CD23+ CD21(high) CD1d(high) B cells in inflamed lymph nodes are a locally differentiated population with increased antigen capture and activation potential
CD23(+)CD21(high)CD1d(high) B cells in inflamed nodes (Bin cells) accumulate in the lymph nodes (LNs) draining inflamed joints of the TNF-α-transgenic mouse model of rheumatoid arthritis and are primarily involved in the significant histological and functional LN alterations that accompany disease e...
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Veröffentlicht in: | The Journal of immunology (1950) 2012-06, Vol.188 (12), p.5944-5953 |
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creator | Moshkani, Safiehkhatoon Kuzin, Igor I Adewale, Funmilola Jansson, Johan Sanz, Iñaki Schwarz, Edward M Bottaro, Andrea |
description | CD23(+)CD21(high)CD1d(high) B cells in inflamed nodes (Bin cells) accumulate in the lymph nodes (LNs) draining inflamed joints of the TNF-α-transgenic mouse model of rheumatoid arthritis and are primarily involved in the significant histological and functional LN alterations that accompany disease exacerbation in this strain. In this study, we investigate the origin and function of Bin cells. We show that adoptively transferred GFP(+) sorted mature follicular B (FoB) cells home preferentially to inflamed LNs of TNF-α-transgenic mice where they rapidly differentiate into Bin cells, with a close correlation with the endogenous Bin fraction. Bin cells are also induced in wild-type LNs after immunization with T-dependent Ags and display a germinal center phenotype at higher rates compared with FoB cells. Furthermore, we show that Bin cells can capture and process Ag-immune complexes in a CD21-dependent manner more efficiently than can FoB cells, and they express greater levels of MHC class II and costimulatory Ags CD80 and CD86. We propose that Bin cells are a previously unrecognized inflammation-induced B cell population with increased Ag capture and activation potential, which may facilitate normal immune responses but may contribute to autoimmunity when chronic inflammation causes their accumulation and persistence in affected LNs. |
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In this study, we investigate the origin and function of Bin cells. We show that adoptively transferred GFP(+) sorted mature follicular B (FoB) cells home preferentially to inflamed LNs of TNF-α-transgenic mice where they rapidly differentiate into Bin cells, with a close correlation with the endogenous Bin fraction. Bin cells are also induced in wild-type LNs after immunization with T-dependent Ags and display a germinal center phenotype at higher rates compared with FoB cells. Furthermore, we show that Bin cells can capture and process Ag-immune complexes in a CD21-dependent manner more efficiently than can FoB cells, and they express greater levels of MHC class II and costimulatory Ags CD80 and CD86. We propose that Bin cells are a previously unrecognized inflammation-induced B cell population with increased Ag capture and activation potential, which may facilitate normal immune responses but may contribute to autoimmunity when chronic inflammation causes their accumulation and persistence in affected LNs.</description><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.1103071</identifier><identifier>PMID: 22593620</identifier><language>eng</language><publisher>United States</publisher><subject>Adoptive Transfer ; Animals ; Antigen Presentation - immunology ; Antigens, CD1d - immunology ; Antigens, CD1d - metabolism ; Arthritis, Experimental - immunology ; Arthritis, Rheumatoid - immunology ; B-Lymphocyte Subsets - cytology ; B-Lymphocyte Subsets - immunology ; B-Lymphocyte Subsets - metabolism ; B-Lymphocytes - cytology ; B-Lymphocytes - immunology ; B-Lymphocytes - metabolism ; Cell Differentiation - immunology ; Disease Models, Animal ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Inflammation - immunology ; Inflammation - metabolism ; Lymph Nodes - cytology ; Lymph Nodes - immunology ; Lymphocyte Activation - immunology ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Receptors, Complement 3d - immunology ; Receptors, Complement 3d - metabolism ; Receptors, IgE - immunology ; Receptors, IgE - metabolism</subject><ispartof>The Journal of immunology (1950), 2012-06, Vol.188 (12), p.5944-5953</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22593620$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moshkani, Safiehkhatoon</creatorcontrib><creatorcontrib>Kuzin, Igor