easyFRAP: an interactive, easy-to-use tool for qualitative and quantitative analysis of FRAP data
We present easyFRAP, a versatile tool that assists quantitative and qualitative analysis of fluorescence recovery after photobleaching (FRAP) data. The user can handle simultaneously large data sets of raw data, visualize fluorescence recovery curves, exclude low quality data, perform data normaliza...
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| Veröffentlicht in: | Bioinformatics (Oxford, England) England), 2012-07, Vol.28 (13), p.1800-1801 |
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| container_title | Bioinformatics (Oxford, England) |
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| creator | RAPSOMANIKI, Maria Anna KOTSANTIS, Panagiotis SYMEONIDOU, Loanna-Eleni GIAKOUMAKIS, Nickolaos-Nikiforos TARAVIRAS, Stavros LYGEROU, Zoi |
| description | We present easyFRAP, a versatile tool that assists quantitative and qualitative analysis of fluorescence recovery after photobleaching (FRAP) data. The user can handle simultaneously large data sets of raw data, visualize fluorescence recovery curves, exclude low quality data, perform data normalization, extract quantitative parameters, perform batch analysis and save the resulting data and figures for further use. Our tool is implemented as a single-screen Graphical User Interface (GUI) and is highly interactive, as it permits parameterization and visual data quality assessment at various points during the analysis.
easyFRAP is free software, available under the General Public License (GPL). Executable and source files, supplementary material and sample data sets can be downloaded at: ccl.med.upatras.gr/easyfrap.html. |
| doi_str_mv | 10.1093/bioinformatics/bts241 |
| format | Article |
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easyFRAP is free software, available under the General Public License (GPL). Executable and source files, supplementary material and sample data sets can be downloaded at: ccl.med.upatras.gr/easyfrap.html.</description><subject>Biological and medical sciences</subject><subject>Fluorescence Recovery After Photobleaching - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects)</subject><subject>Software</subject><subject>User-Computer Interface</subject><issn>1367-4803</issn><issn>1367-4811</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtLw0AUhQdRbK3-BGU2ggtj7zzyqLtSrAoFRXQdbiYzMJJk2sxE6L83obXF1X1951w4hFwzeGAwE9PCOtsY19YYrPLTIngu2QkZM5GkkcwYOz30IEbkwvtvAIghTs7JiPNYCpFkY4Ia_Xb5MX9_pNhQ2wTdogr2R9_T4RIFF3Ve0-BcRftvdNNhZQMORC8oh7kJxwVWW289dYYOnrTEgJfkzGDl9dW-TsjX8ulz8RKt3p5fF_NVpGTMQ5RoKJkpmESOQqbIZoYVaak4L1HzTJoCuEzjLDEGQCldcJ5hmbEUpOQ6VWJC7na-69ZtOu1DXluvdFVho13ncwac8wQgnfVovENV67xvtcnXra2x3fZQPqSb_08336Xb6272L7qi1uVB9RdnD9zuAfQKK9Nio6w_cgkISPlM_ALUqoji</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>RAPSOMANIKI, Maria Anna</creator><creator>KOTSANTIS, Panagiotis</creator><creator>SYMEONIDOU, Loanna-Eleni</creator><creator>GIAKOUMAKIS, Nickolaos-Nikiforos</creator><creator>TARAVIRAS, Stavros</creator><creator>LYGEROU, Zoi</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120701</creationdate><title>easyFRAP: an interactive, easy-to-use tool for qualitative and quantitative analysis of FRAP data</title><author>RAPSOMANIKI, Maria Anna ; KOTSANTIS, Panagiotis ; SYMEONIDOU, Loanna-Eleni ; GIAKOUMAKIS, Nickolaos-Nikiforos ; TARAVIRAS, Stavros ; LYGEROU, Zoi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-6e0d1fb14a2a347a19f1b7dc22dae284fb0247586ff00cceb228ad8170442e7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Biological and medical sciences</topic><topic>Fluorescence Recovery After Photobleaching - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects)</topic><topic>Software</topic><topic>User-Computer Interface</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RAPSOMANIKI, Maria Anna</creatorcontrib><creatorcontrib>KOTSANTIS, Panagiotis</creatorcontrib><creatorcontrib>SYMEONIDOU, Loanna-Eleni</creatorcontrib><creatorcontrib>GIAKOUMAKIS, Nickolaos-Nikiforos</creatorcontrib><creatorcontrib>TARAVIRAS, Stavros</creatorcontrib><creatorcontrib>LYGEROU, Zoi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioinformatics (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RAPSOMANIKI, Maria Anna</au><au>KOTSANTIS, Panagiotis</au><au>SYMEONIDOU, Loanna-Eleni</au><au>GIAKOUMAKIS, Nickolaos-Nikiforos</au><au>TARAVIRAS, Stavros</au><au>LYGEROU, Zoi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>easyFRAP: an interactive, easy-to-use tool for qualitative and quantitative analysis of FRAP data</atitle><jtitle>Bioinformatics (Oxford, England)</jtitle><addtitle>Bioinformatics</addtitle><date>2012-07-01</date><risdate>2012</risdate><volume>28</volume><issue>13</issue><spage>1800</spage><epage>1801</epage><pages>1800-1801</pages><issn>1367-4803</issn><eissn>1367-4811</eissn><abstract>We present easyFRAP, a versatile tool that assists quantitative and qualitative analysis of fluorescence recovery after photobleaching (FRAP) data. The user can handle simultaneously large data sets of raw data, visualize fluorescence recovery curves, exclude low quality data, perform data normalization, extract quantitative parameters, perform batch analysis and save the resulting data and figures for further use. Our tool is implemented as a single-screen Graphical User Interface (GUI) and is highly interactive, as it permits parameterization and visual data quality assessment at various points during the analysis.
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| source | MEDLINE; Oxford Open; PubMed Central; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Biological and medical sciences Fluorescence Recovery After Photobleaching - methods Fundamental and applied biological sciences. Psychology General aspects Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects) Software User-Computer Interface |
| title | easyFRAP: an interactive, easy-to-use tool for qualitative and quantitative analysis of FRAP data |
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