Low-intensity laser irradiation at 660 nm stimulates cytochrome c oxidase in stressed fibroblast cells
Background and Objective Low‐intensity laser irradiation (LILI) has been used to modulate a variety of biological processes, including diabetic wound healing. The mechanism of action is thought to exist primarily with the mitochondria. This study aimed to determine the effect of irradiation on norma...
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| Veröffentlicht in: | Lasers in surgery and medicine 2012-07, Vol.44 (5), p.429-434 |
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| creator | Houreld, Nicolette N. Masha, Roland T. Abrahamse, Heidi |
| description | Background and Objective
Low‐intensity laser irradiation (LILI) has been used to modulate a variety of biological processes, including diabetic wound healing. The mechanism of action is thought to exist primarily with the mitochondria. This study aimed to determine the effect of irradiation on normal, diabetic, and ischemic mitochondrial electron transport chain (ETC) complexes.
Materials and Methods
Normal, diabetic and ischemic human skin fibroblast mitochondria were irradiated in vitro at a wavelength of 660 nm and a fluence of either 5 or 15 J/cm2. Non‐irradiated mitochondria served as controls. Enzyme activities of mitochondrial complexes I, II, III, and IV were determined immediately post‐irradiation. Normal, diabetic, and ischemic cells were irradiated and adenosine triphosphate (ATP) and active mitochondria were determined by luminescence and fluorescent microscopy, respectively.
Results
Irradiated diabetic mitochondria at a fluence of 15 J/cm2 showed a significant decrease in complex III activity (P |
| doi_str_mv | 10.1002/lsm.22027 |
| format | Article |
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Low‐intensity laser irradiation (LILI) has been used to modulate a variety of biological processes, including diabetic wound healing. The mechanism of action is thought to exist primarily with the mitochondria. This study aimed to determine the effect of irradiation on normal, diabetic, and ischemic mitochondrial electron transport chain (ETC) complexes.
Materials and Methods
Normal, diabetic and ischemic human skin fibroblast mitochondria were irradiated in vitro at a wavelength of 660 nm and a fluence of either 5 or 15 J/cm2. Non‐irradiated mitochondria served as controls. Enzyme activities of mitochondrial complexes I, II, III, and IV were determined immediately post‐irradiation. Normal, diabetic, and ischemic cells were irradiated and adenosine triphosphate (ATP) and active mitochondria were determined by luminescence and fluorescent microscopy, respectively.
Results
Irradiated diabetic mitochondria at a fluence of 15 J/cm2 showed a significant decrease in complex III activity (P < 0.05). Normal (P < 0.01) and diabetic (P < 0.05) mitochondria irradiated at either 5 or 15 J/cm2 showed a significant increase in complex IV activity. ATP results showed a significant increase in irradiated normal cells (5 J/cm2; P < 0.05) and diabetic cells (15 J/cm2; P < 0.01). There was a higher accumulation of active mitochondria in irradiated cells than non‐irradiated cells.
Conclusion
Irradiation at 660 nm has the ability to influence mitochondrial enzyme activity, in particular cytochrome c oxidase. This leads to increased mitochondrial activity and ATP synthesis. Lasers Surg. Med. 44: 429–434, 2012. © 2012 Wiley Periodicals, Inc.</description><identifier>ISSN: 0196-8092</identifier><identifier>EISSN: 1096-9101</identifier><identifier>DOI: 10.1002/lsm.22027</identifier><identifier>PMID: 22488690</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenosine Triphosphate - metabolism ; Biomarkers - metabolism ; Cells, Cultured ; diabetes ; Diabetes Mellitus ; electron transport chain ; Electron Transport Chain Complex Proteins - metabolism ; Electron Transport Complex IV - metabolism ; Fibroblasts - metabolism ; Fibroblasts - radiation effects ; Humans ; Ischemia ; Lasers, Semiconductor ; Light ; mitochondria ; Mitochondria - metabolism ; Mitochondria - radiation effects ; phototherapy ; Skin - cytology ; Stress, Physiological - physiology ; Stress, Physiological - radiation effects</subject><ispartof>Lasers in surgery and medicine, 2012-07, Vol.44 (5), p.429-434</ispartof><rights>Copyright © 2012 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3447-1a23a38e4418ffb1be5d492ed5dec6870a43250ed03c0bd560e425d233b4b6513</citedby><cites>FETCH-LOGICAL-c3447-1a23a38e4418ffb1be5d492ed5dec6870a43250ed03c0bd560e425d233b4b6513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Flsm.22027$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Flsm.22027$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22488690$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Houreld, Nicolette N.</creatorcontrib><creatorcontrib>Masha, Roland T.</creatorcontrib><creatorcontrib>Abrahamse, Heidi</creatorcontrib><title>Low-intensity laser irradiation at 660 nm stimulates cytochrome c oxidase in stressed fibroblast cells</title><title>Lasers in surgery and medicine</title><addtitle>Lasers Surg. Med</addtitle><description>Background and Objective
Low‐intensity laser irradiation (LILI) has been used to modulate a variety of biological processes, including diabetic wound healing. The mechanism of action is thought to exist primarily with the mitochondria. This study aimed to determine the effect of irradiation on normal, diabetic, and ischemic mitochondrial electron transport chain (ETC) complexes.
