The role of DNA damage and caspase activation in cytotoxicity and genotoxicity of macrophages induced by bisphenol-A-glycidyldimethacrylate
Li Y‐C, Kuan Y‐H, Huang F‐M, Chang Y‐C. The role of DNA damage and caspase activation in cytotoxicity and genotoxicity of macrophages induced by bisphenol‐A‐glycidyldimethacrylate. International Endodontic Journal, 45, 499–507, 2012. Aim To evaluate the potential toxicological implications of BisGM...
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| description | Li Y‐C, Kuan Y‐H, Huang F‐M, Chang Y‐C. The role of DNA damage and caspase activation in cytotoxicity and genotoxicity of macrophages induced by bisphenol‐A‐glycidyldimethacrylate. International Endodontic Journal, 45, 499–507, 2012.
Aim To evaluate the potential toxicological implications of BisGMA on murine macrophage cell line RAW264.7.
Methodology Lactate dehydrogenase release, flow cytometry, Western blot and fluorometric assays were used to detect cell viability, mode of cell death and caspase activities, respectively. In addition, alkaline single‐cell gel electrophoresis and cytokinesis‐block micronucleus assays were applied to detect genotoxicity. Statistical analyses were performed using anova followed by the Bonferroni’s t‐test for multi‐group comparisons test.
Results BisGMA demonstrated a cytotoxic effect on RAW264.7 cells in a dose‐dependent and a time‐dependent manner (P |
| doi_str_mv | 10.1111/j.1365-2591.2011.02001.x |
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Aim To evaluate the potential toxicological implications of BisGMA on murine macrophage cell line RAW264.7.
Methodology Lactate dehydrogenase release, flow cytometry, Western blot and fluorometric assays were used to detect cell viability, mode of cell death and caspase activities, respectively. In addition, alkaline single‐cell gel electrophoresis and cytokinesis‐block micronucleus assays were applied to detect genotoxicity. Statistical analyses were performed using anova followed by the Bonferroni’s t‐test for multi‐group comparisons test.
Results BisGMA demonstrated a cytotoxic effect on RAW264.7 cells in a dose‐dependent and a time‐dependent manner (P < 0.05). BisGMA was found to induce two modes of cell death. The mode of cell death changed from apoptosis to necrosis as the concentrations of BisGMA elevated. Caspase‐3, caspase‐8 and caspase‐9 activities were significantly induced by BisGMA in a dose‐dependent manner (P < 0.05). Moreover, BisGMA exhibited genotoxicity via a dose‐related increase in the numbers of micronucleus and DNA strand breaks (P < 0.05).
Conclusions Cytotoxicity and genotoxicity induced by BisGMA are mediated by DNA damage and caspase activation.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/j.1365-2591.2011.02001.x</identifier><identifier>PMID: 22242562</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; apoptosis ; Apoptosis - drug effects ; BisGMA ; Bisphenol A-Glycidyl Methacrylate - toxicity ; Blotting, Western ; Caspase 3 - drug effects ; Caspase 8 - drug effects ; Caspase 9 - drug effects ; caspases ; Caspases - drug effects ; Cell Culture Techniques ; Cell Death - drug effects ; Cell Line ; Cell Survival - drug effects ; Comet Assay ; cytotoxicity ; Cytotoxins - toxicity ; Dental Materials - toxicity ; DNA Damage ; Dose-Response Relationship, Drug ; Enzyme Activation - drug effects ; Enzyme Activators - toxicity ; Flow Cytometry ; Fluorometry ; genotoxicity ; L-Lactate Dehydrogenase - drug effects ; macrophage ; Macrophages - drug effects ; Mice ; Micronucleus Tests ; Mutagens - toxicity ; Necrosis ; Time Factors</subject><ispartof>International endodontic journal, 2012-06, Vol.45 (6), p.499-507</ispartof><rights>2012 International Endodontic Journal</rights><rights>2012 International Endodontic Journal.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4401-b7206ca98b5b693825e27eea90ada4b146df2d841bb0a8b1958e5a978b4cf9b13</citedby><cites>FETCH-LOGICAL-c4401-b7206ca98b5b693825e27eea90ada4b146df2d841bb0a8b1958e5a978b4cf9b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2591.2011.02001.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2591.2011.02001.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22242562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Y.-C.</creatorcontrib><creatorcontrib>Kuan, Y.-H.</creatorcontrib><creatorcontrib>Huang, F.-M.</creatorcontrib><creatorcontrib>Chang, Y.-C.</creatorcontrib><title>The role of DNA damage and caspase activation in cytotoxicity and genotoxicity of macrophages induced by bisphenol-A-glycidyldimethacrylate</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Li Y‐C, Kuan Y‐H, Huang F‐M, Chang Y‐C. The role of DNA damage and caspase activation in cytotoxicity and genotoxicity of macrophages induced by bisphenol‐A‐glycidyldimethacrylate. International Endodontic Journal, 45, 499–507, 2012.
