Transcriptional analysis of orange-spotted grouper reacting to experimental grouper iridovirus infection
► Grouper virus responsive genes (VRGs) were selected by cDNA microarray. ► Expression profiles of VRGs responded to GIV, LPS and PIC were established. ► Transcriptional expressions of VRGs were also observed after GIV immunization. ► VRGs may play important roles in teleost adaptive immunity in ant...
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| Veröffentlicht in: | Developmental and comparative immunology 2012-06, Vol.37 (2), p.233-242 |
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| creator | Wu, Ming-Shan Chen, Chien-Wen Liu, Yu-Cheng Huang, Hseng-Hsiang Lin, Chih-Hung Tzeng, Chyng-Shyan Chang, Chi-Yao |
| description | ► Grouper virus responsive genes (VRGs) were selected by cDNA microarray. ► Expression profiles of VRGs responded to GIV, LPS and PIC were established. ► Transcriptional expressions of VRGs were also observed after GIV immunization. ► VRGs may play important roles in teleost adaptive immunity in antiviral protection.
Disease caused by grouper iridovirus (GIV) has resulted in economic losses due to high mortality in grouper culture. Thirty-eight up- and 48 down-regulated known entities have been identified using a GIV-infected grouper kidney cDNA microarray chip. Further quantitative validation was executed in the head-kidney and spleen for 24 candidate genes and 7 immune factors following GIV inoculation. Significant induction with various patterns could be seen in 30 tested genes in the spleen. However, only 23 genes had induction in the head-kidney and meanwhile 5 genes showed reduction. Transcriptional expression profiles of selected genes in response to lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid (PIC) were also established to compare with the GIV-stimulated expression. The results indicated that the responses of most genes facing GIV invasion have more similarities to PIC treatment than LPS. Seven genes are thought to be interferon-related factors: RNA helicase DHX58, ISG15, viperin, HECT E3 ligase (HECT), CD9, urokinase plasminogen activator surface receptor (PLAUR) and Mx-1. Following immunization with inactivated GIV, significant induction could be seen in DHX58, viperin, IL-1β, IL-8, COX-2, HECT, PLAUR, IgM, Mx-1, very large inducible GTPase-1 (VLIG1) and TNF-α in the head-kidney or spleen, and the latter 6 genes also had a gradual increasing pattern by a boosting immunization. These factors might play important roles in adaptive antiviral protection. Thus, we have characterized the temporal response patterns of virus responsive genes and have also identified several potential immune markers to further investigate host antiviral defense mechanisms. |
| doi_str_mv | 10.1016/j.dci.2012.04.002 |
| format | Article |
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Disease caused by grouper iridovirus (GIV) has resulted in economic losses due to high mortality in grouper culture. Thirty-eight up- and 48 down-regulated known entities have been identified using a GIV-infected grouper kidney cDNA microarray chip. Further quantitative validation was executed in the head-kidney and spleen for 24 candidate genes and 7 immune factors following GIV inoculation. Significant induction with various patterns could be seen in 30 tested genes in the spleen. However, only 23 genes had induction in the head-kidney and meanwhile 5 genes showed reduction. Transcriptional expression profiles of selected genes in response to lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid (PIC) were also established to compare with the GIV-stimulated expression. The results indicated that the responses of most genes facing GIV invasion have more similarities to PIC treatment than LPS. Seven genes are thought to be interferon-related factors: RNA helicase DHX58, ISG15, viperin, HECT E3 ligase (HECT), CD9, urokinase plasminogen activator surface receptor (PLAUR) and Mx-1. Following immunization with inactivated GIV, significant induction could be seen in DHX58, viperin, IL-1β, IL-8, COX-2, HECT, PLAUR, IgM, Mx-1, very large inducible GTPase-1 (VLIG1) and TNF-α in the head-kidney or spleen, and the latter 6 genes also had a gradual increasing pattern by a boosting immunization. These factors might play important roles in adaptive antiviral protection. Thus, we have characterized the temporal response patterns of virus responsive genes and have also identified several potential immune markers to further investigate host antiviral defense mechanisms.