Enhanced kinase sensing by radio-phosphorylation and oriented immobilization of a GBP-linked kinase substrate on a gold surface

A new strategy for enhanced sensing of kinases was developed using radio-phosphorylation and a polypeptide with an affinity for gold that was linked directly to kinase substrates. Gold-binding polypeptide (GBP)-linked GST-fusion substrates were efficiently immobilized on gold-coated slides, which is...

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Veröffentlicht in:	Sensors and actuators. B, Chemical Chemical, 2011-12, Vol.160 (1), p.632-636
Hauptverfasser:	Rho, Jong Kook, Kang, Jung Ae, Choi, Mi Hee, Ko, Kyong-Cheol, Park, Sang Hyun
Format:	Artikel
Sprache:	eng
Schlagworte:	Actuators Biochip Detection Gold Gold-binding peptide Immobilization Kinases microarray technology Oriented immobilization Phosphorylation Polypeptides protein kinases Proteins Radioisotope detection Strategy
Online-Zugang:	Volltext
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