Mass Production of Rubusoside Using a Novel Stevioside-Specific β-Glucosidase from Aspergillus aculeatus
Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method....
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2012-06, Vol.60 (24), p.6210-6216 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Ko, Jin-A Kim, Young-Min Ryu, Young Bae Jeong, Hyung Jae Park, Tae-Soon Park, Su-Jin Wee, Young-Jung Kim, Joong-Su Kim, Doman Lee, Woo Song |
| description | Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific β-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50–60 °C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by 1H and 13C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other β-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 μL of SSGase at pH 5.1 and 63 °C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries. |
| doi_str_mv | 10.1021/jf300531e |
| format | Article |
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However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific β-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50–60 °C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by 1H and 13C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other β-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 μL of SSGase at pH 5.1 and 63 °C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf300531e</identifier><identifier>PMID: 22530920</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Aspergillus - enzymology ; Aspergillus aculeatus ; beta-glucosidase ; Biological and medical sciences ; Cellulases - metabolism ; chromatography ; Diterpenes, Kaurane - biosynthesis ; Diterpenes, Kaurane - metabolism ; enzymatic hydrolysis ; Food additives ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; Glucosides - biosynthesis ; Glucosides - metabolism ; Hydrogen-Ion Concentration ; Hydrolysis ; Kinetics ; nuclear magnetic resonance spectroscopy ; pharmaceutical industry ; solubilization ; steviol ; stevioside ; Substrate Specificity</subject><ispartof>Journal of agricultural and food chemistry, 2012-06, Vol.60 (24), p.6210-6216</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a369t-786b72fdf90a3367c891b4be7cef9ff8d353195cbf2ca61cbce8f2ee8ac222b33</citedby><cites>FETCH-LOGICAL-a369t-786b72fdf90a3367c891b4be7cef9ff8d353195cbf2ca61cbce8f2ee8ac222b33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf300531e$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf300531e$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26019447$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22530920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ko, Jin-A</creatorcontrib><creatorcontrib>Kim, Young-Min</creatorcontrib><creatorcontrib>Ryu, Young Bae</creatorcontrib><creatorcontrib>Jeong, Hyung Jae</creatorcontrib><creatorcontrib>Park, Tae-Soon</creatorcontrib><creatorcontrib>Park, Su-Jin</creatorcontrib><creatorcontrib>Wee, Young-Jung</creatorcontrib><creatorcontrib>Kim, Joong-Su</creatorcontrib><creatorcontrib>Kim, Doman</creatorcontrib><creatorcontrib>Lee, Woo Song</creatorcontrib><title>Mass Production of Rubusoside Using a Novel Stevioside-Specific β-Glucosidase from Aspergillus aculeatus</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific β-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50–60 °C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by 1H and 13C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other β-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 μL of SSGase at pH 5.1 and 63 °C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries.</description><subject>Aspergillus - enzymology</subject><subject>Aspergillus aculeatus</subject><subject>beta-glucosidase</subject><subject>Biological and medical sciences</subject><subject>Cellulases - metabolism</subject><subject>chromatography</subject><subject>Diterpenes, Kaurane - biosynthesis</subject><subject>Diterpenes, Kaurane - metabolism</subject><subject>enzymatic hydrolysis</subject><subject>Food additives</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Glucosides - biosynthesis</subject><subject>Glucosides - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>Kinetics</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>pharmaceutical industry</subject><subject>solubilization</subject><subject>steviol</subject><subject>stevioside</subject><subject>Substrate Specificity</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0EtuFDEQBmALgcgksOAC4A1SWDT40c9lFJEQKTzEMOtWtbs88sjTHlztSFyLg-RMcTJDsmFVkuvTL9fP2BspPkqh5KeN1UJUWuIztpCVEkUlZfucLUReFm1VyyN2TLQRQrRVI16yI6UqLTolFsx9BSL-I4YxmdmFiQfLf6YhUSA3Il-Rm9Yc-Ldwg54vZ7xxD4tiuUPjrDP89m9x6ZO5fwVCbmPY8jPaYVw77xNxMMkjzIlesRcWPOHrwzxhq4vPv86_FNffL6_Oz64L0HU3F01bD42yo-0EaF03pu3kUA7YGLSdte2o86FdZQarDNTSDAZbqxBbMEqpQesTdrrP3cXwOyHN_daRQe9hwpCov2-srJSu60w_7KmJgSii7XfRbSH-yejB9Y_NZvv2EJuGLY6P8l-VGbw_ACAD3kaYjKMnVwvZlWWT3bu9sxB6WMdsVkslZCmElI1q9FMSGOo3IcUp9_WfL90BdweXrQ</recordid><startdate>20120620</startdate><enddate>20120620</enddate><creator>Ko, Jin-A</creator><creator>Kim, Young-Min</creator><creator>Ryu, Young