Simultaneous analysis of multiple cytokines in the vitreous of patients with sarcoid uveitis
Levels of some cytokines are significantly higher in the vitreous fluid of patients with acute uveitis than in normal vitreous fluid. The authors sought to determine which proinflammatory cytokines were upregulated in the vitreous fluid of patients with ocular sarcoidosis. Samples of vitreous fluid...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2012-06, Vol.53 (7), p.3827-3833 |
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| container_title | Investigative ophthalmology & visual science |
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| creator | Nagata, Kenji Maruyama, Kazuichi Uno, Kazuko Shinomiya, Katsuhiko Yoneda, Kazuhito Hamuro, Junji Sugita, Sunao Yoshimura, Takeru Sonoda, Koh-Hei Mochizuki, Manabu Kinoshita, Shigeru |
| description | Levels of some cytokines are significantly higher in the vitreous fluid of patients with acute uveitis than in normal vitreous fluid. The authors sought to determine which proinflammatory cytokines were upregulated in the vitreous fluid of patients with ocular sarcoidosis.
Samples of vitreous fluid were collected from patients with sarcoid uveitis and from nonsarcoid control patients with idiopathic epiretinal membrane. The levels of 27 proinflammatory cytokines were measured with a multiplex beads array system. Postvitrectomy macular thickness was also measured by using spectral domain optical coherence tomography (SD-OCT). To assess the relationship between cytokine levels and disease stage, the authors divided patients into three groups based on macular thickness 1 month after operation.
The vitreous levels of 17 cytokines were significantly higher in patients with ocular sarcoidosis than in nonsarcoid controls. Serum levels of interferon γ-induced protein 10 (IP-10) were also higher in ocular sarcoidosis patients than in nonsarcoid controls. Conversely, serum levels of interleukin (IL) 15 in ocular sarcoidosis patients were lower than in the control group. Analysis of cytokine levels and macular thickness revealed that IL-1ra, IL-4, IL-8, IFN-γ, IP-10, monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein (MIP)-1β, and regulated on activation, normal T-cell expressed and secreted (RANTES) were significantly upregulated in patients with thin cystoid macular edema group.
Patients with ocular sarcoidosis had elevated levels of proinflammatory cytokines in vitreous fluids. Different cytokines might contribute to different stages of macular edema. |
| doi_str_mv | 10.1167/iovs.11-9244 |
| format | Article |
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Samples of vitreous fluid were collected from patients with sarcoid uveitis and from nonsarcoid control patients with idiopathic epiretinal membrane. The levels of 27 proinflammatory cytokines were measured with a multiplex beads array system. Postvitrectomy macular thickness was also measured by using spectral domain optical coherence tomography (SD-OCT). To assess the relationship between cytokine levels and disease stage, the authors divided patients into three groups based on macular thickness 1 month after operation.
The vitreous levels of 17 cytokines were significantly higher in patients with ocular sarcoidosis than in nonsarcoid controls. Serum levels of interferon γ-induced protein 10 (IP-10) were also higher in ocular sarcoidosis patients than in nonsarcoid controls. Conversely, serum levels of interleukin (IL) 15 in ocular sarcoidosis patients were lower than in the control group. Analysis of cytokine levels and macular thickness revealed that IL-1ra, IL-4, IL-8, IFN-γ, IP-10, monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein (MIP)-1β, and regulated on activation, normal T-cell expressed and secreted (RANTES) were significantly upregulated in patients with thin cystoid macular edema group.
Patients with ocular sarcoidosis had elevated levels of proinflammatory cytokines in vitreous fluids. Different cytokines might contribute to different stages of macular edema.</description><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.11-9244</identifier><identifier>PMID: 22589430</identifier><language>eng</language><publisher>United States</publisher><subject>Aged ; Case-Control Studies ; Chemokine CXCL10 - blood ; Cytokines - analysis ; Cytokines - blood ; Female ; Humans ; Interleukin-15 - blood ; Male ; Polymerase Chain Reaction ; Retina - pathology ; Sarcoidosis - immunology ; Tomography, Optical Coherence ; Uveitis - immunology ; Vitreous Body - chemistry</subject><ispartof>Investigative ophthalmology & visual science, 2012-06, Vol.53 (7), p.3827-3833</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c329t-7c30c19c5c1093c2614b74686722c8e79a94486b04ab73d796dbb00e67c3c1a43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22589430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nagata, Kenji</creatorcontrib><creatorcontrib>Maruyama, Kazuichi</creatorcontrib><creatorcontrib>Uno, Kazuko</creatorcontrib><creatorcontrib>Shinomiya, Katsuhiko</creatorcontrib><creatorcontrib>Yoneda, Kazuhito</creatorcontrib><creatorcontrib>Hamuro, Junji</creatorcontrib><creatorcontrib>Sugita, Sunao</creatorcontrib><creatorcontrib>Yoshimura, Takeru</creatorcontrib><creatorcontrib>Sonoda, Koh-Hei</creatorcontrib><creatorcontrib>Mochizuki, Manabu</creatorcontrib><creatorcontrib>Kinoshita, Shigeru</creatorcontrib><title>Simultaneous analysis of multiple cytokines in the vitreous of patients with sarcoid uveitis</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>Levels of some cytokines are significantly higher in the vitreous fluid of patients with acute uveitis than in normal vitreous fluid. The authors sought to determine which proinflammatory cytokines were upregulated in the vitreous fluid of patients with ocular sarcoidosis.
