In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model
For the treatment of ununited fractures, we developed a system of delivering magnetic labelled mesenchymal stromal cells (MSCs) using an extracorporeal magnetic device. In this study, we transplanted ferucarbotran-labelled and luciferase-positive bone marrow-derived MSCs into a non-healing femoral f...
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| Veröffentlicht in: | Journal of bone and joint surgery. British volume 2012-07, Vol.94 (7), p.998-1006 |
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| container_title | Journal of bone and joint surgery. British volume |
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| creator | KODAMA, A KAMEI, N KAMEI, G KONGCHAROENSOMBAT, W OHKAWA, S NAKABAYASHI, A OCHI, M |
| description | For the treatment of ununited fractures, we developed a system of delivering magnetic labelled mesenchymal stromal cells (MSCs) using an extracorporeal magnetic device. In this study, we transplanted ferucarbotran-labelled and luciferase-positive bone marrow-derived MSCs into a non-healing femoral fracture rat model in the presence of a magnetic field. The biological fate of the transplanted MSCs was observed using luciferase-based bioluminescence imaging and we found that the number of MSC derived photons increased from day one to day three and thereafter decreased over time. The magnetic cell delivery system induced the accumulation of photons at the fracture site, while also retaining higher photon intensity from day three to week four. Furthermore, radiological and histological findings suggested improved callus formation and endochondral ossification. We therefore believe that this delivery system may be a promising option for bone regeneration. |
| doi_str_mv | 10.1302/0301-620x.94b7.28521 |
| format | Article |
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In this study, we transplanted ferucarbotran-labelled and luciferase-positive bone marrow-derived MSCs into a non-healing femoral fracture rat model in the presence of a magnetic field. The biological fate of the transplanted MSCs was observed using luciferase-based bioluminescence imaging and we found that the number of MSC derived photons increased from day one to day three and thereafter decreased over time. The magnetic cell delivery system induced the accumulation of photons at the fracture site, while also retaining higher photon intensity from day three to week four. Furthermore, radiological and histological findings suggested improved callus formation and endochondral ossification. We therefore believe that this delivery system may be a promising option for bone regeneration.</description><identifier>ISSN: 0301-620X</identifier><identifier>EISSN: 2044-5377</identifier><identifier>DOI: 10.1302/0301-620x.94b7.28521</identifier><identifier>PMID: 22733960</identifier><identifier>CODEN: JBSUAK</identifier><language>eng</language><publisher>London: British Editorial Society of Bone and Joint Surgery</publisher><subject>Animals ; Biological and medical sciences ; Bone Regeneration - physiology ; Cell Differentiation - physiology ; Cell Movement ; Dextrans ; Disease Models, Animal ; Diseases of the osteoarticular system ; Female ; Femoral Fractures - pathology ; Femoral Fractures - physiopathology ; Femoral Fractures - therapy ; Fracture Healing - physiology ; Fractures, Ununited - pathology ; Fractures, Ununited - physiopathology ; Fractures, Ununited - therapy ; Injuries of the limb. Injuries of the spine ; Luminescent Measurements - methods ; Magnetic Fields ; Magnetite Nanoparticles ; Medical sciences ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells - pathology ; Mesenchymal Stromal Cells - physiology ; Orthopedic surgery ; Rats ; Rats, Inbred Lew ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Traumas. Diseases due to physical agents</subject><ispartof>Journal of bone and joint surgery. British volume, 2012-07, Vol.94 (7), p.998-1006</ispartof><rights>2015 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-3d3413628fb8e2ee92ce3d7c63da67188662a42d501a4650ddf8cc7dd58712233</citedby><cites>FETCH-LOGICAL-c403t-3d3413628fb8e2ee92ce3d7c63da67188662a42d501a4650ddf8cc7dd58712233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27928,27929</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26073882$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22733960$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KODAMA, A</creatorcontrib><creatorcontrib>KAMEI, N</creatorcontrib><creatorcontrib>KAMEI, G</creatorcontrib><creatorcontrib>KONGCHAROENSOMBAT, W</creatorcontrib><creatorcontrib>OHKAWA, S</creatorcontrib><creatorcontrib>NAKABAYASHI, A</creatorcontrib><creatorcontrib>OCHI, M</creatorcontrib><title>In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model</title><title>Journal of bone and joint surgery. British volume</title><addtitle>J Bone Joint Surg Br</addtitle><description>For the treatment of ununited fractures, we developed a system of delivering magnetic labelled mesenchymal stromal cells (MSCs) using an extracorporeal magnetic device. In this study, we transplanted ferucarbotran-labelled and luciferase-positive bone marrow-derived MSCs into a non-healing femoral fracture rat model in the presence of a magnetic field. The biological fate of the transplanted MSCs was observed using luciferase-based bioluminescence imaging and we found that the number of MSC derived photons increased from day one to day three and thereafter decreased over time. The magnetic cell delivery system induced the accumulation of photons at the fracture site, while also retaining higher photon intensity from day three to week four. Furthermore, radiological and histological findings suggested improved callus formation and endochondral ossification. We therefore believe that this delivery system may be a promising option for bone regeneration.