Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia
Ehrlichiosis and babesiosis are tick-borne diseases, caused mainly by Ehrlichia canis and Babesia canis, respectively, with a worldwide occurrence in dogs, whose main vector is the brown-dog tick, Rhipicephalus sanguineus. The present work aimed to detect the presence of E. canis and Babesia sp. in...
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| Veröffentlicht in: | Veterinary parasitology 2012-05, Vol.186 (3-4), p.254-260 |
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| creator | Vargas-Hernández, G. André, M.R. Faria, J.L.M. Munhoz, T.D. Hernandez-Rodriguez, M. Machado, R.Z. Tinucci-Costa, M. |
| description | Ehrlichiosis and babesiosis are tick-borne diseases, caused mainly by Ehrlichia canis and Babesia canis, respectively, with a worldwide occurrence in dogs, whose main vector is the brown-dog tick, Rhipicephalus sanguineus. The present work aimed to detect the presence of E. canis and Babesia sp. in 91 dog blood samples in Colombia, by molecular and serological techniques. We also performed sequence alignment to indicate the identity of the parasite species infecting these animals. The present work shows the first molecular detection of E. canis and B. vogeli in dogs from Colombia. Immunoglobulin-G (IgG) antibodies to E. canis and Babesia vogeli were found in 75 (82.4%) and 47 (51.6%) sampled dogs, respectively. Thirty-seven (40.6%) and 5 (5.5%) dogs were positive in PCR for E. canis and Babesia sp., respectively. After sequencing, amplicons showed 99% of identity with isolates of E. canis and B. vogeli. The phylogenetic trees based on 16S rRNA-Anaplasmataceae sequences and 18S rRNA-piroplasmid sequences supported the identity of the found E. canis and B. vogeli DNAs, respectively. The present work shows the first molecular detection of E. canis and B. vogeli in dogs in Colombia. |
| doi_str_mv | 10.1016/j.vetpar.2011.11.011 |
| format | Article |
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The present work aimed to detect the presence of E. canis and Babesia sp. in 91 dog blood samples in Colombia, by molecular and serological techniques. We also performed sequence alignment to indicate the identity of the parasite species infecting these animals. The present work shows the first molecular detection of E. canis and B. vogeli in dogs from Colombia. Immunoglobulin-G (IgG) antibodies to E. canis and Babesia vogeli were found in 75 (82.4%) and 47 (51.6%) sampled dogs, respectively. Thirty-seven (40.6%) and 5 (5.5%) dogs were positive in PCR for E. canis and Babesia sp., respectively. After sequencing, amplicons showed 99% of identity with isolates of E. canis and B. vogeli. The phylogenetic trees based on 16S rRNA-Anaplasmataceae sequences and 18S rRNA-piroplasmid sequences supported the identity of the found E. canis and B. vogeli DNAs, respectively. The present work shows the first molecular detection of E. canis and B. vogeli in dogs in Colombia.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/j.vetpar.2011.11.011</identifier><identifier>PMID: 22130333</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; antibodies ; Babesia - genetics ; Babesia - isolation & purification ; Babesia canis ; Babesia vogeli ; babesiosis ; Babesiosis - blood ; Babesiosis - epidemiology ; Babesiosis - veterinary ; blood sampling ; Colombia ; Colombia - epidemiology ; Dog Diseases - blood ; Dog Diseases - diagnosis ; Dog Diseases - epidemiology ; Dogs ; Ehrlichia canis ; Ehrlichia canis - genetics ; Ehrlichia canis - isolation & purification ; ehrlichiosis ; Ehrlichiosis - blood ; Ehrlichiosis - epidemiology ; Ehrlichiosis - veterinary ; immunoglobulin G ; Ixodidae ; Phylogeny ; polymerase chain reaction ; Rhipicephalus sanguineus ; sequence alignment ; Seroepidemiologic Studies ; ticks</subject><ispartof>Veterinary parasitology, 2012-05, Vol.186 (3-4), p.254-260</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-392b448179e38f55ad3f07e0b8076ab863412bce4bb499bcebfb73150b68314a3</citedby><cites>FETCH-LOGICAL-c419t-392b448179e38f55ad3f07e0b8076ab863412bce4bb499bcebfb73150b68314a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetpar.2011.11.