Bae</creator><creator>Jeong, Hyung Jae</creator><creator>Park, Tae-Soon</creator><creator>Park, Su-Jin</creator><creator>Wee, Young-Jung</creator><creator>Kim, Joong-Su</creator><creator>Kim, Doman</creator><creator>Lee, Woo Song</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120620</creationdate><title>Mass Production of Rubusoside Using a Novel Stevioside-Specific β-Glucosidase from Aspergillus aculeatus</title><author>Ko, Jin-A ; Kim, Young-Min ; Ryu, Young Bae ; Jeong, Hyung Jae ; Park, Tae-Soon ; Park, Su-Jin ; Wee, Young-Jung ; Kim, Joong-Su ; Kim, Doman ; Lee, Woo Song</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a369t-786b72fdf90a3367c891b4be7cef9ff8d353195cbf2ca61cbce8f2ee8ac222b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Aspergillus - enzymology</topic><topic>Aspergillus aculeatus</topic><topic>beta-glucosidase</topic><topic>Biological and medical sciences</topic><topic>Cellulases - metabolism</topic><topic>chromatography</topic><topic>Diterpenes, Kaurane - biosynthesis</topic><topic>Diterpenes, Kaurane - metabolism</topic><topic>enzymatic hydrolysis</topic><topic>Food additives</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Glucosides - biosynthesis</topic><topic>Glucosides - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydrolysis</topic><topic>Kinetics</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>pharmaceutical industry</topic><topic>solubilization</topic><topic>steviol</topic><topic>stevioside</topic><topic>Substrate Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ko, Jin-A</creatorcontrib><creatorcontrib>Kim, Young-Min</creatorcontrib><creatorcontrib>Ryu, Young Bae</creatorcontrib><creatorcontrib>Jeong, Hyung Jae</creatorcontrib><creatorcontrib>Park, Tae-Soon</creatorcontrib><creatorcontrib>Park, Su-Jin</creatorcontrib><creatorcontrib>Wee, Young-Jung</creatorcontrib><creatorcontrib>Kim, Joong-Su</creatorcontrib><creatorcontrib>Kim, Doman</creatorcontrib><creatorcontrib>Lee, Woo Song</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ko, Jin-A</au><au>Kim, Young-Min</au><au>Ryu, Young Bae</au><au>Jeong, Hyung Jae</au><au>Park, Tae-Soon</au><au>Park, Su-Jin</au><au>Wee, Young-Jung</au><au>Kim, Joong-Su</au><au>Kim, Doman</au><au>Lee, Woo Song</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mass Production of Rubusoside Using a Novel Stevioside-Specific β-Glucosidase from Aspergillus aculeatus</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2012-06-20</date><risdate>2012</risdate><volume>60</volume><issue>24</issue><spage>6210</spage><epage>6216</epage><pages>6210-6216</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Rubusoside (R) is a natural sweetener and a solubilizing agent with antiangiogenic and antiallergic properties. However, currently, its production is quite expensive, and therefore, we have investigated nine commercially available glycosidases to optimize an economically viable R-production method. A stevioside (ST)-specific β-glucosidase (SSGase) was selected and purified 7-fold from Aspergillus aculeatus Viscozyme L by a two-step column chromatography procedure. The 79 kDa protein was stable from pH 3.0 to pH 7.0 at 50–60 °C. Hydrolysis of ST by SSGase produced R and steviol monoglucosyl ester as determined by 1H and 13C nuclear magnetic resonance (NMR). Importantly, SSGase showed higher activity toward ST than other β-linked glucobioses. The optimal conditions for R production were 280 mM ST and 16.6 μL of SSGase at pH 5.1 and 63 °C. This is the first discussion detailing the production of R by enzymatic hydrolysis of ST and is useful for the food additive and pharmaceutical industries.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>22530920</pmid><doi>10.1021/jf300531e</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2012-06, Vol.60 (24), p.6210-6216 |
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| subjects | Aspergillus - enzymology Aspergillus aculeatus beta-glucosidase Biological and medical sciences Cellulases - metabolism chromatography Diterpenes, Kaurane - biosynthesis Diterpenes, Kaurane - metabolism enzymatic hydrolysis Food additives Food industries Fundamental and applied biological sciences. Psychology General aspects Glucosides - biosynthesis Glucosides - metabolism Hydrogen-Ion Concentration Hydrolysis Kinetics nuclear magnetic resonance spectroscopy pharmaceutical industry solubilization steviol stevioside Substrate Specificity |
| title | Mass Production of Rubusoside Using a Novel Stevioside-Specific β-Glucosidase from Aspergillus aculeatus |
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