Effect of Relaxin on Human Sperm Functions
Relaxin is a circulating hormone with functions in pregnancy, parturition, and other aspects of female reproduction. It is also secreted from the prostate gland into the seminal fluid; however, the role of relaxin in male reproduction is debated. Studies conducted in the past have suggested possible...
Gespeichert in:
| Veröffentlicht in: | Journal of andrology 2012-05, Vol.33 (3), p.474-482 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 482 |
|---|---|
| container_issue | 3 |
| container_start_page | 474 |
| container_title | Journal of andrology |
| container_volume | 33 |
| creator | Ferlin, Alberto Menegazzo, Massimo Gianesello, Lisa Selice, Riccardo Foresta, Carlo |
| description | Relaxin is a circulating hormone with functions in pregnancy, parturition, and other aspects of female reproduction. It is also secreted from the prostate gland into the seminal fluid; however, the role of relaxin in male reproduction is debated. Studies conducted in the past have suggested possible actions on human spermatozoa, but the data were contrasting. Here, we show that the relaxin receptor RXFP1 (Relaxin Family Peptide Receptor 1) is expressed in human spermatozoa, and it mainly localizes in the astrodome. In vitro studies on human sperm demonstrated that this hormone attenuates the natural decline in sperm motility and maintains higher mitochondrial activity and lower apoptosis level. Furthermore, relaxin induced an increase in sperm hyperactivation, intracellular calcium and cAMP, and acrosome reaction. These effects were abolished by the use of the specific anti‐RXFP1 antibody. Relaxin concentrations were low in the blood (x̄ ± SD, 0.16 ± 0.03 nM) and very high in the seminal plasma (x̄ ± SD, 10.3 ± 4.0 nM), confirming its secretion mainly by the prostate. Taken together, these data demonstrate that relaxin influences positively many sperm functions linked to fertilizing ability, and it preserves sperm functionality, with possible practical value in assisted reproduction techniques. |
| doi_str_mv | 10.2164/jandrol.110.012625 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1017983984</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1017983984</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4489-aeaf12f87aaf5aea8e24cbf535c2c70a49d74fae13a761a006fec1ce742646bf3</originalsourceid><addsrcrecordid>eNqNkEtLAzEQgIMotlb_gAfZiyDC1ryzOZbaWqUo-Dgv0zSBLfuoSRftvzeyq149DTN88_oQOid4TInkNxuo174pxyQWMKGSigM0JJrplEuOD9EQEy1TJpkYoJMQNhhTTBQ7RgNKNGZasSG6njlnzS5pXPJsS_gs6qSpk0VbQZ28bK2vknlbm13R1OEUHTkogz3r4wi9zWev00W6fLq7n06WqeE80ylYcIS6TAE4EZPMUm5WTjBhqFEYuF4r7sASBkoSwFjGA4ixilPJ5cqxEbrq5m59897asMurIhhbllDbpg05iU_ojOmMR5R2qPFNCN66fOuLCvw-Qvm3o7x3lEdHeecoNl3089tVZde_LT9SInDZAxAMlM5DbYrwxwktM0JV5LKO-yhKu__H6vxh8nhLhNDsC-QxgYQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1017983984</pqid></control><display><type>article</type><title>Effect of Relaxin on Human Sperm Functions</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Wiley Free Content</source><creator>Ferlin, Alberto ; Menegazzo, Massimo ; Gianesello, Lisa ; Selice, Riccardo ; Foresta, Carlo</creator><creatorcontrib>Ferlin, Alberto ; Menegazzo, Massimo ; Gianesello, Lisa ; Selice, Riccardo ; Foresta, Carlo</creatorcontrib><description>Relaxin is a circulating hormone with functions in pregnancy, parturition, and other aspects of female reproduction. It is also secreted from the prostate gland into the seminal fluid; however, the role of relaxin in male reproduction is debated. Studies conducted in the past have suggested possible actions on human spermatozoa, but the data were contrasting. Here, we show that the relaxin receptor RXFP1 (Relaxin Family Peptide Receptor 1) is expressed in human spermatozoa, and it mainly localizes in the astrodome. In vitro studies on human sperm demonstrated that this hormone attenuates the natural decline in sperm motility and maintains higher mitochondrial