I</creatorcontrib><creatorcontrib>Adewale, Funmilola</creatorcontrib><creatorcontrib>Jansson, Johan</creatorcontrib><creatorcontrib>Sanz, Iñaki</creatorcontrib><creatorcontrib>Schwarz, Edward M</creatorcontrib><creatorcontrib>Bottaro, Andrea</creatorcontrib><title>CD23+ CD21(high) CD1d(high) B cells in inflamed lymph nodes are a locally differentiated population with increased antigen capture and activation potential</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>CD23(+)CD21(high)CD1d(high) B cells in inflamed nodes (Bin cells) accumulate in the lymph nodes (LNs) draining inflamed joints of the TNF-α-transgenic mouse model of rheumatoid arthritis and are primarily involved in the significant histological and functional LN alterations that accompany disease exacerbation in this strain. In this study, we investigate the origin and function of Bin cells. We show that adoptively transferred GFP(+) sorted mature follicular B (FoB) cells home preferentially to inflamed LNs of TNF-α-transgenic mice where they rapidly differentiate into Bin cells, with a close correlation with the endogenous Bin fraction. Bin cells are also induced in wild-type LNs after immunization with T-dependent Ags and display a germinal center phenotype at higher rates compared with FoB cells. Furthermore, we show that Bin cells can capture and process Ag-immune complexes in a CD21-dependent manner more efficiently than can FoB cells, and they express greater levels of MHC class II and costimulatory Ags CD80 and CD86. We propose that Bin cells are a previously unrecognized inflammation-induced B cell population with increased Ag capture and activation potential, which may facilitate normal immune responses but may contribute to autoimmunity when chronic inflammation causes their accumulation and persistence in affected LNs.</description><subject>Adoptive Transfer</subject><subject>Animals</subject><subject>Antigen Presentation - immunology</subject><subject>Antigens, CD1d - immunology</subject><subject>Antigens, CD1d - metabolism</subject><subject>Arthritis, Experimental - immunology</subject><subject>Arthritis, Rheumatoid - immunology</subject><subject>B-Lymphocyte Subsets - cytology</subject><subject>B-Lymphocyte Subsets - immunology</subject><subject>B-Lymphocyte Subsets - metabolism</subject><subject>B-Lymphocytes - cytology</subject><subject>B-Lymphocytes - immunology</subject><subject>B-Lymphocytes - metabolism</subject><subject>Cell Differentiation - immunology</subject><subject>Disease Models, Animal</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Flow Cytometry</subject><subject>Inflammation - immunology</subject><subject>Inflammation - metabolism</subject><subject>Lymph Nodes - cytology</subject><subject>Lymph Nodes - immunology</subject><subject>Lymphocyte Activation - immunology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Transgenic</subject><subject>Receptors, Complement 3d - immunology</subject><subject>Receptors, Complement 3d - metabolism</subject><subject>Receptors, IgE - immunology</subject><subject>Receptors, IgE - metabolism</subject><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtOwzAQRS0kREthzwp5WYQC40eceAnlKVViA-vISZzWlROH2AH1W_hZXFqk0Z2rmaMrzSB0QeCGA5e3G9O2Y-fsDSHAICNHaErSFBIhQEzQqfcbABBA-QmaUJpKJihM0c_igbJrHJXM12a1voqW1Ad7jyttrcemi9VY1eoa223br3Hnau2xGjRW2LpKWbvFtWkaPeguGBUi2Lt-tCoY1-FvE9YxoRq08nGjIrLSHa5UH8ZdRBdnVTBfe7p34S_EnqHjRlmvzw99hj6eHt8XL8ny7fl1cbdMekpISBoAxjKodSaBl01Z6lTmeZQmp0JQShpQXOWEySpjkmvQWc25KKWkZaYqwmZovs_tB_c5ah-K1vjd5arTbvQFASIFyTMpInp5QMcyfqPoB9OqYVv8P5T9AlYed4A</recordid><startdate>20120615</startdate><enddate>20120615</enddate><creator>Moshkani, Safiehkhatoon</creator><creator>Kuzin, Igor I</creator><creator>Adewale, Funmilola</creator><creator>Jansson, Johan</creator><creator>Sanz, Iñaki</creator><creator>Schwarz, Edward M</creator><creator>Bottaro, Andrea</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20120615</creationdate><title>CD23+ CD21(high) CD1d(high) B cells in inflamed lymph nodes are a locally differentiated population with increased antigen capture and activation potential</title><author>Moshkani, Safiehkhatoon ; Kuzin, Igor I ; Adewale, Funmilola ; Jansson, Johan ; Sanz, Iñaki ; Schwarz, Edward M ; Bottaro, Andrea</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-f003370de7904bfbbe5988e59f8266221f0a4a8139c7394e0e7d446b992b7ac13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adoptive Transfer</topic><topic>Animals</topic><topic>Antigen Presentation - immunology</topic><topic>Antigens, CD1d - immunology</topic><topic>Antigens, CD1d - metabolism</topic><topic>Arthritis, Experimental - immunology</topic><topic>Arthritis, Rheumatoid - immunology</topic><topic>B-Lymphocyte Subsets - cytology</topic><topic>B-Lymphocyte Subsets - immunology</topic><topic>B-Lymphocyte Subsets - metabolism</topic><topic>B-Lymphocytes - cytology</topic><topic>B-Lymphocytes - immunology</topic><topic>B-Lymphocytes - metabolism</topic><topic>Cell Differentiation - immunology</topic><topic>Disease Models, Animal</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Flow Cytometry</topic><topic>Inflammation - immunology</topic><topic>Inflammation - metabolism</topic><topic>Lymph Nodes - cytology</topic><topic>Lymph Nodes - immunology</topic><topic>Lymphocyte Activation - immunology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Transgenic</topic><topic>Receptors, Complement 3d - immunology</topic><topic>Receptors, Complement 3d - metabolism</topic><topic>Receptors, IgE - immunology</topic><topic>Receptors, IgE - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moshkani, Safiehkhatoon</creatorcontrib><creatorcontrib>Kuzin, Igor I</creatorcontrib><creatorcontrib>Adewale, Funmilola</creatorcontrib><creatorcontrib>Jansson, Johan</creatorcontrib><creatorcontrib>Sanz, Iñaki</creatorcontrib><creatorcontrib>Schwarz, Edward M</creatorcontrib><creatorcontrib>Bottaro, Andrea</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moshkani, Safiehkhatoon</au><au>Kuzin, Igor I</au><au>Adewale, Funmilola</au><au>Jansson, Johan</au><au>Sanz, Iñaki</au><au>Schwarz, Edward M</au><au>Bottaro, Andrea</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD23+ CD21(high) CD1d(high) B cells in inflamed lymph nodes are a locally differentiated population with increased antigen capture and activation potential</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2012-06-15</date><risdate>2012</risdate><volume>188</volume><issue>12</issue><spage>5944</spage><epage>5953</epage><pages>5944-5953</pages><eissn>1550-6606</eissn><abstract>CD23(+)CD21(high)CD1d(high) B cells in inflamed nodes (Bin cells) accumulate in the lymph nodes (LNs) draining inflamed joints of the TNF-α-transgenic mouse model of rheumatoid arthritis and are primarily involved in the significant histological and functional LN alterations that accompany disease exacerbation in this strain. In this study, we investigate the origin and function of Bin cells. We show that adoptively transferred GFP(+) sorted mature follicular B (FoB) cells home preferentially to inflamed LNs of TNF-α-transgenic mice where they rapidly differentiate into Bin cells, with a close correlation with the endogenous Bin fraction. Bin cells are also induced in wild-type LNs after immunization with T-dependent Ags and display a germinal center phenotype at higher rates compared with FoB cells. Furthermore, we show that Bin cells can capture and process Ag-immune complexes in a CD21-dependent manner more efficiently than can FoB cells, and they express greater levels of MHC class II and costimulatory Ags CD80 and CD86. We propose that Bin cells are a previously unrecognized inflammation-induced B cell population with increased Ag capture and activation potential, which may facilitate normal immune responses but may contribute to autoimmunity when chronic inflammation causes their accumulation and persistence in affected LNs.</abstract><cop>United States</cop><pmid>22593620</pmid><doi>10.4049/jimmunol.1103071</doi><tpages>10</tpages></addata></record> |
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subjects | Adoptive Transfer Animals Antigen Presentation - immunology Antigens, CD1d - immunology Antigens, CD1d - metabolism Arthritis, Experimental - immunology Arthritis, Rheumatoid - immunology B-Lymphocyte Subsets - cytology B-Lymphocyte Subsets - immunology B-Lymphocyte Subsets - metabolism B-Lymphocytes - cytology B-Lymphocytes - immunology B-Lymphocytes - metabolism Cell Differentiation - immunology Disease Models, Animal Enzyme-Linked Immunosorbent Assay Flow Cytometry Inflammation - immunology Inflammation - metabolism Lymph Nodes - cytology Lymph Nodes - immunology Lymphocyte Activation - immunology Mice Mice, Inbred C57BL Mice, Transgenic Receptors, Complement 3d - immunology Receptors, Complement 3d - metabolism Receptors, IgE - immunology Receptors, IgE - metabolism |
title | CD23+ CD21(high) CD1d(high) B cells in inflamed lymph nodes are a locally differentiated population with increased antigen capture and activation potential |
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