Structural changes in freshwater fish and chironomids exposed to bacterial exotoxins
Mass fish mortalities have been reported in the past decade from British waters, often coinciding with blooms of filamentous actinobacteria, particularly strains of Streptomyces griseus. The present study has shown that some fractions of the exudate of S. griseus, prepared after a series of high pre...
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| Veröffentlicht in: | Ecotoxicology and environmental safety 2012-06, Vol.80, p.37-44 |
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| creator | Lewis, J.W. Morley, N.J. Ahmad, M. Challis, G.L. Wright, R. Bicker, R. Morritt, D. |
| description | Mass fish mortalities have been reported in the past decade from British waters, often coinciding with blooms of filamentous actinobacteria, particularly strains of Streptomyces griseus. The present study has shown that some fractions of the exudate of S. griseus, prepared after a series of high pressure liquid chromatography (HPLC) separations, and analysed with liquid chromatography mass spectrometry (LC–MS), induced pathological changes to the gills of carp and/or tench fry following exposure under laboratory conditions up to 96h Similar changes were induced by streptomycin, a secondary metabolite of S. griseus, and these included loss of microridging and fusion of secondary lamellae, with carp fry in the case of the exudate of S. griseus being more sensitive than tench fry, especially with exposure to fraction 9 and selected sub-fractions of 9. Some deformities using a severity index were also observed in the head capsule of larvae of the non-biting midge Chironomus riparius, including loss and splitting of teeth on the mentum. The results are discussed in relation to further identification of metabolites derived from samples of the organic filtrate of S. griseus and implications for the functioning of freshwater ecosystems.
► Mass freshwater fish mortalities have increasingly occurred in the UK. ► These events coincide with blooms of filamentous actinobacteria. ► Streptomyces griseus is the most frequently occurring actinobacterium. ► Metabolite fractions of S. griseus cause deformities to fish gills and chironimids. ► Fraction identification allows the development of operational tools for managing events. |
| doi_str_mv | 10.1016/j.ecoenv.2012.02.006 |
| format | Article |
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► Mass freshwater fish mortalities have increasingly occurred in the UK. ► These events coincide with blooms of filamentous actinobacteria. ► Streptomyces griseus is the most frequently occurring actinobacterium. ► Metabolite fractions of S. griseus cause deformities to fish gills and chironimids. ► Fraction identification allows the development of operational tools for managing events.</description><identifier>ISSN: 0147-6513</identifier><identifier>EISSN: 1090-2414</identifier><identifier>DOI: 10.1016/j.ecoenv.2012.02.006</identifier><identifier>PMID: 22381615</identifier><identifier>CODEN: EESADV</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Actinobacteria ; Agnatha. Pisces ; Animal, plant and microbial ecology ; Animals ; Applied ecology ; Bacteria ; Biological and medical sciences ; Carp ; Carps ; Chironomus riparius ; Chromatography, High Pressure Liquid ; ecosystems ; Ecotoxicology, biological effects of pollution ; Exotoxin ; exotoxins ; Exotoxins - toxicity ; Exudation ; filtrates ; Fish ; Fresh Water - chemistry ; Freshwater ; freshwater fish ; Freshwaters ; Fundamental and applied biological sciences. Psychology ; General aspects ; gills ; Gills - drug effects ; Gills - pathology ; Gills - ultrastructure ; head ; high performance liquid chromatography ; Insect ; insect larvae ; Larva ; Liquid chromatography ; Mass Spectrometry ; Metabolites ; mortality ; Pathology ; secondary metabolites ; Streptomyces ; Streptomyces griseus ; Streptomyces griseus - metabolism ; streptomycin ; Teeth ; Tinca tinca ; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution ; Water Pollutants, Chemical - toxicity</subject><ispartof>Ecotoxicology and environmental safety, 2012-06, Vol.80, p.37-44</ispartof><rights>2012 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-c1b43060d1c11d478ee91f929e7f4ea41eaa51499e4b43aec497de023988e1603</citedby><cites>FETCH-LOGICAL-c449t-c1b43060d1c11d478ee91f929e7f4ea41eaa51499e4b43aec497de023988e1603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0147651312000309$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25905933$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22381615$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lewis, J.W.</creatorcontrib><creatorcontrib>Morley, N.J.</creatorcontrib><creatorcontrib>Ahmad, M.</creatorcontrib><creatorcontrib>Challis, G.L.</creatorcontrib><creatorcontrib>Wright, R.</creatorcontrib><creatorcontrib>Bicker, R.</creatorcontrib><creatorcontrib>Morritt, D.</creatorcontrib><title>Structural changes in freshwater fish and chironomids exposed to bacterial exotoxins</title><title>Ecotoxicology and environmental safety</title><addtitle>Ecotoxicol Environ Saf</addtitle><description>Mass fish mortalities have been reported in the past decade from British waters, often coinciding with blooms of filamentous actinobacteria, particularly strains of Streptomyces griseus. The present study has shown that some fractions of the exudate of S. griseus, prepared after a series of high pressure liquid chromatography (HPLC) separations, and analysed with liquid chromatography mass spectrometry (LC–MS), induced pathological changes to the gills of carp and/or tench fry following exposure under laboratory conditions up to 96h Similar changes were induced by streptomycin, a secondary metabolite of S. griseus, and these included loss of microridging and fusion of secondary lamellae, with carp fry in the case of the exudate of S. griseus being more sensitive than tench fry, especially with exposure to fraction 9 and selected sub-fractions of 9. Some deformities using a severity index were also observed in the head capsule of larvae of the non-biting midge Chironomus riparius, including loss and splitting of teeth on the mentum. The results are discussed in relation to further identification of metabolites derived from samples of the organic filtrate of S. griseus and implications for the functioning of freshwater ecosystems.