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2012.04.002</identifier><identifier>PMID: 22504162</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Animals ; complementary DNA ; defense mechanisms ; DNA Virus Infections - genetics ; DNA Virus Infections - immunology ; DNA Virus Infections - veterinary ; Epinephelus coioides ; financial economics ; Fish Diseases - genetics ; Fish Diseases - immunology ; Gene Expression Profiling ; gene expression regulation ; genes ; Grouper ; Head Kidney - immunology ; Immunization ; immunoglobulin M ; interleukin-1beta ; interleukin-8 ; Iridovirus ; kidneys ; lipopolysaccharides ; Microarray ; microarray technology ; mortality ; Perciformes ; Quantitative PCR ; Ranavirus ; RNA helicases ; spleen ; transcription (genetics) ; tumor necrosis factor-alpha ; u-plasminogen activator ; ubiquitin-protein ligase ; viruses</subject><ispartof>Developmental and comparative immunology, 2012-06, Vol.37 (2), p.233-242</ispartof><rights>2012 Elsevier Ltd</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-6f89ea90725e2f0329e66e4927ce9a35d1f04fc9d40e2b9f9c65e6219369bf3a3</citedby><cites>FETCH-LOGICAL-c420t-6f89ea90725e2f0329e66e4927ce9a35d1f04fc9d40e2b9f9c65e6219369bf3a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0145305X12000730$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22504162$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Ming-Shan</creatorcontrib><creatorcontrib>Chen, Chien-Wen</creatorcontrib><creatorcontrib>Liu, Yu-Cheng</creatorcontrib><creatorcontrib>Huang, Hseng-Hsiang</creatorcontrib><creatorcontrib>Lin, Chih-Hung</creatorcontrib><creatorcontrib>Tzeng, Chyng-Shyan</creatorcontrib><creatorcontrib>Chang, Chi-Yao</creatorcontrib><title>Transcriptional analysis of orange-spotted grouper reacting to experimental grouper iridovirus infection</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>► Grouper virus responsive genes (VRGs) were selected by cDNA microarray. ► Expression profiles of VRGs responded to GIV, LPS and PIC were established. ► Transcriptional expressions of VRGs were also observed after GIV immunization. ► VRGs may play important roles in teleost adaptive immunity in antiviral protection.
Disease caused by grouper iridovirus (GIV) has resulted in economic losses due to high mortality in grouper culture. Thirty-eight up- and 48 down-regulated known entities have been identified using a GIV-infected grouper kidney cDNA microarray chip. Further quantitative validation was executed in the head-kidney and spleen for 24 candidate genes and 7 immune factors following GIV inoculation. Significant induction with various patterns could be seen in 30 tested genes in the spleen. However, only 23 genes had induction in the head-kidney and meanwhile 5 genes showed reduction. Transcriptional expression profiles of selected genes in response to lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid (PIC) were also established to compare with the GIV-stimulated expression. The results indicated that the responses of most genes facing GIV invasion have more similarities to PIC treatment than LPS. Seven genes are thought to be interferon-related factors: RNA helicase DHX58, ISG15, viperin, HECT E3 ligase (HECT), CD9, urokinase plasminogen activator surface receptor (PLAUR) and Mx-1. Following immunization with inactivated GIV, significant induction could be seen in DHX58, viperin, IL-1β, IL-8, COX-2, HECT, PLAUR, IgM, Mx-1, very large inducible GTPase-1 (VLIG1) and TNF-α in the head-kidney or spleen, and the latter 6 genes also had a gradual increasing pattern by a boosting immunization. These factors might play important roles in adaptive antiviral protection. Thus, we have characterized the temporal response patterns of virus responsive genes and have also identified several potential immune markers to further investigate host antiviral defense mechanisms.