Perfluorinated alginate for cellular encapsulation
Molecules of pentadecafluorooctanoyl chloride (PFC) were grafted onto alginate (Alg) using a linear poly(ethylene glycol) linker and amide bonds. The resulting Alg‐PFC material was characterized by proton nuclear magnetic resonance and infrared spectroscopies. The degree of PFC functionalization sig...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2012-08, Vol.100A (8), p.1963-1971 |
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| container_end_page | 1971 |
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| container_issue | 8 |
| container_start_page | 1963 |
| container_title | Journal of biomedical materials research. Part A |
| container_volume | 100A |
| creator | Gattás-Asfura, Kerim M. Fraker, Christopher A. Stabler, Cherie L. |
| description | Molecules of pentadecafluorooctanoyl chloride (PFC) were grafted onto alginate (Alg) using a linear poly(ethylene glycol) linker and amide bonds. The resulting Alg‐PFC material was characterized by proton nuclear magnetic resonance and infrared spectroscopies. The degree of PFC functionalization significantly influenced the physical and chemical properties of Alg‐PFC, particularly when the resulting polymer was ionically crosslinked into hydrogels. Alg‐PFC hydrogel beads fabricated via Ba2+ crosslinking were found to match the permeability properties of control alginate beads, except upon swelling over time in culture media. When used to encapsulate MIN6 cells, a beta cell line, Alg‐PFC beads demonstrated enhanced cell proliferation over alginate control beads. These results indicate that Alg‐PFC hydrogels retain some of the PFC's biological‐relevant benefits, such as enhancement of mass transport and bioinertness, to enhance cellular viability within alginate three‐dimensional hydrogel environments. We envision these functionalized hydrogels to be particularly useful in the encapsulation of cells with a high metabolic demand, such as pancreatic islets. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012. |
| doi_str_mv | 10.1002/jbm.a.34052 |
| format | Article |
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The resulting Alg‐PFC material was characterized by proton nuclear magnetic resonance and infrared spectroscopies. The degree of PFC functionalization significantly influenced the physical and chemical properties of Alg‐PFC, particularly when the resulting polymer was ionically crosslinked into hydrogels. Alg‐PFC hydrogel beads fabricated via Ba2+ crosslinking were found to match the permeability properties of control alginate beads, except upon swelling over time in culture media. When used to encapsulate MIN6 cells, a beta cell line, Alg‐PFC beads demonstrated enhanced cell proliferation over alginate control beads. These results indicate that Alg‐PFC hydrogels retain some of the PFC's biological‐relevant benefits, such as enhancement of mass transport and bioinertness, to enhance cellular viability within alginate three‐dimensional hydrogel environments. We envision these functionalized hydrogels to be particularly useful in the encapsulation of cells with a high metabolic demand, such as pancreatic islets. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.34052</identifier><identifier>PMID: 22544596</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>alginate ; Alginates - chemistry ; Alginates - pharmacology ; Animals ; Biological and medical sciences ; Cell Count ; Cell Line ; Cell Membrane Permeability - drug effects ; Cells, Immobilized - chemistry ; cellular encapsulation ; Dextrans - metabolism ; Diffusion - drug effects ; Fluorescein-5-isothiocyanate - analogs & derivatives ; Fluorescein-5-isothiocyanate - metabolism ; Glucuronic Acid - chemistry ; Glucuronic Acid - pharmacology ; Hexuronic Acids - chemistry ; Hexuronic Acids - pharmacology ; Hydrocarbons, Fluorinated - chemistry ; Hydrocarbons, Fluorinated - pharmacology ; Insulin-Secreting Cells - cytology ; Insulin-Secreting Cells - drug effects ; Magnetic Resonance Spectroscopy ; Medical sciences ; Mice ; oxygen ; Oxygen - metabolism ; perfluorocarbon ; Polyethylene Glycols - chemistry ; Spectroscopy, Fourier Transform Infrared ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments</subject><ispartof>Journal of biomedical materials research. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Molecules of pentadecafluorooctanoyl chloride (PFC) were grafted onto alginate (Alg) using a linear poly(ethylene glycol) linker and amide bonds. The resulting Alg‐PFC material was characterized by proton nuclear magnetic resonance and infrared spectroscopies. The degree of PFC functionalization significantly influenced the physical and chemical properties of Alg‐PFC, particularly when the resulting polymer was ionically crosslinked into hydrogels. Alg‐PFC hydrogel beads fabricated via Ba2+ crosslinking were found to match the permeability properties of control alginate beads, except upon swelling over time in culture media. When used to encapsulate MIN6 cells, a beta cell line, Alg‐PFC beads demonstrated enhanced cell proliferation over alginate control beads. These results indicate that Alg‐PFC hydrogels retain some of the PFC's biological‐relevant benefits, such as enhancement of mass transport and bioinertness, to enhance cellular viability within alginate three‐dimensional hydrogel environments. We envision these functionalized hydrogels to be particularly useful in the encapsulation of cells with a high metabolic demand, such as pancreatic islets. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.