Directed evolution of ([beta][alpha]) sub(8)-barrel enzymes: establishing phosphoribosylanthranilate isomerisation activity on the scaffold of the tryptophan synthase [alpha]-subunit

Phosphoribosylanthranilate (PRA) isomerase (TrpF) and tryptophan synthase alpha -subunit (TrpA) are ( beta alpha ) sub(8)-barrel enzymes that are involved in the biosynthesis of tryptophan. They contain a conserved phosphate binding site, which indicates a common evolutionary origin. In order to exp...

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