The Sox17-mCherry fusion mouse line allows visualization of endoderm and vascular endothelial development
Sox17 is a HMG‐box transcription factor that has been shown to play important roles in both cardio‐vascular development and endoderm formation. To analyze these processes in greater detail, we have generated a Sox17‐mCherry fusion (SCF) protein by gene targeting in ES cells. SCF reporter mice are ho...
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| Veröffentlicht in: | Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2012-06, Vol.50 (6), p.496-505 |
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| container_title | Genesis (New York, N.Y. : 2000) |
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| creator | Burtscher, Ingo Barkey, Wenke Schwarzfischer, Michael Theis, Fabian J. Lickert, Heiko |
| description | Sox17 is a HMG‐box transcription factor that has been shown to play important roles in both cardio‐vascular development and endoderm formation. To analyze these processes in greater detail, we have generated a Sox17‐mCherry fusion (SCF) protein by gene targeting in ES cells. SCF reporter mice are homozygous viable and faithfully reflect the endogenous Sox17 protein localization. We report that SCF positive cells constitute a subpopulation in the visceral endoderm before gastrulation and time‐lapse imaging reveals that SCF monitors the nascent definitive endoderm during epithelialization. After gastrulation, SCF marks the mid‐ and hindgut endoderm and vascular endothelial network, which can be imaged during establishment in allantois explant cultures. The SCF reporter is downregulated in the endoderm epithelium and upregulated in endothelial cells of the intestine, lung, and pancreas during organogenesis. In summary, the generation of the Sox17‐mCherry reporter mouse line allows direct visualization of endoderm and vascular development in culture and the mouse embryo. genesis 50:496–505, 2012. © 2011 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/dvg.20829 |
| format | Article |
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To analyze these processes in greater detail, we have generated a Sox17‐mCherry fusion (SCF) protein by gene targeting in ES cells. SCF reporter mice are homozygous viable and faithfully reflect the endogenous Sox17 protein localization. We report that SCF positive cells constitute a subpopulation in the visceral endoderm before gastrulation and time‐lapse imaging reveals that SCF monitors the nascent definitive endoderm during epithelialization. After gastrulation, SCF marks the mid‐ and hindgut endoderm and vascular endothelial network, which can be imaged during establishment in allantois explant cultures. The SCF reporter is downregulated in the endoderm epithelium and upregulated in endothelial cells of the intestine, lung, and pancreas during organogenesis. 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To analyze these processes in greater detail, we have generated a Sox17‐mCherry fusion (SCF) protein by gene targeting in ES cells. SCF reporter mice are homozygous viable and faithfully reflect the endogenous Sox17 protein localization. We report that SCF positive cells constitute a subpopulation in the visceral endoderm before gastrulation and time‐lapse imaging reveals that SCF monitors the nascent definitive endoderm during epithelialization. After gastrulation, SCF marks the mid‐ and hindgut endoderm and vascular endothelial network, which can be imaged during establishment in allantois explant cultures. The SCF reporter is downregulated in the endoderm epithelium and upregulated in endothelial cells of the intestine, lung, and pancreas during organogenesis. In summary, the generation of the Sox17‐mCherry reporter mouse line allows direct visualization of endoderm and vascular development in culture and the mouse embryo. genesis 50:496–505, 2012. © 2011 Wiley Periodicals, Inc.</description><subject>Animals</subject><subject>Cell Differentiation - genetics</subject><subject>Cells, Cultured</subject><subject>Embryonic Stem Cells</subject><subject>endoderm</subject><subject>Endoderm - embryology</subject><subject>Endothelium, Vascular - embryology</subject><subject>ES cells</subject><subject>Founder Effect</subject><subject>Foxa2</subject><subject>gastrulation</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Targeting</subject><subject>Genotype</subject><subject>gut</subject><subject>HMGB Proteins - genetics</subject><subject>live imaging</subject><subject>Luminescent Proteins - genetics</subject><subject>lung</subject><subject>mCherry fluorescent reporter</subject><subject>Mice</subject><subject>mouse embryos</subject><subject>pancreas</subject><subject>Recombinant Fusion Proteins</subject><subject>Red Fluorescent