Effectiveness of tissue microarray technique for the assessment of estrogen and progesterone receptors expression in endometrioid endometrial cancer--preliminary report
To assess the effectiveness of the donor-block biopsies with a 2 mm-size needle in endometrioid endometrial cancer (EEC) in the tissue microarray (TMA) technique and the application of the TMA for estrogen receptors (ER) and progesterone receptors (PR) expression in EEC. The study examined EEC tissu...
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| Veröffentlicht in: | Ginekologia polska 2012-05, Vol.83 (5), p.342-346 |
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| creator | Gottwald, Leszek Sek, Piotr Kubiak, Robert Pasz-Walczak, Grazyna Piekarski, Janusz Szwalski, Jarosław Suzin, Jacek Tyliński, Wiesław Hendzel, Katarzyna Jeziorski, Arkadiusz |
| description | To assess the effectiveness of the donor-block biopsies with a 2 mm-size needle in endometrioid endometrial cancer (EEC) in the tissue microarray (TMA) technique and the application of the TMA for estrogen receptors (ER) and progesterone receptors (PR) expression in EEC.
The study examined EEC tissues from 60 patients. Tissue cores, 2 mm in size, in duplicate, were taken from the formalin-fixed and paraffin-embedded tissue donor blocks and constructed into the TMA recipient block. The presence of EEC tissue in the TMAs was analyzed, and the ER and PR expressions were examined.
EEC tissue in TMAs was confirmed in 56 cases (93.33%). In 49 of them (81.67%), both cores presented with cancer tissues. In 4 cases (6.67%) EEC tissue was absent. All cases with ECC present on the TMA slides were appropriate for the ER and PR analysis. In 29 EEC cases (51.98%) both ER and PR were expressed. In 3 cases (5.36%) only ER was expressed, in 8 cases (14.29%) only PR was expressed, and in 16 cases (28.57%) ER and PR were assessed as negative.
Two 2 mm-sized tissue cores from donor-block biopsies constructed into the TMA recipient block were sufficient to diagnose EEC and enabled the assessment of ER and PR expression in 93.3% of the cases. The use of the described TMA technique makes the immunohistochemical study of EEC easier and more time-efficient. |
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The study examined EEC tissues from 60 patients. Tissue cores, 2 mm in size, in duplicate, were taken from the formalin-fixed and paraffin-embedded tissue donor blocks and constructed into the TMA recipient block. The presence of EEC tissue in the TMAs was analyzed, and the ER and PR expressions were examined.
EEC tissue in TMAs was confirmed in 56 cases (93.33%). In 49 of them (81.67%), both cores presented with cancer tissues. In 4 cases (6.67%) EEC tissue was absent. All cases with ECC present on the TMA slides were appropriate for the ER and PR analysis. In 29 EEC cases (51.98%) both ER and PR were expressed. In 3 cases (5.36%) only ER was expressed, in 8 cases (14.29%) only PR was expressed, and in 16 cases (28.57%) ER and PR were assessed as negative.
Two 2 mm-sized tissue cores from donor-block biopsies constructed into the TMA recipient block were sufficient to diagnose EEC and enabled the assessment of ER and PR expression in 93.3% of the cases. The use of the described TMA technique makes the immunohistochemical study of EEC easier and more time-efficient.</description><identifier>ISSN: 0017-0011</identifier><identifier>EISSN: 2543-6767</identifier><identifier>PMID: 22708330</identifier><language>pol</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Adult ; Biomarkers, Tumor - metabolism ; Biopsy ; Carcinoma, Endometrioid - metabolism ; Carcinoma, Endometrioid - pathology ; Endometrial cancer ; Endometrial Neoplasms - metabolism ; Endometrial Neoplasms - pathology ; Female ; Humans ; Immunohistochemistry ; Middle Aged ; Receptors, Estrogen - metabolism ; Receptors, Progesterone - metabolism ; Reproducibility of Results ; Tissue Array Analysis - methods</subject><ispartof>Ginekologia polska, 2012-05, Vol.83 (5), p.342-346</ispartof><rights>2012. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22708330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gottwald, Leszek</creatorcontrib><creatorcontrib>Sek, Piotr</creatorcontrib><creatorcontrib>Kubiak, Robert</creatorcontrib><creatorcontrib>Pasz-Walczak, Grazyna</creatorcontrib><creatorcontrib>Piekarski, Janusz</creatorcontrib><creatorcontrib>Szwalski, Jarosław</creatorcontrib><creatorcontrib>Suzin, Jacek</creatorcontrib><creatorcontrib>Tyliński, Wiesław</creatorcontrib><creatorcontrib>Hendzel, Katarzyna</creatorcontrib><creatorcontrib>Jeziorski, Arkadiusz</creatorcontrib><title>Effectiveness of tissue microarray technique for the assessment of estrogen and progesterone receptors expression in endometrioid endometrial cancer--preliminary report</title><title>Ginekologia polska</title><addtitle>Ginekol Pol</addtitle><description>To assess the effectiveness of the donor-block biopsies with a 2 mm-size needle in endometrioid endometrial cancer (EEC) in the tissue microarray (TMA) technique and the application of the TMA for estrogen receptors (ER) and progesterone receptors (PR) expression in EEC.