Aim To evaluate the potential toxicological implications of BisGMA on murine macrophage cell line RAW264.7.
Methodology Lactate dehydrogenase release, flow cytometry, Western blot and fluorometric assays were used to detect cell viability, mode of cell death and caspase activities, respectively. In addition, alkaline single‐cell gel electrophoresis and cytokinesis‐block micronucleus assays were applied to detect genotoxicity. Statistical analyses were performed using anova followed by the Bonferroni’s t‐test for multi‐group comparisons test.
Results BisGMA demonstrated a cytotoxic effect on RAW264.7 cells in a dose‐dependent and a time‐dependent manner (P < 0.05). BisGMA was found to induce two modes of cell death. The mode of cell death changed from apoptosis to necrosis as the concentrations of BisGMA elevated. Caspase‐3, caspase‐8 and caspase‐9 activities were significantly induced by BisGMA in a dose‐dependent manner (P < 0.05). Moreover, BisGMA exhibited genotoxicity via a dose‐related increase in the numbers of micronucleus and DNA strand breaks (P < 0.05).
Conclusions Cytotoxicity and genotoxicity induced by BisGMA are mediated by DNA damage and caspase activation.</description><subject>Animals</subject><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>BisGMA</subject><subject>Bisphenol A-Glycidyl Methacrylate - toxicity</subject><subject>Blotting, Western</subject><subject>Caspase 3 - drug effects</subject><subject>Caspase 8 - drug effects</subject><subject>Caspase 9 - drug effects</subject><subject>caspases</subject><subject>Caspases - drug effects</subject><subject>Cell Culture Techniques</subject><subject>Cell Death - drug effects</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Comet Assay</subject><subject>cytotoxicity</subject><subject>Cytotoxins - toxicity</subject><subject>Dental Materials - toxicity</subject><subject>DNA Damage</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Activation - drug effects</subject><subject>Enzyme Activators - toxicity</subject><subject>Flow Cytometry</subject><subject>Fluorometry</subject><subject>genotoxicity</subject><subject>L-Lactate Dehydrogenase - drug effects</subject><subject>macrophage</subject><subject>Macrophages - drug effects</subject><subject>Mice</subject><subject>Micronucleus Tests</subject><subject>Mutagens - toxicity</subject><subject>Necrosis</subject><subject>Time Factors</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhFZCXbBJsJ3aSBYvR0JaiUVFFEUvLPzczHvJH7CmTZ-hL1-mUYYs3vrK_cyyfgxCmJKVxfdylNBM8YbyiKSOUpoQRQtPDC7Q4XbxEC0LzLGFlyc_QG-93hBBOMvoanTHGcsYFW6CHuy3gsW8A9zX-fLPEVrVqA1h1FhvlB-XjbIK7V8H1HXYdNlPoQ39wxoXpCdtA9-8gurTKjP2wjS4-8nZvwGI9Ye38sI1okyyTTTMZZ6fGuhbCNvJTowK8Ra9q1Xh497yfox-XF3erL8n629X1arlOTJ4TmuiCEWFUVWquRZWVjAMrAFRFlFW5prmwNbNlTrUmqtS04iVwVRWlzk1daZqdow9H32Hsf-_BB9k6b6BpVAf93ktKaFHxShAe0fKIxi95P0Ith9G1apwiJOcq5E7Oics5cTlXIZ-qkIcoff_8yl63YE_Cv9lH4NMR-OMamP7bWF5ffJ2nqE-OeucDHE56Nf6SosgKLn_eXMnL2_VtthJCfs8eAQsFqRg</recordid><startdate>201206</startdate><enddate>201206</enddate><creator>Li, Y.-C.</creator><creator>Kuan, Y.-H.</creator><creator>Huang, F.-M.</creator><creator>Chang, Y.-C.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7U7</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>201206</creationdate><title>The role of DNA damage and caspase activation in cytotoxicity and genotoxicity of macrophages induced by bisphenol-A-glycidyldimethacrylate</title><author>Li, Y.-C. ; Kuan, Y.-H. ; Huang, F.-M. ; Chang, Y.