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22130333$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vargas-Hernández, G.</creatorcontrib><creatorcontrib>André, M.R.</creatorcontrib><creatorcontrib>Faria, J.L.M.</creatorcontrib><creatorcontrib>Munhoz, T.D.</creatorcontrib><creatorcontrib>Hernandez-Rodriguez, M.</creatorcontrib><creatorcontrib>Machado, R.Z.</creatorcontrib><creatorcontrib>Tinucci-Costa, M.</creatorcontrib><title>Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>Ehrlichiosis and babesiosis are tick-borne diseases, caused mainly by Ehrlichia canis and Babesia canis, respectively, with a worldwide occurrence in dogs, whose main vector is the brown-dog tick, Rhipicephalus sanguineus. The present work aimed to detect the presence of E. canis and Babesia sp. in 91 dog blood samples in Colombia, by molecular and serological techniques. We also performed sequence alignment to indicate the identity of the parasite species infecting these animals. The present work shows the first molecular detection of E. canis and B. vogeli in dogs from Colombia. Immunoglobulin-G (IgG) antibodies to E. canis and Babesia vogeli were found in 75 (82.4%) and 47 (51.6%) sampled dogs, respectively. Thirty-seven (40.6%) and 5 (5.5%) dogs were positive in PCR for E. canis and Babesia sp., respectively. After sequencing, amplicons showed 99% of identity with isolates of E. canis and B. vogeli. The phylogenetic trees based on 16S rRNA-Anaplasmataceae sequences and 18S rRNA-piroplasmid sequences supported the identity of the found E. canis and B. vogeli DNAs, respectively. The present work shows the first molecular detection of E. canis and B. vogeli in dogs in Colombia.</description><subject>Animals</subject><subject>antibodies</subject><subject>Babesia - genetics</subject><subject>Babesia - isolation & purification</subject><subject>Babesia canis</subject><subject>Babesia vogeli</subject><subject>babesiosis</subject><subject>Babesiosis - blood</subject><subject>Babesiosis - epidemiology</subject><subject>Babesiosis - veterinary</subject><subject>blood sampling</subject><subject>Colombia</subject><subject>Colombia - epidemiology</subject><subject>Dog Diseases - blood</subject><subject>Dog Diseases - diagnosis</subject><subject>Dog Diseases - epidemiology</subject><subject>Dogs</subject><subject>Ehrlichia canis</subject><subject>Ehrlichia canis - genetics</subject><subject>Ehrlichia canis - isolation & purification</subject><subject>ehrlichiosis</subject><subject>Ehrlichiosis - blood</subject><subject>Ehrlichiosis - epidemiology</subject><subject>Ehrlichiosis - veterinary</subject><subject>immunoglobulin G</subject><subject>Ixodidae</subject><subject>Phylogeny</subject><subject>polymerase chain reaction</subject><subject>Rhipicephalus sanguineus</subject><subject>sequence alignment</subject><subject>Seroepidemiologic Studies</subject><subject>ticks</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFr3DAQhUVpSDbb_IOS-NiLNyNLtuxLoV3SpJCQQ5pjEJI83tWitbaSdyH_vnK86bEUBp4Q37wZ5hHymcKCAq2uN4sDDjsVFgVQukiV5AOZ0VqwvChL-EhmwIDnHKg4I-cxbgCAQyVOyVlRUAaMsRl5efAOzd6pkKm-zSIG7_zKGuWyFgc0g_V95rvsZh2cNWurMqN6G9_g70pjTD8Hv0JnM9tnrV_FUZfJZKut-kROOuUiXhx1Tp5_3Pxa3uX3j7c_l9_uc8NpM-SsKTTnNRUNsrorS9WyDgSCrkFUStcV47TQBrnWvGnSQ3daMFqCrmpGuWJz8mXy3QX_e49xkFsbDTqnevT7KNPBRCMopfw_UGjKggs2onxCTfAxBuzkLtitCq8JGi0ruZFTBnLMQKZKktoujxP2eovt36b3oyfgagI65aVaBRvl81Ny4JDWTJNHi68TgeloB4tBRmOxN9jakDKRrbf_3uEPKeuhwA</recordid><startdate>20120525</startdate><enddate>20120525</enddate><creator>Vargas-Hernández, G.</creator><creator>André, M.R.</creator><creator>Faria, J.L.M.</creator><creator>Munhoz, T.D.</creator><creator>Hernandez-Rodriguez, M.</creator><creator>Machado, R.Z.</creator><creator>Tinucci-Costa, M.