐free and feeder‐free medium maintained the typical morphology of undifferentiated embryonic stem cells (ESCs), and they proliferated well in RE medium analyzed by proliferation assay, RT‐PCR, microarray, and Western blotting. These cells also showed typical properties of ESCs (alkaline phosphatase (AP) positive, expressions of Oct4, Sox2, Nanog, and SSEA1, with the capacity to form teratomas and differentiate into various types of cells within three germ layers). Taken together, we conclude that RE promotes the self‐renewal of mGSCs and maintains the pluripotency of mGSCs, the RE serum‐free and feeder‐free system may be useful for the culture of pluripotent stem cell lines from adult testis tissues, which provides a new resource for tissue engineering and therapy for infertility. J. Cell. Biochem. 112: 1009–1021, 2011. © 2011 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jcb.23029 |
| format | Article |
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However, the traditional cultural methods were mostly dependent on serum and feeder, and the initial mGSCs were either obtained from neonatal mice or the detailed description of its potency and origin was not provided. Here we reported a novel (retinol (RE) serum‐free and feeder‐free) system for the successful culture of adult germline stem cells from adult Kunming mice (8–24 weeks) testis. The isolated mGSCs cultured in RE serum‐free and feeder‐free medium maintained the typical morphology of undifferentiated embryonic stem cells (ESCs), and they proliferated well in RE medium analyzed by proliferation assay, RT‐PCR, microarray, and Western blotting. These cells also showed typical properties of ESCs (alkaline phosphatase (AP) positive, expressions of Oct4, Sox2, Nanog, and SSEA1, with the capacity to form teratomas and differentiate into various types of cells within three germ layers). Taken together, we conclude that RE promotes the self‐renewal of mGSCs and maintains the pluripotency of mGSCs, the RE serum‐free and feeder‐free system may be useful for the culture of pluripotent stem cell lines from adult testis tissues, which provides a new resource for tissue engineering and therapy for infertility. J. Cell. Biochem. 112: 1009–1021, 2011. © 2011 Wiley‐Liss, Inc.</description><identifier>ISSN: 0730-2312</identifier><identifier>ISSN: 1097-4644</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.23029</identifier><identifier>PMID: 21308744</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Alkaline phosphatase ; Alkaline Phosphatase - metabolism ; Animals ; Blotting, Western ; Cell culture ; Cell Culture Techniques ; Cell Differentiation - drug effects ; Cell proliferation ; Cell Proliferation - drug effects ; Cells, Cultured ; Culture Media, Serum-Free - pharmacology ; Embryo cells ; embryonic stem cell (ESC) ; germ cells ; Glial Cell Line-Derived Neurotrophic Factor - pharmacology ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; Infertility ; Lewis X Antigen - genetics ; Lewis X Antigen - metabolism ; Male ; male germline stem cells (mGSCs) ; Mice ; mouse ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - drug effects ; Myocytes, Cardiac - metabolism ; Nanog Homeobox Protein ; Neonates ; Neurons - cytology ; Neurons - drug effects ; Neurons - metabolism ; Oct-4 protein ; Octamer Transcription Factor-3 - genetics ; Octamer Transcription Factor-3 - metabolism ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - drug effects ; Pluripotent Stem Cells - metabolism ; Polymerase chain reaction ; retinol (RE) ; Reverse Transcriptase Polymerase Chain Reaction ; serum-free and feeder-free ; SOXB1 Transcription Factors - genetics ; SOXB1 Transcription Factors - metabolism ; Spermatogenesis ; Spermatozoa - cytology ; Spermatozoa - drug effects ; Spermatozoa - metabolism ; Stem cells ; teratoma ; Teratoma - genetics ; Teratoma - metabolism ; Teratoma - pathology ; Testes ; Testis - cytology ; Tissue engineering ; Vitamin A ; Vitamin A - pharmacology ; Vitamins - pharmacology ; Western blotting</subject><ispartof>Journal of cellular biochemistry, 2011-04, Vol.112 (4), p.1009-1021</ispartof><rights>Copyright © 2011 Wiley‐Liss, Inc.