The study examined EEC tissues from 60 patients. Tissue cores, 2 mm in size, in duplicate, were taken from the formalin-fixed and paraffin-embedded tissue donor blocks and constructed into the TMA recipient block. The presence of EEC tissue in the TMAs was analyzed, and the ER and PR expressions were examined.
EEC tissue in TMAs was confirmed in 56 cases (93.33%). In 49 of them (81.67%), both cores presented with cancer tissues. In 4 cases (6.67%) EEC tissue was absent. All cases with ECC present on the TMA slides were appropriate for the ER and PR analysis. In 29 EEC cases (51.98%) both ER and PR were expressed. In 3 cases (5.36%) only ER was expressed, in 8 cases (14.29%) only PR was expressed, and in 16 cases (28.57%) ER and PR were assessed as negative.
Two 2 mm-sized tissue cores from donor-block biopsies constructed into the TMA recipient block were sufficient to diagnose EEC and enabled the assessment of ER and PR expression in 93.3% of the cases. The use of the described TMA technique makes the immunohistochemical study of EEC easier and more time-efficient.</description><subject>Adult</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Biopsy</subject><subject>Carcinoma, Endometrioid - metabolism</subject><subject>Carcinoma, Endometrioid - pathology</subject><subject>Endometrial cancer</subject><subject>Endometrial Neoplasms - metabolism</subject><subject>Endometrial Neoplasms - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Middle Aged</subject><subject>Receptors, Estrogen - metabolism</subject><subject>Receptors, Progesterone - metabolism</subject><subject>Reproducibility of Results</subject><subject>Tissue Array Analysis - methods</subject><issn>0017-0011</issn><issn>2543-6767</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkUtLAzEQx4MottR-BQl48bKQx26SHqXUBxS86HnJZmdtym6yJlmx38iPaYoVwTnMi98M82fO0JxVJS-EFPIczQmhssiOztAyxj3JJphkq9UlmjEmieKczNHXpuvAJPsBDmLEvsPJxjgBHqwJXoegDziB2Tn7npudDzjtAOsYMz2AS8cJiCn4N3BYuxaPxzQmCN4BDmBgTD5EDJ9jyCPWO2wdBtf6AVKw3rZ_he6x0c5AKIoM93awTodDXjL6kK7QRaf7CMtTXKDX-83L-rHYPj88re-2xUjFKhXKtB03XJeVUoyzRpRE0gb0qiOsog1XUhBOSkqBE1CVKFVlNK1aqkA00FV8gW5_9mYhWXJM9WCjgb7XDvwUa0oYZYIQKTN68w_d-ym4fF3NSlEyShU_UtcnamoGaOsx2CHLqn9_wL8BV3OIeA</recordid><startdate>20120501</startdate><enddate>20120501</enddate><creator>Gottwald, Leszek</creator><creator>Sek, Piotr</creator><creator>Kubiak, Robert</creator><creator>Pasz-Walczak, Grazyna</creator><creator>Piekarski, Janusz</creator><creator>Szwalski, Jarosław</creator><creator>Suzin, Jacek</creator><creator>Tyliński, Wiesław</creator><creator>Hendzel, Katarzyna</creator><creator>Jeziorski, Arkadiusz</creator><general>Wydawnictwo Via Medica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20120501</creationdate><title>Effectiveness of tissue microarray technique for the assessment of estrogen and progesterone receptors expression in endometrioid endometrial cancer--preliminary report</title><author>Gottwald, Leszek ; Sek, Piotr ; Kubiak, Robert ; Pasz-Walczak, Grazyna ; Piekarski, Janusz ; Szwalski, Jarosław ; Suzin, Jacek ; Tyliński, Wiesław ; Hendzel, Katarzyna ; Jeziorski, Arkadiusz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p169t-8cdf3c3a4588232b64071bea9f0251b3876030411e30e856485ca15d18e6bef53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>pol</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Biopsy</topic><topic>Carcinoma, Endometrioid - metabolism</topic><topic>Carcinoma, Endometrioid - pathology</topic><topic>Endometrial cancer</topic><topic>Endometrial Neoplasms - metabolism</topic><topic>Endometrial Neoplasms - pathology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Middle