The effect of single-stranded DNA binding proteins on template/primer-independent DNA synthesis in the presence of nicking endonuclease Nt.BspD6I

Nt.BspD6I nicking endonuclease stimulates template/primer-independent DNA synthesis by Bst DNA polymerase. Template/primer-independent DNA synthesis may be one of the reasons for the formation of nonspecific products in certain DNA amplification reactions, especially those involving nicking endonucl...

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Veröffentlicht in:	Russian journal of bioorganic chemistry 2012-03, Vol.38 (2), p.171-176
Hauptverfasser:	Zyrina, N. V., Artyukh, R. I., Svad’bina, I. V., Zheleznaya, L. A., Matvienko, N. I.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry DNA biosynthesis DNA-binding protein DNA-directed DNA polymerase Endonuclease Escherichia coli Life Sciences Nicking Organic Chemistry Phages single-stranded DNA-binding protein
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