Improved Extraction of Saturated Fatty Acids but not Omega-3 Fatty Acids from Sheep Red Blood Cells Using a One-Step Extraction Procedure

Several methods are available to extract total lipid and methylate fatty acids from a range of samples including red blood cells (RBC). Fatty acid analysis of human RBC can be undertaken using a two-step extraction and methylation or a combined one-step extraction and methylation procedure. The lipi...

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Veröffentlicht in:	Lipids 2012-07, Vol.47 (7), p.719-727
Hauptverfasser:	Clayton, Edward H., Gulliver, Catherine E., Piltz, John W., Taylor, Robert D., Blake, Robert J., Meyer, Richard G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biomedical and Life Sciences Chloroform Erythrocytes Erythrocytes - chemistry Extraction FAME Fatty Acids - chemistry Fatty Acids - isolation & purification Fatty Acids, Omega-3 - chemistry Fatty Acids, Omega-3 - isolation & purification Fatty Acids, Omega-6 - chemistry Fatty Acids, Omega-6 - isolation & purification Life Sciences Lipidology Lipids Medical Biochemistry Medicinal Chemistry Methanol Methods Methylation Microbial Genetics and Genomics Neurochemistry Nutrition Omega‐3 Omega‐6 Polyunsaturated fatty acids Red blood cells Sheep Sheep, Domestic Toluene
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