Aged</topic><topic>Receptors, Estrogen - metabolism</topic><topic>Receptors, Progesterone - metabolism</topic><topic>Reproducibility of Results</topic><topic>Tissue Array Analysis - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gottwald, Leszek</creatorcontrib><creatorcontrib>Sek, Piotr</creatorcontrib><creatorcontrib>Kubiak, Robert</creatorcontrib><creatorcontrib>Pasz-Walczak, Grazyna</creatorcontrib><creatorcontrib>Piekarski, Janusz</creatorcontrib><creatorcontrib>Szwalski, Jarosław</creatorcontrib><creatorcontrib>Suzin, Jacek</creatorcontrib><creatorcontrib>Tyliński, Wiesław</creatorcontrib><creatorcontrib>Hendzel, Katarzyna</creatorcontrib><creatorcontrib>Jeziorski, Arkadiusz</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Ginekologia polska</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gottwald, Leszek</au><au>Sek, Piotr</au><au>Kubiak, Robert</au><au>Pasz-Walczak, Grazyna</au><au>Piekarski, Janusz</au><au>Szwalski, Jarosław</au><au>Suzin, Jacek</au><au>Tyliński, Wiesław</au><au>Hendzel, Katarzyna</au><au>Jeziorski, Arkadiusz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effectiveness of tissue microarray technique for the assessment of estrogen and progesterone receptors expression in endometrioid endometrial cancer--preliminary report</atitle><jtitle>Ginekologia polska</jtitle><addtitle>Ginekol Pol</addtitle><date>2012-05-01</date><risdate>2012</risdate><volume>83</volume><issue>5</issue><spage>342</spage><epage>346</epage><pages>342-346</pages><issn>0017-0011</issn><eissn>2543-6767</eissn><abstract>To assess the effectiveness of the donor-block biopsies with a 2 mm-size needle in endometrioid endometrial cancer (EEC) in the tissue microarray (TMA) technique and the application of the TMA for estrogen receptors (ER) and progesterone receptors (PR) expression in EEC.
The study examined EEC tissues from 60 patients. Tissue cores, 2 mm in size, in duplicate, were taken from the formalin-fixed and paraffin-embedded tissue donor blocks and constructed into the TMA recipient block. The presence of EEC tissue in the TMAs was analyzed, and the ER and PR expressions were examined.
EEC tissue in TMAs was confirmed in 56 cases (93.33%). In 49 of them (81.67%), both cores presented with cancer tissues. In 4 cases (6.67%) EEC tissue was absent. All cases with ECC present on the TMA slides were appropriate for the ER and PR analysis. In 29 EEC cases (51.98%) both ER and PR were expressed. In 3 cases (5.36%) only ER was expressed, in 8 cases (14.29%) only PR was expressed, and in 16 cases (28.57%) ER and PR were assessed as negative.
Two 2 mm-sized tissue cores from donor-block biopsies constructed into the TMA recipient block were sufficient to diagnose EEC and enabled the assessment of ER and PR expression in 93.3% of the cases. The use of the described TMA technique makes the immunohistochemical study of EEC easier and more time-efficient.</abstract><cop>Poland</cop><pub>Wydawnictwo Via Medica</pub><pmid>22708330</pmid><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Biomarkers, Tumor - metabolism Biopsy Carcinoma, Endometrioid - metabolism Carcinoma, Endometrioid - pathology Endometrial cancer Endometrial Neoplasms - metabolism Endometrial Neoplasms - pathology Female Humans Immunohistochemistry Middle Aged Receptors, Estrogen - metabolism Receptors, Progesterone - metabolism Reproducibility of Results Tissue Array Analysis - methods |
| title | Effectiveness of tissue microarray technique for the assessment of estrogen and progesterone receptors expression in endometrioid endometrial cancer--preliminary report |
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