► Mass freshwater fish mortalities have increasingly occurred in the UK. ► These events coincide with blooms of filamentous actinobacteria. ► Streptomyces griseus is the most frequently occurring actinobacterium. ► Metabolite fractions of S. griseus cause deformities to fish gills and chironimids. ► Fraction identification allows the development of operational tools for managing events.</description><subject>Actinobacteria</subject><subject>Agnatha. Pisces</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Carp</subject><subject>Carps</subject><subject>Chironomus riparius</subject><subject>Chromatography, High Pressure Liquid</subject><subject>ecosystems</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Exotoxin</subject><subject>exotoxins</subject><subject>Exotoxins - toxicity</subject><subject>Exudation</subject><subject>filtrates</subject><subject>Fish</subject><subject>Fresh Water - chemistry</subject><subject>Freshwater</subject><subject>freshwater fish</subject><subject>Freshwaters</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>gills</subject><subject>Gills - drug effects</subject><subject>Gills - pathology</subject><subject>Gills - ultrastructure</subject><subject>head</subject><subject>high performance liquid chromatography</subject><subject>Insect</subject><subject>insect larvae</subject><subject>Larva</subject><subject>Liquid chromatography</subject><subject>Mass Spectrometry</subject><subject>Metabolites</subject><subject>mortality</subject><subject>Pathology</subject><subject>secondary metabolites</subject><subject>Streptomyces</subject><subject>Streptomyces griseus</subject><subject>Streptomyces griseus - metabolism</subject><subject>streptomycin</subject><subject>Teeth</subject><subject>Tinca tinca</subject><subject>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</subject><subject>Water Pollutants, Chemical - toxicity</subject><issn>0147-6513</issn><issn>1090-2414</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0E2LFDEQBuAgijuu_gPRvgheeqzqTtKdiyCLX7DgYXfPIZNU72ToScakex3_vRl61JsIBTnkqQ9exl4irBFQvtutyUYKD-sGsFlDKZCP2ApBQd1w5I_ZCpB3tRTYXrBnOe8AoAUhnrKLpml7lChW7PZmSrOd5mTGym5NuKdc-VANifL2h5koVYPP28oEV759iiHuvcsVHQ8xk6umWG2MLcyXfjrGKR59yM_Zk8GMmV6c30t29-nj7dWX-vrb569XH65ry7maaosb3oIEhxbR8a4nUjioRlE3cDIcyRiBXCniBRqyXHWOoGlV3xNKaC_Z22XuIcXvM-VJ7322NI4mUJyzLjl1qusa0f8PRVEGS1koX6hNMedEgz4kvzfpZ0EnJ_VOL9HrU_QaSsGp7dV5w7zZk_vT9DvrAt6cgcnWjEMywfr81wkFQrVtca8XN5iozX0q5u6mbOIAqKDvTuL9IqiE--Ap6Ww9BUvOJ7KTdtH_-9ZfPwasuA</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>Lewis, J.W.</creator><creator>Morley, N.J.</creator><creator>Ahmad, M.</creator><creator>Challis, G.L.</creator><creator>Wright, R.</creator><creator>Bicker, R.</creator><creator>Morritt, D.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SU</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20120601</creationdate><title>Structural changes in freshwater fish and chironomids exposed to bacterial exotoxins</title><author>Lewis, J.W. ; Morley, N.J. ; Ahmad, M. ; Challis, G.L. ; Wright, R. ; Bicker, R. ; Morritt, D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-c1b43060d1c11d478ee91f929e7f4ea41eaa51499e4b43aec497de023988e1603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Actinobacteria</topic><topic>Agnatha. Pisces</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Carp</topic><topic>Carps</topic><topic>Chironomus riparius</topic><topic>Chromatography, High Pressure Liquid</topic><topic>ecosystems</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Exotoxin</topic><topic>exotoxins</topic><topic>Exotoxins - toxicity</topic><topic>Exudation</topic><topic>filtrates</topic><topic>Fish</topic><topic>Fresh Water - chemistry</topic><topic>Freshwater</topic><topic>freshwater fish</topic><topic>Freshwaters</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>gills</topic><topic>Gills - drug effects</topic><topic>Gills - pathology</topic><topic>Gills - ultrastructure</topic><topic>head</topic><topic>high performance liquid chromatography</topic><topic>Insect</topic><topic>insect larvae</topic><topic>Larva</topic><topic>Liquid chromatography</topic><topic>Mass Spectrometry</topic><topic>Metabolites</topic><topic>mortality</topic><topic>Pathology</topic><topic>secondary metabolites</topic><topic>Streptomyces</topic><topic>Streptomyces griseus</topic><topic>Streptomyces griseus - metabolism</topic><topic>streptomycin</topic><topic>Teeth</topic><topic>Tinca tinca</topic><topic>Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution</topic><topic>Water Pollutants, Chemical - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lewis, J.W.