</description><subject>alginate</subject><subject>Alginates - chemistry</subject><subject>Alginates - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Cell Line</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Cells, Immobilized - chemistry</subject><subject>cellular encapsulation</subject><subject>Dextrans - metabolism</subject><subject>Diffusion - drug effects</subject><subject>Fluorescein-5-isothiocyanate - analogs & derivatives</subject><subject>Fluorescein-5-isothiocyanate - metabolism</subject><subject>Glucuronic Acid - chemistry</subject><subject>Glucuronic Acid - pharmacology</subject><subject>Hexuronic Acids - chemistry</subject><subject>Hexuronic Acids - pharmacology</subject><subject>Hydrocarbons, Fluorinated - chemistry</subject><subject>Hydrocarbons, Fluorinated - pharmacology</subject><subject>Insulin-Secreting Cells - cytology</subject><subject>Insulin-Secreting Cells - drug effects</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>oxygen</subject><subject>Oxygen - metabolism</subject><subject>perfluorocarbon</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90EtLxDAQB_Agiuvr5F16EQTpmnebo4pP1geiCF5CNp1I1267Ji3qtze7XfUmOWQIv5kJf4R2CR4SjOnRZDwdmiHjWNAVtEGEoClXUqzOa65SRpUcoM0QJhHLiNbRgFLBuVByA9F78K7qGl_WpoUiMdXrokpc4xMLVdVVxidQWzMLsWzLpt5Ga85UAXaW9xZ6Oj97PL1MR3cXV6fHo9QypWgKlhaE5TgeZYzLCpACMFFcqoICyR2WwhV5Hh8hY5ZLjAuLFcm5G4PBim2hg37uzDfvHYRWT8sw_5KpoemCJpgSlTPBZaSHPbW-CcGD0zNfTo3_ikjPQ9IxJG30IqSo95aDu_EUil_7k0oE-0tggjWV86a2ZfhzknAmMhYd6d1HWcHXfzv19cnNz_K07ylDC5-_Pca_aZmxTOjn2wv98sgyzB4yzdg3X-2MxA</recordid><startdate>201208</startdate><enddate>201208</enddate><creator>Gattás-Asfura, Kerim M.</creator><creator>Fraker, Christopher A.</creator><creator>Stabler, Cherie L.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201208</creationdate><title>Perfluorinated alginate for cellular encapsulation</title><author>Gattás-Asfura, Kerim M. ; Fraker, Christopher A. ; Stabler, Cherie L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3992-ec2d13808089aaf7de65e019469d2e18f065fd885e0e73c4600dc09184fbea093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>alginate</topic><topic>Alginates - chemistry</topic><topic>Alginates - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Cell Line</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>Cells, Immobilized - chemistry</topic><topic>cellular encapsulation</topic><topic>Dextrans - metabolism</topic><topic>Diffusion - drug effects</topic><topic>Fluorescein-5-isothiocyanate - analogs & derivatives</topic><topic>Fluorescein-5-isothiocyanate - metabolism</topic><topic>Glucuronic Acid - chemistry</topic><topic>Glucuronic Acid - pharmacology</topic><topic>Hexuronic Acids - chemistry</topic><topic>Hexuronic Acids - pharmacology</topic><topic>Hydrocarbons, Fluorinated - chemistry</topic><topic>Hydrocarbons, Fluorinated - pharmacology</topic><topic>Insulin-Secreting Cells - cytology</topic><topic>Insulin-Secreting Cells - drug effects</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>oxygen</topic><topic>Oxygen - metabolism</topic><topic>perfluorocarbon</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. Equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gattás-Asfura, Kerim M.</creatorcontrib><creatorcontrib>Fraker, Christopher A.</creatorcontrib><creatorcontrib>Stabler, Cherie L.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gattás-Asfura, Kerim M.</au><au>Fraker, Christopher A.</au><au>Stabler, Cherie L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Perfluorinated alginate for cellular encapsulation</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2012-08</date><risdate>2012</risdate><volume>100A</volume><issue>8</issue><spage>1963</spage><epage>1971</epage><pages>1963-1971</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>Molecules of pentadecafluorooctanoyl chloride (PFC) were grafted onto alginate (Alg) using a linear poly(ethylene glycol) linker and amide bonds. The resulting Alg‐PFC material was characterized by proton nuclear magnetic resonance and infrared spectroscopies. The degree of PFC functionalization significantly influenced the physical and chemical properties of Alg‐PFC, particularly when the resulting polymer was ionically crosslinked into hydrogels. Alg‐PFC hydrogel beads fabricated via Ba2+ crosslinking were found to match the permeability properties of control alginate beads, except upon swelling over time in culture media. When used to encapsulate MIN6 cells, a beta cell line, Alg‐PFC beads demonstrated enhanced cell proliferation over alginate control beads. These results indicate that Alg‐PFC hydrogels retain some of the PFC's biological‐relevant benefits, such as enhancement of mass transport and bioinertness, to enhance cellular viability within alginate three‐dimensional hydrogel environments. We envision these functionalized hydrogels to be particularly useful in the encapsulation of cells with a high metabolic demand, such as pancreatic islets. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 2012.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22544596</pmid><doi>10.1002/jbm.a.34052</doi><tpages>9</tpages></addata></record> |
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| subjects | alginate Alginates - chemistry Alginates - pharmacology Animals Biological and medical sciences Cell Count Cell Line Cell Membrane Permeability - drug effects Cells, Immobilized - chemistry cellular encapsulation Dextrans - metabolism Diffusion - drug effects Fluorescein-5-isothiocyanate - analogs & derivatives Fluorescein-5-isothiocyanate - metabolism Glucuronic Acid - chemistry Glucuronic Acid - pharmacology Hexuronic Acids - chemistry Hexuronic Acids - pharmacology Hydrocarbons, Fluorinated - chemistry Hydrocarbons, Fluorinated - pharmacology Insulin-Secreting Cells - cytology Insulin-Secreting Cells - drug effects Magnetic Resonance Spectroscopy Medical sciences Mice oxygen Oxygen - metabolism perfluorocarbon Polyethylene Glycols - chemistry Spectroscopy, Fourier Transform Infrared Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments |
| title | Perfluorinated alginate for cellular encapsulation |
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