</rights><rights>Copyright © 2011 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4619-ae1c13d2f14e7a63b48543e955ed214324337f036a8252f78ce8b50055b97f6a3</citedby><cites>FETCH-LOGICAL-c4619-ae1c13d2f14e7a63b48543e955ed214324337f036a8252f78ce8b50055b97f6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.23029$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.23029$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27926,27927,45576,45577</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21308744$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Shanshan</creatorcontrib><creatorcontrib>Sun, Junwei</creatorcontrib><creatorcontrib>Pan, Shaohui</creatorcontrib><creatorcontrib>Zhu, Haijing</creatorcontrib><creatorcontrib>Wang, Long</creatorcontrib><creatorcontrib>Hu, Yue</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>Cao, Hui</creatorcontrib><creatorcontrib>Yan, Xinrong</creatorcontrib><creatorcontrib>Hua, Jinlian</creatorcontrib><title>Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis</title><title>Journal of cellular biochemistry</title><addtitle>J. Cell. Biochem</addtitle><description>Studies have shown that male germline stem cells (mGSCs), which are responsible for maintaining spermatogenesis in the male, could be obtained from mouse and human testis. However, the traditional cultural methods were mostly dependent on serum and feeder, and the initial mGSCs were either obtained from neonatal mice or the detailed description of its potency and origin was not provided. Here we reported a novel (retinol (RE) serum‐free and feeder‐free) system for the successful culture of adult germline stem cells from adult Kunming mice (8–24 weeks) testis. The isolated mGSCs cultured in RE serum‐free and feeder‐free medium maintained the typical morphology of undifferentiated embryonic stem cells (ESCs), and they proliferated well in RE medium analyzed by proliferation assay, RT‐PCR, microarray, and Western blotting. These cells also showed typical properties of ESCs (alkaline phosphatase (AP) positive, expressions of Oct4, Sox2, Nanog, and SSEA1, with the capacity to form teratomas and differentiate into various types of cells within three germ layers). Taken together, we conclude that RE promotes the self‐renewal of mGSCs and maintains the pluripotency of mGSCs, the RE serum‐free and feeder‐free system may be useful for the culture of pluripotent stem cell lines from adult testis tissues, which provides a new resource for tissue engineering and therapy for infertility. J. Cell. Biochem. 112: 1009–1021, 2011. © 2011 Wiley‐Liss, Inc.</description><subject>Alkaline phosphatase</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Culture Media, Serum-Free - pharmacology</subject><subject>Embryo cells</subject><subject>embryonic stem cell (ESC)</subject><subject>germ cells</subject><subject>Glial Cell Line-Derived Neurotrophic Factor - pharmacology</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Infertility</subject><subject>Lewis X Antigen - genetics</subject><subject>Lewis X Antigen - metabolism</subject><subject>Male</subject><subject>male germline stem cells (mGSCs)</subject><subject>Mice</subject><subject>mouse</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - drug effects</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>Nanog Homeobox Protein</subject><subject>Neonates</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Oct-4 protein</subject><subject>Octamer Transcription Factor-3 - genetics</subject><subject>Octamer Transcription Factor-3 - metabolism</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pluripotent Stem Cells - drug effects</subject><subject>Pluripotent Stem Cells - metabolism</subject><subject>Polymerase chain reaction</subject><subject>retinol (RE)</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>serum-free and feeder-free</subject><subject>SOXB1 Transcription Factors - genetics</subject><subject>SOXB1 Transcription Factors - metabolism</subject><subject>Spermatogenesis</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - metabolism</subject><subject>Stem cells</subject><subject>teratoma</subject><subject>Teratoma - genetics</subject><subject>Teratoma - metabolism</subject><subject>Teratoma - pathology</subject><subject>Testes</subject><subject>Testis - cytology</subject><subject>Tissue engineering</subject><subject>Vitamin A</subject><subject>Vitamin A - pharmacology</subject><subject>Vitamins - pharmacology</subject><subject>Western blotting</subject><issn>0730-2312</issn><issn>1097-4644</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMFu1DAQhi0EokvhwAsgH3cPaW2PHSfHsoKFUkG1gDhaTnaMXOxkayeUvj0u2_bGYTSX7_9n9BHymrMTzpg4veq7EwFMtE_IgrNWV7KW8ilZMA2sEsDFEXmR8xVjrG1BPCdHggNrtJQLkrY4-WEMdPnbTzb6gZ6taLR-mMpkmjG4KuGANzbQ0dF9mJPfjxMOU6EC0p-YYvAD0jxhpD2GkOkybr6u84q6NEZqd3Mo7DhnpBPmyeeX5JmzIeOr-31Mvr9_9239obr4svm4PruoelnztrLIew474bhEbWvoZKMkYKsU7gSXICSAdgxq2wglnG56bDrFmFJdq11t4ZgsD737NF7P5bSJPt99aAcs7xjOhFA1a4AXdHVA-zTmnNCZffLRptsCmTvFpig2_xQX9s197dxF3D2SD04LcHoAbnzA2_83mfP124fK6pDwxeKfx4RNv0ytQSvz4_PGfDqH7eXltjUb-Av9QJPU</recordid><startdate>201104</startdate><enddate>201104</enddate><creator>Zhang, Shanshan</creator><creator>Sun, Junwei</creator><creator>Pan, Shaohui</creator><creator>Zhu, Haijing</creator><creator>Wang, Long</creator><creator>Hu, Yue</creator><creator>Wang, Jing</creator><creator>Wang, Fang</creator><creator>Cao, Hui</creator><creator>Yan, Xinrong</creator><creator>Hua, Jinlian</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201104</creationdate><title>Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis</title><author>Zhang, Shanshan ; Sun, Junwei ; Pan, Shaohui ; Zhu, Haijing ; Wang, Long ; Hu, Yue ; Wang, Jing ; Wang, Fang ; Cao, Hui ; Yan, Xinrong ; Hua, Jinlian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4619-ae1c13d2f14e7a63b48543e955ed214324337f036a8252f78ce8b50055b97f6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Alkaline phosphatase</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cell culture</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Culture Media, Serum-Free - pharmacology</topic><topic>Embryo cells</topic><topic>embryonic stem cell (ESC)</topic><topic>germ cells</topic><topic>Glial Cell Line-Derived Neurotrophic Factor - pharmacology</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Infertility</topic><topic>Lewis X Antigen - genetics</topic><topic>Lewis X Antigen - metabolism</topic><topic>Male</topic><topic>male germline stem cells (mGSCs)</topic><topic>Mice</topic><topic>mouse</topic><topic>Myocytes, Cardiac - cytology</topic><topic>Myocytes, Cardiac - drug effects</topic><topic>Myocytes, Cardiac - metabolism</topic><topic>Nanog Homeobox Protein</topic><topic>Neonates</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Oct-4 protein</topic><topic>Octamer Transcription Factor-3 - genetics</topic><topic>Octamer Transcription Factor-3 - metabolism</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Pluripotent Stem Cells - drug effects</topic><topic>Pluripotent Stem Cells - metabolism</topic><topic>Polymerase chain reaction</topic><topic>retinol (RE)</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>serum-free and feeder-free</topic><topic>SOXB1 Transcription Factors - genetics</topic><topic>SOXB1 Transcription Factors - metabolism</topic><topic>Spermatogenesis</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - metabolism</topic><topic>Stem cells</topic><topic>teratoma</topic><topic>Teratoma - genetics</topic><topic>Teratoma - metabolism</topic><topic>Teratoma - pathology</topic><topic>Testes</topic><topic>Testis - cytology</topic><topic>Tissue engineering</topic><topic>Vitamin A</topic><topic>Vitamin A - pharmacology</topic><topic>Vitamins - pharmacology</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Shanshan</creatorcontrib><creatorcontrib>Sun, Junwei</creatorcontrib><creatorcontrib>Pan, Shaohui</creatorcontrib><creatorcontrib>Zhu, Haijing</creatorcontrib><creatorcontrib>Wang, Long</creatorcontrib><creatorcontrib>Hu, Yue</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Wang, Fang</creatorcontrib><creatorcontrib>Cao, Hui</creatorcontrib><creatorcontrib>Yan, Xinrong</creatorcontrib><creatorcontrib>Hua, Jinlian</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Shanshan</au><au>Sun, Junwei</au><au>Pan, Shaohui</au><au>Zhu, Haijing</au><au>Wang, Long</au><au>Hu, Yue</au><au>Wang, Jing</au><au>Wang, Fang</au><au>Cao, Hui</au><au>Yan, Xinrong</au><au>Hua, Jinlian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J. Cell. Biochem</addtitle><date>2011-04</date><risdate>2011</risdate><volume>112</volume><issue>4</issue><spage>1009</spage><epage>1021</epage><pages>1009-1021</pages><issn>0730-2312</issn><issn>1097-4644</issn><eissn>1097-4644</eissn><abstract>Studies have shown that male germline stem cells (mGSCs), which are responsible for maintaining spermatogenesis in the male, could be obtained from mouse and human testis. However, the traditional cultural methods were mostly dependent on serum and feeder, and the initial mGSCs were either obtained from neonatal mice or the detailed description of its potency and origin was not provided. Here we reported a novel (retinol (RE) serum‐free and feeder‐free) system for the successful culture of adult germline stem cells from adult Kunming mice (8–24 weeks) testis. The isolated mGSCs cultured in RE serum‐free and feeder‐free medium maintained the typical morphology of undifferentiated embryonic stem cells (ESCs), and they proliferated well in RE medium analyzed by proliferation assay, RT‐PCR, microarray, and Western blotting. These cells also showed typical properties of ESCs (alkaline phosphatase (AP) positive, expressions of Oct4, Sox2, Nanog, and SSEA1, with the capacity to form teratomas and differentiate into various types of cells within three germ layers). Taken together, we conclude that RE promotes the self‐renewal of mGSCs and maintains the pluripotency of mGSCs, the RE serum‐free and feeder‐free system may be useful for the culture of pluripotent stem cell lines from adult testis tissues, which provides a new resource for tissue engineering and therapy for infertility. J. Cell. Biochem. 112: 1009–1021, 2011. © 2011 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>21308744</pmid><doi>10.1002/jcb.23029</doi><tpages>13</tpages></addata></record> |
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| ispartof | Journal of cellular biochemistry, 2011-04, Vol.112 (4), p.1009-1021 |
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| subjects | Alkaline phosphatase Alkaline Phosphatase - metabolism Animals Blotting, Western Cell culture Cell Culture Techniques Cell Differentiation - drug effects Cell proliferation Cell Proliferation - drug effects Cells, Cultured Culture Media, Serum-Free - pharmacology Embryo cells embryonic stem cell (ESC) germ cells Glial Cell Line-Derived Neurotrophic Factor - pharmacology Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Infertility Lewis X Antigen - genetics Lewis X Antigen - metabolism Male male germline stem cells (mGSCs) Mice mouse Myocytes, Cardiac - cytology Myocytes, Cardiac - drug effects Myocytes, Cardiac - metabolism Nanog Homeobox Protein Neonates Neurons - cytology Neurons - drug effects Neurons - metabolism Oct-4 protein Octamer Transcription Factor-3 - genetics Octamer Transcription Factor-3 - metabolism Pluripotent Stem Cells - cytology Pluripotent Stem Cells - drug effects Pluripotent Stem Cells - metabolism Polymerase chain reaction retinol (RE) Reverse Transcriptase Polymerase Chain Reaction serum-free and feeder-free SOXB1 Transcription Factors - genetics SOXB1 Transcription Factors - metabolism Spermatogenesis Spermatozoa - cytology Spermatozoa - drug effects Spermatozoa - metabolism Stem cells teratoma Teratoma - genetics Teratoma - metabolism Teratoma - pathology Testes Testis - cytology Tissue engineering Vitamin A Vitamin A - pharmacology Vitamins - pharmacology Western blotting |
| title | Retinol (vitamin A) maintains self-renewal of pluripotent male germline stem cells (mGSCs) from adult mouse testis |
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