Materials and Methods
Normal, diabetic and ischemic human skin fibroblast mitochondria were irradiated in vitro at a wavelength of 660 nm and a fluence of either 5 or 15 J/cm2. Non‐irradiated mitochondria served as controls. Enzyme activities of mitochondrial complexes I, II, III, and IV were determined immediately post‐irradiation. Normal, diabetic, and ischemic cells were irradiated and adenosine triphosphate (ATP) and active mitochondria were determined by luminescence and fluorescent microscopy, respectively.
Results
Irradiated diabetic mitochondria at a fluence of 15 J/cm2 showed a significant decrease in complex III activity (P < 0.05). Normal (P < 0.01) and diabetic (P < 0.05) mitochondria irradiated at either 5 or 15 J/cm2 showed a significant increase in complex IV activity. ATP results showed a significant increase in irradiated normal cells (5 J/cm2; P < 0.05) and diabetic cells (15 J/cm2; P < 0.01). There was a higher accumulation of active mitochondria in irradiated cells than non‐irradiated cells.
Conclusion
Irradiation at 660 nm has the ability to influence mitochondrial enzyme activity, in particular cytochrome c oxidase. This leads to increased mitochondrial activity and ATP synthesis. Lasers Surg. Med. 44: 429–434, 2012. © 2012 Wiley Periodicals, Inc.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Biomarkers - metabolism</subject><subject>Cells, Cultured</subject><subject>diabetes</subject><subject>Diabetes Mellitus</subject><subject>electron transport chain</subject><subject>Electron Transport Chain Complex Proteins - metabolism</subject><subject>Electron Transport Complex IV - metabolism</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - radiation effects</subject><subject>Humans</subject><subject>Ischemia</subject><subject>Lasers, Semiconductor</subject><subject>Light</subject><subject>mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondria - radiation effects</subject><subject>phototherapy</subject><subject>Skin - cytology</subject><subject>Stress, Physiological - physiology</subject><subject>Stress, Physiological - radiation effects</subject><issn>0196-8092</issn><issn>1096-9101</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtv1DAURi0EokNhwR9AXsIi7fUjTrJEVR9IU5B4qEvLiW9UQxIXX4_a2bHlb_JL8DBtd6x8F-c7sg5jrwUcCQB5PNF8JCXI5glbCehM1QkQT9kKRLlb6OQBe0H0HQCUhOY5O5BSt63pYMWu1_G2CkvGhULe8skRJh5Scj64HOLCXebGwJ9fv5eZUw7zZnIZiQ_bHIfrFGfkA493wZchD0tBEhKh52PoU-yLL_MBp4lesmejmwhf3b-H7NvZ6deTi2r96fzDyft1NSitm0o4qZxqUWvRjmMveqy97iT62uNg2gacVrIG9KAG6H1tALWsvVSq172phTpkb_femxR_bpCynQPtfuAWjBuyYhdFGCHagr7bo0OKRAlHe5PC7NK2QHYX1paw9l_Ywr651276Gf0j-VCyAMd74DZMuP2_ya6_XD4oq_0iUMa7x4VLP6xpVFPbq4_n9vOFvNJnl52V6i8NeJLq</recordid><startdate>201207</startdate><enddate>201207</enddate><creator>Houreld, Nicolette N.</creator><creator>Masha, Roland T.</creator><creator>Abrahamse, Heidi</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201207</creationdate><title>Low-intensity laser irradiation at 660 nm stimulates cytochrome c oxidase in stressed fibroblast cells</title><author>Houreld, Nicolette N. ; Masha, Roland T. ; Abrahamse, Heidi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3447-1a23a38e4418ffb1be5d492ed5dec6870a43250ed03c0bd560e425d233b4b6513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Biomarkers - metabolism</topic><topic>Cells, Cultured</topic><topic>diabetes</topic><topic>Diabetes Mellitus</topic><topic>electron transport chain</topic><topic>Electron Transport Chain Complex Proteins - metabolism</topic><topic>Electron Transport Complex IV - metabolism</topic><topic>Fibroblasts - metabolism</topic><topic>Fibroblasts - radiation effects</topic><topic>Humans</topic><topic>Ischemia</topic><topic>Lasers, Semiconductor</topic><topic>Light</topic><topic>mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondria - radiation effects</topic><topic>phototherapy</topic><topic>Skin - cytology</topic><topic>Stress, Physiological - physiology</topic><topic>Stress, Physiological - radiation effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Houreld, Nicolette N.