</description><subject>Animals</subject><subject>complementary DNA</subject><subject>defense mechanisms</subject><subject>DNA Virus Infections - genetics</subject><subject>DNA Virus Infections - immunology</subject><subject>DNA Virus Infections - veterinary</subject><subject>Epinephelus coioides</subject><subject>financial economics</subject><subject>Fish Diseases - genetics</subject><subject>Fish Diseases - immunology</subject><subject>Gene Expression Profiling</subject><subject>gene expression regulation</subject><subject>genes</subject><subject>Grouper</subject><subject>Head Kidney - immunology</subject><subject>Immunization</subject><subject>immunoglobulin M</subject><subject>interleukin-1beta</subject><subject>interleukin-8</subject><subject>Iridovirus</subject><subject>kidneys</subject><subject>lipopolysaccharides</subject><subject>Microarray</subject><subject>microarray technology</subject><subject>mortality</subject><subject>Perciformes</subject><subject>Quantitative PCR</subject><subject>Ranavirus</subject><subject>RNA helicases</subject><subject>spleen</subject><subject>transcription (genetics)</subject><subject>tumor necrosis factor-alpha</subject><subject>u-plasminogen activator</subject><subject>ubiquitin-protein ligase</subject><subject>viruses</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFv2yAYhtG0aU3b_YBdOh97sfeBMTHaaaq6tVKkHtZKvSGCP1KixHhgV82_3xcl2bEcQILnffXxMPaVQ8WBq-_rqnOhEsBFBbICEB_YjLdzXQK0-iObAZdNWUPzfMbOc14DrZbDZ3YmRAOSKzFjL4_J9tmlMIwh9nZTWNp2OeQi-iLS2wrLPMRxxK5YpTgNmIqE1o2hXxVjLPCNbsIW-5GyJyCk0MXXkKZchN6j21dfsk_ebjJ-OZ4X7OnX7ePNXbl4-H1_83NROilgLJVvNVoNc9Gg8FALjUqh1GLuUNu66bgH6Z3uJKBYaq-dalAJrmull7629QW7PvQOKf6dMI9mG7LDzcb2GKdsOJAvzaWoCeUH1KWYc0JvBvqKTTuCzF6wWRsSbPaCDUhDgilzdayfllvs_idORgn4dgC8jcauUsjm6Q81KJIvNZ-3RPw4EEgaXgMmk13A3mEXErkyXQzvDPAP4aOW4Q</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Wu, Ming-Shan</creator><creator>Chen, Chien-Wen</creator><creator>Liu, Yu-Cheng</creator><creator>Huang, Hseng-Hsiang</creator><creator>Lin, Chih-Hung</creator><creator>Tzeng, Chyng-Shyan</creator><creator>Chang, Chi-Yao</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20120601</creationdate><title>Transcriptional analysis of orange-spotted grouper reacting to experimental grouper iridovirus infection</title><author>Wu, Ming-Shan ; Chen, Chien-Wen ; Liu, Yu-Cheng ; Huang, Hseng-Hsiang ; Lin, Chih-Hung ; Tzeng, Chyng-Shyan ; Chang, Chi-Yao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-6f89ea90725e2f0329e66e4927ce9a35d1f04fc9d40e2b9f9c65e6219369bf3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>complementary DNA</topic><topic>defense mechanisms</topic><topic>DNA Virus Infections - genetics</topic><topic>DNA Virus Infections - immunology</topic><topic>DNA Virus Infections - veterinary</topic><topic>Epinephelus coioides</topic><topic>financial economics</topic><topic>Fish Diseases - genetics</topic><topic>Fish Diseases - immunology</topic><topic>Gene Expression Profiling</topic><topic>gene expression regulation</topic><topic>genes</topic><topic>Grouper</topic><topic>Head Kidney - immunology</topic><topic>Immunization</topic><topic>immunoglobulin M</topic><topic>interleukin-1beta</topic><topic>interleukin-8</topic><topic>Iridovirus</topic><topic>kidneys</topic><topic>lipopolysaccharides</topic><topic>Microarray</topic><topic>microarray technology</topic><topic>mortality</topic><topic>Perciformes</topic><topic>Quantitative PCR</topic><topic>Ranavirus</topic><topic>RNA helicases</topic><topic>spleen</topic><topic>transcription (genetics)</topic><topic>tumor necrosis factor-alpha</topic><topic>u-plasminogen activator</topic><topic>ubiquitin-protein ligase</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Ming-Shan</creatorcontrib><creatorcontrib>Chen, Chien-Wen</creatorcontrib><creatorcontrib>Liu, Yu-Cheng</creatorcontrib><creatorcontrib>Huang, Hseng-Hsiang</creatorcontrib><creatorcontrib>Lin, Chih-Hung</creatorcontrib><creatorcontrib>Tzeng, Chyng-Shyan</creatorcontrib><creatorcontrib>Chang, Chi-Yao</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Ming-Shan</au><au>Chen, Chien-Wen</au><au>Liu, Yu-Cheng</au><au>Huang, Hseng-Hsiang</au><au>Lin, Chih-Hung</au><au>Tzeng, Chyng-Shyan</au><au>Chang, Chi-Yao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional analysis of orange-spotted grouper reacting to experimental grouper iridovirus infection</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>37</volume><issue>2</issue><spage>233</spage><epage>242</epage><pages>233-242</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>► Grouper virus responsive genes (VRGs) were selected by cDNA microarray. ► Expression profiles of VRGs responded to GIV, LPS and PIC were established. ► Transcriptional expressions of VRGs were also observed after GIV immunization. ► VRGs may play important roles in teleost adaptive immunity in antiviral protection.
Disease caused by grouper iridovirus (GIV) has resulted in economic losses due to high mortality in grouper culture. Thirty-eight up- and 48 down-regulated known entities have been identified using a GIV-infected grouper kidney cDNA microarray chip. Further quantitative validation was executed in the head-kidney and spleen for 24 candidate genes and 7 immune factors following GIV inoculation. Significant induction with various patterns could be seen in 30 tested genes in the spleen. However, only 23 genes had induction in the head-kidney and meanwhile 5 genes showed reduction. Transcriptional expression profiles of selected genes in response to lipopolysaccharide (LPS) or polyinosinic:polycytidylic acid (PIC) were also established to compare with the GIV-stimulated expression. The results indicated that the responses of most genes facing GIV invasion have more similarities to PIC treatment than LPS. Seven genes are thought to be interferon-related factors: RNA helicase DHX58, ISG15, viperin, HECT E3 ligase (HECT), CD9, urokinase plasminogen activator surface receptor (PLAUR) and Mx-1. Following immunization with inactivated GIV, significant induction could be seen in DHX58, viperin, IL-1β, IL-8, COX-2, HECT, PLAUR, IgM, Mx-1, very large inducible GTPase-1 (VLIG1) and TNF-α in the head-kidney or spleen, and the latter 6 genes also had a gradual increasing pattern by a boosting immunization. These factors might play important roles in adaptive antiviral protection. Thus, we have characterized the temporal response patterns of virus responsive genes and have also identified several potential immune markers to further investigate host antiviral defense mechanisms.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>22504162</pmid><doi>10.1016/j.dci.2012.04.002</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals complementary DNA defense mechanisms DNA Virus Infections - genetics DNA Virus Infections - immunology DNA Virus Infections - veterinary Epinephelus coioides financial economics Fish Diseases - genetics Fish Diseases - immunology Gene Expression Profiling gene expression regulation genes Grouper Head Kidney - immunology Immunization immunoglobulin M interleukin-1beta interleukin-8 Iridovirus kidneys lipopolysaccharides Microarray microarray technology mortality Perciformes Quantitative PCR Ranavirus RNA helicases spleen transcription (genetics) tumor necrosis factor-alpha u-plasminogen activator ubiquitin-protein ligase viruses |
| title | Transcriptional analysis of orange-spotted grouper reacting to experimental grouper iridovirus infection |
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