activity and lower apoptosis level. Furthermore, relaxin induced an increase in sperm hyperactivation, intracellular calcium and cAMP, and acrosome reaction. These effects were abolished by the use of the specific anti‐RXFP1 antibody. Relaxin concentrations were low in the blood (x̄ ± SD, 0.16 ± 0.03 nM) and very high in the seminal plasma (x̄ ± SD, 10.3 ± 4.0 nM), confirming its secretion mainly by the prostate. Taken together, these data demonstrate that relaxin influences positively many sperm functions linked to fertilizing ability, and it preserves sperm functionality, with possible practical value in assisted reproduction techniques.</description><identifier>ISSN: 0196-3635</identifier><identifier>EISSN: 1939-4640</identifier><identifier>DOI: 10.2164/jandrol.110.012625</identifier><identifier>PMID: 21903973</identifier><identifier>CODEN: JOAND3</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acrosome reaction ; Acrosome Reaction - physiology ; Apoptosis - physiology ; Asthenozoospermia - physiopathology ; Biological and medical sciences ; Calcium - analysis ; capacitation ; Cyclic AMP - analysis ; Fertilization - physiology ; Fundamental and applied biological sciences. Psychology ; Gynecology. Andrology. Obstetrics ; Humans ; Male ; Male genital diseases ; Mammalian male genital system ; Medical sciences ; Mitochondria - metabolism ; Prostate - secretion ; Receptors, G-Protein-Coupled - analysis ; Receptors, G-Protein-Coupled - metabolism ; Receptors, Peptide - analysis ; Receptors, Peptide - metabolism ; Relaxin - blood ; Relaxin - pharmacology ; Relaxin - physiology ; RXFP1 ; Semen Analysis ; sperm apoptosis ; Sperm Capacitation - physiology ; sperm motility ; Spermatozoa - drug effects ; Spermatozoa - metabolism ; Spermatozoa - physiology ; Vertebrates: reproduction</subject><ispartof>Journal of andrology, 2012-05, Vol.33 (3), p.474-482</ispartof><rights>2012 American Society of Andrology</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4489-aeaf12f87aaf5aea8e24cbf535c2c70a49d74fae13a761a006fec1ce742646bf3</citedby><cites>FETCH-LOGICAL-c4489-aeaf12f87aaf5aea8e24cbf535c2c70a49d74fae13a761a006fec1ce742646bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.2164%2Fjandrol.110.012625$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.2164%2Fjandrol.110.012625$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1433,27924,27925,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25968127$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21903973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferlin, Alberto</creatorcontrib><creatorcontrib>Menegazzo, Massimo</creatorcontrib><creatorcontrib>Gianesello, Lisa</creatorcontrib><creatorcontrib>Selice, Riccardo</creatorcontrib><creatorcontrib>Foresta, Carlo</creatorcontrib><title>Effect of Relaxin on Human Sperm Functions</title><title>Journal of andrology</title><addtitle>J Androl</addtitle><description>Relaxin is a circulating hormone with functions in pregnancy, parturition, and other aspects of female reproduction. It is also secreted from the prostate gland into the seminal fluid; however, the role of relaxin in male reproduction is debated. Studies conducted in the past have suggested possible actions on human spermatozoa, but the data were contrasting. Here, we show that the relaxin receptor RXFP1 (Relaxin Family Peptide Receptor 1) is expressed in human spermatozoa, and it mainly localizes in the astrodome. In vitro studies on human sperm demonstrated that this hormone attenuates the natural decline in sperm motility and maintains higher mitochondrial activity and lower apoptosis level. Furthermore, relaxin induced an increase in sperm hyperactivation, intracellular calcium and cAMP, and acrosome reaction. These effects were abolished by the use of the specific anti‐RXFP1 antibody. Relaxin concentrations were low in the blood (x̄ ± SD, 0.16 ± 0.03 nM) and very high in the seminal plasma (x̄ ± SD, 10.3 ± 4.0 nM), confirming its secretion mainly by the prostate. Taken together, these data demonstrate that relaxin influences positively many sperm functions linked to fertilizing ability, and it preserves sperm functionality, with possible practical value in assisted reproduction techniques.