</creatorcontrib><creatorcontrib>Masha, Roland T.</creatorcontrib><creatorcontrib>Abrahamse, Heidi</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Lasers in surgery and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Houreld, Nicolette N.</au><au>Masha, Roland T.</au><au>Abrahamse, Heidi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low-intensity laser irradiation at 660 nm stimulates cytochrome c oxidase in stressed fibroblast cells</atitle><jtitle>Lasers in surgery and medicine</jtitle><addtitle>Lasers Surg. Med</addtitle><date>2012-07</date><risdate>2012</risdate><volume>44</volume><issue>5</issue><spage>429</spage><epage>434</epage><pages>429-434</pages><issn>0196-8092</issn><eissn>1096-9101</eissn><abstract>Background and Objective
Low‐intensity laser irradiation (LILI) has been used to modulate a variety of biological processes, including diabetic wound healing. The mechanism of action is thought to exist primarily with the mitochondria. This study aimed to determine the effect of irradiation on normal, diabetic, and ischemic mitochondrial electron transport chain (ETC) complexes.
Materials and Methods
Normal, diabetic and ischemic human skin fibroblast mitochondria were irradiated in vitro at a wavelength of 660 nm and a fluence of either 5 or 15 J/cm2. Non‐irradiated mitochondria served as controls. Enzyme activities of mitochondrial complexes I, II, III, and IV were determined immediately post‐irradiation. Normal, diabetic, and ischemic cells were irradiated and adenosine triphosphate (ATP) and active mitochondria were determined by luminescence and fluorescent microscopy, respectively.
Results
Irradiated diabetic mitochondria at a fluence of 15 J/cm2 showed a significant decrease in complex III activity (P < 0.05). Normal (P < 0.01) and diabetic (P < 0.05) mitochondria irradiated at either 5 or 15 J/cm2 showed a significant increase in complex IV activity. ATP results showed a significant increase in irradiated normal cells (5 J/cm2; P < 0.05) and diabetic cells (15 J/cm2; P < 0.01). There was a higher accumulation of active mitochondria in irradiated cells than non‐irradiated cells.
Conclusion
Irradiation at 660 nm has the ability to influence mitochondrial enzyme activity, in particular cytochrome c oxidase. This leads to increased mitochondrial activity and ATP synthesis. Lasers Surg. Med. 44: 429–434, 2012. © 2012 Wiley Periodicals, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>22488690</pmid><doi>10.1002/lsm.22027</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adenosine Triphosphate - metabolism Biomarkers - metabolism Cells, Cultured diabetes Diabetes Mellitus electron transport chain Electron Transport Chain Complex Proteins - metabolism Electron Transport Complex IV - metabolism Fibroblasts - metabolism Fibroblasts - radiation effects Humans Ischemia Lasers, Semiconductor Light mitochondria Mitochondria - metabolism Mitochondria - radiation effects phototherapy Skin - cytology Stress, Physiological - physiology Stress, Physiological - radiation effects |
| title | Low-intensity laser irradiation at 660 nm stimulates cytochrome c oxidase in stressed fibroblast cells |
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