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Regeneration - physiology</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Movement</subject><subject>Dextrans</subject><subject>Disease Models, Animal</subject><subject>Diseases of the osteoarticular system</subject><subject>Female</subject><subject>Femoral Fractures - pathology</subject><subject>Femoral Fractures - physiopathology</subject><subject>Femoral Fractures - therapy</subject><subject>Fracture Healing - physiology</subject><subject>Fractures, Ununited - pathology</subject><subject>Fractures, Ununited - physiopathology</subject><subject>Fractures, Ununited - therapy</subject><subject>Injuries of the limb. Injuries of the spine</subject><subject>Luminescent Measurements - methods</subject><subject>Magnetic Fields</subject><subject>Magnetite Nanoparticles</subject><subject>Medical sciences</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>Mesenchymal Stromal Cells - pathology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Orthopedic surgery</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Traumas. Diseases due to physical agents</subject><issn>0301-620X</issn><issn>2044-5377</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0c1u1DAQB3ALgehSeAOEfEHiksVfsb1HqIBWqsQFJG6RY08Wo8RePMnCvgTPjNNu4TSH-c3Y-g8hLznbcsnEWyYZb7Rgv7c71ZutsK3gj8hGMKWaVhrzmGweyLcL8gzxB2NMta18Si6EMFLuNNuQPzeJHuMx0z7mcZliAvSQPNA4uX1Me5oHOheX8DC6NEOgfU5AJ1dK_kUnwGq_nyY3UpxLXquHcUS64DrrKtwnmKOnAcZ4hHKieMIZJhpT7RY306E4Py-l7szVPCdPBjcivDjXS_L144cvV9fN7edPN1fvbhuvmJwbGaTiUgs79BYEwE54kMF4LYPThlurtXBKhJZxp3TLQhis9yaE1houhJSX5M393kPJPxfAuZsirl93CfKCHWdVmYptpeqe-pIRCwzdodRwyqmibr1Et8bcrTF3O_XedHeXqGOvzi8s_QTh39BD9BW8PgOH3o01h-Qj_neaGWmtkH8Bit6T1Q</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>KODAMA, A</creator><creator>KAMEI, N</creator><creator>KAMEI, G</creator><creator>KONGCHAROENSOMBAT, W</creator><creator>OHKAWA, S</creator><creator>NAKABAYASHI, A</creator><creator>OCHI, M</creator><general>British Editorial Society of Bone and Joint Surgery</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120701</creationdate><title>In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model</title><author>KODAMA, A ; KAMEI, N ; KAMEI, G ; KONGCHAROENSOMBAT, W ; OHKAWA, S ; NAKABAYASHI, A ; OCHI, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-3d3413628fb8e2ee92ce3d7c63da67188662a42d501a4650ddf8cc7dd58712233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Regeneration - physiology</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Movement</topic><topic>Dextrans</topic><topic>Disease Models, Animal</topic><topic>Diseases of the osteoarticular system</topic><topic>Female</topic><topic>Femoral Fractures - pathology</topic><topic>Femoral Fractures - physiopathology</topic><topic>Femoral Fractures - therapy</topic><topic>Fracture Healing - physiology</topic><topic>Fractures, Ununited - pathology</topic><topic>Fractures, Ununited - physiopathology</topic><topic>Fractures, Ununited - therapy</topic><topic>Injuries of the limb. Injuries of the spine</topic><topic>Luminescent Measurements - methods</topic><topic>Magnetic Fields</topic><topic>Magnetite Nanoparticles</topic><topic>Medical sciences</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>Mesenchymal Stromal Cells - pathology</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Orthopedic surgery</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Traumas. 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In this study, we transplanted ferucarbotran-labelled and luciferase-positive bone marrow-derived MSCs into a non-healing femoral fracture rat model in the presence of a magnetic field. The biological fate of the transplanted MSCs was observed using luciferase-based bioluminescence imaging and we found that the number of MSC derived photons increased from day one to day three and thereafter decreased over time. The magnetic cell delivery system induced the accumulation of photons at the fracture site, while also retaining higher photon intensity from day three to week four. Furthermore, radiological and histological findings suggested improved callus formation and endochondral ossification. We therefore believe that this delivery system may be a promising option for bone regeneration.</abstract><cop>London</cop><pub>British Editorial Society of Bone and Joint Surgery</pub><pmid>22733960</pmid><doi>10.1302/0301-620x.94b7.28521</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Bone Regeneration - physiology Cell Differentiation - physiology Cell Movement Dextrans Disease Models, Animal Diseases of the osteoarticular system Female Femoral Fractures - pathology Femoral Fractures - physiopathology Femoral Fractures - therapy Fracture Healing - physiology Fractures, Ununited - pathology Fractures, Ununited - physiopathology Fractures, Ununited - therapy Injuries of the limb. Injuries of the spine Luminescent Measurements - methods Magnetic Fields Magnetite Nanoparticles Medical sciences Mesenchymal Stem Cell Transplantation Mesenchymal Stromal Cells - pathology Mesenchymal Stromal Cells - physiology Orthopedic surgery Rats Rats, Inbred Lew Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Traumas. Diseases due to physical agents |
| title | In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model |
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