-C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4401-b7206ca98b5b693825e27eea90ada4b146df2d841bb0a8b1958e5a978b4cf9b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>BisGMA</topic><topic>Bisphenol A-Glycidyl Methacrylate - toxicity</topic><topic>Blotting, Western</topic><topic>Caspase 3 - drug effects</topic><topic>Caspase 8 - drug effects</topic><topic>Caspase 9 - drug effects</topic><topic>caspases</topic><topic>Caspases - drug effects</topic><topic>Cell Culture Techniques</topic><topic>Cell Death - drug effects</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Comet Assay</topic><topic>cytotoxicity</topic><topic>Cytotoxins - toxicity</topic><topic>Dental Materials - toxicity</topic><topic>DNA Damage</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Activation - drug effects</topic><topic>Enzyme Activators - toxicity</topic><topic>Flow Cytometry</topic><topic>Fluorometry</topic><topic>genotoxicity</topic><topic>L-Lactate Dehydrogenase - drug effects</topic><topic>macrophage</topic><topic>Macrophages - drug effects</topic><topic>Mice</topic><topic>Micronucleus Tests</topic><topic>Mutagens - toxicity</topic><topic>Necrosis</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Y.-C.</creatorcontrib><creatorcontrib>Kuan, Y.-H.</creatorcontrib><creatorcontrib>Huang, F.-M.</creatorcontrib><creatorcontrib>Chang, Y.-C.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Y.-C.</au><au>Kuan, Y.-H.</au><au>Huang, F.-M.</au><au>Chang, Y.-C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of DNA damage and caspase activation in cytotoxicity and genotoxicity of macrophages induced by bisphenol-A-glycidyldimethacrylate</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2012-06</date><risdate>2012</risdate><volume>45</volume><issue>6</issue><spage>499</spage><epage>507</epage><pages>499-507</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Li Y‐C, Kuan Y‐H, Huang F‐M, Chang Y‐C. The role of DNA damage and caspase activation in cytotoxicity and genotoxicity of macrophages induced by bisphenol‐A‐glycidyldimethacrylate. International Endodontic Journal, 45, 499–507, 2012.
Aim To evaluate the potential toxicological implications of BisGMA on murine macrophage cell line RAW264.7.
Methodology Lactate dehydrogenase release, flow cytometry, Western blot and fluorometric assays were used to detect cell viability, mode of cell death and caspase activities, respectively. In addition, alkaline single‐cell gel electrophoresis and cytokinesis‐block micronucleus assays were applied to detect genotoxicity. Statistical analyses were performed using anova followed by the Bonferroni’s t‐test for multi‐group comparisons test.
Results BisGMA demonstrated a cytotoxic effect on RAW264.7 cells in a dose‐dependent and a time‐dependent manner (P < 0.05). BisGMA was found to induce two modes of cell death. The mode of cell death changed from apoptosis to necrosis as the concentrations of BisGMA elevated. Caspase‐3, caspase‐8 and caspase‐9 activities were significantly induced by BisGMA in a dose‐dependent manner (P < 0.05). Moreover, BisGMA exhibited genotoxicity via a dose‐related increase in the numbers of micronucleus and DNA strand breaks (P < 0.05).
Conclusions Cytotoxicity and genotoxicity induced by BisGMA are mediated by DNA damage and caspase activation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22242562</pmid><doi>10.1111/j.1365-2591.2011.02001.x</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals apoptosis Apoptosis - drug effects BisGMA Bisphenol A-Glycidyl Methacrylate - toxicity Blotting, Western Caspase 3 - drug effects Caspase 8 - drug effects Caspase 9 - drug effects caspases Caspases - drug effects Cell Culture Techniques Cell Death - drug effects Cell Line Cell Survival - drug effects Comet Assay cytotoxicity Cytotoxins - toxicity Dental Materials - toxicity DNA Damage Dose-Response Relationship, Drug Enzyme Activation - drug effects Enzyme Activators - toxicity Flow Cytometry Fluorometry genotoxicity L-Lactate Dehydrogenase - drug effects macrophage Macrophages - drug effects Mice Micronucleus Tests Mutagens - toxicity Necrosis Time Factors |
| title | The role of DNA damage and caspase activation in cytotoxicity and genotoxicity of macrophages induced by bisphenol-A-glycidyldimethacrylate |
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