</creatorcontrib><creatorcontrib>Morley, N.J.</creatorcontrib><creatorcontrib>Ahmad, M.</creatorcontrib><creatorcontrib>Challis, G.L.</creatorcontrib><creatorcontrib>Wright, R.</creatorcontrib><creatorcontrib>Bicker, R.</creatorcontrib><creatorcontrib>Morritt, D.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environmental Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Ecotoxicology and environmental safety</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lewis, J.W.</au><au>Morley, N.J.</au><au>Ahmad, M.</au><au>Challis, G.L.</au><au>Wright, R.</au><au>Bicker, R.</au><au>Morritt, D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural changes in freshwater fish and chironomids exposed to bacterial exotoxins</atitle><jtitle>Ecotoxicology and environmental safety</jtitle><addtitle>Ecotoxicol Environ Saf</addtitle><date>2012-06-01</date><risdate>2012</risdate><volume>80</volume><spage>37</spage><epage>44</epage><pages>37-44</pages><issn>0147-6513</issn><eissn>1090-2414</eissn><coden>EESADV</coden><abstract>Mass fish mortalities have been reported in the past decade from British waters, often coinciding with blooms of filamentous actinobacteria, particularly strains of Streptomyces griseus. The present study has shown that some fractions of the exudate of S. griseus, prepared after a series of high pressure liquid chromatography (HPLC) separations, and analysed with liquid chromatography mass spectrometry (LC–MS), induced pathological changes to the gills of carp and/or tench fry following exposure under laboratory conditions up to 96h Similar changes were induced by streptomycin, a secondary metabolite of S. griseus, and these included loss of microridging and fusion of secondary lamellae, with carp fry in the case of the exudate of S. griseus being more sensitive than tench fry, especially with exposure to fraction 9 and selected sub-fractions of 9. Some deformities using a severity index were also observed in the head capsule of larvae of the non-biting midge Chironomus riparius, including loss and splitting of teeth on the mentum. The results are discussed in relation to further identification of metabolites derived from samples of the organic filtrate of S. griseus and implications for the functioning of freshwater ecosystems.
► Mass freshwater fish mortalities have increasingly occurred in the UK. ► These events coincide with blooms of filamentous actinobacteria. ► Streptomyces griseus is the most frequently occurring actinobacterium. ► Metabolite fractions of S. griseus cause deformities to fish gills and chironimids. ► Fraction identification allows the development of operational tools for managing events.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>22381615</pmid><doi>10.1016/j.ecoenv.2012.02.006</doi><tpages>8</tpages></addata></record> |
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| subjects | Actinobacteria Agnatha. Pisces Animal, plant and microbial ecology Animals Applied ecology Bacteria Biological and medical sciences Carp Carps Chironomus riparius Chromatography, High Pressure Liquid ecosystems Ecotoxicology, biological effects of pollution Exotoxin exotoxins Exotoxins - toxicity Exudation filtrates Fish Fresh Water - chemistry Freshwater freshwater fish Freshwaters Fundamental and applied biological sciences. Psychology General aspects gills Gills - drug effects Gills - pathology Gills - ultrastructure head high performance liquid chromatography Insect insect larvae Larva Liquid chromatography Mass Spectrometry Metabolites mortality Pathology secondary metabolites Streptomyces Streptomyces griseus Streptomyces griseus - metabolism streptomycin Teeth Tinca tinca Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution Water Pollutants, Chemical - toxicity |
| title | Structural changes in freshwater fish and chironomids exposed to bacterial exotoxins |
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