Samples of vitreous fluid were collected from patients with sarcoid uveitis and from nonsarcoid control patients with idiopathic epiretinal membrane. The levels of 27 proinflammatory cytokines were measured with a multiplex beads array system. Postvitrectomy macular thickness was also measured by using spectral domain optical coherence tomography (SD-OCT). To assess the relationship between cytokine levels and disease stage, the authors divided patients into three groups based on macular thickness 1 month after operation.
The vitreous levels of 17 cytokines were significantly higher in patients with ocular sarcoidosis than in nonsarcoid controls. Serum levels of interferon γ-induced protein 10 (IP-10) were also higher in ocular sarcoidosis patients than in nonsarcoid controls. Conversely, serum levels of interleukin (IL) 15 in ocular sarcoidosis patients were lower than in the control group. Analysis of cytokine levels and macular thickness revealed that IL-1ra, IL-4, IL-8, IFN-γ, IP-10, monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein (MIP)-1β, and regulated on activation, normal T-cell expressed and secreted (RANTES) were significantly upregulated in patients with thin cystoid macular edema group.
Patients with ocular sarcoidosis had elevated levels of proinflammatory cytokines in vitreous fluids. Different cytokines might contribute to different stages of macular edema.</description><subject>Aged</subject><subject>Case-Control Studies</subject><subject>Chemokine CXCL10 - blood</subject><subject>Cytokines - analysis</subject><subject>Cytokines - blood</subject><subject>Female</subject><subject>Humans</subject><subject>Interleukin-15 - blood</subject><subject>Male</subject><subject>Polymerase Chain Reaction</subject><subject>Retina - pathology</subject><subject>Sarcoidosis - immunology</subject><subject>Tomography, Optical Coherence</subject><subject>Uveitis - immunology</subject><subject>Vitreous Body - chemistry</subject><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkD1PwzAQhi0EoqWwMSOPDAT8lTgeUVU-pEoMwIYUOY6rGpI4-Jyi_nsSWhDTvTo990r3IHROyTWlmbxxfgNDShQT4gBNaZqyJJU5P_yXJ-gE4J0QRikjx2jCWJorwckUvT27pq-jbq3vAetW11twgP0Kj2vX1RabbfQfrrWAXYvj2uKNi-EHH6hOR2fbCPjLxTUGHYx3Fe431kUHp-hopWuwZ_s5Q693i5f5Q7J8un-c3y4Tw5mKiTScGKpMaihR3LCMilKKLM8kYya3UmklRJ6VROhS8kqqrCpLQmw2HBqqBZ-hy11vF_xnbyEWjQNj63r3VkGHx1XOxvYZutqhJniAYFdFF1yjw3aAitFnMfocUjH6HPCLfXNfNrb6g38F8m-X93JE</recordid><startdate>20120620</startdate><enddate>20120620</enddate><creator>Nagata, Kenji</creator><creator>Maruyama, Kazuichi</creator><creator>Uno, Kazuko</creator><creator>Shinomiya, Katsuhiko</creator><creator>Yoneda, Kazuhito</creator><creator>Hamuro, Junji</creator><creator>Sugita, Sunao</creator><creator>Yoshimura, Takeru</creator><creator>Sonoda, Koh-Hei</creator><creator>Mochizuki, Manabu</creator><creator>Kinoshita, Shigeru</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120620</creationdate><title>Simultaneous analysis of multiple cytokines in the vitreous of patients with sarcoid uveitis</title><author>Nagata, Kenji ; Maruyama, Kazuichi ; Uno, Kazuko ; Shinomiya, Katsuhiko ; Yoneda, Kazuhito ; Hamuro, Junji ; Sugita, Sunao ; Yoshimura, Takeru ; Sonoda, Koh-Hei ; Mochizuki, Manabu ; Kinoshita, Shigeru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-7c30c19c5c1093c2614b74686722c8e79a94486b04ab73d796dbb00e67c3c1a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aged</topic><topic>Case-Control Studies</topic><topic>Chemokine CXCL10 - blood</topic><topic>Cytokines - analysis</topic><topic>Cytokines - blood</topic><topic>Female</topic><topic>Humans</topic><topic>Interleukin-15 - blood</topic><topic>Male</topic><topic>Polymerase Chain