</description><subject>Acrosome reaction</subject><subject>Acrosome Reaction - physiology</subject><subject>Apoptosis - physiology</subject><subject>Asthenozoospermia - physiopathology</subject><subject>Biological and medical sciences</subject><subject>Calcium - analysis</subject><subject>capacitation</subject><subject>Cyclic AMP - analysis</subject><subject>Fertilization - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Male</subject><subject>Male genital diseases</subject><subject>Mammalian male genital system</subject><subject>Medical sciences</subject><subject>Mitochondria - metabolism</subject><subject>Prostate - secretion</subject><subject>Receptors, G-Protein-Coupled - analysis</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Receptors, Peptide - analysis</subject><subject>Receptors, Peptide - metabolism</subject><subject>Relaxin - blood</subject><subject>Relaxin - pharmacology</subject><subject>Relaxin - physiology</subject><subject>RXFP1</subject><subject>Semen Analysis</subject><subject>sperm apoptosis</subject><subject>Sperm Capacitation - physiology</subject><subject>sperm motility</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - metabolism</subject><subject>Spermatozoa - physiology</subject><subject>Vertebrates: reproduction</subject><issn>0196-3635</issn><issn>1939-4640</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtLAzEQgIMotlb_gAfZiyDC1ryzOZbaWqUo-Dgv0zSBLfuoSRftvzeyq149DTN88_oQOid4TInkNxuo174pxyQWMKGSigM0JJrplEuOD9EQEy1TJpkYoJMQNhhTTBQ7RgNKNGZasSG6njlnzS5pXPJsS_gs6qSpk0VbQZ28bK2vknlbm13R1OEUHTkogz3r4wi9zWev00W6fLq7n06WqeE80ylYcIS6TAE4EZPMUm5WTjBhqFEYuF4r7sASBkoSwFjGA4ixilPJ5cqxEbrq5m59897asMurIhhbllDbpg05iU_ojOmMR5R2qPFNCN66fOuLCvw-Qvm3o7x3lEdHeecoNl3089tVZde_LT9SInDZAxAMlM5DbYrwxwktM0JV5LKO-yhKu__H6vxh8nhLhNDsC-QxgYQ</recordid><startdate>201205</startdate><enddate>201205</enddate><creator>Ferlin, Alberto</creator><creator>Menegazzo, Massimo</creator><creator>Gianesello, Lisa</creator><creator>Selice, Riccardo</creator><creator>Foresta, Carlo</creator><general>Blackwell Publishing Ltd</general><general>American Society of Andrology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201205</creationdate><title>Effect of Relaxin on Human Sperm Functions</title><author>Ferlin, Alberto ; Menegazzo, Massimo ; Gianesello, Lisa ; Selice, Riccardo ; Foresta, Carlo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4489-aeaf12f87aaf5aea8e24cbf535c2c70a49d74fae13a761a006fec1ce742646bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acrosome reaction</topic><topic>Acrosome Reaction - physiology</topic><topic>Apoptosis - physiology</topic><topic>Asthenozoospermia - physiopathology</topic><topic>Biological and medical sciences</topic><topic>Calcium - analysis</topic><topic>capacitation</topic><topic>Cyclic AMP - analysis</topic><topic>Fertilization - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Male</topic><topic>Male genital diseases</topic><topic>Mammalian male genital system</topic><topic>Medical sciences</topic><topic>Mitochondria - metabolism</topic><topic>Prostate - secretion</topic><topic>Receptors, G-Protein-Coupled - analysis</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Receptors, Peptide - analysis</topic><topic>Receptors, Peptide - metabolism</topic><topic>Relaxin - blood</topic><topic>Relaxin - pharmacology</topic><topic>Relaxin - physiology</topic><topic>RXFP1</topic><topic>Semen Analysis</topic><topic>sperm apoptosis</topic><topic>Sperm Capacitation - physiology</topic><topic>sperm motility</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - metabolism</topic><topic>Spermatozoa - physiology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferlin, Alberto</creatorcontrib><creatorcontrib>Menegazzo, Massimo</creatorcontrib><creatorcontrib>Gianesello, Lisa</creatorcontrib><creatorcontrib>Selice, Riccardo</creatorcontrib><creatorcontrib>Foresta, Carlo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of andrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferlin, Alberto</au><au>Menegazzo, Massimo</au><au>Gianesello, Lisa</au><au>Selice, Riccardo</au><au>Foresta, Carlo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Relaxin on Human Sperm Functions</atitle><jtitle>Journal of andrology</jtitle><addtitle>J Androl</addtitle><date>2012-05</date><risdate>2012</risdate><volume>33</volume><issue>3</issue><spage>474</spage><epage>482</epage><pages>474-482</pages><issn>0196-3635</issn><eissn>1939-4640</eissn><coden>JOAND3</coden><abstract>Relaxin is a circulating hormone with functions in pregnancy, parturition, and other aspects of female reproduction. It is also secreted from the prostate gland into the seminal fluid; however, the role of relaxin in male reproduction is debated. Studies conducted in the past have suggested possible actions on human spermatozoa, but the data were contrasting. Here, we show that the relaxin receptor RXFP1 (Relaxin Family Peptide Receptor 1) is expressed in human spermatozoa, and it mainly localizes in the astrodome. In vitro studies on human sperm demonstrated that this hormone attenuates the natural decline in sperm motility and maintains higher mitochondrial activity and lower apoptosis level. Furthermore, relaxin induced an increase in sperm hyperactivation, intracellular calcium and cAMP, and acrosome reaction. These effects were abolished by the use of the specific anti‐RXFP1 antibody. Relaxin concentrations were low in the blood (x̄ ± SD, 0.16 ± 0.03 nM) and very high in the seminal plasma (x̄ ± SD, 10.3 ± 4.0 nM), confirming its secretion mainly by the prostate. Taken together, these data demonstrate that relaxin influences positively many sperm functions linked to fertilizing ability, and it preserves sperm functionality, with possible practical value in assisted reproduction techniques.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21903973</pmid><doi>10.2164/jandrol.110.012625</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0196-3635 |
| ispartof | Journal of andrology, 2012-05, Vol.33 (3), p.474-482 |
| issn | 0196-3635 1939-4640 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1017983984 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content |
| subjects | Acrosome reaction Acrosome Reaction - physiology Apoptosis - physiology Asthenozoospermia - physiopathology Biological and medical sciences Calcium - analysis capacitation Cyclic AMP - analysis Fertilization - physiology Fundamental and applied biological sciences. Psychology Gynecology. Andrology. Obstetrics Humans Male Male genital diseases Mammalian male genital system Medical sciences Mitochondria - metabolism Prostate - secretion Receptors, G-Protein-Coupled - analysis Receptors, G-Protein-Coupled - metabolism Receptors, Peptide - analysis Receptors, Peptide - metabolism Relaxin - blood Relaxin - pharmacology Relaxin - physiology RXFP1 Semen Analysis sperm apoptosis Sperm Capacitation - physiology sperm motility Spermatozoa - drug effects Spermatozoa - metabolism Spermatozoa - physiology Vertebrates: reproduction |
| title | Effect of Relaxin on Human Sperm Functions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T15%3A30%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20Relaxin%20on%20Human%20Sperm%20Functions&rft.jtitle=Journal%20of%20andrology&rft.au=Ferlin,%20Alberto&rft.date=2012-05&rft.volume=33&rft.issue=3&rft.spage=474&rft.epage=482&rft.pages=474-482&rft.issn=0196-3635&rft.eissn=1939-4640&rft.coden=JOAND3&rft_id=info:doi/10.2164/jandrol.110.012625&rft_dat=%3Cproquest_cross%3E1017983984%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1017983984&rft_id=info:pmid/21903973&rfr_iscdi=true |