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7SS</scope><scope>C1K</scope><scope>M7N</scope></search><sort><creationdate>20120525</creationdate><title>Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia</title><author>Vargas-Hernández, G. ; André, M.R. ; Faria, J.L.M. ; Munhoz, T.D. ; Hernandez-Rodriguez, M. ; Machado, R.Z. ; Tinucci-Costa, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-392b448179e38f55ad3f07e0b8076ab863412bce4bb499bcebfb73150b68314a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>antibodies</topic><topic>Babesia - genetics</topic><topic>Babesia - isolation & purification</topic><topic>Babesia canis</topic><topic>Babesia vogeli</topic><topic>babesiosis</topic><topic>Babesiosis - blood</topic><topic>Babesiosis - epidemiology</topic><topic>Babesiosis - veterinary</topic><topic>blood sampling</topic><topic>Colombia</topic><topic>Colombia - epidemiology</topic><topic>Dog Diseases - blood</topic><topic>Dog Diseases - diagnosis</topic><topic>Dog Diseases - epidemiology</topic><topic>Dogs</topic><topic>Ehrlichia canis</topic><topic>Ehrlichia canis - genetics</topic><topic>Ehrlichia canis - isolation & purification</topic><topic>ehrlichiosis</topic><topic>Ehrlichiosis - blood</topic><topic>Ehrlichiosis - epidemiology</topic><topic>Ehrlichiosis - veterinary</topic><topic>immunoglobulin G</topic><topic>Ixodidae</topic><topic>Phylogeny</topic><topic>polymerase chain reaction</topic><topic>Rhipicephalus sanguineus</topic><topic>sequence alignment</topic><topic>Seroepidemiologic Studies</topic><topic>ticks</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vargas-Hernández, G.</creatorcontrib><creatorcontrib>André, M.R.</creatorcontrib><creatorcontrib>Faria, J.L.M.</creatorcontrib><creatorcontrib>Munhoz, T.D.</creatorcontrib><creatorcontrib>Hernandez-Rodriguez, M.</creatorcontrib><creatorcontrib>Machado, R.Z.</creatorcontrib><creatorcontrib>Tinucci-Costa, M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vargas-Hernández, G.</au><au>André, M.R.</au><au>Faria, J.L.M.</au><au>Munhoz, T.D.</au><au>Hernandez-Rodriguez, M.</au><au>Machado, R.Z.</au><au>Tinucci-Costa, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>2012-05-25</date><risdate>2012</risdate><volume>186</volume><issue>3-4</issue><spage>254</spage><epage>260</epage><pages>254-260</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>Ehrlichiosis and babesiosis are tick-borne diseases, caused mainly by Ehrlichia canis and Babesia canis, respectively, with a worldwide occurrence in dogs, whose main vector is the brown-dog tick, Rhipicephalus sanguineus. The present work aimed to detect the presence of E. canis and Babesia sp. in 91 dog blood samples in Colombia, by molecular and serological techniques. We also performed sequence alignment to indicate the identity of the parasite species infecting these animals. The present work shows the first molecular detection of E. canis and B. vogeli in dogs from Colombia. Immunoglobulin-G (IgG) antibodies to E. canis and Babesia vogeli were found in 75 (82.4%) and 47 (51.6%) sampled dogs, respectively. Thirty-seven (40.6%) and 5 (5.5%) dogs were positive in PCR for E. canis and Babesia sp., respectively. After sequencing, amplicons showed 99% of identity with isolates of E. canis and B. vogeli. The phylogenetic trees based on 16S rRNA-Anaplasmataceae sequences and 18S rRNA-piroplasmid sequences supported the identity of the found E. canis and B. vogeli DNAs, respectively. The present work shows the first molecular detection of E. canis and B. vogeli in dogs in Colombia.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22130333</pmid><doi>10.1016/j.vetpar.2011.11.011</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals antibodies Babesia - genetics Babesia - isolation & purification Babesia canis Babesia vogeli babesiosis Babesiosis - blood Babesiosis - epidemiology Babesiosis - veterinary blood sampling Colombia Colombia - epidemiology Dog Diseases - blood Dog Diseases - diagnosis Dog Diseases - epidemiology Dogs Ehrlichia canis Ehrlichia canis - genetics Ehrlichia canis - isolation & purification ehrlichiosis Ehrlichiosis - blood Ehrlichiosis - epidemiology Ehrlichiosis - veterinary immunoglobulin G Ixodidae Phylogeny polymerase chain reaction Rhipicephalus sanguineus sequence alignment Seroepidemiologic Studies ticks |
| title | Molecular and serological detection of Ehrlichia canis and Babesia vogeli in dogs in Colombia |
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