Reaction</topic><topic>Retina - pathology</topic><topic>Sarcoidosis - immunology</topic><topic>Tomography, Optical Coherence</topic><topic>Uveitis - immunology</topic><topic>Vitreous Body - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagata, Kenji</creatorcontrib><creatorcontrib>Maruyama, Kazuichi</creatorcontrib><creatorcontrib>Uno, Kazuko</creatorcontrib><creatorcontrib>Shinomiya, Katsuhiko</creatorcontrib><creatorcontrib>Yoneda, Kazuhito</creatorcontrib><creatorcontrib>Hamuro, Junji</creatorcontrib><creatorcontrib>Sugita, Sunao</creatorcontrib><creatorcontrib>Yoshimura, Takeru</creatorcontrib><creatorcontrib>Sonoda, Koh-Hei</creatorcontrib><creatorcontrib>Mochizuki, Manabu</creatorcontrib><creatorcontrib>Kinoshita, Shigeru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagata, Kenji</au><au>Maruyama, Kazuichi</au><au>Uno, Kazuko</au><au>Shinomiya, Katsuhiko</au><au>Yoneda, Kazuhito</au><au>Hamuro, Junji</au><au>Sugita, Sunao</au><au>Yoshimura, Takeru</au><au>Sonoda, Koh-Hei</au><au>Mochizuki, Manabu</au><au>Kinoshita, Shigeru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous analysis of multiple cytokines in the vitreous of patients with sarcoid uveitis</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2012-06-20</date><risdate>2012</risdate><volume>53</volume><issue>7</issue><spage>3827</spage><epage>3833</epage><pages>3827-3833</pages><issn>1552-5783</issn><eissn>1552-5783</eissn><abstract>Levels of some cytokines are significantly higher in the vitreous fluid of patients with acute uveitis than in normal vitreous fluid. The authors sought to determine which proinflammatory cytokines were upregulated in the vitreous fluid of patients with ocular sarcoidosis.
Samples of vitreous fluid were collected from patients with sarcoid uveitis and from nonsarcoid control patients with idiopathic epiretinal membrane. The levels of 27 proinflammatory cytokines were measured with a multiplex beads array system. Postvitrectomy macular thickness was also measured by using spectral domain optical coherence tomography (SD-OCT). To assess the relationship between cytokine levels and disease stage, the authors divided patients into three groups based on macular thickness 1 month after operation.
The vitreous levels of 17 cytokines were significantly higher in patients with ocular sarcoidosis than in nonsarcoid controls. Serum levels of interferon γ-induced protein 10 (IP-10) were also higher in ocular sarcoidosis patients than in nonsarcoid controls. Conversely, serum levels of interleukin (IL) 15 in ocular sarcoidosis patients were lower than in the control group. Analysis of cytokine levels and macular thickness revealed that IL-1ra, IL-4, IL-8, IFN-γ, IP-10, monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein (MIP)-1β, and regulated on activation, normal T-cell expressed and secreted (RANTES) were significantly upregulated in patients with thin cystoid macular edema group.
Patients with ocular sarcoidosis had elevated levels of proinflammatory cytokines in vitreous fluids. Different cytokines might contribute to different stages of macular edema.</abstract><cop>United States</cop><pmid>22589430</pmid><doi>10.1167/iovs.11-9244</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aged Case-Control Studies Chemokine CXCL10 - blood Cytokines - analysis Cytokines - blood Female Humans Interleukin-15 - blood Male Polymerase Chain Reaction Retina - pathology Sarcoidosis - immunology Tomography, Optical Coherence Uveitis - immunology Vitreous Body - chemistry |
| title | Simultaneous analysis of multiple cytokines in the vitreous of patients with sarcoid uveitis |
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