Effects of Lipopolysaccharide Biosynthesis Mutations on K1 Polysaccharide Association with the Escherichia coli Cell Surface

The presence of cell-bound K1 capsule and K1 polysaccharide in culture supernatants was determined in a series of in-frame nonpolar core biosynthetic mutants from Escherichia coli KT1094 (K1, R1 core lipopolysaccharide [LPS] type) for which the major core oligosaccharide structures were determined....

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Veröffentlicht in:	Journal of Bacteriology 2012-07, Vol.194 (13), p.3356-3367
Hauptverfasser:	Jiménez, Natalia, Senchenkova, Sofya N, Knirel, Yuriy A, Pieretti, Giuseppina, Corsaro, Maria M, Aquilini, Eleonora, Regué, Miguel, Merino, Susana, Tomás, Juan M
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, Bacterial Bacterial Capsules - chemistry Bacterial Capsules - metabolism Bacteriology Biosynthesis Carbohydrates Cell culture Cell Membrane - chemistry Cell Membrane - metabolism chromatography Culture Media, Conditioned - chemistry E coli EDTA (chelating agent) Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - metabolism Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism genes Heptoses - chemistry lipopolysaccharides Lipopolysaccharides - biosynthesis Lipopolysaccharides - chemistry Magnetic Resonance Spectroscopy mutants Mutation nuclear magnetic resonance spectroscopy O Antigens - genetics O Antigens - metabolism oligosaccharides Polysaccharides, Bacterial Spectrometry, Mass, Electrospray Ionization
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