Protein</subject><subject>Sox17</subject><subject>SOXF Transcription Factors - genetics</subject><subject>vasculature</subject><issn>1526-954X</issn><issn>1526-968X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1P3DAQhi3UCihw4A9UPraHgD_y5SPatmlVVA4sHzdrkoxZFyfe2snC9tc3y7LceprRzDOvNA8hp5ydccbEebt6OBOsFGqPHPJM5InKy_t3uz5L7w_Ihxh_M8ayUoh9ciAEF5zz8pDY-QLptX_mRdLNFhjCmpoxWt_Tzo8RqbM9UnDOP0W6snEEZ__CsNl7Q7FvfYuho9C3dAWxGR2El-mwQGfB0RZX6Pyyw344Ju8NuIgnr_WI3Hz7Op99Ty6vqh-zi8ukSYVSieGcQWOKulQAEtqsyTKVGik4TBNZ1I3MVarSYoJkLXLFOBrVcqWaHEyt5BH5tM1dBv9nxDjozsYGnYMep5c0Z5MqKUu1QT9v0Sb4GAMavQy2g7CeIL0xqyez-sXsxH58jR3rDts3cqdyAs63wJN1uP5_kv5yW-0ik-2FjQM-v11AeNR5IYtM3_2qdHVXzn9WKddS_gON8JNj</recordid><startdate>201206</startdate><enddate>201206</enddate><creator>Burtscher, Ingo</creator><creator>Barkey, Wenke</creator><creator>Schwarzfischer, Michael</creator><creator>Theis, Fabian J.</creator><creator>Lickert, Heiko</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201206</creationdate><title>The Sox17-mCherry fusion mouse line allows visualization of endoderm and vascular endothelial development</title><author>Burtscher, Ingo ; Barkey, Wenke ; Schwarzfischer, Michael ; Theis, Fabian J. ; Lickert, Heiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4299-f110acf7b89aa3ad5c5594f321a89a37bc3694947acf3b26901ef9d199c6afb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Cell Differentiation - genetics</topic><topic>Cells, Cultured</topic><topic>Embryonic Stem Cells</topic><topic>endoderm</topic><topic>Endoderm - embryology</topic><topic>Endothelium, Vascular - embryology</topic><topic>ES cells</topic><topic>Founder Effect</topic><topic>Foxa2</topic><topic>gastrulation</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Targeting</topic><topic>Genotype</topic><topic>gut</topic><topic>HMGB Proteins - genetics</topic><topic>live imaging</topic><topic>Luminescent Proteins - genetics</topic><topic>lung</topic><topic>mCherry fluorescent reporter</topic><topic>Mice</topic><topic>mouse embryos</topic><topic>pancreas</topic><topic>Recombinant Fusion Proteins</topic><topic>Red Fluorescent Protein</topic><topic>Sox17</topic><topic>SOXF Transcription Factors - genetics</topic><topic>vasculature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burtscher, Ingo</creatorcontrib><creatorcontrib>Barkey, Wenke</creatorcontrib><creatorcontrib>Schwarzfischer, Michael</creatorcontrib><creatorcontrib>Theis, Fabian J.</creatorcontrib><creatorcontrib>Lickert, Heiko</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genesis (New York, N.Y. : 2000)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burtscher, Ingo</au><au>Barkey, Wenke</au><au>Schwarzfischer, Michael</au><au>Theis, Fabian J.</au><au>Lickert, Heiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Sox17-mCherry fusion mouse line allows visualization of endoderm and vascular endothelial development</atitle><jtitle>Genesis (New York, N.Y. : 2000)</jtitle><addtitle>Genesis</addtitle><date>2012-06</date><risdate>2012</risdate><volume>50</volume><issue>6</issue><spage>496</spage><epage>505</epage><pages>496-505</pages><issn>1526-954X</issn><eissn>1526-968X</eissn><abstract>Sox17 is a HMG‐box transcription factor that has been shown to play important roles in both cardio‐vascular development and endoderm formation. To analyze these processes in greater detail, we have generated a Sox17‐mCherry fusion (SCF) protein by gene targeting in ES cells. SCF reporter mice are homozygous viable and faithfully reflect the endogenous Sox17 protein localization. We report that SCF positive cells constitute a subpopulation in the visceral endoderm before gastrulation and time‐lapse imaging reveals that SCF monitors the nascent definitive endoderm during epithelialization. After gastrulation, SCF marks the mid‐ and hindgut endoderm and vascular endothelial network, which can be imaged during establishment in allantois explant cultures. The SCF reporter is downregulated in the endoderm epithelium and upregulated in endothelial cells of the intestine, lung, and pancreas during organogenesis. 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| subjects | Animals Cell Differentiation - genetics Cells, Cultured Embryonic Stem Cells endoderm Endoderm - embryology Endothelium, Vascular - embryology ES cells Founder Effect Foxa2 gastrulation Gene Expression Regulation, Developmental Gene Targeting Genotype gut HMGB Proteins - genetics live imaging Luminescent Proteins - genetics lung mCherry fluorescent reporter Mice mouse embryos pancreas Recombinant Fusion Proteins Red Fluorescent Protein Sox17 SOXF Transcription Factors - genetics vasculature |
| title | The Sox17-mCherry fusion mouse line allows visualization of endoderm and vascular endothelial development |
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